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Dietary iron concentration influences serum concentrations of manganese in rats consuming organic or inorganic sources of manganese

  • Huaiyong Zhang (a1), Elizabeth R. Gilbert (a2), Shuqin Pan (a1), Keying Zhang (a1), Xuemei Ding (a1), Jianping Wang (a1), Qiufeng Zeng (a1) and Shiping Bai (a1)...

Abstract

To determine the effects of dietary Fe concentration on Mn bioavailability in rats fed inorganic or organic Mn sources, fifty-four 22-d-old male rats were randomly assigned and fed a basal diet (2·63 mg Fe/kg) supplemented with 0 (low Fe (L-Fe)), 35 (adequate Fe (A-Fe)) or 175 (high Fe (H-Fe)) mg Fe/kg with 10 mg Mn/kg from MnSO4 or Mn–lysine chelate (MnLys). Tissues were harvested after 21 d of feeding. Serum Mn was greater (P<0·05) in MnLys rats than in MnSO4 rats, and in L-Fe rats than in A-Fe or H-Fe rats. Duodenal divalent metal transporter-1 (DMT1) mRNA was lower (P<0·05) in H-Fe rats than in A-Fe rats for the MnSO4 treatment; however, no significant difference was observed between them for MnLys. Liver DMT1 mRNA abundance was greater (P<0·05) in MnSO4 than in the MnLys group for H-Fe rats. The DMT1 protein in duodenum and liver and ferroportin 1 (FPN1) protein in liver was greater (P<0·05) in the MnSO4 group than in the MnLys group, and in L-Fe rats than in H-Fe rats. Duodenal FPN1 protein was greater (P<0·05) in L-Fe rats than in A-Fe rats for the MnLys treatment, but it was not different between them for the MnSO4 treatment. Results suggest that MnLys increased serum Mn concentration as compared with MnSO4 in rats irrespective of dietary Fe concentration, which was not because of the difference in DMT1 and FPN1 expression in the intestine and liver.

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Corresponding author

* Corresponding author: Dr S. Bai, fax +86 835 2885 630, email shipingbai@sicau.edu.cn

References

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1. Umbreit, J (2005) Iron deficiency: a concise review. Am J Hematol 78, 225231.
2. Siega-Riz, AM, Hartzema, AG, Turnbull, C, et al. (2006) The effects of prophylactic iron given in prenatal supplements on iron status and birth outcomes: a randomized controlled trial. Am J Obstet Gynecol 194, 512519.
3. Pathak, P, Kapil, U, Kapoor, SK, et al. (2004) Prevalence of multiple micronutrient deficiencies amongst pregnant women in a rural area of Haryana. Indian J Pediatr 71, 10071014.
4. Duque, X, Flores-Hernández, S, Flores-Huerta, S, et al. (2006) Prevalence of anemia and deficiency of iron, folic acid, and zinc in children younger than 2 years of age who use the health services provided by the Mexican Social Security Institute. BMC Public Health 7, 345.
5. Knovich, MA, Il’yasova, D, Ivanova, A, et al. (2008) The association between serum copper and anaemia in the adult Second National Health and Nutrition Examination Survey (NHANES II) population. Br J Nutr 99, 12261229.
6. Thompson, K, Molina, R, Donaghey, T, et al. (2006) The influence of high iron diet on rat lung manganese absorption. Toxicol Appl Pharmacol 210, 1723.
7. Davis, CD, Wolf, TL & Greger, JL (1992) Varying levels of manganese and iron affect absorption and gut endogenous losses of manganese by rats. J Nutr 122, 13001308.
8. Hansen, SL, Trakooljul, N, Liu, HC, et al. (2009) Iron transporters are differentially regulated by dietary iron, and modifications are associated with changes in manganese metabolism in young pigs. J Nutr 139, 14741479.
9. Jolliff, JS & Mahan, DC (2012) Effect of dietary inulin and phytase on mineral digestibility and tissue retention in weanling and growing swine. J Anim Sci 90, 30123022.
10. Liu, Y, Ma, YL, Zhao, JM, et al. (2014) Digestibility and retention of zinc, copper, manganese, iron, calcium, and phosphorus in pigs fed diets containing inorganic or organic minerals. J Anim Sci 92, 34073415.
11. Li, X, Xie, J, Lu, L, et al. (2013) Kinetics of manganese transport and gene expressions of manganese transport carriers in Caco-2 cell monolayers. Biometals 26, 941953.
12. Fleming, MD, Romano, MA, Su, MA, et al. (1998) Nramp2 is mutated in the anemic Belgrade (b) rat evidence of a role for Nramp2 in endosomal iron transport. Proc Natl Acad Sci USA 95, 11481153.
13. Gunshin, H, Allerson, CR, Polycarpou-Schwarz, M, et al. (2001) Iron-dependent regulation of the divalent metal ion transporter. FEBS Lett 509, 309316.
14. Thompson, K, Molina, RM, Donaghey, T, et al. (2007) Olfactory uptake of manganese requires DMT1 and is enhanced by anemia. FASEB J 21, 223230.
15. Kim, J, Li, Y, Buckett, PD, et al. (2012) Iron-responsive olfactory uptake of manganese improves motor function deficits associated with iron deficiency. PLOS ONE 7, e33533.
16. Illing, AC, Shawki, A, Cunningham, CL, et al. (2012) Substrate profile and metal-ion selectivity of human divalent metal-ion transporter-1. J Biol Chem 287, 3048530496.
17. Yin, Z, Jiang, H, Lee, ES, et al. (2010) Ferroportin is a manganese-responsive protein that decreases manganese cytotoxicity and accumulation. J Neurochem 112, 11901198.
18. Madejczyk, MS & Ballatori, N (2012) The iron transporter ferroportin can also function as a manganese exporter. Biochim Biophys Acta 1818, 651657.
19. Ashmead, HD (2012) Amino Acid Chelation in Human and Animal Nutrition. Boca Raton, FL: CRC Press.
20. Reeves, PG, Rossow, KL & Lindlauf, J (1993) Development and testing of the AIN-93 purified diets for rodents: results on growth, kidney calcification and bone mineralization in rats and mice. J Nutr 123, 19231931.
21. Bai, SP, Lu, L, Luo, XG, et al. (2008) Kinetics of manganese absorption in ligated small intestinal segments of broilers. Poult Sci 87, 25962604.
22. Bai, SP, Lu, L, Wang, RL, et al. (2012) Manganese source affects manganese transport and gene expression of divalent metal transporter 1 in the small intestine of broilers. Br J Nutr 108, 267276.
23. Brain, JD, Heilig, E, Donaghey, TC, et al. (2006) Effects of iron status on transpulmonary transport and tissue distribution of Mn and Fe. Am J Respir Cell Mol Biol 34, 330337.
24. Livak, KJ & Schmittgen, TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) method. Methods 25, 402408.
25. Urrutia, P, Aguirre, P, Esparza, A, et al. (2013) Inflammation alters the expression of DMT1, FPN1 and hepcidin, and it causes iron accumulation in central nervous system cells. J Neurochem 126, 541549.
26. Kamei, A, Watanabe, Y, Ishijima, T, et al. (2010) Dietary iron-deficient anemia induces a variety of metabolic changes and even apoptosis in rat liver: a DNA microarray study. Physiol Genomics 42, 149156.
27. Azebedo, JL, Willis, WT, Turcotte, LP, et al. (1989) Reciprocal changes of muscle oxidases and liver enzymes with recovery from iron deficiency. Am J Physiol 256, E401E405.
28. Klempa, KL, Willis, WT, Chengson, R, et al. (1989) Iron deficiency decreases gluconeogenesis in isolated rat hepatocytes. J Appl Physiol 67, 18681872.
29. Zoller, H, Theurl, I, Koch, R, et al. (2002) Mechanisms of iron mediated regulation of the duodenal iron transporters divalent metal transporter 1 and ferroportin 1. Blood Cells Mol Dis 29, 488497.
30. Foot, NJ, Dalton, HE, Shearwin-Whyatt, LM, et al. (2008) Regulation of the divalent metal ion transporter DMT1 and iron homeostasis by a ubiquitin-dependent mechanism involving Ndfips and WWP2. Blood 112, 42684275.
31. Nemeth, E, Tuttle, MS, Powelson, J, et al. (2004) Hepcidin regulates cellular iron efflux by binding to ferroportin and inducing its internalization. Science 306, 20902093.
32. De Domenico, I, Ward, DM, Langelier, C, et al. (2007) The molecular mechanism of hepcidin-mediated ferroportin down-regulation. Mol Biol Cell 18, 25692578.
33. Nemeth, E & Ganz, T (2006) Regulation of iron metabolism by hepcidin. Annu Rev Nutr 26, 323342.
34. Abboud, S & Haile, DJ (2000) A novel mammalian iron-regulated protein involved in intracellular iron metabolism. J Biol Chem 275, 1990619912.
35. McKie, AT, Marciani, P, Rolfs, A, et al. (2000) A novel duodenal iron-regulated transporter, IREG1, implicated in the basolateral transfer of iron to the circulation. Mol Cell 5, 299309.
36. Roth, JA & Garrick, MD (2003) Iron interactions and other biological reactions mediating the physiological and toxic actions of manganese. Biochem Pharmacol 66, 113.
37. Hansen, SL, Ashwell, MS, Moeser, AJ, et al. (2010) High dietary iron reduces transporters involved in iron and manganese metabolism and increases intestinal permeability in calves. J Dairy Sci 93, 656665.
38. Ji, F, Luo, XG, Lu, L, et al. (2006) Effects of manganese source and calcium on manganese uptake by in vitro everted gut sacs of broilers’ intestinal segments. Poult Sci 85, 12171225.
39. Davis, CD, Zech, L & Greger, JL (1993) Manganese metabolism in rats: an improved methodology for assessing gut endogenous losses. Proc Soc Exp Biol Med 202, 103108.
40. Malecki, EA, Radzanowski, GM, Radzanowski, TJ, et al. (1996) Biliary manganese excretion in conscious rats is affected by acute and chronic manganese intake but not by dietary fat. J Nutr 126, 489498.
41. Roth, JA (2006) Homeostatic and toxic mechanisms regulating manganese uptake, retention, and elimination. Biol Res 39, 4557.
42. Patricia, C (editor) (1995) Official Methods of Analysis of AOAC International. Arlington, VA: AOAC International.
43. Association of American Feed Control Officials (2001) Official Publication. Atlanta, GA: Association of American Feed Control Official Incorporated Company.
44. Ji, F, Luo, XG, Lu, L, et al. (2006) Effect of manganese source on manganese absorption by the intestine of broilers. Poult Sci 85, 812822.
45. Bai, S, Huang, L, Luo, Y, et al. (2014) Dietary manganese supplementation influences the expression of transporters involved in iron metabolism in chickens. Biol Trace Elem Res 160, 352360.
46. Ashmead, HD (1993) Comparative intestinal absorption and subsequent metabolism of metal amino acid chelates and inorganic metal salts. In The Roles of Amino Acid Chelates in Animal Nutrition, pp. 4774 [HD Ashmead, editor]. Park Ridge, NJ: Noyes Publications.
47. Gao, S, Yin, T, Xu, B, et al. (2014) Amino acid facilitates absorption of copper in the Caco-2 cell culture model. Life Sci 109, 5056.

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