Skip to main content Accessibility help
×
Home
Hostname: page-component-5bf98f6d76-v92w2 Total loading time: 0.401 Render date: 2021-04-20T15:02:36.516Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": false, "newCiteModal": false, "newCitedByModal": true }

L-Arginine attenuates xanthine oxidase and myeloperoxidase activities in hearts of rats during exhaustive exercise

Published online by Cambridge University Press:  08 March 2007

Wan-Teng Lin
Affiliation:
Fu-Jen Catholic University, Department of Nutrition and Food Sciences, TaipeiTaiwan, Republic of China De Lin Institute of Technology, Taipei, Taiwan, Republic of China
Suh-Ching Yang
Affiliation:
School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan, Republic of China
Shiow-Chwen Tsai
Affiliation:
Shin Kong Wu Ho-Su Memorial Hospital, Central Laboratory, Taipei, Taiwan, Republic of China
Chi-Chang Huang
Affiliation:
School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan, Republic of China
Ning-Yuean Lee
Affiliation:
Fu-Jen Catholic University, Department of Nutrition and Food Sciences, TaipeiTaiwan, Republic of China
Corresponding
E-mail address:
Rights & Permissions[Opens in a new window]

Abstract

The present study was to investigate the effects of l-arginine (l-Arg) supplementation on cardiac oxidative stress and the inflammatory response in rats following acute exhaustive exercise on a treadmill. Rats were randomly divided into four groups: sedentary control (SC); SC with l-Arg treatment (SC+Arg); exhaustive exercise (E); exhaustive exercise with l-Arg treatment (E+Arg). Rats in groups SC+Arg and E+Arg received a 2% l-Arg diet. Rats in groups E and E+Arg performed an exhaustive running test on a treadmill at a final speed of 30m/min, 10% grade, at approximately 70–75% VO2max. The results showed a significant increase in cardiac xanthine oxidase (XO) and myeloperoxidase activities and membrane lipid peroxidation endproduct (malondialdehyde; MDA) levels of exercised rats compared with SC rats. The increased cardiac XO activity and MDA levels in exercised rats were significantly decreased in exercised rats supplemented with l-Arg. Myocardial GSSG content increased whereas the GSH:GSSG ratio was depressed in exercised rats compared with SC rats. Cardiac GSSG levels significantly decreased, whereas total glutathione, GSH and the GSH:GSSG ratio increased in exercised rats supplemented with l-Arg compared with exercised rats. The activities of creatinine kinase (CK) and lactate dehydrogenase (LDH), and lactate, uric acid, and nitrite and nitrate levels in the plasma significantly increased in exercised rats compared with SC rats. The activities of plasma CK and LDH were significantly decreased in l-Arg-supplemented plus exercised rats compared with exercised rats. These findings suggest that l-Arg supplementation reduces the oxidative damage and inflammatory response on the myocardium caused by exhaustive exercise in rats.

Type
Research Article
Copyright
Copyright © The Nutrition Society 2006

References

Ashton, T, Rowlands, CC, Jones, E, Young, IS, Jackson, SK, Davies, B, Peters, JRElectron spin resonance spectroscopic detection of oxygen-centered radicals in human serum following exhaustive exercise. Eur J Appl Physiol Occup Physiol (1998) 77 498502CrossRefGoogle Scholar
Au, A, Louch, WE, Ferrier, GR, Howlett, SEL-Arginine ameliorates effects of ischemia and reperfusion in isolated cardiac myocytes. Eur J Pharmacol (2003) 476 4554.CrossRefGoogle ScholarPubMed
Beckam, JS, Koppenol, WHNitric oxide, superoxide, and peroxynitrite: the good, the bad and ugly. Am J Physiol (1996) 271 C1424C1437.Google Scholar
Beers, RFJr, Sizer, IWA spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem (1952) 195 133140.Google ScholarPubMed
Belcastro, AN, Arthur, GD,Albisser, TA, Raj, DAHeart, liver, and skeletal muscle myeloperoxidase activity during exercise. J Appl Physiol (1996) 80 13311335.CrossRefGoogle ScholarPubMed
Brooks, GAWhite, TPDetermination of metabolic and heart rate responses of rats to treadmill exercise. J Appl Physiol (1978) 45 10091015.CrossRefGoogle ScholarPubMed
de Oliveira, SLDiniz, DBAmaya-Farfan, JCarbohydrateenergy restriction may protect the rat brain against oxidative damage and improve physical performance. Br J Nutr (2003) 89 8996.CrossRefGoogle ScholarPubMed
Duarte, JA, Appell, HJ,Carvalho, F, Bastos, ML, Soares, JMEndothelium-derived oxidative stress may contribute to exerciseinduced muscle damage. Int J Sports Med (1993) 14 440443.CrossRefGoogle Scholar
Fielding, RA, Manfredi, T,Ding, W, Fiatarone, MA, Evans, WJ, Cannon, JGAcute phase response in exercise.III. Neutrophil and IL-1 beta accumulation in skeletal muscle. Am J Physiol (1993) 265 R166R172.Google ScholarPubMed
Frankiewicz-Jozko, A, Faff, J, Sieradzan-Gabelska, BChanges in concentrations of tissue free radical marker and serum creatine kinase during the post-exercise period in rats. Eur J Appl Physiol (1996) 74 470474.CrossRefGoogle ScholarPubMed
Fukahori, M, Ichimori, k,Ishida, H, Nakagawa, H, Okino, HNitric oxide reversibly suppresses xanthine oxidase activity. Radic Res (1994) 21 203212.CrossRefGoogle ScholarPubMed
Green, LC, Wagner, DA, Glogowski, J, Skipper, PL, Wishnok, JS, Tannenbaum, SRAnalysis of nitrate, nitrite and [15N]nitrate in biological fluids. Anal Biochem (1982) 126 131138.CrossRefGoogle Scholar
Griffith, OWDetermination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal Biochem (1980) 106 207212.CrossRefGoogle ScholarPubMed
Hassoun, PM, Yu, FS,Zulueta, JJ, White, AC, Lanzillo, JJEffect of nitric oxide and cell redox ststus on the regulation of endothelial cell xanthine dehydrogenase.Am J Physiol (1995) 268 L809L817.Google Scholar
Hellsten, Y, Frandsen, U,Orthenblad, N, Sjodin, B, Richter, EAXanthine oxidase in human skeletal muscle following eccentric exercise: a role in inflammation. J Physiol (1997) 498 239248.CrossRefGoogle ScholarPubMed
Ji, LLAntioxidant enzyme responds to exercise and aging. Med Sci Sports Exerc (1993) 25 225231.CrossRefGoogle Scholar
Ji, LLAntioxidants and oxidative stress in exercise. Proc Soc Exp Biol Med (1999) 222 283292.CrossRefGoogle ScholarPubMed
Ji, LLExercise-induced modulation of antioxidant defense. Ann N Y Acad Sci (2002) 959 8292.CrossRefGoogle ScholarPubMed
Ji, LL, Dillon, D, Wu, EMyocardial aging: antioxdant enzyme systems and related biochemical properties. Am J Physiol (1991) 261 R386R392.Google Scholar
Ji, LL, Mitchell, EWEffects of Adriamycin on heart mitochondrial function in rested and exercised rats. Biochem Pharmacol (1994 47 877885.Google ScholarPubMed
Judge, AR, Dodd, SLXanthine oxidase and activated neutrophils cause oxidative damage to skeletal muscle after contractile claudication. Am J Physiol (2004) 286 H252H256.Google ScholarPubMed
Kihlstrom, M, Ojala, J, Salminen, ADecreased level of cardiac antioxidants in endurance-trained rats. Acta Physiol Scand (1989) 135 549554.CrossRefGoogle ScholarPubMed
Kubes, P, Suzuki, M, Granger, DNModulation of PDF-induced leukocyte adherence and increased microvascular premeability. Am J Physiol (1990) 259 G859G864.Google Scholar
