Skip to main content Accessibility help
×
Home

Chronic caffeine intake decreases circulating catecholamines and prevents diet-induced insulin resistance and hypertension in rats

  • Silvia V. Conde (a1), Tiago Nunes da Silva (a1) (a2), Constancio Gonzalez (a3) (a4) (a5), Miguel Mota Carmo (a2), Emilia C. Monteiro (a1) and Maria P. Guarino (a2)...

Abstract

We tested the hypothesis that long-term caffeine intake prevents the development of insulin resistance and hypertension in two pathological animal models: the high-fat (HF) and the high-sucrose (HSu) diet rat. We used six groups of animals: control; caffeine-treated (Caff; 1 g/l in drinking water during 15 d); HF; caffeine-treated HF (HFCaff); HSu; caffeine-treated HSu (HSuCaff). Insulin sensitivity was assessed using the insulin tolerance test. Blood pressure, weight gain, visceral fat, hepatic glutathione, plasma caffeine, insulin and NO, and serum NEFA and catecholamines were measured. Caffeine reversed insulin resistance and hypertension induced by both the HF and HSu diets. In the HF-fed animals caffeine treatment restored fasting insulin levels to control values and reversed increased weight gain and visceral fat mass. In the HSu group, caffeine reversed fasting hyperglycaemia and restored NEFA to control values. There were no changes either in plasma NO or in hepatic glutathione levels. In contrast, caffeine totally prevented the increase in serum catecholamines induced by HF and HSu diets. To test the hypothesis that inhibition of the sympathetic nervous system prevents the development of diet-induced insulin resistance we administered carvedilol, an antagonist of β1, β2 and also α1 adrenoceptors, to HF and HSu rats. Carvedilol treatment fully prevented diet-induced insulin resistance and hypertension, mimicking the effect of caffeine. We concluded that long-term caffeine intake prevented the development of insulin resistance and hypertension in HF and HSu models and that this effect was related to a decrease in circulating catecholamines.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Chronic caffeine intake decreases circulating catecholamines and prevents diet-induced insulin resistance and hypertension in rats
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Chronic caffeine intake decreases circulating catecholamines and prevents diet-induced insulin resistance and hypertension in rats
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Chronic caffeine intake decreases circulating catecholamines and prevents diet-induced insulin resistance and hypertension in rats
      Available formats
      ×

Copyright

Corresponding author

*Corresponding author: Dr Maria Pedro Guarino, fax +351 218803078, email maria.guarino@fcm.unl.pt

References

Hide All
1 Kashyap, SR & Defronzo, RA (2007) The insulin resistance syndrome: physiological considerations. Diab Vasc Dis Res 4, 1319.
2 Riksen, NP, Rongen, GA & Smits, P (2009) Acute and long-term cardiovascular effects of coffee: implications for coronary heart disease. Pharmacol Ther 121, 185191.
3 Moisey, LL, Kacker, S, Bickerton, AC, et al. (2008) Caffeinated coffee consumption impairs blood glucose homeostasis in response to high and low glycemic index meals in healthy men. Am J Clin Nutr 87, 12541261.
4 Keijzers, GB, De Galan, BE, Tack, CJ, et al. (2002) Caffeine can decrease insulin sensitivity in humans. Diabetes Care 25, 364369.
5 van Dam, RM, Willett, WC, Manson, JE, et al. (2006) Coffee, caffeine, and risk of type 2 diabetes: a prospective cohort study in younger and middle-aged U.S. women. Diabetes Care 29, 398403.
6 van Dam, RM & Feskens, EJ (2002) Coffee consumption and risk of type 2 diabetes mellitus. Lancet 360, 14771478.
7 Noordzij, M, Uiterwaal, CS, Arends, LR, et al. (2005) Blood pressure response to chronic intake of coffee and caffeine: a meta-analysis of randomized controlled trials. J Hypertens 23, 921928.
8 Jee, SH, He, J, Whelton, PK, et al. (1999) The effect of chronic coffee drinking on blood pressure: a meta-analysis of controlled clinical trials. Hypertension 33, 647652.
9 Geleijnse, JM (2008) Habitual coffee consumption and blood pressure: an epidemiological perspective. Vasc Health Risk Manag 4, 963970.
10 Conde, SV, Pinto, P, Bárbara, C, et al. (2009) Should caffeine be avoided in obstructive sleep apnea? In European Respiratory Society Annual Congress, Vienna, Austria: 2009. Abstracts book P3729.
11 Arnlov, J, Vessby, B & Riserus, U (2004) Coffee consumption and insulin sensitivity. JAMA 291, 11991201.
12 Greenberg, JA, Boozer, CN & Geliebter, A (2006) Coffee, diabetes, and weight control. Am J Clin Nutr 84, 682693.
13 Zheng, G, Sayama, K, Okubo, T, et al. (2004) Anti-obesity effects of three major components of green tea, catechins, caffeine and theanine, in mice. In Vivo 18, 5562.
14 Lopez-Garcia, E, van Dam, RM, Rajpathak, S, et al. (2006) Changes in caffeine intake and long-term weight change in men and women. Am J Clin Nutr 83, 674680.
15 Greenberg, JA, Axen, KV, Schnoll, R, et al. (2005) Coffee, tea and diabetes: the role of weight loss and caffeine. Int J Obes (Lond) 29, 11211129.
16 Wu, T, Willett, WC, Hankinson, SE, et al. (2005) Caffeinated coffee, decaffeinated coffee, and caffeine in relation to plasma C-peptide levels, a marker of insulin secretion, in U.S. women. Diabetes Care 28, 13901396.
17 Cheung, WT, Lee, CM & Ng, TB (1988) Potentiation of the anti-lipolytic effect of 2-chloroadenosine after chronic caffeine treatment. Pharmacology 36, 331339.
18 Astrup, A, Breum, L, Toubro, S, et al. (1992) The effect and safety of an ephedrine/caffeine compound compared to ephedrine, caffeine and placebo in obese subjects on an energy restricted diet. A double blind trial. Int J Obes Relat Metab Disord 16, 269277.
19 Sung, BH, Whitsett, TL, Lovallo, WR, et al. (1994) Prolonged increase in blood pressure by a single oral dose of caffeine in mildly hypertensive men. Am J Hypertens 7, 755758.
20 Sung, BH, Lovallo, WR, Whitsett, T, et al. (1995) Caffeine elevates blood pressure response to exercise in mild hypertensive men. Am J Hypertens 8, 11841188.
21 Pincomb, GA, Lovallo, WR, McKey, BS, et al. (1996) Acute blood pressure elevations with caffeine in men with borderline systemic hypertension. Am J Cardiol 77, 270274.
22 Benowitz, NL (1990) Clinical pharmacology of caffeine. Annu Rev Med 41, 277288.
23 Fredholm, BB, Battig, K, Holmen, J, et al. (1999) Actions of caffeine in the brain with special reference to factors that contribute to its widespread use. Pharmacol Rev 51, 83133.
24 Robertson, D, Wade, D, Workman, R, et al. (1981) Tolerance to the humoral and hemodynamic effects of caffeine in man. J Clin Invest 67, 11111117.
25 Myers, MG (1988) Effects of caffeine on blood pressure. Arch Intern Med 148, 11891193.
26 Holtzman, SG & Finn, IB (1988) Tolerance to behavioral effects of caffeine in rats. Pharmacol Biochem Behav 29, 411418.
27 Gasior, M, Shoaib, M, Yasar, S, et al. (1999) Acquisition of nicotine discrimination and discriminative stimulus effects of nicotine in rats chronically exposed to caffeine. J Pharmacol Exp Ther 288, 10531073.
28 Shearer, J, Severson, DL, Su, L, et al. (2009) Partial A1 adenosine receptor agonist regulates cardiac substrate utilization in insulin-resistant rats in vivo. J Pharmacol Exp Ther 328, 306311.
29 Ribeiro, RT, Lautt, WW, Legare, DJ, et al. (2005) Insulin resistance induced by sucrose feeding in rats is due to an impairment of the hepatic parasympathetic nerves. Diabetologia 48, 976983.
30 Gasior, M, Jaszyna, M, Munzar, P, et al. (2002) Caffeine potentiates the discriminative-stimulus effects of nicotine in rats. Psychopharmacology (Berl) 162, 385395.
31 Rodriguez-Perez, JC, Losada, A, Anabitarte, A, et al. (1997) Effects of the novel multiple-action agent carvedilol on severe nephrosclerosis in renal ablated rats. J Pharmacol Exp Ther 283, 336344.
32 Monzillo, LU & Hamdy, O (2003) Evaluation of insulin sensitivity in clinical practice and in research settings. Nutr Rev 61, 397412.
33 Kim, SP, Ellmerer, M, Van Citters, GW, et al. (2003) Primacy of hepatic insulin resistance in the development of the metabolic syndrome induced by an isocaloric moderate-fat diet in the dog. Diabetes 52, 24532460.
34 Ismail, NA, El Denshary, EE & Montague, W (1977) Adenosine and the regulation of insulin secretion by isolated rat islets of Langerhans. Biochem J 164, 409413.
35 Kanazawa, M, Xue, CY, Kageyama, H, et al. (2003) Effects of a high-sucrose diet on body weight, plasma triglycerides, and stress tolerance. Nutr Rev 61, S27S33.
36 Delarue, J & Magnan, C (2007) Free fatty acids and insulin resistance. Curr Opin Clin Nutr Metab Care 10, 142148.
37 Lewis, GF, Carpentier, A, Adeli, K, et al. (2002) Disordered fat storage and mobilization in the pathogenesis of insulin resistance and type 2 diabetes. Endocr Rev 23, 201229.
38 Egawa, T, Hamada, T, Kameda, N, et al. (2009) Caffeine acutely activates 5′adenosine monophosphate-activated protein kinase and increases insulin-independent glucose transport in rat skeletal muscles. Metabolism 58, 16091617.
39 Raney, MA & Turcotte, LP (2008) Evidence for the involvement of CaMKII and AMPK in Ca2+-dependent signaling pathways regulating FA uptake and oxidation in contracting rodent muscle. J Appl Physiol 104, 13661373.
40 Dhalla, AK, Chisholm, JW, Reaven, GM, et al. (2009) A1 adenosine receptor: role in diabetes and obesity. Handb Exp Pharmacol 193, 271295.
41 Espinal, J, Challiss, RA & Newsholme, EA (1983) Effect of adenosine deaminase and an adenosine analogue on insulin sensitivity in soleus muscle of the rat. FEBS Lett 158, 103106.
42 Han, DH, Hansen, PA, Nolte, LA, et al. (1998) Removal of adenosine decreases the responsiveness of muscle glucose transport to insulin and contractions. Diabetes 47, 16711675.
43 Vergauwen, L, Hespel, P & Richter, EA (1994) Adenosine receptors mediate synergistic stimulation of glucose uptake and transport by insulin and by contractions in rat skeletal muscle. J Clin Invest 93, 974981.
44 Rusing, D, Muller, CE & Verspohl, EJ (2006) The impact of adenosine and A(2B) receptors on glucose homoeostasis. J Pharm Pharmacol 58, 16391645.
45 Figler, RA, Wang, G, Srinivasan, S, et al. (2011) Links between insulin resistance, adenosine A2B receptors, and inflammatory markers in mice and humans. Diabetes 60, 669679.
46 Umemura, T, Ueda, K, Nishioka, K, et al. (2006) Effects of acute administration of caffeine on vascular function. Am J Cardiol 98, 15381541.
47 Scharf, G, Prustomersky, S & Huber, WW (2001) Elevation of glutathione levels by coffee components and its potential mechanisms. Adv Exp Med Biol 500, 535539.
48 Varma, SD & Hegde, KR (2010) Prevention of oxidative damage to lens by caffeine. J Ocul Pharmacol Ther 26, 7377.
49 Mytilineou, C, Kramer, BC & Yabut, JA (2002) Glutathione depletion and oxidative stress. Parkinsonism Relat Disord 8, 385387.
50 Jackisch, R, Fehr, R & Hertting, G (1985) Adenosine: an endogenous modulator of hippocampal noradrenaline release. Neuropharmacology 24, 499507.
51 Tseng, CJ, Chan, JY, Lo, WC, et al. (2001) Modulation of catecholamine release by endogenous adenosine in the rat adrenal medulla. J Biomed Sci 8, 389394.
52 Robertson, D, Frolich, JC, Carr, RK, et al. (1978) Effects of caffeine on plasma renin activity, catecholamines and blood pressure. N Engl J Med 298, 181186.
53 Debrah, K, Haigh, R, Sherwin, R, et al. (1995) Effect of acute and chronic caffeine use on the cerebrovascular, cardiovascular and hormonal responses to orthostasis in healthy volunteers. Clin Sci (Lond) 89, 475480.
54 Carella, AM, Antonucci, G, Conte, M, et al. (2010) Antihypertensive treatment with β-blockers in the metabolic syndrome: a review. Curr Diabetes Rev 6, 215221.
55 Bhatt, P, Makwana, D, Santani, D, et al. (2007) Comparative effectiveness of carvedilol and propranolol on glycemic control and insulin resistance associated with l-thyroxin-induced hyperthyroidism – an experimental study. Can J Physiol Pharmacol 85, 514520.
56 Kveiborg, B, Christiansen, B, Major-Petersen, A, et al. (2006) Metabolic effects of β-adrenoceptor antagonists with special emphasis on carvedilol. Am J Cardiovasc Drugs 6, 209217.
57 Corsetti, G, Pasini, E, Assanelli, D, et al. (2007) Acute caffeine administration decreased NOS and Bcl2 expression in rat skeletal muscles. Pharmacol Res 55, 96103.
58 Ofluoglu, E, Pasaoglu, H & Pasaoglu, A (2009) The effects of caffeine on l-arginine metabolism in the brain of rats. Neurochem Res 34, 395399.
59 Guarino, MP & Macedo, MP (2006) Co-administration of glutathione and nitric oxide enhances insulin sensitivity in Wistar rats. Br J Pharmacol 147, 959965.
60 Guarino, MP, Correia, NC, Raposo, J, et al. (2001) Nitric oxide synthase inhibition decreases output of hepatic insulin sensitizing substance (HISS), which is reversed by SIN-1 but not by nitroprusside. Proc West Pharmacol Soc 44, 2526.
61 Shi, D & Daly, JW (1999) Chronic effects of xanthines on levels of central receptors in mice. Cell Mol Neurobiol 19, 719732.
62 Stonehouse, AH, Adachi, M, Walcott, EC, et al. (2003) Caffeine regulates neuronal expression of the dopamine 2 receptor gene. Mol Pharmacol 64, 14631473.

Keywords

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed