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Effect of isomalt consumption on faecal microflora and colonic metabolism in healthy volunteers

  • A. Gostner (a1), M. Blaut (a2), V. Schäffer (a1), G. Kozianowski (a3), S. Theis (a3), M. Klingeberg (a3), Y. Dombrowski (a1), D. Martin (a3), S. Ehrhardt (a3), D. Taras (a2), A. Schwiertz (a2), B. Kleessen (a2), H. Lührs (a1), J. Schauber (a1), D. Dorbath (a1), T. Menzel (a1) and W. Scheppach (a1)...


Due to its low digestibility in the small intestine, a major fraction of the polyol isomalt reaches the colon. However, little is known about effects on the intestinal microflora. During two 4-week periods in a double-blind, placebo-controlled, cross-over design, nineteen healthy volunteers consumed a controlled basal diet enriched with either 30g isomalt or 30g sucrose daily. Stools were collected at the end of each test phase and various microbiological and luminal markers were analysed. Fermentation characteristics of isomalt were also investigated in vitro. Microbiological analyses of faecal samples indicated a shift of the gut flora towards an increase of bifidobacteria following consumption of the isomalt diet compared with the sucrose diet (P<0·05). During the isomalt phase, the activity of bacterial β-glucosidase decreased (P<0·05) whereas β-glucuronidase, sulfatase, nitroreductase and urease remained unchanged. Faecal polyamines were not different between test periods with the exception of cadaverine, which showed a trend towards a lower concentration following isomalt (P=0·055). Faecal SCFA, lactate, bile acids, neutral sterols, N, NH3, phenol and p-cresol were not affected by isomalt consumption. In vitro, isomalt was metabolized in several bifidobacteria strains and yielded high butyrate concentrations. Isomalt, which is used widely as a low-glycaemic and low-energy sweetener, has to be considered a prebiotic carbohydrate that might contribute to a healthy luminal environment of the colonic mucosa.

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Corresponding author

*Corresponding author:Dr Andrea Godtner, fax +49 931 201 36 101, email


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Alles, MS, Hartemink, R, Meyboom, S, Harryvan, JL, Van Laere, KM, Nagengast, FM & Hautvast, JGEffect of transgalactooligosaccharides on the composition of the human intestinal microflora and on putative risk markers for colon cancer. Am J Clin Nutr (1999) 69, 980991.
Balmer, SE & Wharton, BADiet and fecal flora of the new born: breast milk and infant formula. Arch Dis Child (1989) 64, 16721677.
Bär, A Factorial calculation model for the estimation of the physiological caloric value of polyols. In Proceedings of the International Symposium on Caloric Evaluation of Carbohydrates, pp. 209257 [N, Hosoxa, editor]. Tokyo: Research Foundation for Sugar Metabolism (1990).
Batta, AK, Salen, G, Rapole, KR, Batta, M, Batta, P & Alberts, EDHighly simplified method for gas-liquid chromatographic quantitation of bile acids and sterols in human stool. J Lipid Res (1999) 40, 11481154.
Bartram, HP, Scheppach, W, Heid, C, Fabian, C & Kasper, HEffect of starch malabsorption on fecal bile acids and neutral sterols in humans: possible implications for colonic carcinogenesis. Cancer Res (1991) 51, 42384242.
Benamouzig, R, Mahé, S, Luengo, C, Rautureau, J & Tomé, DFasting and postprandial polyamine concentrations in human digestive lumen. Am J Clin Nutr (1997) 65, 766770.
Bezkorovainy, AProbiotics: determinants of survival and growth in the gut. Am J Clin Nutr (2001) 73, Suppl. 3, 99S405S.
Blei, ATDiagnosis and treatment of hepatic encephalopathy. Baillieres Best Pract Res Clin Gastroenterol (2000) 14, 959974.
Bone, E, Tamm, A & Hill, MThe production of urinary phenols by gut bacteria and their possible role in the causation of large bowel cancer. Am J Clin Nutr (1976) 29, 14481454.
Bouhnik, Y, Vahedi, K, Achour, L, Attar, A, Salfati, J, Pochart, P, Marteau, P, Flourie, B, Bornet, F & Rambaud, JCShortchain fructo-oligosaccharide administration dose-dependently increases fecal bifidobacteria in healthy humans. J Nutr (1999) 129, 113116.
Brighenti, F, Casiraghi, C, Canzi, E & Ferari, AEffect of consumption of a ready-to eat breakfast cereal containing inulin on the intestinal milieu and blood lipids in healthy male volunteers. Eur J Clin Nutr (1999) 53, 726733.
Bryan, GTThe role of urinary tryptophan metabolites in the etiology of bladder cancer. Am J Clin Nutr (1971) 24, 841847.
Buddington, RK, Williams, CH, Chen, SC & Witherly, SADietary supplement of neosugar alters the fecal flora and decreases activities of some reductive enzymes in human subjects. Am J Clin Nutr (1996) 63, 709716.
Cummings, JH, Macfarlane, GT & Englyst, HNPrebiotic digestion and fermentation. Am J Clin Nutr (2001) 73, Suppl. 4, 15S420S.
Edwards, CA & Prrett, AMIntestinal flora during the first months of life: new perspectives. Br J Nutr (2002) 88, Suppl., S11S18.
Franks, AH, Harmsen, HJM, Raangs, GC, Jansen, GJ, Schut, F & Welling, GVariations of bacterial populations in human faeces measured by fluorescent in situ hybridisation with groupspecific 16S rRNA-targeted oligonucleotide probes. Appl Environ Microbiol (1998) 64, 33363345.
Gee, JM, Cooke, D, Gorick, S, Wortley, GM, Greenwood, RH, Zumbe, A & Johnson, ITEffects of conventional sucrose-based, fructose-based and isomalt-based chocolates on postprandial metabolism in non-insulin-dependent diabetics. Eur J Clin Nutr (1991) 45, 561566.
Gehring, F & Karle, EJSweetening agent, Palatinit under specific consideration as to microbiological and caries-prophylactic aspects. Z Ernahrungswiss (1981) 20, 96106.
Giaffer, MH, Holdsworth, CD & Duerden, BIThe assessment of faecal flora in patients with inflammatory bowel disease by a simplified bacteriological technique. J Med Microbiol (1991) 35, 238243.
Gibson, GR, Beatty, ER, Wang, X & Cummings, JHSelective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology (1995) 108, 975982.
Gibson, GR & Roberfroid, MBDietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr (1995) 125, 14011412.
Gostner, A, Schäffer, V, Theis, S et al. , Effects of isomalt consumption on gastrointestinal and metabolic parameters in healthy volunteers. Br J Nutr (2005) 94, 575581.
Grasten, SM, Juntunen, KS, Poutanen, KS, Gylling, HK, Miettinen, TA & Mykkanen, HMRye bread improves bowel function and decreases the concentrations of some compounds that are putative colon cancer risk markers in middle-aged women and men. J Nutr (2000) 130, 22152221.