Kubes, P, Suzuki, M, Granger, DNNitric oxide: an endogenous modulator of leukocyte adhesion. PNAS (1991) 88 46514655.CrossRefGoogle ScholarPubMed
Kumar, CT, Reddy, VK,Prasad, M, Thyagaraju, K, Reddanna, PDietary supplementation of vitmin E protects heart tissue from exercise-induced oxidant stress. Mol Cell Biochem (1992) 111 109115.CrossRefGoogle Scholar
Lass, A, Suessenbacher, A,Wolkart, G, Mayer, B, Brunner, FFunctional and analytical evidence for scavenging of oxygen radicals by L-arginine. Mol Pharmacol (2002) 61 10811088.CrossRefGoogle ScholarPubMed
Leeuwenburgh, C, Leichtweis, S,Hollander, J, Fiebig, R, Groe, M, Ji, LLEffect of acute exercise on glutathione deficient heart. Mol Cell Biochem (1996) 156 1724.CrossRefGoogle ScholarPubMed
Leichtweis, SB, Leeuwenburgh, C,Parmelee, DJ, Fiebig, R, Ji, LLRigorous swim training impairs mitochondrial function in post-ischaemic rat heart. Acta Physiol Scand (1997) 160 139148.CrossRefGoogle ScholarPubMed
Lewis, B, Langkamp-Henken, BArginine enhances in vivo immune responses in young, adult and aged mice. J Nutr (2000) 130 18271830.CrossRefGoogle ScholarPubMed
Liu, P, Hock, CE,Nagele, R, Wong, PYFormation of nitric oxide, superoxide, and peroxynitrite in myocardial ischemia-reperfusion-injury in rats. Am J Physiol (1997) 272 H2327H2336.Google ScholarPubMed
Lowry, OH, Rosebrough, NJ,Lewis Farr, A, Randall, RJProtein measurement with the Folin phenol reagent. J Biol Chem (1951) 193 265275.Google ScholarPubMed
McCord, JMOxygen-derived free radicals in postischemic tissue injury. N Engl J Med (1985) 312, 159163.Google ScholarPubMed
Mullane, KM, Kraemer, R, Smith, BMyeloperoxidase activity as a quantitative assessment of neutrophil infiltration into ischemicmyocardium. J Pharmacol Methods (1985) 14 157167.CrossRefGoogle Scholar
Nonami, YThe role of nitric oxide in cardiac ischemia-reperfusion injury. Jpn Circ J (1997) 61 119132.CrossRefGoogle ScholarPubMed
Pabla, R, Buda, AJ,Flynn, DM, Blesse, SA, Shin, AM, Curtis, MJ, Lefer, DJNitric oxide attenuates neutrophil-mediated myocardial contractile dysfunction after ischemia and reperfusion. Circ Res (1996) 78 6572.CrossRefGoogle ScholarPubMed
Qian, ZM, Xiao, DS,Ke, Y, Liao, QKIncreased nitric oxide is one of the causes of changes of iron metabolism in strenuously exercised rats. Am J Physiol (2001) 280 R739R743.Google ScholarPubMed
Radak, Z, Asano, K,Inoue, M, Kizaki, T, Oh-Ishi, S, Suzuki, K, Taniguchi, N, Ohno, HSuperoxide dismutase derivative reduces oxidative damage in skeletal muscle of rats during exhaustive exercise. J Appl Physiol (1995) 79 129135.CrossRefGoogle ScholarPubMed
Radak, Z, Nakamura, A,Nakamoto, H, Asano, K, Ohno, H, Goto, SA period of anaerobic exercise increases the accumulation of reactive carbonyl derivatives in the lungs of rats. Pflugers Arch (1998) 435 439441.Google ScholarPubMed
Reeves, PG, Nielsen, FH, Fahey, GC JrAIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr (1993) 123 19391951.CrossRefGoogle ScholarPubMed
Reid, MBRole of nitric oxide in skeletal muscle: Synthesis, distribution and functional importance. Acta Physiol Scand (1998) 162 401409.CrossRefGoogle ScholarPubMed
Rubbo, H, Radi, R,Trujillo, M, Telleri, R, Kalyanaraman, B, Barnes, S, Kirk, M, Freeman, BANitric oxide regulation of superoxide and peroxylnitrite-dependent lipid peroxidation. Formation of novel nitrogen-containing oxidized lipid derivatives. Biol Chem (1994) 269 2606626075.Google ScholarPubMed
Schaefer, A, Piquard, F,Geny, B, Doutreleau, S, Lampert, E, Mettauer, B, Lonsdorfer, JL-Arginine reduces exercise-induced increase in plasma lactate and ammonia. Int J Sports Med (2002) 23 403407.CrossRefGoogle ScholarPubMed
Schierwagen, C, Bylund-Fellenius, AC, Lundberg, CImproved method for quantification of tissue PMN accumulation measured by myeloperoxidase activity. J Pharmacol Methods (1990) 23 179186.CrossRefGoogle ScholarPubMed
Schmidt, HH, Warner, TD,Nakane, M, Forstermann, U, Murad, FRegulation and subcellular location of nitrogen oxide synthases in RAW264.7 macrophages. Mol Pharmacol (1992) 41 615624.Google ScholarPubMed
