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The effect of dietary zinc depletion and repletion on rats: Zn concentration in various tissues and activity of pancreatic γ-glutamyl hydrolase (EC 3.4.22.12) as indices of Zn status

  • Mary C. Canton (a1) and F. M. Cremin (a1)

Abstract

Unlike severe zinc deficiency, marginal Zn deficiency is difficult to identify in rats because no reliable indicator of suboptimal Zn status is currently available. We have previously observed reduced pancreatic γ-glutamyl hydrolase (EC 3.4.22.12) activity and impaired pteroylpolyglutamate absorption in Zn-deficient rats. In the present study the effect of Zn depletion and repletion on the Zn concentration of various tissues and on the activity of this enzyme was investigated. The objective was to determine the sensitivity of these variables to Zn depletion and to evaluate their usefulness as indices of Zn status. Male Wistar rats (about 180 g), maintained from weanling on a purified Zn-adequate diet, were randomly allocated into twelve groups. A pretreatment control group was killed immediately. The remaining eleven groups were fed on a Zn-deficient diet and a group killed daily for 7 d (Zn-depleted groups). The remaining four groups were re-fed the Zn-adequate diet and a group killed daily (Zn-repleted groups). On analysis, pancreas and spleen Zn levels responded most rapidly to reduced Zn intake, followed by tibia, liver, kidney and plasma. Zn concentration was maintained in testes. Reduced plasma folate levels were also observed. A significant reduction in pancreatic γ-glutamyl hydrolase activity before the depletion of many tissue Zn stores confirms the Zn sensitivity of the enzyme. It was concluded that future investigation into the inter-relationship between Zn and folate metabolism may be useful in identifying a sensitive, biochemical index of Zn status.

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References

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Brown, E. D., Chan, W. & Smith, J. C. (1978). Bone mineralization during a developing zinc deficiency. Proceedings of the Society for Experimental Biology and Medicine 157, 211214.
Burch, R. E., Hahn, H. K. J. & Sullivan, J. F. (1975). Newer aspects of the roles of zinc, manganese and copper in human nutrition. Clinical Chemistry 21, 501520.
Butterworth, C. E., Baugh, C. M. & Krumdieck, C. L. (1969). A study of folate absorption and metabolism in man utilizing carbon-14-labelled polyglutamates synthesized by the solid phase method. Journal of Clinical Investigation 48, 11311142.
Butterworth, C. E., Santini, R. & Frommeyer, W. B. (1963). The pteroylglutamate components of American diets as determined by chromatographic fractionation. Journal of Clinical Investigation 4, 19291939.
Canton, M. C., Cotter, B. M., Cremin, F. M. & Morrissey, P. A. (1989). The effect of dietary zinc deficiency on pancreatic γ-glutamyl hydrolase (EC 3.4.22.12) activity and on the absorption of pteroylpolyglutamate in rats. British Journal of Nutrition 62, 185193.
Chandler, C. J., Wang, T. T. Y. & Halsted, C. H. (1986). Pteroylpolyglutamate hydrolase from human jejunal brush borders. Journal of Biological Chemistry 261, 928933.
Chesters, J. K. & Will, M. (1978). The assessment of zinc status of an animal from the uptake of 65Zn by the cells of whole blood in vitro. British Journal of Nutrition 39, 297306.
Crofton, R. W., Clapham, M., Humphries, W. R., Aggett, P. J. & Mills, C. F. (1983). Leucocyte and tissue zinc concentrations in the growing pig. Proceedings of the Nutrition Society 42, 128A.
Darcy-Vrillon, B., Selhub, J. & Rosenberg, I. H. (1988). Analysis of sequential events in intestinal absorption of folylpolyglutamate. American Journal of Physiology 255, G361G366.
Fuller, N. J., Evans, P. H., Howlett, M. & Bates, C. J. (1988). The effects of dietary folate and zinc on the outcome of pregnancy and early growth in rats. British Journal of Nutrition 59, 251259.
Giugliano, R. & Millward, D. J. (1984). Growth and zinc homeostasis in the severely Zn-deficient rat. British Journal of Nutrition 52, 545560.
Hambidge, K. M. (1988). Assessing the trace element status of man. Proceedings of the Nutrition Society 47, 3744.
Hepner, G. W. (1969). The absorption of pteroylglutamic (folic) acid in rats. British Journal of Haematology 16, 241249.
Hove, E., Elvehjem, C. A. & Hart, E. B. (1938). Further studies on zinc deficiency in rats. American Journal of Physiology 124, 750758.
Jagerstad, M. & Westesson, A.-K. (1974). The hydrolysis of pteroylpolyglutamates in the small intestine. Scandinavian Journal of Gastroenterology 9, 639643.
Keen, C. L., Golub, M. S., Gershwin, M. E., Lonnerdal, B. & Hurley, L. S. (1988). Studies of marginal zinc deprivation in rhesus monkeys. III. Use of liver biopsy in the assessment of zinc status. American Journal of Clinical Nutrition 47, 10411045.
Keen, C. L., Reinstein, N. H., Goudey-Lefevre, J., Lefevre, M., Lonnerdal, B., Schneeman, B. O.& Hurley, L. S. (1985). Effect of dietary copper and zinc levels on tissue copper, zinc and iron in male rats. Biological Trace Element Research 8, 123136.
Kesavan, V. & Noronha, J. M. (1983). Folate malabsorption in aged rats related to low levels of pancreatic folyl conjugase. American Journal of Clinical Nutrition 37, 262267.
King, J. C. (1986). Assessment of techniques for determining human zinc requirements. Journal of the American Dietetic Association 86, 15231528.
Kramer, T. R. (1984). Reevaluation of zinc deficiency on concanavalin-A-induced rat spleen lymphocyte proliferation. Journal of Nutrition 114, 953963.
Law, J. S., McBride, S. A., Graham, S., Nelson, N. R., Slotnick, B. M. & Henkin, R. I. (1988). Zinc deficiency decreases the activity of calmodulin regulated cyclic nucleotide phosphodiesterases in vivo in selected rat tissues. Biological Trace Element Research 16, 221226.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265275.
Mathur, A., Wallenius, K. & Abdulla, M. (1978). Assessment of sub-clinical zinc-deficiency for carcinogenetic and other long-term studies in rats. Archives of Oral Biology 23, 10771082.
Mills, C. F. (1987). Biochemical and physiological indicators of mineral status in animals: copper, cobalt and zinc. Journal of Animal Science 65, 17021711.
Mills, C. F., Quarterman, J., Williams, R. B., Dalgarno, A. C. & Panic, B. (1967). The effects of zinc deficiency on pancreatic carboxypeptidase activity and protein digestion and absorption in the rat. Biochemical Journal 102, 712718.
Milne, D. B., Ralston, N. V. C. & Wallwork, J. C. (1985). Zinc content of blood cellular components and lymph node and spleen lymphocytes in severely zinc-deficient rats. Journal of Nutrition 115, 10731078.
Momcilovic, B., Belonje, B. & Shah, B. G. (1975). Suitability of young rat tissue for a zinc bioassay. Nutrition Reports International 11, 445452.
Ott, E. A., Smith, W. H., Stob, M. & Beeson, W. M. (1964). Zinc deficiency syndrome in the young lamb. Journal of Nutrition 82, 4150.
Prasad, A. S., Oberleas, D., Wolf, P. & Horwitz, J. P (1967). Studies on zinc deficiency: changes in trace elements and enzyme activities in tissues of zinc-deficient rats. Journal of Clinical Investigation 46, 549557.
Roth, H.-P. & Kirchgessner, M. (1980). Zinc metalloenzyme activities. World Review of Nutrition and Dietetics 34, 144160.
Roth, H.-P. & Kirchgessner, M. (1981). Zinc and insulin metabolism. Biological Trace Element Research 3, 1332.
Sato, M., Mehra, R. K. & Bremner, I. (1984). Measurement of plasma metallothionein–I in the assessment of the zinc status of zinc-deficient and stressed rats. Journal of Nutrition 114, 16831689.
Scott, J. M., Ghanta, V. & Herbert, V. (1974). Trouble-free microbiologic serum and red cell folate assays. American Journal of Medical Technology 40, 125134.
Solomons, N. W. (1979). On the assessment of zinc and copper nutriture in man. American Journal of Clinical Nutrition 32, 856871.
Southon, S., Fairweather-Tait, S. J. & Williams, C. M. (1988). Fetal growth, glucose tolerance and plasma insulin concentration in rats given a marginal-zinc diet in the latter stages of pregnancy. British Journal of Nutrition 59, 315322.
Tamura, T., Kaiser, L. L., Watson, J. E., Halsted, C. H., Hurley, L. S. & Stokstad, E. L. R. (1987). Increased methionine synthetase activity in zinc-deficient rat liver. Archives of Biochemistry and Biophysics 256, 311316.
Tamura, T., Shane, B., Baer, M. T., King, J. C., Margen, S. & Stokstad, E. L. R. (1978). Absorption of mono-and polyglutamyl folates in zinc-depleted man. American Journal of Clinical Nutrition 31, 19841987.
Taylor, C. G., Bettger, W. J. & Bray, T. M. (1988). Effect of dietary zinc or copper deficiency on the primary free radical defense system in rats. Journal of Nutrition 118, 613621.
Wilkins, P. J., Grey, P. C. & Dreosti, I. E. (1972). Plasma zinc as an indicator of zinc status in rats. British Journal of Nutrition 27, 113120.
Williams, R. B. & Mills, C. F. (1970). The experimental production of zinc deficiency in the rat. British Journal of Nutrition 24, 9891003.
Wilson, S. D. & Horne, D. W. (1982). Use of glycerolcryoprotected Lactobacillus casei for microbiological assay of folic acid. Clinical Chemistry 28, 11981200.

Keywords

The effect of dietary zinc depletion and repletion on rats: Zn concentration in various tissues and activity of pancreatic γ-glutamyl hydrolase (EC 3.4.22.12) as indices of Zn status

  • Mary C. Canton (a1) and F. M. Cremin (a1)

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