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Effects of a mixture of organisms, Lactobacillus acidophilus or Streptococcus faecalis on cholesterol metabolism in rats fed on a fat- and cholesterol-enriched diet

Published online by Cambridge University Press:  09 March 2007

Michihiro Fukushima  and
Masuo Nakano
Michihiro Fukushima
Affiliation:
Department of Bioresource Chemistry, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080, Japan
Masuo Nakano
Affiliation:
Department of Bioresource Chemistry, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080, Japan
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Abstract

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The effect of a mixture of organisms (a probiotic mixture) comprising Bacillus, Lactobacillus, Streptococcus, Clostridium, Saccharomyces and Candida(107–8 colony-forming units/g rice bran of each component) on lipid metabolism was compared with that of L. acidophilus and that of S. faecalis. There were four treatment groups: rice bran (control), the mixture of organisms, L. acihphifus or S. faecds (30g/kg) were given to rats in a fat- and cholesterol-enriched diet for 4 weeks. The serum total cholesterol concentration of the group fed on the mixture of organisms was reduced by 15–33% compared with the other groups at the end of the 4week feeding period (P < 0·05). This group also had a lower hepatic cholesterol concentration (36–44%) than the two single-bacteria groups (P < 0·05). 3-Hydmxy-3-methylglutaryl-Co A reductase (NADPH; EC 1.1.1.34) activities of the mixed-organism and L. acidophifus groups were significantly lower (61–63%) than those of the other groups (P < 0·05); the activity of the S. faecalis group was also signikantly lower (42%) than that of the control group (P < 0·05). The faecal cholesterol and bile acid concentrations of the mixed-organism group increased compared with those of the L. acidophilus and S. faecalis groups (P < 0·05). The capacity of the mixed- organism cells to bind bile salt in vitro was significantly higher (approximately 50%) than that of the singlebacteria cells (P < 0·05). On the other hand, cholesterol micelle formation for the mixed-organism cells was significantly (approximately 9%) lower than that of the singlebacteria cells (P < 0·05). These results indicate that the mixture of organisms decreased the synthesis of cholesterol in the liver and increased the loss of steroids from the intestine, in rats. Thus, the mixture of organisms had a hypocholeaterolaemic role

Keywords

ProbioticsLactobacillus acidophilusStreptococcus faecalisCholesterol metabolismHMG-CoA reductase
Type
General Nutrition
Information
British Journal of Nutrition , Volume 76 , Issue 6 , December 1996 , pp. 857 - 867
Copyright
Copyright © The Nutrition Society 1997

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