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Inhibitory effect of yoghurt and soya yoghurt containing bifidobacteria on the proliferation of Ehrlich ascites tumour cells in vitro and in vivo in a mouse tumour model

Published online by Cambridge University Press:  09 March 2007

I. A. Abd El-Gawad ,
E. M. El-Sayed ,
S. A. Hafez ,
H. M. El-Zeini  and
F. A. Saleh
I. A. Abd El-Gawad*
Affiliation:
Dairy Science and Technology Department, Faculty of Agriculture, Cairo University, PO 12613, Giza, Egypt
E. M. El-Sayed
Affiliation:
Dairy Science and Technology Department, Faculty of Agriculture, Cairo University, PO 12613, Giza, Egypt
S. A. Hafez
Affiliation:
Special Food and Technology Department, Food Technology Research Institute, Agriculture Research Center, Giza, Egypt
H. M. El-Zeini
Affiliation:
Dairy Science and Technology Department, Faculty of Agriculture, Cairo University, PO 12613, Giza, Egypt
F. A. Saleh
Affiliation:
Special Food and Technology Department, Food Technology Research Institute, Agriculture Research Center, Giza, Egypt
*
*Corresponding author: fax +20 2 7743824, Email ibrahim_gawad@hotmail.com
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Abstract

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The effect of yoghurt and soya yoghurt containing Bifidobacterium lactis Bb-12 or B. longum Bb-46 on Ehrlich ascites tumour cell proliferation was investigated in vitro and in vivo. Tumour cells were incubated with B. lactis Bb-12 or B. longum Bb-46 cultivated in de Mann Rogosa Sharpe (MRS) broth medium, or with their centrifuged supernatant fractions or sediments, for 2 h at 37°C. Treatment resulted in the inhibition of tumour cell proliferation by 85·42 (sd 0·78) and 85·10 (sd 1·28) % by intact micro-organisms, 77·61 (sd 0·29) and 71·43 (sd 1·75) % by their supernatant fractions, but only 4·00 (sd 0·19) and 9·09 (sd 1·24) % by the two sedimented bacteria, respectively. The incubation of tumour cells with yoghurt and soya yoghurt containing Bb-12 for 2 h resulted in 83·01 (sd 0·11) and 88·23 (sd 0·06) % inhibition, respectively, while it was 83·82 (sd 0·24) and 86·36 (sd 0·06) %, respectively for the same products containing Bb-46. Corresponding values for plain yoghurt and soya milk (without bifidobacteria) were 32·81 (sd 0·14) and 5·55 (sd 0·12) %, respectively. The differences between yoghurt or soya yoghurt containing Bb-12 or Bb-46 and plain yoghurt, soya milk or control treatments were statistically significant (n 3; P>0·05). Female Swiss albino mice were injected intraperitoneally with the same tumour cells. The lifespan of mice fed diets supplemented with yoghurt or soya yoghurt containing Bb-12 or Bb-46 was prolonged by 16, 23, 34 and 39%, respectively compared with that of the positive control group (n 6; P>0·05). The lifespan of groups fed plain yoghurt or soya milk was prolonged by 15 and 8%, respectively. Prolongation of lifespan was positively correlated with faeces bifidobacterial count in the groups fed yoghurt or soya yoghurt containing bifidobacteria (r 0·917; P>0·05).

Keywords

YoghurtSoya yoghurtEhrlich ascites tumour cellsBifidobacteria
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 1 , July 2004 , pp. 81 - 86
Copyright
Copyright © The Nutrition Society 2004

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