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Home use of margarine is an important determinant of plasma trans fatty acid status: a biomarker study

  • C. Murray Skeaff (a1) and Sonya Gowans (a1)


The contribution of the home use of margarines, made with partially hydrogenated vegetables oils, to total trans fatty acid intake is difficult to determine using dietary assessment because food composition databases are incomplete for trans fatty acids; moreover, hidden fats in manufactured foods may be the predominant sources of trans fatty acids. The objective of our study was to determine, using plasma phospholipid trans fatty acid composition as a surrogate measure of exposure, whether the home use of margarine or butter is an important determinant of trans fatty acid status. We conducted a community-based (Dunedin, New Zealand), cross-sectional survey of people who consumed either margarine (n 65) or butter (n 64) but not both for home use. The levels of the 18:1 trans isomers commonly found in partially hydrogenated vegetable oils were all significantly higher in the plasma phospholipids of margarine compared with butter consumers, with the exception of 18:1n-7t, which did not differ. Among margarine consumers, the percentage of total fat from margarine was significantly correlated with levels of phospholipid 18:1n−6t, 18:1n-8t and 18:1n-1/t isomers (r 0·57–0·63, P<0·001) but only weakly with 18:1n-7t (r 0·30, P=0·016). The intake of fat from fast foods, bakery products or meat and meat products was not associated with plasma phospholipid trans isomeric composition. The home use of margarine, made with partially hydrogenated vegetable oils, is an important determinant of trans fatty acid exposure in New Zealand.

