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Inhibitory effect of high protein intake on nephrocalcinogenesis in female rats

Published online by Cambridge University Press:  09 March 2007

J. G. H. Sterck ,
J. Ritskes-Hoitinga  and
A. C. Beynen
J. G. H. Sterck
Affiliation:
Department of Human Nutrition, Agricultural University, PO Box 8129, 6700 EV Wageningen Department of Laboratory Animal Science, State University, PO Box 80.166, 3508 TD Utrecht, The Netherlands
J. Ritskes-Hoitinga
Affiliation:
Department of Laboratory Animal Science, State University, PO Box 80.166, 3508 TD Utrecht, The Netherlands
A. C. Beynen
Affiliation:
Department of Human Nutrition, Agricultural University, PO Box 8129, 6700 EV Wageningen Department of Laboratory Animal Science, State University, PO Box 80.166, 3508 TD Utrecht, The Netherlands
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Abstract

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Increased intakes of protein have been shown to reduce kidney calcification (nephrocalcinosis) in female rats. Two questions were addressed in the present study. First, can protein-induced inhibition of nephrocalcinosis be demonstrated when the diets used are balanced for calcium, magnesium and phosphorus in the added protein? Second, can the protein effect be explained by the frequently observed magnesiuria after giving high-protein diets? Nephrocalcinosis was induced in female rats by giving purified diets containing 151 g casein/kg and either an increased concentration of P (6 v. 2 g/kg) or a decreased concentration of Mg (0·1 v. 0·4 g/kg). To these diets 151 g ovalbumin/kg was added at the expense of glucose, and the diets were balanced for Ca, Mg and P in ovalbumin. The diets were given for 29 d. In rats fed on the diet containing 151 g protein/kg, an increased intake of P or a decreased intake of Mg caused nephrocalcinosis as measured chemically by analysis of kidney Ca as well as histologically by scoring kidney sections stained according to Von Kossa's method. The addition of ovalbumin to the diet prevented the induction of nephrocalcinosis. High P intake and low Mg intake with the low-protein diets induced enhanced loss of albumin in urine, suggesting that nephrocalcinosis caused kidney damage. Increased protein intake with a non-calcinogenic diet also caused increased albumin excretion in urine. Irrespective of the composition of the background diet, increased protein intake caused increased urinary excretion of Mg. When all dietary groups were considered, differences in nephrocalcinosis and urinary Mg output were not proportionally related.

Nephrocalcinosis: Phosphorus: Magnesium: Protein: Rat

Keywords

NephrocalcinosisPhosphorusMagnesiumProteinRat
Type
Metabolism Effects of Diet
Information
British Journal of Nutrition , Volume 67 , Issue 2 , March 1992 , pp. 223 - 233
Copyright
Copyright © The Nutrition Society 1992

