Hostname: page-component-8448b6f56d-dnltx Total loading time: 0 Render date: 2024-04-17T23:14:50.327Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of environmental temperature on prandial changes in leucocyte sodium transport in man

Published online by Cambridge University Press:  09 March 2007

L. L. Ng  and
T. D. R. Hockaday
L. L. Ng
Affiliation:
Department of Clinical Pharmacology, Radcliffe Infirmary, Oxford OX2 6HE
T. D. R. Hockaday
Affiliation:
Sheikh Rashid Diabetes Unit, Radcliffe Infirmary, Oxford OX2 6HE
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Cellular sodium transport via the Na+, K+-ATPase contributes significantly to daily energy expenditure. The effect of a meal on leucocyte Na+ transport and intracellular electrolytes was therefore investigated in lean normal subjects at room temperatures of 23° and 33°, to determine if the Na pump responds to the need for thermogenesis. In the fasting state, the ouabain-sensitive efflux rate which reflects active Na+ transport, and the intracellular electrolytes were similar. At 2 h after eating a 4·2 MJ (1000 kcal) meal, the ouabain-sensitive efflux rate constant rose when the room temperature was 23° but not at 33°. The ouabain-sensitive Na+ efflux rate, an index of active Na+ transport, 0rose post-prandially at 23° only. The post-prandial activation of leucocyte active Na efflux in normal subjects was blunted at higher environmental temperatures, when the need for thermogenesis was reduced.

Keywords

ATPaseEnvironmental temperatureLeucocytesSodium transport
Type
Energy Metabolism
Information
British Journal of Nutrition , Volume 62 , Issue 3 , November 1989 , pp. 639 - 645
Copyright
Copyright © The Nutrition Society 1989

References

REFERENCES

Akanji, A.O., Bruce, M., Frayn, K., Hockaday, T.D.R. & Kaddaha, G.M. (1987). Oral glucose tolerance and ambient temperature in non-diabetic subjects. Diabetologia 30, 431–433.CrossRefGoogle ScholarPubMed
Blaza, S. & Garrow, J.S. (1983). Thermogenic response to temperature, exercise and food stimuli in lean and obese women, studied by 24 h direct calorimetry. British Journal of Nutrition 49, 171–180.Google ScholarPubMed
Chinet, A., Clausen, T. & Girardier, L. (1977). Microcalorimetric determination of energy expenditure due to active sodium-potassium transport in the soleus muscle and brown adipose tissue of the rat. Journal of Physiology 265, 43–61.CrossRefGoogle Scholar
Dauncey, M.J. (1981). Influence of mild cold on 24 h energy expenditure, resting metabolism and diet-induced thermogenesis. British Journal of Nutrition 45, 257–267.CrossRefGoogle ScholarPubMed
Frayn, K.N., Whyte, P.L., Benson, H.A., Earl, D.J. & Smith, H.A. (1989). Changes in forearm blood flow at elevated ambient temperatures and their role in the apparent impairment of glucose tolerance. Clinical Science 76, 323–328.CrossRefGoogle ScholarPubMed
Ismail-Beigi, G. & Edelman, I.S. (1970). Mechanism of thyroid calorigenesis: role of active sodium transport. Proceedings of the National Academy of Sciences, USA 67, 1071–1078.CrossRefGoogle Scholar
James, W.P.T. & Trayhurn, P. (1976). An integrated view of the metabolic and genetic basis for obesity. Lancet ii, 770–773.CrossRefGoogle Scholar
Ng, L.L., Bruce, M.A. & Hockaday, T.D.R. (1987). Leucocyte sodium pump activity after meals or insulin in normal and obese subjects: cause for increased energetic efficiency in obesity? British Medical Journal 295, 1369–1373.CrossRefGoogle ScholarPubMed
Ng, L.L. & Hockaday, T.D.R. (1986). Non-esterified fatty acids may regulate human lcucocyte sodium pump activity. Clinical Science 71, 737–742.CrossRefGoogle ScholarPubMed
Ng, L.L. & Hockaday, T.D.R. (1987). The effect of oral glucose on the leucocyte sodium pump in normal and obese subjects. Clinical Endocrinology 27, 345–353.CrossRefGoogle ScholarPubMed
Ng, L.L. & Hockaday, T.D.R. (1988). The effect of Intralipid infusion on the human leucocyte sodium pump in vivo. British Journal of Nutrition 60, 49–55.CrossRefGoogle ScholarPubMed
Rosic, N.K., Standaert, M.L. & Pollet, R.J. (1985). The mechanism of insulin stimulation of (Na+, K+)-ATPase transport activity in muscle. Journal of Biological Chemistry 260, 6206–6212.CrossRefGoogle ScholarPubMed
Simon, G. (1989). Is intracellular sodium increased in hypertension? Clinical Science 76, 455–461.CrossRefGoogle ScholarPubMed
Shimizu, S., Inoue, K., Tani, Y. & Yamada, H. (1979). Enzymatic microdetermination of serum free fatty acids. Analytical Biochemistry 98, 341–345.CrossRefGoogle ScholarPubMed
Swaminathan, R., Burrows, G. & McMurray, J. (1982). Energy cost of sodium pump activity in man: an in-vivo study of metabolic rate in human subjects given digoxin. IRCS Clinical Biochemistry 10, 949Google Scholar
Turaihi, K., Baron, D.N. & Dandona, P. (1987). Increased leucocyte Na-K ATPase in obesity: reversal following weight loss. Metabolism 36, 851–855.CrossRefGoogle ScholarPubMed
Turaihi, K., Baron, D.N. & Dandona, P. (1988 a). Effect of glucose intake on 86Rb influx and [3H]ouabain binding. Metabolism 37, 171–174.CrossRefGoogle ScholarPubMed
Turaihi, K., D'Souza, V., Wakeling, A. & Dandona, P. (1988 b). Diminished [3H]ouabain binding and 86Rb influx by leucocytes in anorexia nervosa. Metabolism 37, 486–490.CrossRefGoogle ScholarPubMed