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Effects of dietary coconut oil on apolipoprotein B synthesis and VLDL secretion by calf liver slices

Published online by Cambridge University Press:  09 March 2007

Dominique Gruffat-mouty ,
Benoît Graulet ,
Denys Durand ,
Marie Elisabeth Samson-Bouma  and
Dominique Bauchart
Dominique Gruffat-mouty*
Affiliation:
Unité de Recherches sur les Herbivores, Équipe Nutriments et Métabolismes, Institut National de Recherche Agronomique, Centre de Recherches Clermont Ferrand - Theix, 63122 Saint Genès Champanelle, France
Benoît Graulet
Affiliation:
Unité de Recherches sur les Herbivores, Équipe Nutriments et Métabolismes, Institut National de Recherche Agronomique, Centre de Recherches Clermont Ferrand - Theix, 63122 Saint Genès Champanelle, France
Denys Durand
Affiliation:
Unité de Recherches sur les Herbivores, Équipe Nutriments et Métabolismes, Institut National de Recherche Agronomique, Centre de Recherches Clermont Ferrand - Theix, 63122 Saint Genès Champanelle, France
Marie Elisabeth Samson-Bouma
Affiliation:
INSERM U 327, Faculté de Médecine Xavier Bichat, 16 rue Henri Huchard, BP 416, 75870 Paris Cedex 18, France
Dominique Bauchart
Affiliation:
Unité de Recherches sur les Herbivores, Équipe Nutriments et Métabolismes, Institut National de Recherche Agronomique, Centre de Recherches Clermont Ferrand - Theix, 63122 Saint Genès Champanelle, France
*
*Corresponding author: Dr Dominique Gruffat-Mouty, fax +33 04 73 62 46 39, email gruffat@clermont.inra.fr
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Abstract

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Incorporation of coconut oil (CO) rich in lauric acid into the milk diet induces a lipid infiltration of the liver (steatosis) in 1-month-old calves. Among possible steps involved in diet-induced liver steatosis, the ability of the calf liver to synthesize apolipoprotein (Apo) B and to secrete it as part of VLDL particles was investigated. Liver samples were taken from calves fed for 17 d on a conventional milk replacer containing CO (n 5) and beef tallow (BT, n 4) as reference. Samples were cut into slices 0·5 mm thick and subsequently incubated for 12 h in a medium containing a [35S]methionine–[35S]cysteine mix and 0·8 mM-sodium laurate or oleate, the major fatty acids of CO and BT diets respectively. Concentrations of total [35S]proteins, [35S]albumin and [35S]ApoB in liver cells were 2-fold lower (P=0·08, 0·0004 and 0·03 respectively) in CO- than in BT-fed calves. Although the total amount of proteins secreted (including albumin) was similar in both groups of calves, the amount of VLDL-[35S]Apo secreted was 2-fold lower (P=0·004) in CO- than in BT-fed calves. These results suggest that a CO-enriched milk diet induces in preruminant calves a lipid infiltration of the liver by decreasing ApoB synthesis, leading to a reduction in secretion of VLDL particles.

Keywords

Liver slicesCoconut oilApolipoprotein BVLDLCalf
Type
Research Article
Information
British Journal of Nutrition , Volume 86 , Issue 1 , July 2001 , pp. 13 - 19
Copyright
Copyright © The Nutrition Society 2001

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