References

REFERENCES

Al-Modhefer, A. K. J., Atherton, J. C., Garland, H. O., Singh, H. J. & Walker, J. (1986) Kidney function in rats with corticomedullary nephrocalcinosis: effects of alterations in dietary calcium and magnesium. Journal of Physiology 380, 405–414.CrossRefGoogle ScholarPubMed
Balazs, T. & Roepke, R. R. (1966) Lysozymuria induced in rats by nephrotoxic agents. Proceedings of the Society for Experimental Biology and Medicine 123, 380–385.CrossRefGoogle ScholarPubMed
Bouby, N., Trinh-Trang-Tan, M. M., Laouari, D., Kleinknecht, C., Grünfeld, J. P., Kriz, W. & Bankir, L. (1988) Role of the urinary concentrating process in the renal effects of high protein intake. Kidney International 34, 4–12.CrossRefGoogle ScholarPubMed
Boulet, M., Marier, J. R. & Rose, D. (1962) Effect of magnesium on formation of calcium phosphate precipitates. Archives of Biochemistry and Biophysics 96, 629–636.CrossRefGoogle ScholarPubMed
Bunce, G. E. & Bloomer, J. E. (1972) Effect of magnesium deficiency on serum and urinary ions in rats: studies with ion-selective electrodes. Journal of Nutrition 102, 863–872.CrossRefGoogle ScholarPubMed
Bunce, G. E., Sauberlich, H. E., Reeves, P. G. & Oba, T. S. (1965) Dietary phosphorus and magnesium deficiency in the rat. Journal of Nutrition 86, 406–414.CrossRefGoogle ScholarPubMed
Draper, H. H., Sie, T. L. & Bergan, J. G. (1972) Osteoporosis in aging rats induced by high phosphorus diets. Journal of Nutrition 102, 1133–1142.CrossRefGoogle ScholarPubMed
Du Bruyn, D. B. (1972) Nephrocalcinosis in the white rat. Part 2. The relationship between dietary magnesium, calcium and phosphorus content and kidney calcification and bone magnesium. South African Medical Journal 46, 1588–1593.Google Scholar
Eklund, A, Agren, G., Nordgren, H. & Stenram, U. (1973) Nephrocalcinosis in adolescent Sprague-Dawley rats fed casein and different salt mixtures. Nutrition and Metabolism 15, 348–356.CrossRefGoogle ScholarPubMed
Goulding, A. & Malthus, R. S. (1969) Effect of dietary magnesium on the development of nephrocalcinosis in rats. Journal of Nutrition 97, 353–358.CrossRefGoogle ScholarPubMed
Harrison, J. F., Parker, R. W. & De Silva, K. L. (1973) Lysozymuria and acute disorders of renal function. Journal of Clinical Pathology 26, 278–284.CrossRefGoogle ScholarPubMed
Harwood, E. J. (1982) The influence of dietary magnesium on reduction of nephrocalcinosis in rats fed purified diets. Laboratory Animals 16, 314–318.CrossRefGoogle Scholar
HellersteinE., E. E., E., Vitale, J. J., White, P. L., Hegsted, D. M., Zamcheck, N. & Nakamura, M. (1957) Influence of dietary magnesium on cardiac and renal lesions of young rats fed an atherogenic diet. Journal of Experimental Medicine 106, 767–776.CrossRefGoogle ScholarPubMed
Hitchman, A. J., Hasany, S. A., Hitchman, A., Harrison, J. E. & Tam, C. (1979) Phosphate-induced renal calcification in the rat. Canadian Journal of Physiology and Pharmacology 57, 92–97.CrossRefGoogle ScholarPubMed
Hoek, A. C., Lemmens, A. G., Mullink, J. W. M. A. & Beynen, A. C. (1988) Influence of dietary calcium: phosphorus ratio on mineral excretion and nephrocalcinosis in female rats. Journal of Nutrition 118, 1210–1216.CrossRefGoogle Scholar
Kang, S. S., Price, R. G., Yudkin, J., Worcester, N. A. & Bruckdorfer, K. R. (1979) The influence of dietary carbohydrate and fat on kidney calcification and the urinary excretion of N-acetyl-β-glucosaminidase. British Journal of Nutrition 41, 65–71.CrossRefGoogle ScholarPubMed
Kaunitz, H. & Johnson, R. E. (1976) Dietary protein, fat and minerals in nephrocalcinosis in female rats. Metabolism 25, 69–77.CrossRefGoogle ScholarPubMed
Mallory, F. B. (1961) Pathological Technique. New York: Hafner Publishing Co.Google Scholar
Mancini, G., Carbonara, A. O. & Heremans, J. F. (1965) Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2, 235–239.CrossRefGoogle ScholarPubMed
MarsY, W. H. M. Y, W. H. M., Lemmens, A. G. & Beynen, A. C. (1988) Dietary phosphorus and nephrocalcinosis in female rats. Nutrition Reports International 38, 249–258.Google Scholar
Massry, S. G. & Glassock, R. J. (1983) Textbook of Nephrology. Baltimore: Williams and Wilkins.Google Scholar
Osserman, E. F. & Lawlor, D. P. (1966) Serum and urinary lysozyme (muramidase) in monocytic and monomyelocytic leukemia. Journal of Experimental Medicine 124, 921–951.CrossRefGoogle ScholarPubMed
Parker, H. E. (1985) Magnesium and phosphorus interrelationships at deficient to normal dietary levels. Nutrition Reports International 32, 983–990.Google Scholar
Ritskes-Hoitinga, J., Lemmens, A. G. & Beynen, A. C. (1989) Nutrition and kidney calcification in rats. Laboratory Animals 23, 313–318.CrossRefGoogle ScholarPubMed
Rosenberg, M. E., Swanson, J. E., Thomas, B. L., Chmielewski, D. & Hostetter, T. H. (1988) Dietary protein restriction lessens the glomerular permselective defect in human renal disease. Contributions to Nephrology 68, 157–165.CrossRefGoogle ScholarPubMed
Schneider, W. & Menden, E. (1988) Der Einflu β langfristig überhöhter Proteinzufuhr auf der Mineralstoffwechsel und die Nierenfunktion der Ratte. I. Die renale und enterale Ausscheidung von Calcium, Magnesium, Phosphor, Sulfat und Säure. (The long-term influence of increased protein supply on mineral metabolism and kidney function in the rat. I. Renal and intestinal elimination of calcium, magnesium, phosphorus, sulphate and acids.) Zeitschrift, für Ernährungswissenschaft 27, 170–185.CrossRefGoogle Scholar
Stonard, M. D., Samuels, D. M. & Lock, E. A. (1984) The pathogenesis and effect on renal function of nephrocalcinosis induced by different diets in female rats. Food and Chemical Toxicology 22, 139–146.CrossRefGoogle ScholarPubMed
Taussky, H. H. & Shorr, E. (1953) A microcolorimetric method for the determination of inorganic phosphorus. Journal of Biological Chemistry 202, 675–685.CrossRefGoogle ScholarPubMed