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Influence of different concentrations of disodium fumarate on methane production and fermentation of concentrate feeds by rumen micro-organisms in vitro

  • M. D. Carro (a1) and M. J. Ranilla (a1)

Abstract

Batch cultures of mixed rumen micro-organisms were used to study the effects of different concentrations of disodium fumarate on the fermentation of five concentrate feeds (maize, barley, wheat, sorghum and cassava meal). Rumen contents were collected from four Merino sheep fed lucerne hay ad libitum and supplemented with 300 g concentrate/d. Disodium fumarate was added to the incubation bottles to achieve final concentrations of 0, 4, 7 and 10 mm-fumarate. In 17 h incubations, the final pH and total volatile fatty acid production increased (P<0·001) linearly for all substrates as fumarate concentration increased from 0 to 10 mm. Propionate and acetate production increased (P<0·05), while the value of the acetate:propionate ratio decreased (P<0·05) linearly with increasing doses of fumarate. In contrast, l-lactate and NH3-N concentrations in the cultures were not affected (P>0·05) by the addition of fumarate. For all substrates, fumarate treatment decreased (P<0·05) CH4 production, the mean values of the decrease being 2·3, 3·8 and 4·8 % for concentrations of 4, 7 and 10 mm-fumarate respectively. Addition of fumarate did not affect (P>0·05) the total gas production. If the results of the present experiment are confirmed in vivo, fumarate could be used as a feed additive for ruminant animals fed high proportions of cereal grains, because it increased pH, acetate and propionate production and it decreased CH4 production.

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Corresponding author

*Corresponding author:Dr M. D. Carro, fax +34 987 291311, email DP1MCT@UNILEON.ES

References

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Asanuma, N, Iwamoto, M & Hino, T (1999) Effect of the addition of fumarate on methane production by ruminal microorganisms in vitro. J Dairy Sci 82, 780787.
Association of Official Analytical Chemists (1995) Official Methods of Analysis, 16th ed., chapter 4, p. 13. Arlington, VA: AOAC.
Bayaru, E, Syuhei, K & Toshihiko, K, et al. (2001) Effect of fumaric acid on methane production, rumen fermentation and digestibility of cattle fed roughage alone. Anim Sci J 72, 139146.
Caldwell, DR & Bryant, MP (1966) Medium without rumen fluid for non-selective enumeration and isolation of rumen bacteria. Appl Microbiol 14, 794801.
Callaway, TR & Martin, SA (1996) Effects of organic acid and monensin treatment on in vitro mixed ruminal microorganism fermentation of cracked corn. J Anim Sci 74, 19821989.
Carro, MD, López, S, Valdés, C & Ovejero, FJ (1999) Effect of dl-malate on mixed ruminal microorganism fermentation using the rumen simulation technique (RUSITEC). Anim Feed Sci Technol 79, 279288.
Carro, MD & Ranilla, MJ (2003) Effect of the addition of malate on in vitro rumen fermentation of cereal grains. Br J Nutr 89, 181188.
Carro, MD, Valdés, C, Ranilla, MJ & González, JS (2000) Effect of forage to concentrate ratio in the diet on ruminal fermentation and digesta flow kinetics in sheep. Anim Sci 70, 127134.
Demeyer, DI & Henderickx, MK (1967) Competitive inhibition of in vitro methane production by mixed rumen bacteria. Arch Int Physiol Biochim 75, 157159.
Demeyer, DI & Fievez, V (2000) Ruminants et environnement: la méthanogenèse (Ruminants and environment: methanogenesis). Ann Zootech 49, 95112.
Goering, MK & Van Soest, PJ (1970) Forage Fiber Analysis (Apparatus, Reagents, Procedures and Some Applications) Agricultural Handbook, no. 379. Washington, DC: ARS, USDA.
López, S, Valdés, C, Newbold, CJ & Wallace, RJ (1999) Influence of sodium fumarate addition on rumen fermentation in vitro. Br J Nutr 81, 5964.
Martin, SA (1998) Manipulation of ruminal fermentation with organic acids: a review. J Anim Sci 76, 31233132.
Mould, FL (1988) Associative effects of feeds. In Feed Science World. Animal Science, B4, pp. 279292 [Ørskov, ER, editor]. Amsterdam: Elsevier Science Publishers BV.
Newbold, CJ, Wallace, RJ & McIntosh, FM (1996) Mode of action of the yeast Saccharomyces cerevisiae as a feed additive for ruminants. Br J Nutr 76, 249261.
Nisbet, DJ & Martin, SA (1990) Effect of dicarboxylic acids and Aspergillus oryzae fermentation extract on lactate uptake by the ruminal bacterium Selenomonas ruminantium. Appl Environ Microbiol 26, 133136.
Nisbet, DJ & Martin, SA (1993) Effects of fumarate, l-malate, and an Aspergillus oryzae fermentation extract on d-lactate utilization by the ruminal bacterium Selenomonas ruminantium. Curr Microbiol 26, 133136.
Russell, JB & Wallace, RJ (1997) Energy consuming and yielding mechanisms. In The Rumen Microbial Ecosystem, 2nd ed. pp. 246282 [Hobson, PN and Stewart, CS, editors]. London: Chapman & Hall.
Van Kessel, JAS & Russell, JB (1996) The effect of pH on ruminal methanogenesis. FEMS Microbiol Ecol 20, 205210.
Van Soest, PJ, Robertson, JB & Lewis, BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 74, 35833597.
Weatherburn, MW (1967) Phenol-hypochlorite reaction for determination of ammonia. Anal Chem 39, 971974.

Keywords

Influence of different concentrations of disodium fumarate on methane production and fermentation of concentrate feeds by rumen micro-organisms in vitro

  • M. D. Carro (a1) and M. J. Ranilla (a1)

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