Hostname: page-component-848d4c4894-jbqgn Total loading time: 0 Render date: 2024-06-28T19:49:02.895Z Has data issue: false hasContentIssue false
  • English
  • Français

Beef conjugated linoleic acid isomers reduce human cancer cell growth even when associated with other beef fatty acids

Published online by Cambridge University Press:  08 March 2007

Anne De La Torre ,
Eric Debiton ,
Pierre Juanéda ,
Denys Durand ,
Jean-Michel Chardigny ,
Chantal Barthomeuf ,
Dominique Bauchart  and
Dominique Gruffat
Anne De La Torre
Affiliation:
INRA, Unité de Recherche sur les Herbivores, Equipe Nutriments et Métabolismes, 63122 Saint Genés-Champanelle, France
Eric Debiton
Affiliation:
Laboratoire de Pharmacognosie/Biotechnologies, UMR 484 INSERM, 63005 Clermont-Ferrand, France
Pierre Juanéda
Affiliation:
INRA, Unité de Nutrition Lipidique, 21065 Dijon, France
Denys Durand
Affiliation:
INRA, Unité de Recherche sur les Herbivores, Equipe Nutriments et Métabolismes, 63122 Saint Genés-Champanelle, France
Jean-Michel Chardigny
Affiliation:
INRA, Unité de Nutrition Lipidique, 21065 Dijon, France
Chantal Barthomeuf
Affiliation:
Laboratoire de Pharmacognosie/Biotechnologies, UMR 484 INSERM, 63005 Clermont-Ferrand, France
Dominique Bauchart
Affiliation:
INRA, Unité de Recherche sur les Herbivores, Equipe Nutriments et Métabolismes, 63122 Saint Genés-Champanelle, France
Dominique Gruffat*
Affiliation:
INRA, Unité de Recherche sur les Herbivores, Equipe Nutriments et Métabolismes, 63122 Saint Genés-Champanelle, France
*
*corresponding author: fax +33 04 73 62 46 39, email gruffat@sancy.clermont.inra.fr
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Although many data are available concerning anticarcinogenic effects of industrial conjugated linoleic acid (CLA), few studies have reported the antitumour properties of CLA mixtures originating from ruminant products. The aim of the present study was to investigate the in vitro antiproliferative effects of beef CLA mixtures on breast, lung, colon, melanoma and ovarian human cancer cell lines. For this purpose, four fatty acid (FA) extracts prepared from beef lipid and varying in their CLA composition, their corresponding purified CLA-enriched fractions, and mixtures of pure synthetic CLA, the composition of which reproduced that of the four selected beef samples, were tested on cancer cell lines. Cancer cells were exposed for 48h to medium containing 100μm-FA and their proliferation was determined by quantifying cellular DNA content (Hoechst 33342 dye). Compared with cells incubated without FA, the number of cancer cells was reduced from 25 to 67% (P<0·0001) following FA treatment. Antiproliferative effects of CLA mixtures varied in magnitude according to the source of FA, the CLA composition and the cell lines. CLA mixtures naturally present in beef inhibited the proliferation of human cancer cell lines, a high content in cis-trans isomers allowing the most important antiproliferative effect. Beef total FA exhibited a greater growth-inhibitory activity than their corresponding CLA-enriched fractions. These results suggested that either beef FA other than beef CLA could possess antiproliferative properties and/or the existence of complementary effects of non-conjugated FA and CLA, which could favour the antiproliferative properties of beef total FA.

Keywords

BeefFatty acidsConjugated linoleic acidHuman cancer cellsGrowth inhibition
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 2 , February 2006 , pp. 346 - 352
Copyright
Copyright © The Nutrition Society 2006

References

Awad, ABBegdache, LAFink, CSEffect of sterols and fatty acids on growth and triglyceride accumulation in 3T3-L1 cells. J Nutr Biochem 2000 11 153–158CrossRefGoogle Scholar
Awad, ABHerrmann, TFink, CSHorvath, PJ18:1 n7 fatty acids inhibit growth and decrease inositol phosphate release in HT-29 cells compared to n-9 fatty acids Cancer Lett 1995 91 55–61CrossRefGoogle Scholar
Bauchart, DAurousseau, BPost-prandial lipids in blood plasma of prerumiant calves. J Dairy Sci 1981 64 2033–2042CrossRefGoogle Scholar
Begin, MEElls, GHorrobin, DFPolyunsaturated fatty acidinduced cytotoxicity against tumor cells and its relationship to lipid peroxidation. J Natl Cancer Inst 1988 80 188–194CrossRefGoogle ScholarPubMed
Belury, MADietary conjugated linoleic acid in health: physiological effects and mechanisms of action. Annu Rev Nutr 2002 22 505–531Google Scholar
Belury, MAVanden Hauvel, JPProtection against cancer and heart disease by CLA: potential mechanisms of action. Nutr Dis Update 1997 1 58–63Google Scholar
Booyens, JEngelbrecht, PLe Roux, SLouwrens, CCvan der Merwe, CFKatzeff, IESome effects of the essential fatty acids linoleic acid and alpha-linolenic acid and of their metabolites gamma-linolenic acid, arachidonic acid, eicosapentaenoic acid, docosahexaenoic acid, and of prostaglandins A1 and E1 on the proliferation of human osteogenic sarcoma cells in culture. Prost Leuk Med 1984 15 15–33Google Scholar
Christie, WWA practical guide to the analysis of conjugated linoleic acid. Inform 2001 12 147–152Google Scholar
Corl, BABarbano, BMBauman, DEIP, CCis-9, trans-11 CLA derived endogenously from trans-11 18:1 reduces cancer risk in rats. J Nutr 2003 133 2893–2900Google Scholar
Cruz-Hernandez, CDeng, ZZhou, JHill, ARYurawecz, MPDelmonte, PMossoba, MMDugan, MEKramer, JKMethods for analysis of conjugated linoleic acids and trans-18:1 isomers in dairy fats by using a combination of gas chromatography, silverion thin-layer chromatography/gas chromatography, and silverion liquid chromatography. J AOAC Int 2004 87 545–562Google Scholar
Das, unEssential fatty acids, lipid peroxidation and apoptosis. Prost Leuk Ess Fatty Acids 1999 61 157–163CrossRefGoogle ScholarPubMed
Debiton, EMadelmont, JCLegault, JBarthomeuf, CSanguinarine-induced apoptosis is associated with an early and severe cellular glutathione depletion. Cancer Chem Pharmacol 2003 51 474–482Google Scholar
Falconer, JSRoss, JAFearon, KCHawkins, RAO'Riordain, MGCarter, DCEffect of eicosapentaenoic acid and other fatty acids on the growth in vitro of human pancreatic cancer cell lines. Br J Cancer 1994 69 826–832Google Scholar
Folch, JLees, MSloane-Stanley, GHSA simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 1957 226 497–505CrossRefGoogle ScholarPubMed
Gnädig, SRickert, RSebedio, JLSteinhart, HConjugated linoleic acid (CLA): physiological effects and production. Eur J Lipid Sci Technol 2001 103 56–613.0.CO;2-1>CrossRefGoogle Scholar
Griinari, JMBauman, DEBiosynthesis of conjugated linoleic acid and its incorporation into meat and milk in ruminants Sebedio, J-LChristie, WWAdlof, RChampaign, IL:In Advances in Conjugated Linoleic Acid Research pp. vol.AOCS Press. 1999 1 180–200Google Scholar
IP, CBanni, SAngioni, ECarta, GMcginley, JThompson, HJBarbano, DBauman, DConjugated linoleic acid-enriched butter fat alters mammary gland morphogenesis and reduces cancer risk in rats. J Nutr 1999 129 2135–2142Google Scholar
Jacubovich, RCabrillat, HGerlier, DBailly, MDore, JFTumourigenic phenotypes of human melanoma cell lines in nude mice determined by an active antitumour mechanism. Br J Cancer 1985 51 335–345Google Scholar
Kelly, GSConjugated linoleic acid: a review. Alt Med Rev 2001 6 367–382Google ScholarPubMed
Kim, EJHolthuizen, PEPark, HSHa, YLJung, KCPark, JHTrans-10, cis-12-conjugated linoleic acid inhibits Caco-2 colon cancer cell growth. Am J Physiol 2002 283 G357–G367Google Scholar
Kritchevsky, DAntimutagenic and some other effects of conjugated linoleic acid. Br J Nutr 2000 83 459–465CrossRefGoogle ScholarPubMed
Kumar, GSDas, UNFree-radical-dependent suppression of growth of mouse myeloma cells by alpha-linolenic and eicosanoic acid in vitro. Cancer Lett 1995 92 27–38Google Scholar
McMillan, DHeys, SAlastair, WNoble, BPurasiri, PDeehan, DEremin, ODifferential responses of human tumour cells to polyunsaturated fatty acids: stimulation of proliferation of a colon tumour cell line by docosahexaenoic acid. Intern J Onc 1995 7 927–933Google Scholar
Miller, AMcGrath, EStanton, CDevery, RVaccenic acid (t11-18:1) is converted to c9, t11-CLA in MCF-7 and SW480 cancer cells. Lipids 2003 38 623632Google Scholar
Miller, AStanton, CDevery, RCis-9, trans-11- and trans-10, cis-12-conjugated linoleic acid isomers induce apoptosis in cultured SW480 cells. Anticancer Res 2002 22 3879–3887Google ScholarPubMed
Morris, LJSeparation of lipids by silver ion chromatography. J Lipid Res 1966 7 717–732Google Scholar
Nano, JLNobili, CGirard-Pipau, FRampal, PEffects of fatty acids on the growth of Caco-2 cells Prost Leukot Essent Fatty Acids 2003 69 207–215Google Scholar
Ochoa, JJFarquharson, AJGrant, IMoffat, LEHeys, SDWahle, KWConjugated linoleic acids (CLAs) decrease prostate cancer cell proliferation: different molecular mechanisms for cis-9, trans-11 and trans-10, cis-12 isomers. Carcinogenesis 2004 25 1185–1191CrossRefGoogle ScholarPubMed
O'Shea, MDevery, RLawless, FMurphy, JStanton, CMilk fat conjugated linoleic acid (CLA) inhibits growth of human mammary MCF-7 cancer cells. Anticancer Res 2000 20 3591–3601Google Scholar
Palombo, JDGanguly, ABistrian, BRMenard, MPThe antiproliferative effects of biologically active isomers of conjugated linoleic acid on human colorectal and prostatic cancer cells. Cancer Lett 2002 177 163–172Google Scholar
Pariza, MWPark, YCook, MEMechanisms of action of conjugated linoleic acid: evidence and speculation. Proc Soc Exp Bio Med 2000 223 8–13Google ScholarPubMed
Pariza, MWPark, YCook, METhe biologically active isomers of conjugated linoleic acid.Prog Lipid Res 2001 40 283–298CrossRefGoogle ScholarPubMed
SAS Institute Inc. SAS/STAT User's Guide Cary, NC version 6, 4th ed. NCSAS Institute Inc. 1989Google Scholar
Sébédio, JLPrevost, JGrandgirard, AHeat treatment of vegetable oils. Isolation of cyclic fatty acid monomers from heated sunflower and linseed oils. J Am Oil Chem Soc 1987 64 1026–1032Google Scholar
Shultz, TDChew, BSeaman, WRLuedecke, LOInhibitory effect of conjugated dienoic derivatives of linoleic acid and betacarotene on the in vitro growth of human cancer cells. Cancer Lett 1992 63 125–133CrossRefGoogle ScholarPubMed