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Konjac glucomannan polysaccharide and inulin oligosaccharide enhance the colonic mucosal barrier function and modulate gut-associated lymphoid tissue immunity in C57BL/6J mice

  • Chih-Hsuan Changchien (a1) (a2), Yi-Chun Han (a3) and Hsiao-Ling Chen (a4) (a5)


Both konjac glucomannan (KGM) and inulin oligosaccharide have been shown to improve bowel function, but their effects on the mucosal barrier function and immunity are not fully understood. The aim of the present study was to determine the effects of a low-level supplementation of dietary fibres on the colonic mucosal barrier function, antioxidant enzyme defence and immunity. C57BL/6J mice (6 weeks of age, eight per group) were randomly assigned to consume one of the following diets: control or control diet supplemented with 2 % (w/w) of KGM, inulin oligosaccharide (degree polymerisation = 8) or KGM+inulin (1 %, w/w each (K+I)). Fresh faeces were collected on days 19–21. Mice were killed on day 22 after fasting. Segments of colon tissues were processed for histological procedure and stained for acidic mucins and tight junction protein marker zona occludin-1 (ZO-1). The remaining tissues were processed to determine the gene expression of mucin 2, tight junction proteins, antioxidant enzymes and cytokines. The plasma cytokines were measured. Results indicated that KGM, inulin and K+I significantly increased the mucosal layer thickness, mucin density (granule number/crypt) and gene expression of Muc2 as compared with the control. All fibre treatments increased the gene expressions of ZO-1, occludin, glutathione peroxidase, glutathione S-transferase π, catalase and IL-10. In addition, all fibre treatments increased faecal butyrate and probiotics, and plasma IL-10 concentrations. In conclusion, supplementation of low-level, 2 % (w/w), of K+I was sufficient to enhance the mucosal barrier function and anti-inflammatory status.


Corresponding author

*Corresponding author: Hsiao-Ling Chen, fax +886-4-23321215, email


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1. Aureli, P, Capurso, L, Castellazzi, AM, et al. (2011) Probiotics and health: an evidence-based review. Pharmacol Res 63, 366376.
2. Wu, WT & Chen, HL (2011) Konjac glucomannan and inulin systematically modulate antioxidant defense in rats fed a high-fat fiber-free diet. J Agri Food Chem 59, 91949200.
3. Kudelka, MR, Ju, T, Heimburg-Molinaro, J, et al. (2015) Simple sugars to complex disease–mucin-type O-glycans in cancer. Adv Cancer Res 126, 53135.
4. Kim, YS & Ho, SB (2010) Intestinal goblet cells and mucins in health and disease: recent insights and progress. Curr Gastroenterol Rep 12, 319330.
5. Rousseau, K, Byrne, C, Kim, YS, et al. (2004) The complete genomic organization of the human MUC6 and MUC2 mucin genes. Genomics 83, 936939.
6. Baldus, SE & Hanisch, F-G (2000) Biochemistry and pathological importance of mucin-associated antigens in gastrointestinal neoplasia. Adv Cancer Res 79, 201248.
7. Camilleri, M (2019) Leaky gut: mechanisms, measurement and clinical implications in humans. Gut 68, 15161526.
8. Van Itallie, CM & Anderson, JM (2014) Architecture of tight junctions and principles of molecular composition. Semin Cell Dev Biol 36, 157165.
9. Seril, DN, Liao, J, Yang, GY, et al. (2003) Oxidative stress and ulcerative colitis-associated carcinogenesis: studies in humans and animal models. Carcinogenesis 24, 353362.
10. Babbs, CF (1990) Free radicals and the etiology of colon cancer. Free Rad Biol Med 8, 191200.
11. Seifert, S & Watzl, B (2007) Inulin and oligofructose: review of experimental data on immune modulation. J Nutr 137, 2563s2567s.
12. Doi, K (1995) Effect of konjac fibre (glucomannan) on glucose and lipids. Eur J Clin Nutr 49, Suppl. 3, S190S197.
13. Pool-Zobel, B, van Loo, J, Rowland, I, et al. (2002) Experimental evidences on the potential of prebiotic fructans to reduce the risk of colon cancer. Br J Nutr 87, Suppl. 2, S273S281.
14. Chen, HL, Lin, YM & Wang, YC (2010) Comparative effects of cellulose and soluble fibers (pectin, konjac glucomannan, inulin) on fecal water toxicity toward Caco-2 cells, fecal bacteria enzymes, bile acid, and short-chain fatty acids. J Agri Food Chem 58, 1027710281.
15. Chen, H-L, Cheng, H-C, Wu, W-T, et al. (2008) Supplementation of konjac glucomannan into a low-fiber Chinese diet promoted bowel movement and improved colonic ecology in constipated adults–a placebo-controlled, diet-controlled trial. J Am Coll Nutr 27, 102108.
16. Council of Agriculture, Executive Yuan (2018) Animal Protective Act of Taiwan. Taipei, Taiwan: Council of Agriculture.
17. National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals (2011) Guide for the Care and Use of Laboratory Animals, 8th ed. Washington, DC: National Academies Press.
18. Reeves, PG, Nielsen, FH & Fahey, GC Jr. (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123, 19391951.
19. Lison, L (1954) Alcian blue 8 G with chlorantine fast red 5 B. A technic for selective staining of mucopolysaccharides. Stain Technol 29, 131138.
20. Jansen, GJ, Wildeboer-Veloo, AC, Tonk, RH, et al. (1999) Development and validation of an automated, microscopy-based method for enumeration of groups of intestinal bacteria. J Microbiol Methods 37, 215221.
21. Nagahama, M, Nagayasu, K, Kobayashi, K, et al. (2002) Binding component of Clostridium perfringens iota-toxin induces endocytosis in Vero cells. Infect Immun 70, 19091914.
22. Wang, RF, Cao, WW & Cerniglia, CE (1996) PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples. Appl Environ Microbiol 62, 12421247.
23. Xue, Z, Yu, J, Zhao, M, et al. (2017) Effects of synbiotics on intestinal mucosal barrier in rat model. Clin Nutr Exp 13, 1221.
24. Pastorelli, L, De Salvo, C, Mercado, JR, et al. (2013) Central role of the gut epithelial barrier in the pathogenesis of chronic intestinal inflammation: lessons learned from animal models and human genetics. Front Immunol 4, 280280.
25. Emenaker, NJ & Basson, MD (2001) Short chain fatty acids differentially modulate cellular phenotype and c-myc protein levels in primary human nonmalignant and malignant colonocytes. Digest Dis Sci 46, 96105.
26. Hague, A, Butt, AJ & Paraskeva, C (1996) The role of butyrate in human colonic epithelial cells: an energy source or inducer of differentiation and apoptosis? Proc Nutr Soc 55, 937943.
27. Wu, W-T, Yang, L-C & Chen, H-L (2014) Effects of konjac glucomannan, inulin and cellulose on acute colonic responses to genotoxic azoxymethane. Food Chem 155, 304310.
28. Gill, HS (2003) Probiotics to enhance anti-infective defences in the gastrointestinal tract. Best Pract Res Clin Gastroenterol 17, 755773.
29. Mack, DR, Michail, S, Wei, S, et al. (1999) Probiotics inhibit enteropathogenic E. coli adherence in vitro by inducing intestinal mucin gene expression. Am J Physiol 276, G941G950.
30. Gaudier, E, Rival, M, Buisine, MP, et al. (2009) Butyrate enemas upregulate Muc genes expression but decrease adherent mucus thickness in mice colon. Physiol Res 58, 111119.
31. Wang, HB, Wang, PY, Wang, X, et al. (2012) Butyrate enhances intestinal epithelial barrier function via up-regulation of tight junction protein Claudin-1 transcription. Dig Dis Sci 57, 31263135.
32. Bordin, M, D’Atri, F, Guillemot, L, et al. (2004) Histone deacetylase inhibitors up-regulate the expression of tight junction proteins. Mol Cancer Res 2, 692701.
33. Gourbeyre, P, Denery, S & Bodinier, M (2011) Probiotics, prebiotics, and synbiotics: impact on the gut immune system and allergic reactions. J Leukoc Biol 89, 685695.
34. Markowiak, P & Śliżewska, K (2017) Effects of probiotics, prebiotics, and synbiotics on human health. Nutrients 9, 1021.
35. Kengatharan, M, De Kimpe, SJ & Thiemermann, C (1996) Analysis of the signal transduction in the induction of nitric oxide synthase by lipoteichoic acid in macrophages. Br J Pharmacol 117, 11631170.
36. Azad, MAK, Sarker, M & Wan, D (2018) Immunomodulatory effects of probiotics on cytokine profiles. Biomed Res Int 8063647.
37. Moore, KW, Malefyt, RdW, Coffman, RL, et al. (2001) Interleukin-10 and the interleukin-10 receptor. Ann Rev Immunol 19, 683765.
38. Roller, M, Pietro Femia, A, Caderni, G, et al. (2004) Intestinal immunity of rats with colon cancer is modulated by oligofructose-enriched inulin combined with Lactobacillus rhamnosus and Bifidobacterium lactis . Br J Nutr 92, 931938.
39. Viladomiu, M, Hontecillas, R, Yuan, L, et al. (2013) Nutritional protective mechanisms against gut inflammation. J Nutr Biochem 24, 929939.
40. Arenas-Padilla, M, Duarte-Gutierrez, JL & Mata-Haro, V (2018) Bifidobacterium animalis ssp. lactis Bb12 induces IL-10 through cell membrane-associated components via TLR2 in swine. J Appli Microbiol (epublication ahead of print 14 August 2018).
41. Yeh, S-L, Wu, T-C, Chan, S-T, et al. (2014) Fructo-oligosaccharide attenuates the production of pro-inflammatory cytokines and the activation of JNK/Jun pathway in the lungs of d-galactose-treated Balb/cJ mice. Eur J Nutr 53, 449456.
42. Xie, LM, Ge, YY, Huang, X, et al. (2015) Effects of fermentable dietary fiber supplementation on oxidative and inflammatory status in hemodialysis patients. Int J Clin Exp Med 8, 13631369.


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Konjac glucomannan polysaccharide and inulin oligosaccharide enhance the colonic mucosal barrier function and modulate gut-associated lymphoid tissue immunity in C57BL/6J mice

  • Chih-Hsuan Changchien (a1) (a2), Yi-Chun Han (a3) and Hsiao-Ling Chen (a4) (a5)


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