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Effect of viscosity on digestion of nutrients in conventional and germ-free chicks

Published online by Cambridge University Press:  09 March 2007

D. J. Langhout ,
J. B. Schutte ,
J. de Jong ,
H. Sloetjes ,
W. A. Verstegen  and
S. Tamminga
D. J. Langhout*
Affiliation:
TNO Nutrition and Food Research Institute, Department of Animal Nutrition and Physiology (ILOB), PO Box 15, 6700 AA Wageningen, The Netherlands
J. B. Schutte
Affiliation:
TNO Nutrition and Food Research Institute, Department of Animal Nutrition and Physiology (ILOB), PO Box 15, 6700 AA Wageningen, The Netherlands
J. de Jong
Affiliation:
TNO Nutrition and Food Research Institute, Department of Animal Nutrition and Physiology (ILOB), PO Box 15, 6700 AA Wageningen, The Netherlands
H. Sloetjes
Affiliation:
Institute for Animal Science and Health (ID-DLO), PO Box 65, 8200 AB Lelystad, The Netherlands
W. A. Verstegen
Affiliation:
Department of Animal Nutrition Wageningen Agricultural University, PO Box 338, 6700 AH Wageningen, The Netherlands
S. Tamminga
Affiliation:
Department of Animal Nutrition Wageningen Agricultural University, PO Box 338, 6700 AH Wageningen, The Netherlands
*
*Corresponding author: Dr D. J. Langhout, present address Provimi BV, PO Box 5063, 3008 AB Rotterdam, The Netherlands, fax +31 10 485 0297, email langhout@provimi.com
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Abstract

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A study was conducted with conventional and germ-free broiler chicks to obtain more information on the role of the intestinal microflora in the anti-nutritive effects of NSP in broiler chicks. As the NSP source, highly methylated citrus pectin (HMC) was used at a dose level of 30 g/kg in a maize-based diet. The diets fed to the germ-free chicks were γ-irradiated, whereas those fed to the conventional chicks were not. Feeding the HMC diet to conventional birds depressed weight gain and food utilization (P < 0·05), whereas in germ-free birds only weight gain was reduced (P < 0·05). Feeding the HMC diet to conventional birds reduced digestibilities of energy and starch at the end of the jejunum. Ileal digestibilities of starch and energy were not strongly affected when birds were fed on the HMC-containing diet. Faecal digestibilities of organic matter, crude fat, starch and amino acids, N retention and metabolizable energy were reduced when conventional chicks were fed on the HMC diet. Feeding the HMC diet to germ-free birds hardly affected faecal digestibility of nutrients and N retention, whereas metabolizable energy was increased. Feeding the HMC diet to conventional or germ-free birds increased the viscosity of the digesta in the small intestine. This increase in digesta viscosity was more pronounced in conventional than in germ-free birds. The pH of ileal digesta was reduced when HMC was added to the diet of conventional chicks, but not in germ-free chicks. Feeding the HMC diet to conventional birds markedly affected morphology of the gut wall, whereas in germ-free chicks very little effect was found on gut morphology. Based on the results of the present study, it is concluded that the gastrointestinal microflora mediates the magnitude of the anti-nutritive effects of HMC in broiler chicks. However, the exact role of the microflora in chicks in the magnitude of the anti-nutritional effects of HMC could not be derived from the present study, since the results might have been influenced by γ-irradiation of the diets fed to the germ-free chicks.

Keywords

Intestinal microfloraCitrus pectinGerm-free broiler chicks
Type
Research Article
Information
British Journal of Nutrition , Volume 83 , Issue 5 , May 2000 , pp. 533 - 540
Copyright
Copyright © The Nutrition Society 2000

References

Agricultural Research Council (1981) The Nutrient Requirement of Pigs. Slough: Commonwealth Agricultural Bureaux.Google Scholar
Antoniou, T and Marquardt, RR (1982) Utilization of rye diets by chicks as affected by lipid type and level and penicillin supplementation. Poultry Science 61, 107–116.CrossRefGoogle Scholar
Antoniou, T, Marquardt, RR and Cansfield, PS (1981) Isolation, partial characterization and antinutritional activity of a factor (pentosans) in rye grain. Journal of Agricultural and Food Chemistry 28, 1240–1247.CrossRefGoogle Scholar
Association of Official Analytical Chemists (1984) Official Methods of Analysis, 14th ed. Washington, DC: AOAC.Google Scholar
Bedford, MR and Classen, HL (1993) An. in vitro assay for prediction of broiler intestinal viscosity and growth when fed rye-based diets in the presence of exogenous enzymes. Poultry Science 72, 137–143.CrossRefGoogle Scholar
Campbell, GL, Campbell, LD and Classen, HL (1983) Utilisation of rye by chickens; effect of microbial status, diet gamma irradiation and sodium taurocholate supplementation. British Poultry Science 24, 191–203.CrossRefGoogle ScholarPubMed
Campbell, GL, Classen, HL and Ballange, GM (1986) Gamma irradiation treatment of cereal grains for chick diets. Journal of Nutrition 116, 560–569.CrossRefGoogle ScholarPubMed
Campbell, GL, Classen, HL, Reichert, RD and Campbell, LD (1983) Improvement of the nutritive value of rye for broiler chickens by gamma irradiation-induced viscosity reduction. British Poultry Science 24, 205–212.CrossRefGoogle ScholarPubMed
Choct, M and Annison, G (1990) Anti-nutritive activity of wheat pentosans in broiler diets. British Poultry Science 31, 811–821.CrossRefGoogle ScholarPubMed
Choct, M and Annison, G (1992) The inhibition of nutrient digestion by wheat pentosans. British Journal of Nutrition 67, 123–132.CrossRefGoogle ScholarPubMed
Choct, M, Hughes, RJ, Wang, J, Bedford, MR, Morgan, AJ and Annison, G (1996) Increased small intestinal fermentation is partly responsible for the anti-nutritive activity of non-starch polysaccharides in chickens. British Poultry Science 37, 609–621.CrossRefGoogle ScholarPubMed
Classen, HL, Campbell, GL, Rosnagel, BG, Bhatty, R and Reichert, RD (1985) Studies on the use of hulles barley in chick diets: deleterious effects and methods of alleviation. Canadian Journal of Animal Science 65, 725–733.CrossRefGoogle Scholar
Coates, ME, Cole, CB, Fuller, R, Houghton, SB and Yokota, H (1981) The gut microflora and the uptake of glucose from the small intestine of the chick. British Poultry Science 22, 289–294.CrossRefGoogle ScholarPubMed
Dutch Bureau of Livestock Feedingstuffs (1994) Chemical Composition, Digestibility and Energy Value of Feed Ingredients. Lelystad, The Netherlands: Bureau of Livestock Feedingstuffs.Google Scholar
Edwards, CA, Johnson, II and Read, NW (1988) Do viscous polysaccharides slow absorption by inhibiting diffusion or convection?. European Journal of Clinical Nutrition 42, 307–312.Google ScholarPubMed
European Union (1984) Determination of crude oils and fats. Official Journal of the European Communities EU 18.1.84 no. 15/29-30.Google Scholar
Fengler, AI and Marquardt, RR (1988) Water-soluble pentosans from rye: II. Effects on rate of dialysis and on the retention of nutrients by the chick. Cereal Chemistry 65, 298–302.Google Scholar
Furuse, M and Yokota, H (1985) Effect of the gut microflora on chick growth and utilization of protein and energy at different concentrations of dietary protein. British Poultry Science 26, 97–104.CrossRefGoogle ScholarPubMed
Garrett, RL and Young, RJ (1975) Effect of micelle formation on the absorption of neutral fat and fatty acids by the chicken. Journal of Nutrition 105, 827–838.CrossRefGoogle ScholarPubMed
Gee, JMLee-Finglas, W, Wortley, GW and Johnson, IT (1996) Fermentable carbohydrates elevate plasma enteroglucagon but high viscosity is also necessary to stimulate small bowel mucosal cell proliferation in rats. Journal of Nutrition 126, 373–379.CrossRefGoogle ScholarPubMed
Hill, FW and Anderson, DL (1958) Comparison of metabolizable energy and productive energy determinations with growing chicks. Journal of Nutrition 64, 587–603.CrossRefGoogle ScholarPubMed
Hylemond, PB (1985) Metabolism of bile acids in intestinal microflora. In Sterols and Bile Acids: New Comprehensive Biochemistry, pp. 331–343 [Danielsen, H and Sjovall, J, editors]. Amsterdam: Elsevier Science Publishers B.V.CrossRefGoogle Scholar
Just, A, Fernande, JA and Jorgensen, H (1983) The net energy value of diets for growth in pigs in relation to the fermentative processes in the digestive tract and the site of absorption of the nutrients. Livestock Production Science 10, 171–186.CrossRefGoogle Scholar
Langhout, DJ and Schutte, JB (1996) Nutritional implications of pectins in chicks in relation to esterification and origin of pectins. Poultry Science 75, 1236–1242.CrossRefGoogle ScholarPubMed
Langhout, DJ, Schutte, JB, Tangerman, AVerstraten, AJMA, Van Schaik, A and Beelen, G (1999) Effect of dietary viscous polysaccharides on the ileal microflora and on bile acid deconjugation in broiler chicks British Poultry Science.Google Scholar
Langhout, DJ, Schutte, JBVan Leeuwen, P, Wiebenga, J and Tamminga, S (1998) Effect of dietary high- and low-methylated citrus pectin on activity of the ileal microflora and morphology of the small intestinal wall of broiler chicks. British Poultry Science 40, 340–347.CrossRefGoogle Scholar
MacAuliffe, T, Zaviezo, D and Bayley, HS (1979) Effect of gamma irradiation, fractionation, and penicillin supplementation on the rachitogenic activity of rye for chicks. Poultry Science 58, 329–332.CrossRefGoogle ScholarPubMed
McBurney, MI, Horvath, PJ, Jeraci, LJ and Van Soest, PJ (1985) Effect of. in vitro fermentation using human faecal inoculum on the water-holding capacity of dietary fibre. British Journal of Nutrition 53, 17–24.CrossRefGoogle ScholarPubMed
Moore, S (1963) On the determination of cysteic as cystic acid. Journal of Biology and Chemistry 238, 235–237.CrossRefGoogle Scholar
National Research Council (1994) Nutrient Requirements of Poultry, 9th revised ed. Washington DC: National Academy of Sciences.Google Scholar
Nederlands Normalisatie Instituut (1992) NNI-catalogus I. Delft, The Netherlands: NNI.Google Scholar
Norusis, MJ (1992) SPSS/PC+ Base System User's Guide, version 5.0. Chicago, IL: SPSS Inc.Google Scholar
Patel, MB, Jami, MS and McGinnis J (1980) Effect of gamma irradiation, penicillin, and/or pectic enzyme on chick growth depression and fecal stickiness caused by rye, citrus pectin, and guar gum. Poultry Science 59, 2105–2110.CrossRefGoogle ScholarPubMed
Salter, DN and Coates, ME (1974) The utilization of protein and excretion of uric acid in germ-free and conventional chicks. British Journal of Nutrition 31, 307–318.CrossRefGoogle ScholarPubMed
Slump, P (1969) Characterization of nutritive value of food protein by amino acid composition. PhD Thesis, Free University of Amsterdam.Google Scholar
Snedecor, GW & Cochran, WG (1980) Statistical Methods, 7th ed. Ames, IA: The Iowa State University Press.Google Scholar
Van Es, AJH (1974) Energy utilization of low digestibility carbohydrates. In Low Digestibility Carbohydrates, pp. 121–127 [Leegwater, DC, Feron, VJ and Hermus, RJJ, editors]. Wageningen: Pudoc Press.Google Scholar
Van der Klis, JD, Verstegen, MWA and Van Voorst, A (1993) Effect of a soluble polysaccharide (Carboxy Methyl Cellulose) on the absorption of minerals from the gastrointestinal tract of broilers. British Poultry Science 34, 377–389.Google ScholarPubMed
Wagner, DD and Thomas, OP (1978) Influence of diets containing rye or pectin on the intestinal flora of chicks. Poultry Science 57, 971–975.CrossRefGoogle ScholarPubMed
Ward, AT and Marquardt, RR (1983) The effect of saturation, chain length of pure triglycerides, and age of bird on the utilization of rye diets. Poultry Science 62, 1054–1062.CrossRefGoogle ScholarPubMed
White, WB, Bird, HR, Sunde, ML, Prentice, N, Burger, WC and Marlett, JA (1981) The viscosity interaction of barley β-glucan with Trichoderma viride cellulase in the chick intestine. Poultry Science 62, 853–862.CrossRefGoogle Scholar