Hostname: page-component-77c89778f8-m8s7h Total loading time: 0 Render date: 2024-07-25T01:34:12.671Z Has data issue: false hasContentIssue false
  • English
  • Français

In vivo and in vitro methods of measuring nutritive value of leaf-protein preparations

Published online by Cambridge University Press:  09 March 2007

R. A. Buchanan
R. A. Buchanan
Affiliation:
Rothamsted Experimental Station, Harpenden, Herts.
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Several in vitro methods were compared with in vivo methods for estimating the nutritive value of leaf-protein concentrates (LPC), using a freeze-dried preparation from wheat (A) fresh, (B) after heating moist, (C) after heating moist and then extraction with chloroform, and (D) after extraction with an acidified solvent.

2. The treatments had little effect on the biological value (BV) of the samples for rats.

3. Heating moist decreased true digestibility (TD), net protein utilization (NPU) and protein efficiency ratio (PER), but the original values were almost restored by lipid extraction. Acidified solvent extraction decreased TD, NPU and PER of LPC perhaps by making it brittle and difficult to wet.

4. Papain solubility and TD were well correlated. Pepsin-pancreatin solubility and TD were less well correlated.

5. Microbiological estimations of available amino acids, involving predigestion with pepsin, correlated poorly with TD determinations.

6. Unsaturated fatty acids, particularly linolenic, formed complexes during heating of LPC. The effect of this on enzyme solubilization procedures and on digestion in vivo is discussed.

7. Some comparisons are made between the effect of heat and of extraction with solvents on LPC and on fish meal.

Type
Research Article
Information
British Journal of Nutrition , Volume 23 , Issue 3 , August 1969 , pp. 533 - 545
Copyright
Copyright © The Nutrition Society 1969

References

Akeson, W. R. & Stahmann, M. A. (1964). J. Nutr. 83, 257.CrossRefGoogle Scholar
Akeson, W. R. & Stahmann, M. A. (1965). J. agric. Fd Chem. 13, 145.CrossRefGoogle Scholar
Buchanan, R. A. (1968). Changes in leaf protein preparations during storage. PhD Thesis, London University.Google Scholar
Buchanan, R. A. (1969). J. Sci. Fd Agric. (In the Press.)Google Scholar
Buchanan, R. A. & Byers, M. (1969). J. Sci. Fd Agric. (In the Press.)Google Scholar
Carpenter, K. J., Lea, C. H. & Parr, L. J. (1963). Br. J. Nutr. 17, 151.CrossRefGoogle Scholar
Duckworth, J., Hepburn, W. R. & Woodham, A. A. (1961). J. Sci. Fd Agric. 12, 16.CrossRefGoogle Scholar
Duckworth, J. & Woodham, A. A. (1961). J. Sci. Fd Agric. 12, 5.CrossRefGoogle Scholar
Ellinger, G. M. (1954). Wld's Poult. Congr. X. Edinburgh Part 2, p. 128.Google Scholar
FAO (1957). F.A.O. nutr. Stud. no. 16.Google Scholar
Folch, J., Lees, M. & Stanley, G. H. S. (1957). J. biol. Chem. 226, 497.CrossRefGoogle Scholar
Ford, J. E. (1962). Br. J. Nutr. 16, 409.CrossRefGoogle Scholar
Ford, J. E. & Salter, D. N. (1966). Br. J. Nutr. 20, 843.CrossRefGoogle Scholar
Geisler, H. W. & Contreras, E. (1967). Fshg News int. 6, 38.Google Scholar
Gerloff, E. D., Lima, I. H. & Stahmann, M. A. (1965). J. agric. Fd Chem. 13, 139.CrossRefGoogle Scholar
Henry, K. M. (1959). Rep. natn. Inst. Res. Dairy. p. 97.Google Scholar
Henry, K. M. & Ford, J. E. (1965). J. Sci. Fd Agric. 16, 425.CrossRefGoogle Scholar
Henry, K. M., Kon, S. K. & Watson, M. B. (1937). In Milk and Nutrition, Part I, p. 37. Reading: National Institute for Research in Dairying.Google Scholar
Kaplan, A. (1965). Stand. Meth. clin. Chem. 5, 245.CrossRefGoogle Scholar
Lima, I. H., Richardson, T. & Stahmann, M. A. (1965). J. agric. Fd Chem. 13, 143.CrossRefGoogle Scholar
Mitchell, H. H. (1924). J. biol. Chem. 58, 873.CrossRefGoogle Scholar
Mitchell, H. H. & Carman, G. G. (1926). J. biol. Chem. 68, 183.CrossRefGoogle Scholar
Morrison, A. B., Sabry, Z. I. & Middleton, E. J. (1962). J. Nutr. 77, 97.CrossRefGoogle Scholar
Morrison, J. E. & Pirie, N. W. (1961). J. Sci. Fd Agric. 12, 1.CrossRefGoogle Scholar
Nichols, B. W., Harris, R. V. & James, A. T. (1965). Biochem. biophys. Res. Commun. 20, 256.CrossRefGoogle Scholar
Olley, J. & Pirie, R. (1966). Fshg News int. 5, (12), 27.Google Scholar
Pleshkov, B. P. & Fowden, L. (1959). Nature, Lond. 183, 1445.CrossRefGoogle Scholar
Stoffel, W., Insull, W. Jr & Ahrens, E. H. Jr (1958). Proc. Soc. exp. Biol. Med. 99, 238.CrossRefGoogle Scholar
Stott, J. A. & Smith, H. (1966). Br. J. Nutr. 20, 663.CrossRefGoogle Scholar
Stott, J. A., Smith, H. & Rosen, G. D. (1963). Br. J. Nutr. 19, 227.CrossRefGoogle Scholar
Valli Devi, A., Rao, N. A. N. & Vijayaraghavan, P. K. (1965). J. Sci. Fd Agric 16, 116.CrossRefGoogle Scholar
Waterlow, J. C. (1962). Br. J. Nutr. 16, 531.CrossRefGoogle Scholar