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Corresponding author

*Corresponding author: Dr C. Murray Skeaff, fax +64 3 479 7958, email


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Ascherio, A, Rimm, EB, Giovannucci, EL, Spiegelman, D, Stampfer, M, Willett, WCDietary fat and risk of coronary heart disease in men: cohort follow up study in the United States. BMJ (1996) 313, 8490.
Ball, MJ, Hackett, D, Duncan, ATrans fatty acid content of margarines, oils and blended spreads available in New Zealand. Asia Pacif J Clin Nutr (1993) 2, 165169.
Beaton, GH, Milner, J, Corey, P et al. Sources of variance in 24-hour dietary recall data: implications for nutrition study design and interpretation. Am J Clin Nutr (1979) 32, 25462559.
Bingham, SThe dietary assessment of individuals; methods, accuracy, new techniques and recommendations. Nutr Abstr Rev (1987) 57 705742.
Bingham, SA, Gill, C, Welch, A, Day, K, Cassidy, A, Khaw, KT, Sneyd, MJ, Key, TJ, Roe, L, Day, NEComparison of dietary assessment methods in nutritional epidemiology: weighed records v. 24 h recalls, food-frequency questionnaires and estimated-diet records. Br J Nutr (1994) 72, 619643.
Burlingame, B, Milligan, G, Apimerika, D, Arthur, JThe New Zealand Food Composition Tables Palmerston North, New ZealandNew Zealand Institute for Crop and Food Research (1993)
Chisholm, A, Mann, J, Sutherland, W, Duncan, A, Skeaff, M, Frampton, CEffect on lipoprotein profile of replacing butter with margarine in a low fat diet: randomised crossover study with hypercholesterolaemic subjects. BMJ (1996) 312, 931934.
Elias, SL, Innis, SMBakery foods are the major dietary source of trans-fatty acids among pregnant women with diets providing 30 percent energy from fat. J Am Diet Assoc (2002) 102, 4651.
Emken, EA, Rohwedder, WK, Adlof, RO, DeJarlais, WJ, Gulley, RMAbsorption and distribution of deuterium-labeled trans- and cis-11-octadecenoic acid in human plasma and lipoprotein lipids. Lipids (1986) 21, 589595.
Firestone, D, Sheppard, ADetermination of trans fatty acids Advances in Lipid Methodology Christie, WABridgwater, UKOily Press (1992)
Goldberg, GR, Black, AE, Jebb, SA, Cole, TJ, Murgatroyd, PR, Coward, WA, Prentice, AMCritical evaluation of energy intake data using fundamental principles of energy physiology. 1. Derivation of cut-off limits to identify under-recording. Eur J Clin Nutr (1991) 45, 569581.
Holub, BJ, Skeaff, CMNutritional regulation of cellular phosphatidylinositol. Methods Enzymol (1987) 141, 234244.
Hu, FB, Stampfer, MJ, Manson, JE, Rimm, E, Colditz, GA, Rosner, BA, Hennekens, CH, Willett, WCDietary fat intake and the risk of coronary heart disease in women. N Eng J Med (1997) 337, 14911499.
Hudgins, LC, Hirsch, J, Emken, EACorrelation of isomeric fatty acids in human adipose tissue with clinical risk factors for cardiovascular disease. Am J Clin Nutr (1991) 53, 474482.
Innis, SM, Green, TJ, Halsey, TKVariability in the trans fatty acid content of foods within a food category: implications for estimation of dietary trans fatty acid intakes. J Am Coll Nutr (1999) 18, 255260.
Jensen, RGThe composition of bovine milk lipids: January 1995 to December 2000. J Dairy Sci (2002) 85, 295350.
Lake, R, Thomson, B, Devane, G, Scholes, PTrans fatty acid content of selected New Zealand foods. J Food Compost Anal (1996) 9, 365374.
Lichtenstein, AH, Ausman, LM, Jalbert, SM, Schaefer, EJEffects of different forms of dietary hydrogenated fats on serum lipoprotein cholesterol levels. N Engl J Med (1999) 340, 19331940.
Lichtenstein, AH, Erkkila, AT, Lamarche, B, Schwab, US, Jalbert, SM, Ausman, LMInfluence of hydrogenated fat and butter on CVD risk factors: remnant-like particles, glucose and insulin, blood pressure and C-reactive protein. Atherosclerosis (2003) 171, 97107.
Mahfouz, MM, Valicenti, AJ, Holman, RTDesaturation of isomeric trans-octadecenoic acids by rat liver microsomes. Biochim Biophys Acta (1980) 618, 112.
Marchand, CPositional isomers of trans-octadecenoic acids in margarines. Can Inst Food Sci Technol J (1982) 15, 196199.
Mensink, RP, Katan, MBEffect of dietary trans fatty acids on high-density and low-density lipoprotein cholesterol levels in healthy subjects. N Eng J Med (1990) 323, 439445.
Molkentin, J, Precht, DIsomeric distribution and rapid determination of trans-octadecenoic acids in German brands of partially hydrogenated edible fats. Nahrung (1996) 6, 297304.
Parodi, PWDistribution of isomeric octadecenoic fatty acids in milk fat. J Dairy Sci (1976) 59, 18701873.
Pietinen, P, Ascherio, A, Korhonen, P, Hartman, AM, Willett, WC, Albanes, D, Virtamo, JIntake of fatty acids and risk of coronary heart disease in a cohort of Finnish men. The Alpha-Tocopherol, Beta-Carotene Cancer Prevention Study. Am J Epidemiol (1997) 145, 876887.
Pollard, MR, Gunstone, FD, James, AT, Morris, LJDesaturation of positional and geometric isomers of monoenoic fatty acids by microsomal preparations from rat liver. Lipids (1980) 15, 306314.
Precht, D, Molkentin, JTrans fatty acids: implications for health, analytical methods, incidence in edible fats and intake (a review). Nahrung (1995) 39, 343374.
Ratnayake, W, Beare-Rogers, JProblems of analysing C18 cis- and trans-fatty acids of margarine on the SP-2340 capillary column. J Chromatogr Sci (1990) 28, 633639.
Ratnayake, W, Pelletier, GPositional and geometrical isomers of linoleic acid in partially hydrogenated oils. J Am Oil Chem Soc (1992) 69, 95105.
Seppanen-Laakso, T, Laakso, I, Backlund, P, Vanhanen, H, Viikari, JElaidic and trans-vaccenic acids in plasma phospholipids as indicators of dietary intake of 18:1 trans-fatty acids. J Chromatogr B Biomed Appl (1996) 687, 371378.
Skeaff, CM, Mann, JI, McKenzie, J, Wilson, NC, Russell, DGDeclining levels of total serum cholesterol in adult New Zealanders. N Z Med J (2001) 114, 131134.
Slover, HT, Thompson, RHJ, Davis, CS, Merola, GVLipids in margarines and margarine-like foods. J Am Oil Chem Soc (1985) 62, 775786.
Smedman, AE, Gustafsson, IB, Berglund, LG, Vessby, BOPentadecanoic acid in serum as a marker for intake of milk fat: relations between intake of milk fat and metabolic risk factors. Am J Clin Nutr (1999) 69, 2229.
van de Vijver, LP, van Poppel, G, van Houwelingen, A, Kruyssen, DA, Hornstra, GTrans unsaturated fatty acids in plasma phospholipids and coronary heart disease: a case-control study. Atherosclerosis (1996) 126, 155161.
Vidgren, HM, Louheranta, AM, Agren, JJ, Schwab, US, Uusitupa, MIDivergent incorporation of dietary trans fatty acids in different serum lipid fractions. Lipids (1998) 33, 955962.
Wolff, RContent and distribution of trans-18:1 acids in ruminant milk and meat fats. Their importance in European diets and their effect on human milk. J Am Oil Chem Soc (1995) 72, 259272.



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