Skip to main content Accessibility help
×
Home

Dietary zinc deficiency lowers the proportions of splenic CD90+ (Thy-1+) B-cells and late thymic emigrant T-cells in growing rats

  • Heather J. Hosea (a1), Edward S. Rector (a2) and Carla G. Taylor (a1)

Abstract

Zn-deficient (ZD) rats have a lower proportion of splenic CD90+T-cells which could be due to fewer new T-cells exiting the thymus, defective post-thymic maturation or increased cell death. Post-thymic maturation of splenic lymphocytes and their viability were determined by flow cytometry in weanling rats assigned to ZD ( < 1 mg Zn/kg; ad libitum), diet-restricted (DR; 30 mg Zn/kg; limited to the amount of feed as consumed by ZD rats), marginally Zn-deficient (MZD; 10 mg Zn/kg; ad libitum) or control (30 mg Zn/kg; ad libitum) groups for 3 weeks. ZD rats had a 29 % lower percentage of splenic CD90+T-cells and both ZD and DR rats had a 30 % lower proportion of splenic CD90+B-cells compared with control rats. When the splenic CD90+T-cells were characterised further, there was no difference among the groups in the first two stages of post-thymic development; however, ZD, DR and MZD rats had a 42 % lower proportion of late thymic emigrants (TCRαβ+CD90+CD45RC+RT6.1+) compared with control rats. There was no difference among groups in the proportion of splenic CD90+T-cells in the non-viable region; however, ZD rats had a higher proportion of CD90+B-cells in the non-viable region compared with MZD and control animals, suggesting that this phenotype was more susceptible to cell death during deficiency. The lower proportion of splenic CD90+T-cells in ZD rats does not appear to be due to a defect in thymic production or increased cell death in the spleen. Future studies should determine if late thymic emigrants have homed to other peripheral organs.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Dietary zinc deficiency lowers the proportions of splenic CD90+ (Thy-1+) B-cells and late thymic emigrant T-cells in growing rats
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Dietary zinc deficiency lowers the proportions of splenic CD90+ (Thy-1+) B-cells and late thymic emigrant T-cells in growing rats
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Dietary zinc deficiency lowers the proportions of splenic CD90+ (Thy-1+) B-cells and late thymic emigrant T-cells in growing rats
      Available formats
      ×

Copyright

Corresponding author

*Corresponding author: Dr Carla G. Taylor, fax +1 204 474 7593, email ctaylor@cc.umanitoba.ca

References

Hide All
1Hosea, HJ, Rector, ES & Taylor, CG (2003) Zinc-deficient rats have fewer recent thymic emigrant (CD90+) T lymphocytes in spleen and blood. J Nutr 133, 42394242.
2Hosseinzadeh, H & Goldschneider, I (1993) Recent thymic emigrants in the rat express a unique antigenic phenotype and undergo post-thymic maturation in peripheral lymphoid tissues. J Immunol 150, 16701679.
3Kampinga, J, Groen, H, Klatter, FA, Pater, JM, van Petersen, AS, Roser, B, Nieuwenhuis, P & Aspinall, R (1997) Post-thymic T-cell development in the rat. Thymus 24, 173200.
4Iwakoshi, NN, Goldschneider, I, Tausche, F, Mordes, JP, Rossini, AA & Greiner, DL (1998) High frequency apoptosis of recent thymic emigrants in the liver of lymphopenic diabetes-prone BioBreeding rats. J Immunol 160, 58385850.
5Darzynkiewicz, Z, Bedner, E & Smolewski, P (2001) Flow cytometry in analysis of cell cycle and apoptosis. Semin Hematol 38, 179193.
6Hosea, HJ, Rector, ES & Taylor, CG (2004) Dietary repletion can replenish reduced T cell subset numbers and lymphoid organ weight in zinc-deficient and energy-restricted rats. Br J Nutr 91, 741747.
7King, LE & Fraker, PJ (2002) Zinc deficiency in mice alters myelopoiesis and hematopoiesis. J Nutr 132, 33013307.
8King, LE, Osati-Ashtiani, F & Fraker, PJ (2002) Apoptosis plays a distinct role in the loss of precursor lymphocytes during zinc deficiency in mice. J Nutr 132, 974979.
9La Gruta, NL, Driel, IR & Gleeson, PA (2000) Peripheral T cell expansion in lymphopenic mice results in a restricted T cell repertoire. Eur J Immunol 30, 33803386.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed