Skip to main content Accessibility help
×
Home

Influence of disodium malate on microbial growth and fermentation in rumen-simulation technique fermenters receiving medium- and high-concentrate diets

  • J. A. Gómez (a1), M. L. Tejido (a1) and M. D. Carro (a1)

Abstract

Two incubation trials were carried out with the rumen-simulation technique (RUSITEC). In each trial, four vessels received a diet of grass hay and concentrate (600 and 400 g/kg DM, respectively; diet F), and the other four were fed a diet composed of concentrate and barley straw (900 and 100 g/kg DM, respectively; Diet C). Vessels were given 20 g of the corresponding diet daily, and half of them were supplemented with disodium malate to achieve a final concentration of 6.55 mM. There were no effects (P>0·05) of malate either on pH or on the daily production of NH3-N, but malate treatment increased (P<0·05) DM, neutral detergent and acid detergent fibre disappearance after 48 h incubation. The daily production of propionate and butyrate increased (P<0·001), and the ratio CH4:volatile fatty acids decreased (P<0·001) by supplementing both diets with malate. Whereas adding malate to the F diet produced an increase in acetate production (P=0·011) and the growth of solid-associated micro-organisms (P=0·037), no effects (P>0·05) were observed for diet C. For both diets, there were no differences (P>0·05) between treatments in the daily flow of liquid-associated micro-organisms measured using 15N as a microbial marker. These results indicate that malate stimulated the in vitro fermentation of both diets by increasing the apparent disappearance of the diet and decreasing the ratio of CH4:volatile fatty acids, but a greater response was observed with diet F. If these results are confirmed in vivo, malate could be used as a feed additive for ruminants fed diets containing medium proportions of forage (i.e. dairy animals) and not only in animals fed high-concentrate diets, as has so far been proposed.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Influence of disodium malate on microbial growth and fermentation in rumen-simulation technique fermenters receiving medium- and high-concentrate diets
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Influence of disodium malate on microbial growth and fermentation in rumen-simulation technique fermenters receiving medium- and high-concentrate diets
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Influence of disodium malate on microbial growth and fermentation in rumen-simulation technique fermenters receiving medium- and high-concentrate diets
      Available formats
      ×

Copyright

Corresponding author

*Corresponding author: Dr M. D. Carro, fax +34 987 291311, email DP1MCT@UNILEON.ES

References

Hide All
Association of Official Analytical Chemists (1995) Official Methods of Analysis 16th edn Arlington, VA AOAC
Barrie, S & Workman, CT (1984) An automated analytical system for nutritional investigations using N-15 tracers. Spectrosc-In J 3, 439447.
Callaway, TR & Martin, SA (1996) Effects of organic acid and monensin treatment on in vitro mixed ruminal microorganism fermentation of cracked corn. J Anim Sci 74, 19821989.
Callaway, TR & Martin, SA (1997) Effects of cellobiose and monensin on in vitro fermentation of organic acids by mixed ruminal bacteria. J Dairy Sci 80, 11261135.
Callaway, TR, Martin, SA, Wampler, JL, Hill, NS & Hill, GM (1997) Malate content of forage varieties commonly fed to cattle. J Dairy Sci 80, 16511655.
Carro, MD & Miller, EL (1999) Effect of supplementing a fibre basal diet with different nitrogen forms on ruminal fermentation and microbial growth in an in vitro semicontinuous culture system (RUSITEC). Br J Nutr 82, 149157.
Carro, MD & Ranilla, MJ (2003a) Effect of the addition of malate on in vitro rumen fermentation of cereal grains. Br J Nutr 89, 279288.
Carro, MD & Ranilla, MJ (2003b) Influence of different concentrations of disodium fumarate on methane production and fermentation of concentrate feeds by rumen microorganisms in vitro. Br J Nutr 90, 617623.
Carro, MD, Lebzien, P & Rohr, K (1992) Influence of yeast culture on the in vitro fermentation (Rusitec) of diets containing variable portions of concentrates. Anim Feed Sci Technol 37, 209220.
Carro, MD, López, S, Valdés, C & Ovejero, FJ (1999) Effect of DL-malate on mixed ruminal microorganism fermentation using the rumen simulation technique (RUSITEC). Anim Feed Sci Technol 79, 279288.
Czerkawski, JW (1986) An Introduction to Rumen Studies Oxford Pergammon Press
Czerkawski, JW & Breckenridge, G (1977) Design and development of a long-term rumen simulation technique (Rusitec). Br J Nutr 38, 371384.
Demeyer, DI & Henderickx, MK (1967) Competitive inhibition of in vitro methane production by mixed rumen bacteria. Arch Int Phys Bioch 75, 157159.
Eun, J-S, Fellner, V & Gumpertz, ML (2004) Methane production by mixed ruminal cultures incubated in dual-flow fermenters. J Dairy Sci 87, 112121.
López, S, Valdés, C, Newbold, CJ & Wallace, RJ (1999) Influence of sodium fumarate addition on rumen fermentation in vitro. Br J Nutr 81, 5964.
McAllister, TA, Bae, HD, Jones, GA & Cheng, KJ (1994) Microbial attachment and feed digestion in the rumen. J Anim Sci 72, 30043018.
McDougall, EI (1948) Studies on ruminant saliva. I. The composition and output of sheep's saliva. Biochem J 43, 99109.
Martin, SA (1998) Manipulation of ruminal fermentation with organic acids: a review. J Anim Sci 76, 31233132.
Martin, SA (2004) Effects of DL-malate on in vitro forage fiber digestion by mixed ruminal microorganisms. Curr Microbiol 48, 2731.
Martin, SA & Streeter, MN (1995) Effect of malate on in vitro mixed ruminal microorganism fermentation. J Anim Sci 73, 21412145.
Newbold, CJ, Wallace, RJ & McIntosh, FM (1996) Mode of action of the yeast Saccharomyces cerevisiae as a feed additive for ruminants. Br J Nutr 76, 249261.
Nisbet, DJ & Martin, SA (1990) Effect of dicarboxylic acids and Aspergillus oryzae fermentation extract on lactate uptake by the ruminal bacterium Selenomonas ruminantium. Appl Environ Microb 56, 35153518.
Nisbet, DJ & Martin, SA (1993) Effects of fumarate, L-malate, and an Aspergillus oryzae fermentation extract on D-lactate utilization by the ruminal bacterium Selenomonas ruminantium. Curr Microbiol 26, 133136.
Ranilla, MJ & Carro, MD (2003) Diet and procedures to detach particle-associated microbes from ruminal digesta influence chemical composition of microbes and estimation of microbial growth in Rusitec fermenters. J Anim Sci 81, 537544.
Russell, JB (1998) The importance of pH in the regulation of ruminal acetate to propionate ratio and methane production in vitro. J Dairy Sci 81, 32223230.
Russell, JB, Van Soest, PJ (1984) In vitro ruminal fermentation of organic acids common in forage. Appl Environ Microb 47, 155159.
Stewart, CS (1977) Factors affecting cellulolytic activity of rumen contents. Appl Environ Microb 33, 497502.
Tejido, ML, Ranilla, MJ, García-Martinez, R & Carro, MD (2005) In vitro microbial growth and rumen fermentation of diets differing their forage:concentrate ratio as affected by the addition of disodium malate Anim Sci (In the press)
Van Soest, PJ, Robertson, JB & Lewis, BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 74, 35833597.
Weatherburn, MW (1967) Phenol-hypochlorite reaction for determination of ammonia. Anal Chem 39, 971974.

Keywords

Influence of disodium malate on microbial growth and fermentation in rumen-simulation technique fermenters receiving medium- and high-concentrate diets

  • J. A. Gómez (a1), M. L. Tejido (a1) and M. D. Carro (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.