Skip to main content Accessibility help
×
Home

Dietary supplemental vitamin D3 enhances phosphorus absorption and utilisation by regulating gene expression of related phosphate transporters in the small intestine of broilers

  • Yuxin Shao (a1), Qian Wen (a1), Shumin Zhang (a1), Lin Lu (a1), Liyang Zhang (a1), Xiudong Liao (a1) and Xugang Luo (a1)...

Abstract

The present study was carried out to evaluate the effect of dietary supplemental vitamin D3 (VD3) on P absorption and utilisation as well as its related mechanisms in the small intestine of broilers. A total of 384 1-d-old Arbor Acres male broilers were assigned randomly into four treatments following a completely randomised design with a 2 (dietary non-phytate P (NPP) contents: 0·43 and 0·22 %)×2 (dietary VD3 supplemental levels: 0 and 87·5 μg/kg) factorial arrangement. The experiment lasted for 22 d. The results showed that P contents in serum from the hepatic portal vein and tibia ash of broilers were higher (P<0·05) for 0·43 % NPP than for 0·22 % NPP. The type IIb Na-dependent phosphate cotransporter (NaP-IIb) protein expressions in the duodenum and ileum were higher (P<0·05) also for 0·43 % NPP than 0·22 % NPP. Supplementation of VD3 enhanced (P<0·05) tibia P retention rate and type III Na-dependent phosphate cotransporter (PiT)-1 protein expression in the duodenum of all broilers. Moreover, VD3 supplementation decreased (P<0·002) mortality and increased (P<0·02) serum P content from the hepatic portal vein after 4 h of feeding, tibia ash content, tibia ash P content and protein expressions of NaP-IIb and PiT-1 in the jejunum of broilers fed diet with 0·22 % NPP. Thus, dietary supplemental VD3 promoted intestinal P absorption and bone P utilisation, and this effect might be associated with enhanced PiT-1 levels in the duodenum and PiT-1 and NaP-IIb levels in the jejunum respectively when dietary NPP is limiting.

Copyright

Corresponding author

*Corresponding authors: X. Liao, email liaoxd56@163.com; X. Luo, fax +86 10 62810184, email wlysz@263.net

Footnotes

Hide All

These authors contributed equally to this work.

Footnotes

References

Hide All
1. Berndt, T & Kumar, R (2009) Novel mechanisms in the regulation of phosphorus homeostasis. Physiology (Bethesda) 24, 1725.
2. Webster, AB (2004) Welfare implications of avian osteoporosis. Poult Sci 83, 184192.
3. Long, PH, Lee, SR, Rowland, GN, et al. (1984) Experimental rickets in broilers: gross, microscopic, and radiographic lesions. II. Calcium deficiency. Avian Dis 28, 921932.
4. National Research Council (1994) Nutrient Requirements of Poultry, 9th ed. Washington, DC: National Academies Press.
5. Liu, SB, Li, SF, Lu, L, et al. (2012) Estimation of standardized phosphorus retention for corn, soybean meal, and corn–soybean meal diet in broilers. Poult Sci 91, 18791885.
6. Liu, SB, Xie, JJ, Lu, L, et al. (2013) Estimation of standardized phosphorus retention for inorganic phosphate sources in broilers. J Anim Sci 91, 37663771.
7. Chen, TC, Castillo, L, Korycka-Dahl, M, et al. (1974) Role of vitamin D metabolites in phosphate transport of rat intestine. J Nutr 104, 10561060.
8. Holick, MF, Binkley, NC, Bischoff-Ferrari, HA, et al. (2011) Evaluation, treatment, and prevention of vitamin D deficiency: an endocrine society clinical practice guideline. J Clin Endocrinol Metab 96, 19111930.
9. Peterlik, M & Wasserman, RH (1978) Effect of vitamin D on transepithelial phosphate transport in chick intestine. Am J Physiol 234, 379388.
10. Mohammed, A, Gibney, MJ & Taylor, TG (1991) The effects of dietary levels of inorganic phosphorus, calcium and cholecalciferol on the digestibility of phytate-P by the chick. Br J Nutr 66, 251259.
11. Edwards, HM Jr (1993) Dietary 1,25-dihydroxycholecalciferol supplementation increases natural phytate phosphorus utilization in chickens. J Nutr 123, 567577.
12. Fritts, CA & Waldroup, PW (2003) Effect of source and level of vitamin D on live performance and bone development in growing broilers. J Appl Poultry Res 12, 2545.
13. Rama Rao, SV, Mvln, R & Reddy, MR (2007) Performance of broiler chicks fed high levels of cholecalciferol in diets containing sub-optimal levels of calcium and non-phytate phosphorus. Anim Feed Sci Technol 134, 7788.
14. Furlong, RF (2005) Insights into vertebrate evolution from the chicken genome sequence. Genome Biol 6, 207.
15. Werner, A & Kinne, RK (2001) Evolution of the Na–P(i) cotransport systems. Am J Physiol Regul Integr Comp Physiol 280, 301312.
16. Murer, H, Forster, I & Biber, J (2004) The sodium phosphate cotransporter family SLC34. Pflugers Arch 447, 763767.
17. Liao, XD, Suo, HQ, Lu, L, et al. (2017) Effects of sodium, 1,25-dihydroxyvitamin D3 and parathyroid hormone fragment on inorganic P absorption and type IIb sodium–phosphate cotransporter expression in ligated duodenal loops of broilers. Poult Sci 96, 23442350.
18. Marks, J, Srai, SK, Biber, J, et al. (2006) Intestinal phosphate absorption and the effect of vitamin D: a comparison of rats with mice. Exp Physiol 91, 531537.
19. Collins, JF, Bai, L & Ghishan, FK (2004) The SLC20 family of proteins: dual functions as sodium–phosphate cotransporters and viral receptors. Pflugers Arch 447, 647652.
20. Zoidis, E, Ghirlanda-Keller, C, Gosteli-Peter, M, et al. (2004) Regulation of phosphate (Pi) transport and NaPi-III transporter (Pit-1) mRNA in rat osteoblasts. J Endocrinol 181, 531540.
21. Suzuki, A, Ghayor, C, Guicheux, J, et al. (2006) Enhanced expression of the inorganic phosphate transporter Pit-1 is involved in BMP-2-induced matrix mineralization in osteoblast-like cells. J Bone Miner Res 21, 674683.
22. Tatsumi, S, Segawa, H, Morita, K, et al. (1998) Molecular cloning and hormonal regulation of PiT-1, a sodium-dependent phosphate cotransporter from rat parathyroid glands. Endocrinology 139, 16921699.
23. Katai, K, Miyamoto, K, Kishida, S, et al. (1999) Regulation of intestinal Na+-dependent phosphate co-transporters by a low-phosphate diet and 1,25-dihydroxyvitamin D3 . Biochem J 343, 705712.
24. Kilkenny, C, Browne, WJ, Cuthill, IC, et al. (2010) Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research. Plos Biol 8, e1000412.
25. Hu, YX, Liao, XD, Wen, Q, et al. (2018) Phosphorus absorption and gene expressions of related transporters in the small intestine of broilers. Br J Nutr 119, 13461354.
26. Thiex, NJ, Manson, H, Andersson, S, et al. (2002) Determination of crude protein in animal feed, forage, grain, and oilseeds by using block digestion with a copper catalyst and steam distillation into boric acid: collaborative study. J AOAC Int 85, 309317.
27. Li, S, Lin, Y, Lu, L, et al. (2011) An estimation of the manganese requirement for broilers from 1 to 21 d of age. Biol Trace Elem Res 143, 939948.
28. Huang, YL, Lu, L, Xie, JJ, et al. (2013) Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed diets with low or high phytate content. Anim Feed Sci Technol 179, 144148.
29. Davies, JL, Andrews, GS, Miller, R, et al. (1973) Comparison of the stannous chloride and vanadate methods for estimation of serum inorganic phosphorus by use of the “SMA12-60”. Clin Chem 19, 411414.
30. Rutherfurd, SM, Chung, TK, Morel, PC, et al. (2004) Effect of microbial phytase on ileal digestibility of phytate phosphorus, total phosphorus, and amino acids in a low-phosphorus diet for broilers. Poult Sci 83, 6168.
31. Leytem, AB, Kwanyuen, P & Thacker, P (2008) Nutrient excretion, phosphorus characterization, and phosphorus solubility in excreta from broiler chicks fed diets containing graded levels of wheat distillers grains with solubles. Poult Sci 87, 25052511.
32. Goldenberg, H & Fernandez, A (1966) Simplified method for the estimation of inorganic phosphorus in body fluids. Clin Chem 12, 871882.
33. Livak, KJ & Schmittgen, TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(−Delta Delta C) method. Methods 25, 402408.
34. Chow, S-C, Wang, H & Shao, J (2007) Sample Size Calculations in Clinical Research, 2nd ed. Boca Raton, FL: Chapman & Hall/CRC Press.
35. Steel, RGD, Torrie, JH & Dickey, DA (1997) Principles and Procedures of Statistics: A Biometrical Approach, 3rd ed. New York: McGraw Hill.
36. Li, J, Yuan, J, Guo, Y, et al. (2012) The influence of dietary calcium and phosphorus imbalance on intestinal NaPi-IIb and calbindin mRNA expression and tibia parameters of broilers. Asian-Australas J Anim Sci 25, 552558.
37. Nie, W, Yang, Y, Yuan, J, et al. (2013) Effect of dietary nonphytate phosphorus on laying performance and small intestinal epithelial phosphate transporter expression in Dwarf pink-shell laying hens. J Anim Sci Biotechnol 4, 34.
38. Hamdi, M, Lopez-Verge, S, Manzanilla, EG, et al. (2015) Effect of different levels of calcium and phosphorus and their interaction on the performance of young broilers. Poult Sci 94, 21442151.
39. Edwards, HM Jr (2002) Studies on the efficacy of cholecalciferol and derivatives for stimulating phytate utilization in broilers. Poult Sci 81, 10261031.
40. Chun, S, Bamba, T, Suyama, T, et al. (2016) A high phosphorus diet affects lipid metabolism in rat liver: a DNA microarray analysis. PLOS ONE 11, e0155386.
41. Gardiner, EE (1962) The relationship between dietary phosphorus level and the level of plasma inorganic phosphorus of chicks. Poult Sci 41, 11561163.
42. Abudabos, AM (2012) Optimal dietary phosphorus for broiler performance, bone integrity and reduction of phosphorus excretion. Asian J Anim Vet Adv 7, 288298.
43. Matsumoto, T, Fontaine, O & Rasmussen, H (1980) Effect of 1,25-dihydroxyvitamin D-3 on phosphate uptake into chick intestinal brush border membrane vesicles. Biochim Biophys Acta 599, 1323.
44. Lee, DB, Walling, MW & Corry, DB (1986) Phosphorus transport across rat jejunum: influence of sodium, pH, and 1,25-(OH)2D3 . Am J Physiol 250, G369G373.
45. Han, JC, Yang, XD, Zhang, T, et al. (2009) Effects of 1alpha-hydroxycholecalciferol on growth performance, parameters of tibia and plasma, meat quality, and type IIb sodium phosphate cotransporter gene expression of one- to twenty-one-day-old broilers. Poult Sci 88, 323329.
46. Birger, S (2017) Function of the digestive system. J Appl Poult Res 23, 306314.
47. Veum, TL (2010) Phosphorus and calcium nutrition and metabolism. In Phosphorus and Calcium Utilization and Requirements in Farm Animals, pp. 94111 [DMSS Vitti and E Kebreab, editors]. Oxfordshire: CAB International.
48. Nelson, TS (1967) The utilization of phytate phosphorus by poultry – a review. Poult Sci 46, 862871.
49. Biehl, RR & Baker, DH (1997) 1Alpha-hydroxycholecalciferol does not increase the specific activity of intestinal phytase but does improve phosphorus utilization in both cecectomized and sham-operated chicks fed cholecalciferol-adequate diets. J Nutr 127, 20542059.
50. Biehl, RR & Baker, DH (1997) Utilization of phytate and nonphytate phosphorus in chicks as affected by source and amount of vitamin D3 . J Anim Sci 75, 29862993.
51. Snow, JL, Baker, DH & Parsons, CM (2004) Phytase, citric acid, and 1alpha-hydroxycholecalciferol improve phytate phosphorus utilization in chicks fed a corn–soybean meal diet. Poult Sci 83, 11871192.
52. Onyango, EM, Asem, EK & Adeola, O (2006) Dietary cholecalciferol and phosphorus influence intestinal mucosa phytase activity in broiler chicks. Br Poult Sci 47, 632639.
53. Hilfiker, H, Hattenhauer, O, Traebert, M, et al. (1998) Characterization of a murine type II sodium–phosphate cotransporter expressed in mammalian small intestine. Proc Natl Acad Sci U S A 95, 1456414569.
54. Liu, SB, Hu, YX, Liao, XD, et al. (2016) Kinetics of phosphorus absorption in ligated small intestinal segments of broilers. J Anim Sci 94, 33123320.
55. Radanovic, T, Wagner, CA, Murer, H, et al. (2005) Regulation of intestinal phosphate transport. I. Segmental expression and adaptation to low-P(i) diet of the type IIb Na(+)–P(i) cotransporter in mouse small intestine. Am J Physiol Gastrointest Liver Physiol 288, 496500.
56. Saddoris, KL, Fleet, JC & Radcliffe, JS (2010) Sodium-dependent phosphate uptake in the jejunum is post-transcriptionally regulated in pigs fed a low-phosphorus diet and is independent of dietary calcium concentration. J Nutr 140, 731736.
57. Hattenhauer, O, Traebert, M, Murer, H, et al. (1999) Regulation of small intestinal Na–Pi type IIb cotransporter by dietary phosphate intake. Am J Physiol Gastrointest Liver Physiol 277, G756G762.
58. Biber, J, Custer, M, Magagnin, S, et al. (1996) Renal Na/Pi-contransporters. Kidney Int 49, 981985.
59. Boyer, CJ, Baines, AD, Beaulieu, E, et al. (1998) Immunodetection of a type III sodium-dependent phosphate cotransporter in tissues and OK cells. Biochim Biophys Acta 1368, 7383.
60. Custer, M, Spindler, B, Verrey, F, et al. (1997) Identification of a new gene product (diphor-1) regulated by dietary phosphate. Am J Physiol 273, 801806.
61. Liu, Y, Beyer, A & Aebersold, R (2016) On the dependency of cellular protein levels on mRNA abundance. Cell 165, 535550.
62. Yan, F, Angel, R & Ashwell, CM (2007) Characterization of the chicken small intestine type IIb sodium phosphate cotransporter. Poult Sci 86, 6776.
63. Bai, L, Collins, JF & Ghishan, FK (2000) Cloning and characterization of a type III Na-dependent phosphate cotransporter from mouse intestine. Am J Physiol Cell Physiol 279, 11351143.

Keywords

Dietary supplemental vitamin D3 enhances phosphorus absorption and utilisation by regulating gene expression of related phosphate transporters in the small intestine of broilers

  • Yuxin Shao (a1), Qian Wen (a1), Shumin Zhang (a1), Lin Lu (a1), Liyang Zhang (a1), Xiudong Liao (a1) and Xugang Luo (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed