Skip to main content Accessibility help
×
Home

Kinetics of large ciliate protozoa in the rumen of cattle given sugar cane diets

  • R. A. Leng (a1), M. Gill (a2), T. J. Kempton (a1), J. B. Rowe (a2), J. V. Nolan (a1), S. J. Stachiw (a1) and T. R. Preston (a2)...

Abstract

1. Experiments were undertaken to examine the kinetics of large ciliate protozoa in the rumen of cattle on sugar-cane diets.

2. Three Zebu bulls were fed once daily on a diet of sugar cane and wheat bran. The diurnal patterns of volatile fatty acids and ammonia concentrations, and the numbers of protozoa in rumen fluid were determined. The numbers of protozoa reached values of 5 × 104/ml for holotrichs (large ciliates) mainly Isoiricha and Dasytricha spp and 4 × 105 for smaller protozoa, mainiy Entodinia (small ciliates)

3. A method was developed which allowed large ciliate protozoa in rumen fluid to be separated from plant material and bacteria and concentrated in a relatively uncontaminated form. Analysis of these protozoa indicated that 1.8 × 105 large ciliates contained 1 mg nitrogen and approximately 32 mg dry matter

4. A labelled preparation consisting mainly of large ciliates (principally Isotricha spp.) was obtained by incubating isolated protozoa in rumen fluid (free of plant materials) containing [14C-methyl]choline and then isolating them by sedimentation and differential centrifugation

5. A portion of the preparation containing labelled protozoa was incubated in vitro with rumen fluid to determine the turnover of 14C-labelled metabolites. There was no apparent dilution of the label in the protozoa over a 22 h period

6. A major portion of the preparation containing labelled protozoa was returned to the rumen of each of the donor cattle as a single injection. The specific radioactivity in the large protozoa (μCi/mg N) was monitored frequently for over 30 h, and thereafter daily for a further 12 d. The kinetics of tracer dilution were analyzed to give estimates of the size of the pool of these large ciliates in the rumen (24–46 g N), and of their apparent rate of turnover

7. In contrast to the slow turnover of the large ciliates, the rate of turnover of the rumen fluid pool (approximately 54 l), estimated from the rate of dilution of polyethylene glycol, was considerably faster. Large ciliates were therefore selectively retained within the rumen

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Kinetics of large ciliate protozoa in the rumen of cattle given sugar cane diets
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Kinetics of large ciliate protozoa in the rumen of cattle given sugar cane diets
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Kinetics of large ciliate protozoa in the rumen of cattle given sugar cane diets
      Available formats
      ×

Copyright

References

Hide All
Annison, E. F. & White, R. R. (1961). Biochem. J. 80, 162.
Bergen, W. G. & Yokoyama, M. T. (1977). J. Anim. Sci. 46, 573.
Bird, S. H., Baigent, D. R., Dixon, R. M. & Leng, R. A. (1978). Proc. Soc. Anim. Prod. 12, 137.
Bird, S. H., Hill, M. K. & Leng, R. A. (1979). Br. J. Nutr. 42, 81.
Bird, S. H. & Leng, R. A. (1978). Br. J. Nutr. 40, 163.
Broad, T. E. & Dawson, R. M. C. (1976). J. gen. Microb. 92, 391.
Bruno, G. A. & Christian, J. E. (1961). Analyt. Chem. 33, 650.
Bygrave, F. L. & Dawson, R. M. C. (1976). Biochem. J. 160, 481.
Clarke, R. T. J. (1965). N.Z. Soc. Anim. Prod. 25, 96.
Coleman, G. S. (1975). In Digestion and Metabolism in the Ruminant, p. 149 [McDonald, I. W. and Warner, A. C. I., editors]. Armidale: University of New England Publishing Unit.
Coleman, G. S. (1979). Trop. Anim. Prod. 4, 199.
Coleman, G. S., Dawson, R. M. C. & Grime, D. W. (1980). Proc. Nutr. Soc. 39, 6A.
Elliott, R., Ferreiro, H. M. & Priego, A. (1978). Trop. Anim. Prod. 3, 36.
Erwin, E. S., Marco, G. J. & Emery, E. M. (1961). J. Dairy Sci. 44, 1768.
Harrison, D. G., Beever, D. E. & Osbourn, D. F. (1979). Br. J. Nutr. 41, 521.
Leng, R. A. (1976). In Reviews in Rural Science, vol. 2, p. 85 [Sutherland, T. M., McWilliam, J. R. and Leng, R. A., editors]. Armidale: University of New England Publishing Unit.
Malawar, S. J. & Powell, D. W. (1967). Gastroenterology 53, 250.
Minor, S., MacLeod, N. A., Preston, T. R. & Leng, R. A. (1977). Trop. Anim. Prod. 2, 163.
Neill, A. R., Grime, D. W. & Dawson, R. M. C. (1978). Biochem. J. 170, 529.
Neill, A. R., Grime, D. W., Snoswell, A. M., Northrop, A. J., Lindsay, D. B. & Dawson, R. M. C. (1979). Biochem. J. 180, 559.
Nolan, J. V. & Leng, R. A. (1972). Br. J. Nutr. 27, 177.
Singh, U. B., Verma, D. N., Varma, A. & Ranjhan, S. K. (1974). J. Dairy Res. 41, 299.
Ulbrich, M. & Scholz, H. (1966). Arch. Tierernahr. 16, 327.
Valdez, R. E., Alvarez, F. J., Ferreiro, H. M., Guerra, F., Lopez, J., Priego, A., Blackburn, T. H., Leng, R. A. & Preston, T. R. (1977). Trop. Anim. Prod. 2, 260.
Van Slyke, D. D. & Folch, J. (1940). J. biol. Chem. 136, 509.
Warner, A. C. I. (1962). J. gen. Microbiol. 28, 129.
Weller, R. A. & Pilgrim, A. F. (1974). Br. J. Nutr. 32, 341.

Kinetics of large ciliate protozoa in the rumen of cattle given sugar cane diets

  • R. A. Leng (a1), M. Gill (a2), T. J. Kempton (a1), J. B. Rowe (a2), J. V. Nolan (a1), S. J. Stachiw (a1) and T. R. Preston (a2)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed