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Comparison of chemical and microbiological methods in the estimation of methionine in cowpea (Vigna unguiculata) seeds

  • I. Marta Evans (a1), J. E. Ford (a2), L. C. Hannah (a3) and D. Boulter (a1)

Extract

1. Meals were prepared from the seeds of fifteen varieties of cowpea (Vigna unguiculata), one of lima bean (Phaseolus lunatus) and one of yam bean (Sphenostylis stenocarpa), and their methionine content was determined by six different methods.

2. Total methionine content was determined by two chemical methods (ion-exchange chromatography and a colorimetric procedure) and by two microbiological methods. The ‘available’ methionine content was determined by microbiological assay with Streptococcus zymogenes.

3. All the different methods for total methionine determination gave similar results, with much the same high extent of precision.

4. The values for ‘available’ methionine content were similar to or marginally higher than the corresponding microbiological assay values for total methionine content. There was no indication that the methionine in any of the test samples was not completely available.

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References

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Boyne, A. W., Ford, J. E., Hewitt, D. & Shrimpton, D. H. (1975). Br. J. Nutr. 34, 153.
Ellinger, G. M. & Palmer, R. (1969). Proc. Nutr. Soc. 28, 42A.
Evans, I. M. & Boulter, D. (1974e). J. Sci. Fd Agric. 25, 311.
Evans, I. M. & Boulter, D. (19746). J. Sci. Fd Agric. 25, 919.
Ford, J. E. (1962). Br. J. Nutr. 16, 409.
Gehrke, C. W. & Neuner, T. E. (1974). J. Ass. off. Analyt. Chem. 57, 682.
Njaa, L. R. (1962). Acta chem. scand. 16, 1359.

Comparison of chemical and microbiological methods in the estimation of methionine in cowpea (Vigna unguiculata) seeds

  • I. Marta Evans (a1), J. E. Ford (a2), L. C. Hannah (a3) and D. Boulter (a1)

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