Skip to main content Accessibility help
×
Home

Assessment of the potential of a boron–fructose additive in counteracting the toxic effect of Fusarium mycotoxins

  • Ionelia Taranu (a1), Daniela E. Marin (a1), Gina Manda (a2), Monica Motiu (a1), Ionela Neagoe (a2), Cristina Tabuc (a1), Mariana Stancu (a1) and Margareta Olteanu (a1)...

Abstract

Trichotecenes are mycotoxins produced by Fusarium sp., which may contaminate animal feeds and human food. A feeding trial was conducted to evaluate the effect of a fusarotoxin-contaminated diet, and to explore the counteracting potential of a calcium fructoborate (CFrB) additive on performance, typical health biochemistry parameters and immune response in weaned pigs. A naturally contaminated maize, containing low doses of deoxynivalenol, zearalenone, fumonisins and T-2/HT-2 toxins (1790, 20, 0·6 and 90 parts per billion), was included in a maize–soyabean meal diet, and given ad libitum to eight weaned piglets (two groups: four pigs/group) for a period of 24 d. CFrB was administered to one of the contaminated groups and to another four piglets as a daily supplement, following the manufacturer's recommendation. A decrease in performance was observed in contaminated animals at this concentration of feed toxins, which was ameliorated by the dietary CFrB supplementation. Fusarium toxins also altered the pig immune response by increasing (P < 0·05) the ex vivo peripheral blood mononuclear cell proliferation (111·7 % in comparison with control), the respiratory burst of porcine granulocytes (15·4 % for responsive cells v. 5·1 % for unstimulated cells and 70·95 v. 22·65 % for stimulated cells, respectively), the percentage of peripheral T, CD3+, CD3+CD4+ and CD3+CD8+ subsets and the synthesis of IL-1β, TNF-α and IL-8 (123·8, 217·1 and 255·1 %, respectively). The diet containing the CFrB additive reduced these exacerbated cellular immune responses induced by Fusarium toxins. However, consumption of CFrB did not counteract the effect of mycotoxins on biochemistry parameters, and increased plasma IgM and IgG of contaminated pigs.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Assessment of the potential of a boron–fructose additive in counteracting the toxic effect of Fusarium mycotoxins
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Assessment of the potential of a boron–fructose additive in counteracting the toxic effect of Fusarium mycotoxins
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Assessment of the potential of a boron–fructose additive in counteracting the toxic effect of Fusarium mycotoxins
      Available formats
      ×

Copyright

Corresponding author

*Corresponding author: I. Taranu, fax +40 213512080, email ionelia.taranu@ibna.ro

References

Hide All
1 Smith, TK, McMillan, EG & Castillo, JB (1997) Effect of feeding blends of Fusarium mycotoxin-contaminated grains containing deoxynivalenol and fusaric acid on growth and feed consumption of immature swine. J Anim Sci 75, 21842191.
2 Agence Francaise de Securite Alimentaire – AFSA (2009) Report: Evaluation des risques liés à la présence de mycotoxines dans la chaîne alimentaire (Report: Assessment of Risk Associated with the Presence of Mycotoxins in the Food Chain). http://www.afssa.fr/index.htm.
3 Pestka, JJ & Smolinski, AT (2005) Deoxynivalenol: toxicology and potential effects on humans. J Toxicol Environ Health B Crit Rev 8, 3969.
4 Lillehoj, EB (1983) Effect of environmental and cultural factors on aflatoxin contamination of developing corn kernels. In Aflatoxins and Aspergillus flavus in Corn, pp. 27–34 [Diener, UL, Asquith, RL and Dickens, JW, editors]. Aubum University. Southern Cooperative Series Bulletin 279. Auburn, AL: Alabama Agricultural Experiment Station.
5 Bhatnagar, D, Lillehoj, EB, Bennett, JW, et al. (1991) Biological detoxification of mycotoxins. In Mycotoxin and Animal Food, Chapter 36, pp. 815826. Boca Raton, FL: CRC Press.
6 Kabak, B, Dobson, ADW & Işl, V (2006) Strategies to prevent mycotoxin contamination of food and animal feed: a review. Crit Rev Food Sci Nutr 46, 593619.
7 Bata, A & Lasztity, R (1999) Detoxification of mycotoxin-contaminated food and feed by microorganisms. Trends Food Sci Technol 10, 223228.
8 Karlovsky, P (1999) Biological detoxification of fungal toxins and its use in plant breeding, feet, and food production. Nat Toxins 7, 123.
9 Avantaggiato, G, Havenaar, R & Visconti, A (2007) Assessment of the multi-mycotoxin-binding efficacy of a carbon/aluminosilicate-based product in an in vitro gastrointestinal model. J Agric Food Chem 55, 48104819.
10 Bata, A & Lasztity, R (1999) Detoxification of mycotoxin-contaminated food and feed by microorganisms. Trends Food Sci Technol 10, 223228.
11 Döll, S, Gericke, S, Dänicke, S, et al. (2005) The efficacy of a modified aluminosilicate as a detoxifying agent in Fusarium toxin contaminated maize containing diets for piglets. J Anim Physiol Anim Nutr 89, 342358.
12 The European Food Safety Authority (EFSA) Journal (2009) Review of Mycotoxin-detoxifying Agents used as Feed Additives: Mode of Action, Efficacy and Feed/food Safety. Scientific Report Q-2009-00839. http://www.efsa.europa.eu/en/scdocs/scdoc/22e.htm.
13 The European Food Safety Authority (EFSA) Journal (2005) Opinion of the Scientific Panel on Additives and Products or Substances Used in Animal Feed on a Request from the Commission on the Safety of the Product “Biomin BBSH 797” for Piglets, Pigs for Fattening and Chickens for Fattening. http://www.efsa.europa.eu.
14 Galvano, F, Piva, A, Ritieni, A, et al. (2001) Dietary strategies to counteract the effects of mycotoxins: a review. J Food Prot 64, 120131.
15 Pié, S, Lallès, JP, Blazy, F, et al. (2004) Weaning is associated with an upregulation of expression of inflammatory cytokines in the intestine of piglets. J Nutr 134, 641647.
16 EC/576 (2006) European Commission recommendation on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 and fumonisins in products intended for animal feeding. Official J European Union I, 229/7229/9.
17 Rotaru, P, Scorei, R, Harabor, A, et al. (2010) Thermal analysis of a calcium fructoborate sample. Thermochim Acta 506, 813.
18 The Romanian Standardized Association (ASRO) Standardized Bulletin (2010) SR ISO 6496/2001. http://www.asro.ro.
19 The Romanian Standardized Association (ASRO) Standardized Bulletin (2010) SR EN ISO 5983-2/2009. http://www.asro.ro.
20 The Romanian Standardized Association (ASRO) Standardized Bulletin (2010) SR ISO 6492/2001. http://www.asro.ro.
21 The Romanian Standardized Association (ASRO) Standardized Bulletin (2010) SR EN ISO 6865/2002. http://www.asro.ro.
22 The Romanian Standardized Association (ASRO) Standardized Bulletin (2010) SR EN ISO 2171/2010. http://www.asro.ro.
23 Marin, DE, Taranu, I, Pascale, F, et al. (2006) Sex-related differences in the immune response of weanling piglets exposed to low doses of fumonisin extract. Br J Nutr 95, 19.
24 Etienne, M (2007) Effets biologiques et physiologiques d'une mycotoxine, le déoxynivalénol (DON), chez le porc (Biological and physiological effects of deoxynivalenol (DON), a mycotoxin, in the pig). Journées Rech Porcine 39, 407418.
25 Grosjean, F, Callu, P, Pinton, P, et al. (2003) Quantification des effets de la consommation de déoxynivalénol (DON) par le porcelet sevré (Quantification of the effects of deoxynivalenol consumption by weaned piglets). Journées Rech Porcine 35, 443450.
26 Swamy, HVLN, Smith, TK, MacDonald, EJ, et al. (2002) Effects of feeding a blend of grains naturally contaminated with mycotoxins Fusarium on swine performance, brain regional neurochemistry, and serum chemistry and the efficacy of a polymeric glucomannan mycotoxin adsorbent. J Anim Sci 80, 32573267.
27 Foster, BC, Trenholm, HL, Friend, DW, et al. (1986) Evaluation of different sources of deoxynivalenol (vomitoxin) fed to swine. Can J Anim Sci 66, 11491154.
28 Friend, DW, Trenholm, HL, Elliot, JI, et al. (1982) Effect of feeding vomitoxin-contaminated wheat to pigs. Can J Anim Sci 62, 12111222.
29 Armstrong, TA, Flowers, WL, Spears, JW, et al. (2002) Long-term effects of boron supplementation on reproductive characterstics and bone mechanical properties in gilts. J Anim Sci 80, 154161.
30 Armstrong, TA & Spears, JW (2003) Effect of boron supplementation of pig diets on the production of tumor necrosis factor-alpha and interferon-gamma. J Anim Sci 81, 25522561.
31 Gutzwiller, A, Czegledi, L, Stoll, P, et al. (2007) Effects of Fusarium toxins on growth, humoral immune response and internal organs in weaner pigs, and the efficacy of apple pomace as an antidote. J Anim Physiol Anim Nutr 91, 432438.
32 Wetscherek, W, Huber, H & Lew, J (1998) Einsatz von mit Mycotoxinen kontaminiertern Mais und von Detoxifikationsmitteln in der Schweinemast (Use of corn contaminated with mycotoxins and detoxifying in pigs). Proc Soc Nutr Physiol 7, 93.
33 Swamy, HVLN, Smith, TK, Cotter, PF, et al. (2002) Effects of feeding blends of grains naturally contaminated with Fusarium mycotoxins on production and metabolism in broilers. Poult Sci 81, 966975.
34 Oswald, IP, Marin, DE, Bouhet, S, et al. (2005) Immunotoxicological risk of mycotoxins for domestic animals. Food Add Contam 22, 354360.
35 Pestka, JJ, Zhou, HR, Moon, Y, et al. (2004) Cellular and molecular mechanisms for immune modulation by deoxynivalenol and other trichothecenes: unravelling a paradox. Toxicol Lett 153, 6173.
36 Meky, FA, Hardie, LJ, Evans, SW, et al. (2001) Deoxynivalenol-induced immunomodulation of human lymphocyte proliferation and cytokine production. Food Chem Toxicol 39, 827836.
37 Grosjean, F, Pinton, P, Callu, P, et al. (2007) Effets de la consommation par le porcelet sevré d'aliment contenant du blé naturellement fusarié (Effects of consumption of diet including Fusarium naturally contaminated wheat in weaned piglets). Journees Rech. Porcine 39, 427428.
38 Goyarts, T, Danicke, S, Tiemann, U, et al. (2006) Effect of the Fusarium toxin deoxynivalenol (DON) on IgA, IgM and IgG concentrations and proliferation of porcine blood lymphocytes. Toxicol In vitro 20, 858867.
39 Pinton, P, Accensi, F & Beauchamp, E (2006) Effets de la consommation d'aliment naturellement contaminé par du déoxynivalénol (DON) sur la réponse vaccinale du porc (Effects of consumption of naturally contaminated feed by deoxynivalenol (DON) on the vaccinal immune response in pigs). Journées Rech Porcine 38, 399406.
40 Rotter, BA, Prelusky, DB & Pestka, JJ (1996) Toxicology of deoxynivalenol (vomitoxin). J Toxicol Environ Health 48, 134.
41 Accensi, F, Pinton, P, Callu, P, et al. (2006) Ingestion of low doses of deoxynivalenol does not affect haematological, biochemical, or immune responses of piglets. J Anim Sci 84, 19351942.
42 Danicke, S, Valenta, H, Klobasa, F, et al. (2004) Effects of graded levels of Fusarium toxin contaminated wheat in diets for fattening pigs on growth performance, nutrient digestibility, deoxynivalenol balance and clinical serum characteristics. Arch Anim Nutr 58, 117.
43 Doll, S, Danicke, S, Ueberschar, KH, et al. (2003) Effects of graded levels of Fusarium toxin contaminated maize in diets for female weaned piglets. Arch Anim Nutr 57, 311334.
44 Swamy, HVLN, Smith, TK, MacDonald, EJ, et al. (2003) Effects of feeding a blend of grains naturally contamined with Fusarium mycotoxins on growth and immunological measurements of starter pigs, and the efficacy of a polymeric glucomannan mycotoxin adsorbent. J Anim Sci 81, 27922803.
45 Girish, CK, Smith, TK, Boermans, HJ, et al. (2010) Effects of dietary Fusarium mycotoxins on intestinal lymphocyte subset populations, cell proliferation and histological changes in avian lymphoid organs. Food Chem Toxicol 48, 30003007.
46 Pinton, P, Royer, E, Accensi, F, et al. (2004) Effets zootechniques et immunitaires de la consommation d'aliment naturellement contaminé par du déoxynivalénol (DON) chez le porc en phase de croissance ou de finition (Effects of feeding growing or finishing pigs with deoxynivalenol (DON) natually contaminated feed on growth performance and immunological status). Journées Rech Porcine 36, 301308.
47 Grosjean, F, Taranu, I, Skiba, F, et al. (2002) Comparaisons de blés fusariés naturellement à des blés sains, dans l'alimentation du porcelet sevré (Comparisons of different naturally Fusarium-contaminated wheats with uncontaminated wheats in weaned piglet diets). Journées Rech Porcine 34, 333339.
48 Scorei, RI, Ciofrangeanu, C, Ion, R, et al. (2010) In vitro effects of calcium fructoborate upon production of inflammatory mediators by LPS-stimulated RAW 264.7 macrophages. Biol Trace Elem Res 135, 334344.
49 Scorei, R, Cimpoiasu, VM & Iordachescu, D (2005) In vitro evaluation of the antioxidant activity of calcium fructoborate. Biol Trace Elem Res 107, 127134.
50 Luo, D & Eckhert, CD (2000) Boron responsive human genes. FASEB J 14, A478.
51 Hunt, CD & Idso, JP (1999) Dietary boron as physiological regulator of the normal inflammatory response: a review and current reaserch progress. J Trace Elem Exo Med 12, 221223.
52 Nielsen, FH (1997) Boron in human and animal nutrition. Plant Soil 193, 199208.
53 Nielsen, FH (1994) Biochemical and physiologic consequences of boron deprivation in humans. Environ Health Perspect 102, Suppl. 7, 5963.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed