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Glucose and ]13C[leucine metabolism by the portal-drained viscera of sheep fed on dried grass with acute intravenous and intraduodenal infusions of glucose

Published online by Cambridge University Press:  09 March 2007

F. Piccioli Cappelli ,
C. J. Seal  and
D. S. Parker
F. Piccioli Cappelli
Affiliation:
Department of Biological and Nutritional Sciences, University of Newcastle, Newcastle upon Tyne NE1 7RU
C. J. Seal
Affiliation:
Department of Biological and Nutritional Sciences, University of Newcastle, Newcastle upon Tyne NE1 7RU
D. S. Parker
Affiliation:
Department of Biological and Nutritional Sciences, University of Newcastle, Newcastle upon Tyne NE1 7RU
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Abstract

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The effect of exogenous glucose supply by either intrajugular (IJG) or intraduodenal (IDG) infusion at 2.0 mg glucose/kg body weight per min was investigated in four wether sheep (average weight 44 (sd 4) kg) chronically catheterized in the carotid artery and portal veins. Sheep were fed on a dried grass pellet diet hourly using continuous belt feeders. Whole-body glucose irreversible loss (IL) rate, measured with [6-3H[glucose, was increased by 0.5 and 0.8 of exogenous supply for IJG and IDG infusions respectively. Portal glucose utilization, measured by isotope dilution across the portal-drained viscera, was unaffected by additional glucose regardless of the route of glucose supply (P = 0.76 for control ν. glucose infusions) and was a constant proportion of glucose IL (0.28) for all treatments. Portal plasma flow was higher during IDG infusions compared with IJG infusions (1.65 ν. 1.44 litres/min, P=0.055). Circulating total free amino acid concentrations fell during glucose infusions (2146, 1808 and 1683 μmol/l for control, IJG and IDG treatments respectively, P=0.067 for treatment effect) but net portal absorption was not affected by increased glucose supply. Recovery in the portal vein of [1-13C[leucine infused into the duodenum averaged 0.65 and was not affected by increasing glucose supply to the gut tissues. The results show that glucose utilization by gut tissues is responsive to changes in both vascular and luminal glucose supply. The effects of changing gut tissue use of glucose and increased whole body glucose IL on metabolism of nutrients is discussed.

Keywords

Glucose metabolismLeucine absorptionGut tissue metabolismSheep
Type
Animal Nutrition
Information
British Journal of Nutrition , Volume 78 , Issue 6 , December 1997 , pp. 931 - 946
Copyright
Copyright © The Nutrition Society 1997

References

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