Skip to main content Accessibility help
×
Home

Effects of dietary protein content and 2-hydroxy-4-methylthiobutanoic acid or dl-methionine supplementation on performance and oxidative status of broiler chickens

  • Quirine Swennen (a1), Pierre-André Geraert (a2), Yves Mercier (a2), Nadia Everaert (a1), Anneleen Stinckens (a1), Hilke Willemsen (a1), Yue Li (a1), Eddy Decuypere (a1) and Johan Buyse (a1)...

Abstract

Besides its typical role as an amino acid in protein synthesis, methionine is an important intermediate in methylation reactions. In addition, it can also be converted to cysteine and hence plays a role in the defence against oxidative stress. The present study was conducted to investigate further the role of dl-methionine (DLM) and its hydroxy analogue, dl-2-hydroxy-4-methylthiobutanoic acid (DL-HMTBA), on zootechnical performance and oxidative status of broiler chickens. Male broiler chickens were reared on two diets differing in crude protein (CP) content (low-protein, 18·3 % v. high-protein, 23·2 % CP) and were supplemented either with 0·25 % DLM or 0·25 % DL-HMTBA. Reducing the dietary protein content resulted in an impaired body weight gain (P < 0·0001). However, supplementation of DL-HMTBA to the low-protein diet partially alleviated these negative effects (P = 0·0003). This latter phenomenon could be explained by the fact that chickens fed DL-HMTBA-supplemented diets displayed a better antioxidant status as reflected in lower lipid peroxidation probably as a consequence of their higher hepatic concentrations of total and reduced glutathione compared with their DLM counterparts. On the other hand, within the high protein levels, uric acid might be an important antioxidant to explain the lower lipid peroxidation of high-protein DL-HMTBA-supplemented chickens. Hepatic methionine sulfoxide reductase-A gene expression was not significantly affected by the dietary treatments. In conclusion, the present study indicates that there are interactions between dietary protein content and supplementation of methionine analogues with respect to broiler performance and antioxidant status, also suggesting a causal link between these traits.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Effects of dietary protein content and 2-hydroxy-4-methylthiobutanoic acid or dl-methionine supplementation on performance and oxidative status of broiler chickens
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Effects of dietary protein content and 2-hydroxy-4-methylthiobutanoic acid or dl-methionine supplementation on performance and oxidative status of broiler chickens
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Effects of dietary protein content and 2-hydroxy-4-methylthiobutanoic acid or dl-methionine supplementation on performance and oxidative status of broiler chickens
      Available formats
      ×

Copyright

Corresponding author

*Corresponding author: Professor J. Buyse, fax +32 16 321994, email johan.buyse@biw.kuleuven.be

References

Hide All
1 Sauer, N, Emrich, K, Piepho, H-P, et al. (2008) Meta-analysis of the relative efficiency of methionine-hydroxy-analogue-free-acid compared with dl-methionine in broilers using nonlinear mixed models. Poult Sci 87, 20232031.
2 Zhang, LB & Guo, YM (2008) Effects of liquid dl-2-hydroxy-4-methylthio buanoic acid on growth performance and immune responses in broiler chickens. Poult Sci 87, 13701376.
3 Garcia Neto, M, Pesti, GM & Bakalli, RI (2000) Influence of dietary protein level on the broiler chicken's response to methionine and betaine supplements. Poult Sci 79, 14781484.
4 Takahashi, K, Konashi, S & Akiba, Y (1994) The effects of dietary methionine and dispensable amino acid supplementation on abdominal fat deposition in male broilers. Anim Sci Tech 65, 244250.
5 Vieira, SL, Lemme, A, Goldenberg, DB, et al. (2004) Responses of growing broilers to diets with increased sulfur amino acids to lysine ratios at two dietary protein levels. Poult Sci 83, 13071313.
6 Fatufe, AA & Rodehutscord, M (2005) Growth, body composition, and marginal efficiency of methionine utilization are effected by nonessential amino acid nitrogen supplementation in male broiler chicken. Poult Sci 84, 15841592.
7 Métayer, S, Seiliez, I, Collin, A, et al. (2008) Mechanisms through which sulfur amino acids control protein metabolism and oxidative status. J Nutr Biochem 19, 207215.
8 Stadtman, ER, Van Remmen, H, Richardson, A, et al. (2005) Methionine oxidation and aging. Biochim Biophys Acta 1703, 135140.
9 Swennen, Q, Decuypere, E & Buyse, J (2007) Implications of dietary macronutrients for growth and metabolism of broiler chickens. World's Poult Sci J 63, 541556.
10 Carsia, RV, Weber, H & Lauterio, TJ (1988) Protein malnutrition in the domestic fowl induces alterations in adrenocortical function. Endocrinology 122, 673680.
11 Lin, H, Decuypere, E & Buyse, J (2004) Oxidative stress induced by corticosterone administration in broiler chickens (Gallus gallus domesticus). 1. Chronic exposure. Comp Biochem Physiol B Biochem Mol Biol 139, 737744.
12 Lin, H, Decuypere, E & Buyse, J (2004) Oxidative stress induced by corticosterone administration in broiler chickens (Gallus gallus domesticus). 2. Short-term effect. Comp Biochem Physiol B Biochem Mol Biol 139, 745751.
13 Buyse, J, Decuypere, E, Berghman, L, et al. (1992) The effect of dietary protein content on episodic growth hormone secretion and on heat production of male broilers. Br Poult Sci 33, 11011109.
14 Martin-Venegas, R, Geraert, P-A & Ferrer, R (2006) Conversion of the methionine hydroxy analogue, dl-2-hydroxy-(4-methylbio) butanoic acid, to sulphur-containing amino acids in the chicken small intestine. Poult Sci 85, 19321938.
15 Darras, VM, Kotanen, SP, Geris, KL, et al. (1996) Plasma thyroid hormone levels and iodothyronine deiodinase activity following an acute glucocorticoid challenge in embryonic compared with posthatch chickens. Gen Comp Endocrinol 104, 203212.
16 Sugiyama, T, Kawarada, Y & Hirasawa, F (2000) Ceruloplasmin. In Experimental Protocols for Reactive Oxygen and Nitrogen Species, pp. 111113 [Taniguchi, N and Gutteridge, JMC, editors]. New York: Oxford University Press.
17 De Boever, S, Vangestel, C, De Backer, P, et al. (2008) Identification and validation of housekeeping genes as internal control for gene expression in an intravenous LPS inflammation model in chickens. Vet Immunol Immunopathol 122, 312317.
18 Vazquez-Anon, M, Kratzer, D, Gonzalez-Esquerra, R, et al. (2006) A multiple regression model approach to contrast the performance of 2-hydroxy-4-methylthiobutanoic acid and dl-methionine supplementation tested in broiler experiments and reported in the literature. Poult Sci 85, 693705.
19 Kratzer, DD & Littell, RC (2006) Appropriate statistical methods to compare dose responses of methionine sources. Poult Sci 85, 947954.
20 Shutte, JB & De Jong, J (1996) Biological efficacy of dl-methionine hydroxyl analog free acid compared to dl-methionine hydrox analog free acid in broiler chicks as determined by performance and breast meat yield. Agribiol Res 49, 7482.
21 Mercier, Y, Francesch, M, Pérez de Rozas, A, et al. (2007) Effects of methionine sources and NSP enzymes on broiler gut microflora. 16th European Symposium of Poultry Nutrition, August 26–30, 2007 Strasbourg, France, pp. 577580.
22 Govaerts, T, Room, G, Buyse, J, et al. (2000) Early and temporary quantitative food restriction of broiler chickens. 2. Effects on allometric growth and growth hormone secretion. Br Poult Sci 41, 355362.
23 Benzie, IFF (1996) Lipid peroxidation: a review of causes, consequences, measurement and dietary influences. Int J Food Sci Nutr 47, 233261.
24 Park, YS, Suzuki, K, Taniguchi, N, et al. (1999) Gluthatione peroxidase-like activity of caeruloplasmin as an important lung antioxidant. FEBS Lett 458, 133136.
25 Hellstein, Y, Tullson, PC, Richter, EA, et al. (1997) Oxidation of urate in human skeletal muscle during exercise. Free Radic Biol Med 22, 169174.
26 Simoyi, MF, Dyke, KV & Klandorf, H (2002) Manipulation of plasma uric acid in broiler chicks and its effect of leucocyte oxidative injury. Am J Physiol 282, R791R796.
27 Bottje, WG & Wideman, RF Jr (1995) Potential role of free radicals in the pathogenesis of pulmonary hypertension syndrome. Poult Avian Biol Rev 6, 211231.

Keywords

Effects of dietary protein content and 2-hydroxy-4-methylthiobutanoic acid or dl-methionine supplementation on performance and oxidative status of broiler chickens

  • Quirine Swennen (a1), Pierre-André Geraert (a2), Yves Mercier (a2), Nadia Everaert (a1), Anneleen Stinckens (a1), Hilke Willemsen (a1), Yue Li (a1), Eddy Decuypere (a1) and Johan Buyse (a1)...

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed