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The effect of dietary protein deprivation on protein synthesis in the isolated liver parenchymal cell

Published online by Cambridge University Press:  09 March 2007

Anne G. Grant  and
R. Hoffenberg
Anne G. Grant
Affiliation:
Department of Clinical Investigation, MRC Clinical Research Centre, Harrow, Middlesex
R. Hoffenberg
Affiliation:
Department of Clinical Investigation, MRC Clinical Research Centre, Harrow, Middlesex
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Abstract

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1. Parenchymal cells were isolated from the liver of rats that had been deprived of dietary protein for 3 weeks.

2. The cells were two-thirds the diameter of those derived from livers of normal animals and consumed oxygen at a rate of 16.2±2.7 μl/h per 106 viable cells, half the normal value.

3. Albumin and transferrin were synthesized at rates of 0.94±0.12 and 0.60±0.07 μg/h per 106 viable cells respectively and urea at a rate of 0.77±0.12 μg/h per 106 cells. This represents a 25-50% decrease in the rates of synthesis measured in cells isolated from normal livers.

4. The results are discussed in relation to the long-term effects of malnutrition on liver cell function.

Type
Research Article
Information
British Journal of Nutrition , Volume 38 , Issue 2 , September 1977 , pp. 255 - 260
Copyright
Copyright © The Nutrition Society 1977

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