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Improvement of calcium balance by Fructus Ligustri Lucidi extract in mature female rats was associated with the induction of serum parathyroid hormone levels

  • Xiao-Li Dong (a1) (a2), Ming Zhao (a3), Kwun-Kit Wong (a1), Chun-Tao Che (a3) (a4) and Man-Sau Wong (a1) (a5)...

Abstract

Fructus Ligustri Lucidi (FLL) is a commonly prescribed herb in many kidney-tonifying Traditional Chinese Medicinal formulae for the treatment of osteoporosis. The present study aimed to identify the active fractions in FLL and to characterise its effects on Ca balance, calciotropic hormone levels as well as bone properties in mature female rats fed diets containing different levels of Ca. In the present study, 4-month-old Sprague–Dawley female rats were treated with either FLL ethanol extract (EE), ethyl acetate-soluble fraction of EE (EAF), water-soluble fraction of EE (WF) or their vehicle for 12 weeks on a medium-Ca diet (MCD, 0·6 % Ca, 0·65 % P). Then, the Sprague–Dawley female rats treated with WF or its vehicle for 12 weeks were fed diets containing different levels of dietary Ca (low-Ca diet (LCD), 0·1 % Ca, 0·65 % P; MCD; high-Ca diet (HCD), 1·2 % Ca, 0·65 % P). The results demonstrated that WF from EE but not EAF exerted a prominent effect on Ca balance by inhibiting urinary and faecal Ca excretion. WF significantly increased Ca balance in rats fed MCD or HCD with an associated increase in serum parathyroid hormone (PTH) levels. WF did not alter bone mineral density or bone mineral content of the tibia in all the rats fed with different levels of dietary Ca. In conclusion, WF was responsible for the positive actions of FLL on Ca absorption and balance. The regulation of Ca balance by WF might involve its action in stimulating PTH production in the mature female rats.

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Copyright

Corresponding author

*Corresponding author: Dr M.-S. Wong, fax +852 23649932, email bcmswong@polyu.edu.hk