Harmsen, HJM, Elferrich, P, Schut, F & Welling, GWA 16S rRNA-targeted probe for detection of lactobacilli and enterococci in faecal samples by fluorescent in situ hybridisation. Microb Ecol Health Dis (1999) 11, 312.
Harmsen, HJ, Raangs, GC, He, T, Degener, JE & Welling, GExtensive set of 16S rRNA-based probes for detection of bacteria in human feces. Appl Environ Microbiol (2002a) 68, 29822990.
Harmsen, HJ, Wildeboer-Veloo, AC, Grijpstra, J, Knol, J, Degener, JE & Welling, GWDevelopment of 16S rRNA-based probes for the Coriobacterium group and the Atopobium cluster and their application for enumeration of Coriobacteriaceae in human feces from volunteers of different age groups. Appl Environ Microbiol (2000) 66, 45234527.
Harmsen, HJM, Raangs, GC, Franks, AH, Wildeboer-Veloo, AC & Welling, GWThe effect of the prebiotic inulin and the prebiotic Bifidobacterium longum on the fecal microflora of healthy volunteers measured by FISH and DGGE. Microbiol Ecol Health Dis (2002b) 14, 211219.
Hold, GL, Schwiertz, A, Aminov, R, Blaut, M & Flint, HJOligonucleotide probes that detect quantitatively significant groups of butyrate-producing bacteria within the human fecal flora. Appl Environ Microbiol (2003) 69, 43204324.
Hütter, R, Böswart, F & Irsigler, KInsulinverbrauch von Typ-IDiabetikern nach oraler Gabe von Isomalt. Akt Ernähr (1993) 18, 149154.
Hylla, S, Gostner, A, Dusel, G, Anger, H, Bartram, HP, Christl, SU, Kasper, H & Scheppach, WEffects of resistant starch on the colon in healthy volunteers: possible implications for cancer prevention. Am J Clin Nutr (1998) 67, 136142.
Johanson, GK, Ottova, L & Gustafsson, JAShift from a mixed diet to a lactovegetarian diet: influence on some cancer-associated intestinal bacterial enzyme activities. Nutr Cancer (1990) 14, 239246.
Kleessen, B, Hartmann, L & Blaut, MOligofructose and longchain inulin influence on the gut microbial ecology of rats associated with a human faecal flora. Br J Nutr (2001) 86, 291300.
Kleessen, B, Sykura, B, Zunft, HJ & Blaut, MEffects of inulin and lactose on fecal microflora, microbial activity, and bowel habit in elderly constipated persons. Am J Clin Nutr (1997) 65, 13971402.
Kruse, HP, Kleessen, B & Blaut, MEffects of inulin on faecal bifidobacteria in human subjects. Br J Nutr (1999) 82, 375382.
Langendijk, PS, Schut, F, Jansen, GJ, Raangs, GC, Kamphuis, GR, Wilkinson, MHF & Welling, GWQuantitative fluorescence in situ hybridisation of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples. Appl Environ Microbiol (1995) 61, 30693075.
Langkilde, AM, Andersson, H, Schweizer, TF & Wursch, PDigestion and absorption of sorbitol, maltitol and isomalt from the small bowel. A study in ileostomy subjects. Eur J Clin Nutr (1994) 48, 768775.
Lin, HC & Visek, WJColon mucosal cell damage by ammonia in rats. J Nutr (1991) 121, 887893.
Linskens, RK, Huijsdens, XW, Savelkoul, PH, Vandenbroucke-Grauls, CM& Meuwissen, SGThe bacterial flora in inflammatory bowel disease: current insights in pathogenesis and the influence of antibiotics and probiotics. Scand J Gastroenterol (2001) 234, Suppl., 2940.
Livesey, GHealth potential of polyols as sugar replacers, with emphasis on low glycaemic properties. Nutr Res Rev (2003) 16, 163191.
McBain, AJ & Macfarlane, GTEcological and physiological studies on large intestinal bacteria in relation to production of hydrolytic and reductive enzymes involved in formation of genotoxic metabolites. J Med Microbiol (1998) 47, 407416.
McBain, AJ & Macfarlane, GTModulation of genotoxic enzyme activities by non-digestible oligosaccharide metabolism in in-vitro human gut bacterial ecosystems. J Med Microbiol (2001) 50, 833842.
Mallett, AK, Rowland, IR & Farthing, MJDietary modification of intestinal bacterial enzyme activities — potential formation of toxic agents in the gut. Scand J Gastroenterol (1987) 129, Suppl., 251257.
Manz, W, Amann, R, Ludwig, W, Vancanneyt, M & Schleifer, K-HApplication of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum cytophaga- flavobacter-bacteroides in the natural environment. Microbiology (1996) 142, 10971106.
Manz, W, Amann, R, Ludwig, W, Wagner, M & Schleifer, K-HPhylogenetic oligodeoxynucleotide probes for the major subclasses of proteobacteria: problems and solutions. Syst Appl Microbiol (1992) 15, 593600.
Marteau, P, Seksik, P & Jian, RProbiotics and intestinal health effects: a clinical perspective. Br J Nutr (2002) 88, Suppl., S51S57.
Meier, H, Amann, R, Ludwig, W & Schleifer, K-HSpecific oligonucleotide probes for in situ detection of a major group of grampositive bacteria with low DNA G + C content. Syst Appl Microbiol (1999) 22, 186196.
Menne, E, Guggenbuhl, N & Roberfroid, MFn-type chicory inulin hydrolysate has a prebiotic effect in humans. J Nutr (1997) 130, 11971199.
Noack, J, Kleessen, B, Proll, J, Dongowski, G & Blaut, MDietary guar gum and pectin stimulate intestinal microbial polyamine synthesis in rats. J Nutr (1998) 128, 13851391.
Petzoldt, R, Lauer, P, Spengler, M & Schoffling, KPalatinite in type II diabetics. Effect on blood-glucose, serum-insulin, C-peptide and free fatty acids. Dtsch Med Wochenschr (1982) 107, 19101913.
Pistoli, S, Smejkal, C, McCartney, A & Gibson, GRDifferences in the faecal flora of healthy individual and patients with irritable bowel syndrome, and in vitro effects of a synbiotic upon gut flora composition Clin Nutr (2003) 22, 60.
Poulsen, IK, Licht, TR, Rang, C, Krogfelt, KA & Molin, SPhysiological state of Escherichia coli BJ4 growing in the large intestines of streptomycin-treated mice. J Bacteriol (1995) 177, 58405845.
Rao, AVThe prebiotic properties of oligofructose at low intake levels. Nutr Res (2001) 21, 843848.
Roediger, WEUtilization of nutrients by isolated epithelial cells of the rat colon. Gastroenterology (1982) 83, 424429.
Roller, C, Wagner, M, Amann, R, Ludwig, W & Schleifer, K-HIn situ probing of Gram-positive bacteria with high DNA G + C content using 23S rRNA-targeted oligonucleotides. Microbiology (1994) 140, 28492858.
Scheppach, WEffects of short chain fatty acids on gut morphology and function. Gut (1994) 35, S35S38.
Scheppach, W, Luehrs, H & Menzel, TBeneficial health effects of low-digestible carbohydrate consumption. Br J Nutr (2001) 85, Suppl. 1, S23S30.
Schwiertz, A, Le Blay, G & Blaut, MQuantification of different Eubacterium spp. in human fecal samples with species-specific 16S rRNA-targeted oligonucleotid probes. Appl Environ Microbiol (2000) 66, 375382.
Suau, A, Rochet, V, Sghir, A, Gramet, G, Brewaeys, S, Sutren, M, Rigottier-Gois, L & Dore, JFusobacterium praunitzii and related species represent a dominant group within the human fecal flora. Syst Appl Microbiol (2001) 24, 139145.
Tuohy, AM, Finlay, RK, Wynne, AG& Gibson, GRA human volunteer study on the prebiotic effects of HP-inulin — fecal bacteria enumerated using fluorescent in situ hybridisation (FISH). Anaerobe (2001a) 7, 113119.
Tuohy, KM, Kolida, S, Lustenberger, AM & Gibson, GRThe prebiotic effects of biscuits containing partially hydrolysed guar gum and fructo-oligosaccharides — a human volunteer study. Br J Nutr (2001b) 86, 341348.
van de Kamer, JH, Bokkel Ten Huining, H & Weyers, HAEinfache Methode zur Bestimmung des Fettgehalts im Stuhl. J Biol Chem (1949) 177, 347 & 350.
Van Loo, J, Cummings, J, Delzenne, N et al. Functional food properties of non-digestible oligosaccharides: a consensus report from the ENDO project (DGXII AIRII-CT94-1095). Br J Nutr (1999) 81, 121132.
van Weerden, EJ & Huisman, JThe digestion process of the sugar alcohol isomalt in the intestinal tract of the pig. 1. Studies with administration of isomalt in the feed. Br J Nutr (1993) 69, 455466.
Velazquez, OC, Lederer, HM & Rombeau, JLButyrate and the colonocyte. Implications for neoplasia Dig Dis Sci (1996) 41, 727739.
Weber, FL JrEffects of lactulose on nitrogen metabolism. Scand J Gastroenterol (1997) 222, Suppl., 8387.
Wheeler, LA, Soderberg, FB & Goldman, PThe relationship between nitrogroup reduction and the intestinal microflora. J Pharmacol Exp Ther (1975) 194, 135144.
Wise, A, Mallet, AK & Rowland, IRDietary fibre, bacterial metabolism and toxicity of nitrate in the rat. Xenobiotica (1982) 12, 111118.
Zhong, J, Luo, B, Xiang, M, Liu, H, Zhai, Z, Ang, T & Craig, SStudies on the effects of polydextrose intake on physiologic functions in Chinese people. Am J Clin Nutr (2000) 72, 15031509.


Effect of isomalt consumption on faecal microflora and colonic metabolism in healthy volunteers

  • A. Gostner (a1), M. Blaut (a2), V. Schäffer (a1), G. Kozianowski (a3), S. Theis (a3), M. Klingeberg (a3), Y. Dombrowski (a1), D. Martin (a3), S. Ehrhardt (a3), D. Taras (a2), A. Schwiertz (a2), B. Kleessen (a2), H. Lührs (a1), J. Schauber (a1), D. Dorbath (a1), T. Menzel (a1) and W. Scheppach (a1)...


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