Schulz, R, Dodge, KL,Lopaschuk, GD, Clanachan, ASPeroxynitrite impairs cardiac contractile function by decreasing cardiac efficiency. Am J Physiol (1997) 272 H1212H1219.Google ScholarPubMed
Sen, CKAtalay, MHanninen, OExercise-induced oxidative stress: glutathione supplementation and deficiency. J Appl Physiol (1994) 77 21772187.CrossRefGoogle ScholarPubMed
Seward, SW, Seiler, KS, Starnes, JWIntrinsic myocardial function and oxidative stress after exhaustive exercise. J Appl Physiol (1995) 79 251255.CrossRefGoogle ScholarPubMed
Shiono, N, Rao, V, Weisel, RD, Kawasaki, M, Li, RK, Mickle, DAG, Fedak, PWM, Tumiati, LC, Ko, L, Verma, SL-Arginine protects human heart cells from low-volume anoxia and reoxygenation. Am J Physiol 2002) 282 H805H815.Google ScholarPubMed
Somani, SM, Frank, S, Rybak, LPResponses of antioxidant system to acute and trained exercise in rat heart subcellular fractions. Pharmacol Biochem Behav (1995) 51 627634.CrossRefGoogle ScholarPubMed
Tietze, FEnzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem (1969) 27 502522.CrossRefGoogle ScholarPubMed
Tiidus, PMRadical species in inflammation and overtraining. Can J Physiol Pharmacol (1998) 76 533538.CrossRefGoogle ScholarPubMed
Venditti, P, Di Meo, SAntioxidants, tissue damage, and endurance in trained and untrained young male rats. Arch Biochem. Biophys (1996) 331 6368.Google Scholar
Vina, J, Gimeno, A,Sastre, J, Desco, C, Asensi, M, Pallardo, FV, Cuesta, A, Ferrero, JA, Terada, LS, Repine, JEMechanism of free radical production in exhaustive exercise in humans and rats; role of xanthine oxidase and protection by allopurinol. IUBMB Life (2000) 49 539544.Google ScholarPubMed
Wang, BY, Singer, AH,Tsao, PS, Drexler, H, Kosek, J, Cooke, JPDietary arginine prevents atherogenesis in the coronary artery of the hypercholesterolemic rabbit. J Am Coll Cardiol (1994) 23 452458.CrossRefGoogle ScholarPubMed
Wascher, TC, Posch, K,Wallner, S, Hermetter, A, Kostner, GM, Graier, WFVascular effects of L-arginine: anything beyond a substrate for the NO-synthase?. Biochem Biophys Res Commun (1997) 234 3538.CrossRefGoogle ScholarPubMed
Westerfeld, WW, Richert, DA, Higgins, ESFurther studies with xanthine oxidase inhibitors. J Biol Chem (1959) 234 18971900.Google ScholarPubMed
Westing, YHEkblom, BSjodin, BThe metabolic relation between hypoxanthine and uric acid in man following maximal short-distance running. Acta Physiol Scand (1989) 137 341345.CrossRefGoogle ScholarPubMed
Wu, G, Morris, SM JrArginine metabolism: nitric oxide and beyond. Biochem J (1998) 336 117.CrossRefGoogle ScholarPubMed
Xiao, DS, Jiang, L,Che, LL, Lu, LNitric oxide and iron metabolism in exercised rat with L-arginine supplementation. Mol Cell Biochem (2003) 252 6572.CrossRefGoogle ScholarPubMed
Yasmin, W, Strynadka, KD, Schulz, RGeneration of peroxynitrite contributes to ischemia-reperfusion injury in isolated rat hearts. Cardiovasc Res (1997) 33 422432.CrossRefGoogle ScholarPubMed
Zweier, JL, Wang, P, Samouilov, A, Kuppusamy, PEnzymeindependent formation of nitric oxide in biological tissues. Nat Med (1995) 1 804809.CrossRefGoogle ScholarPubMed

Full text views

Full text views reflects PDF downloads, PDFs sent to Google Drive, Dropbox and Kindle and HTML full text views.

Total number of HTML views: 0
Total number of PDF views: 244 *
View data table for this chart

* Views captured on Cambridge Core between September 2016 - 20th April 2021. This data will be updated every 24 hours.

You have Access

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

L-Arginine attenuates xanthine oxidase and myeloperoxidase activities in hearts of rats during exhaustive exercise
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

L-Arginine attenuates xanthine oxidase and myeloperoxidase activities in hearts of rats during exhaustive exercise
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

L-Arginine attenuates xanthine oxidase and myeloperoxidase activities in hearts of rats during exhaustive exercise
Available formats
×
×

Reply to: Submit a response


Your details


Conflicting interests

Do you have any conflicting interests? *