References

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1Neer, RM, Arnaud, CD, Zanchetta, JR, et al. (2001) Effect of parathyroid hormone (1–34) on fractures and bone mineral density in postmenopausal women with osteoporosis. N Engl J Med 344, 14341441.
2Zhang, Y, Lai, WP, Leung, PC, et al. (2008) Improvement of Ca balance by Fructus Ligustri Lucidi extract in aged female rats. Osteoporos Int 19, 235242.
3Zhang, Y, Leung, PC, Che, CT, et al. (2008) Improvement of bone properties and enhancement of mineralization by ethanol extract of Fructus Ligustri Lucidi. Br J Nutr 99, 494502.
4Dong, XL, Zhang, Y, Favus, MJ, et al. (2010) Ethanol extract of Fructus Ligustri Lucidi increases circulating 1,25-dihydroxyvitamin D3 by inducing renal 25-hydroxyvitamin D-1alpha hydroxylase activity. Menopause 17, 11741181.
5Ward, DT, Maldonado-Perez, D, Hollins, L, et al. (2005) Aminoglycosides induce acute cell signaling and chronic cell death in renal cells that express the calcium-sensing receptor. J Am Soc Nephrol 16, 12361244.
6Hoenderop, JG, Nilius, B & Bindels, RJ (2005) Calcium absorption across epithelia. Physiol Rev 85, 373422.
7Tudpor, K, Teerapornpuntakit, J, Jantarajit, W, et al. (2008) 1,25-dihydroxyvitamin D(3) rapidly stimulates the solvent drag-induced paracellular calcium transport in the duodenum of female rats. J Physiol Sci 58, 297307.
8Fujita, H, Sugimoto, K, Inatomi, S, et al. (2008) Tight junction proteins claudin-2 and -12 are critical for vitamin D-dependent Ca2+ absorption between enterocytes. Mol Biol Cell 19, 19121921.
9Yim, TK, Wu, WK, Pak, WF, et al. (2001) Hepatoprotective action of an oleanolic acid-enriched extract of Ligustrum lucidum fruits is mediated through an enhancement on hepatic glutathione regeneration capacity in mice. Phytother Res 15, 589592.
10Shi, L, Cai, Z, Wu, G, et al. (1998) RP-HPLC determination of water-soluble active constituents and oleanolic acid in the fruits of Ligustrum lucidum Ait. collected from various areas. Zhongguo Zhong Yao Za Zhi 23, 7779, 127.
11Li, G, Zhang, XA, Zhang, JF, et al. (2010) Ethanol extract of Fructus Ligustri Lucidi promotes osteogenesis of mesenchymal stem cells. Phytother Res 24, 571576.
12Qiu, RL & Li, L (2008) Study on extraction methods of polysaccharides in Fructus Ligustri Lucidi. Chin Traditional Patent Med 30, 612614.
13Zhang, ZM, Ge, B, Xu, AX, et al. (2006) Antisenile effect of polysaccharides from Fructus ligustri lucidi. Chin J Pharmacol Toxicol 20, 108111.
14Ruan, H & Lu, Z (1999) Studies on immunomodulatory function of polysaccharide of Fructus Ligustri Lucidi. Zhongguo Zhong Yao Za Zhi 24, 691693, 704.
15Council, NR (2006) Guide for the Care and the Use of Laboratory Animals. Washington, DC: National Academy Press.
16Lai, WP, Chau, TS, Cheung, PY, et al. (2003) Adaptive responses of 25-hydroxyvitamin D3 1-alpha hydroxylase expression to dietary phosphate restriction in young and adult rats. Biochim Biophys Acta 1639, 3442.
17Armbrecht, HJ, Boltz, MA & Bruns, ME (2003) Effect of age and dietary calcium on intestinal calbindin D-9k expression in the rat. Arch Biochem Biophys 420, 194200.
18Dusso, AS, Brown, AJ & Slatopolsky, E (2005) Vitamin D. Am J Physiol Renal Physiol 289, F8F28.
19Carrillo-Lopez, N, Fernandez-Martin, JL & Cannata-Andia, JB (2009) The role of calcium, calcitriol and their receptors in parathyroid regulation. Nefrologia 29, 103108.
20Silver, J, Kilav, R & Naveh-Many, T (2002) Mechanisms of secondary hyperparathyroidism. Am J Physiol Renal Physiol 283, F367F376.
21Goodman, WG (2004) The flavors of vitamin D: tasting the molecular mechanisms. Kidney Int 66, 12861287.
22Zhang, Y, Lai, WP, Wu, CF, et al. (2007) Ovariectomy worsens secondary hyperparathyroidism in mature rats during low-Ca diet. Am J Physiol Endocrinol Metab 292, E723E731.
23Zhang, Y, Dong, XL, Leung, PC, et al. (2008) Fructus ligustri lucidi extract improves calcium balance and modulates the calciotropic hormone level and vitamin D-dependent gene expression in aged ovariectomized rats. Menopause 15, 558565.
24Qin, L, Raggatt, LJ & Partridge, NC (2004) Parathyroid hormone: a double-edged sword for bone metabolism. Trends Endocrinol Metab 15, 6065.
25Greenspan, SL, Bone, HG, Ettinger, MP, et al. (2007) Effect of recombinant human parathyroid hormone (1–84) on vertebral fracture and bone mineral density in postmenopausal women with osteoporosis: a randomized trial. Ann Intern Med 146, 326339.
26Ettinger, B, San Martin, J, Crans, G, et al. (2004) Differential effects of teriparatide on BMD after treatment with raloxifene or alendronate. J Bone Miner Res 19, 745751.
27John, MR, Widler, L, Gamse, R, et al. (2011) ATF936, a novel oral calcilytic, increases bone mineral density in rats and transiently releases parathyroid hormone in humans. Bone 49, 233241.
28Kumar, S, Matheny, CJ, Hoffman, SJ, et al. (2009) An orally active calcium-sensing receptor antagonist that transiently increases plasma concentrations of PTH and stimulates bone formation. Bone 46, 534542.
29Brommage, R, Binacua, C, Antille, S, et al. (1993) Intestinal calcium absorption in rats is stimulated by dietary lactulose and other resistant sugars. J Nutr 123, 21862194.
30Mitamura, R, Hara, H & Aoyama, Y (2004) Ingestion of raffinose promotes calcium absorption in the large intestine of rats. Biosci Biotechnol Biochem 68, 384389.
31Griessen, M, Speich, PV, Infante, F, et al. (1989) Effect of absorbable and nonabsorbable sugars on intestinal calcium absorption in humans. Gastroenterology 96, 769775.
32Lacour, B, Ohan, J, Aznag, A, et al. (1995) Stimulating effects of sorbitol and l-xylose on rat ileal Ca transport in vitro. Miner Electrolyte Metab 21, 391397.
33Tardivel, S, Fournier, P, Dupuis, Y, et al. (1994) Stimulation of ileal calcium absorption by sorbitol, l-xylose, or creatine via a decrease in luminal sodium concentration: relation with concomitant changes in enterocyte energy metabolism. Calcif Tissue Int 54, 304311.
34Takahara, S, Morohashi, T, Sano, T, et al. (2000) Fructooligosaccharide consumption enhances femoral bone volume and mineral concentrations in rats. J Nutr 130, 17921795.
35Morohashi, T, Sano, T, Ohta, A, et al. (1998) True calcium absorption in the intestine is enhanced by fructooligosaccharide feeding in rats. J Nutr 128, 18151818.
36Mineo, H, Hara, H, Kikuchi, H, et al. (2001) Various indigestible saccharides enhance net calcium transport from the epithelium of the small and large intestine of rats in vitro. J Nutr 131, 32433246.
37van den Heuvel, EG, Schoterman, MH & Muijs, T (2000) Transgalactooligosaccharides stimulate calcium absorption in postmenopausal women. J Nutr 130, 29382942.
38Griffin, IJ, Davila, PM & Abrams, SA (2002) Non-digestible oligosaccharides and calcium absorption in girls with adequate calcium intakes. Br J Nutr 87, Suppl. 2, S187S191.
39Scholz-Ahrens, KE, Schaafsma, G, van den Heuvel, EG, et al. (2001) Effects of prebiotics on mineral metabolism. Am J Clin Nutr 73, 459S464S.
40Suzuki, T & Hara, H (2004) Various nondigestible saccharides open a paracellular calcium transport pathway with the induction of intracellular calcium signaling in human intestinal Caco-2 cells. J Nutr 134, 19351941.
41Takasaki, M, Inaba, H, Ohta, A, et al. (2000) Dietary short-chain fructooligosaccharides increase calbindin-D9k levels only in the large intestine in rats independent of dietary calcium deficiency or serum 1,25 dihydroxy vitamin D levels. Int J Vitam Nutr Res 70, 206213.

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