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Effects of cyclamate and food restriction on various metabolites in the rat

Published online by Cambridge University Press:  09 February 2010

L. Prosky  and
R. G. O'Dell
L. Prosky
Affiliation:
Division of Nutrition, Food and Dmg Administration, US Department of Health, Education, and Welfare, Washington, DC 20204, U S A
R. G. O'Dell
Affiliation:
Division of Nutrition, Food and Dmg Administration, US Department of Health, Education, and Welfare, Washington, DC 20204, U S A
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Abstract

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1. Rats were given stock chow diets containing several levels of calcium cyclamate to study the effects on growth and on some metabolites in liver and blood.

2. Levels up to 1% in the diet produced diarrhoea without affecting body-weight. At a level of 3% in the diet, body-weight was decreased by 12% in 8 weeks.

3. No changes were noted in liver protein, lipid and RNA-P and serum protein and lipid. 14CO2 excretion during the 1st hour after [14C]glucose administration also remained unchanged.

4. Adult rats weighing 500 g were given, at a restricted intake, a diet with variations in its fat and cyclamate contents.

5. During 15 weeks, the animals given the fat-supplemented diet plus cyclamate lost twice as much weight as controls without cyclamate and also excreted 20% more 14CO2. When the food intake was further restricted for 15 weeks weight losses in all groups were the same.

6. Serum lipid and free cholesterol concentrations were lowered in the cyclamate group. 14CO2 excretion for this group was 35% higher than for controls, indicating increased metabolic activity.

7. Concentrations of aspartate, glutamate, lactate, succinate, malate and glycerol-I-phosphate in liver were within normal limits. There were indications of decreased levels of lactate and succinate in cyclamate-fed rats which could be associated with aerobiosis and increased metabolic activity.

Type
General Nutrition
Information
British Journal of Nutrition , Volume 27 , Issue 2 , March 1972 , pp. 353 - 363
Copyright
Copyright © The Nutrition Society 1972

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