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An investigation of the effects of intravenous administration of thiomolybdate on copper metabolism in chronic Cu-poisoned sheep

Published online by Cambridge University Press:  09 March 2007

S. R. Gooneratne ,
J. McC. Howell  and
J. M. Gawthorne
S. R. Gooneratne
Affiliation:
Division of Veterinary Biology, School of Veterinary Studies, Murdoch University, Murdoch, Western Australia 6150, Australia
J. McC. Howell
Affiliation:
Division of Veterinary Biology, School of Veterinary Studies, Murdoch University, Murdoch, Western Australia 6150, Australia
J. M. Gawthorne
Affiliation:
Division of Veterinary Biology, School of Veterinary Studies, Murdoch University, Murdoch, Western Australia 6150, Australia
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Abstract

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1. Fourteen sheep were dosed repeatedly with a solution of copper sulphate (2 g/l) in order to induce chronic copper poisoning and four similar undosed animals acted as controls.

2. Thiomolybdate (TM) was intravenously administered to all control sheep and to all except two of the test sheep. A variety of biochemical factors were studied before and after injections of TM.

3. The direct-reacting Cu, whole-blood Cu and plasma Cu concentrations were elevated in animals given TM injections and at the ‘haemolytic crisis’ of untreated chronic Cu-poisoned animals. But most of the increased Cu observed on injecting TM was insoluble in trichloroacetic acid (TCA) and did not enter erythrocytes. The results indicate that uptake of Cu by erythrocytes is essential for haemolysis to occur and that for this to happen the Cu must be in a direct reacting, TCA-soluble form.

4. Increased amounts of Cu were excreted in the urine at haemolysis and at the commencement of TM injections. High levels of direct-reacting Cu were found in plasma at these times.

5. Marked changes were not found in caeruloplasmin activity, packed cell volume or the osmotic fragility of erythrocytes except at haemolysis. TM injections did not alter these factors in any of the sheep studied.

Type
General Nutrition
Information
British Journal of Nutrition , Volume 46 , Issue 3 , November 1981 , pp. 469 - 480
Copyright
Copyright © The Nutrition Society 1981

References

Adams, K. F., Johnson, H. Jr., Hornowski, K. E. & Lineberger, T. H. (1979). Biochim. Biophys. Acta. 550, 279.CrossRefGoogle Scholar
Bremner, I. (1976). Proc. Nutr. Soc. 35, 21A.Google Scholar
Bremner, I. & Young, B. W. (1978). Br. J. Nutr. 39, 325.CrossRefGoogle Scholar
Dick, A. T. (1956). Soil Sci. 81, 229.CrossRefGoogle Scholar
Dick, A. T., Dewey, D. W. & Gawthorne, J. M. (1975). J. agric. Sci., Camb. 85, 567.CrossRefGoogle Scholar
El-Gallad, T. T., Bremner, I. & Mills, C. F. (1977). Proc. Nutr. Soc. 36, 105A.Google Scholar
Ford, E. J. H. (1967). J. Comp. Path. Ther. 77, 405.CrossRefGoogle Scholar
Froslie, A. & Norheim, G. (1976). Acta Vet. scand. 17, 307.CrossRefGoogle Scholar
Gaballah, S., Abood, L. G., Kapsalis, A. & Sturdivant, D. (1965). Proc. Soc. exp. Biol. Med. 119, 625.CrossRefGoogle Scholar
Goldberg, A., Williams, C. G., Jones, R. S., Yanagita, M., Cartwright, G. E. & Wintrobe, M. M. (1956). J. Lab. clin. Med. 48, 442.Google Scholar
Gooneratne, S. R. (1979). Morphometric, electron microscopic, biochemical and pathological investigation of liver of normal and copper poisoned sheep. Ph D Thesis, Murdoch University, Western Australia.Google Scholar
Gooneratne, S. R. & Howell, J. McC. (1980). Res. vet. Sci. 28, 351.CrossRefGoogle Scholar
Gooneratne, S. R., Howell, J. McC. & Gawthorne, J. M. (1981). Br. J. Nutr. 46, 457.CrossRefGoogle Scholar
Gubler, C. J., Lahey, M. E., Cartwright, G. E. & Wintrobe, M. M. (1953). J. clin. Invest. 32, 405.CrossRefGoogle Scholar
Hogan, K. G., Money, D. F. L. & Blayney, A. (1968). N. Z. Jl agric. Res. 11, 135.CrossRefGoogle Scholar
Howell, J. McC. (1978). In The Proceedings of the 3rd International Symposium on Trace Element Metabolism in Man and Animals, p. 536 [Kirchgessner, M., editor]. Weihenstephan: Arbeitskreis für Tierernahrungsforschung.Google Scholar
Howell, J. McC. & Gopinath, C. (1977). Res. vet. Sci. 22, 86.CrossRefGoogle Scholar
Ishmael, J. & Gopinath, C. (1972). J. Comp. Path. Ther. 82, 47.CrossRefGoogle Scholar
Ishmael, J., Gopinath, C. & Howell, J. McC. (1971). Res. vet. Sci. 12, 358.CrossRefGoogle Scholar
Ishmael, J., Gopinath, C. & Howell, J. McC. (1972). Res. vet. Sci. 13, 22.CrossRefGoogle Scholar
Kline, R. D., Hays, V. W. & Cromwell, G. L. (1971). J. Anim. Sci. 33, 771.CrossRefGoogle Scholar
Lessler, M. A. & Walters, M. J. (1973). Proc. Soc. exp. Biol. Med. 142, 548.CrossRefGoogle Scholar
McCosker, P. J. (1968). Res. vet. Sci. 9, 103.CrossRefGoogle Scholar
Marcilese, N. A., Ammerman, C. B., Valsecchi, R. M., Dunavant, B. G. & Davis, G. K. (1969). J. Nutr. 99, 177.CrossRefGoogle Scholar
Marcilese, N. A., Ammerman, C. G., Valsecchi, R. M., Dunavant, B. G. & Davis, G. K. (1970). J. Nutr. 100, 1399.CrossRefGoogle Scholar
Mills, C. F., Bremner, I., El-Gallad, T. T., Dalgarno, A. C. & Young, B. W. (1978). In The Proceedings of the 3rd International Symposium on Trace Element Metabolism in Man and Animals p. 150 [Kirchgessner, M., editor]. Weihenstephan: Arbeitskreis für Tierernahrungsforschung.Google Scholar
Ross, D. B. (1966). Br. vet. J. 122, 279.CrossRefGoogle Scholar
Rys, R. M., Kuklewicz, M. & Sokol, J. (1963). Roczn Naukro B 83, 145.Google Scholar
Schalm, O. W., Jain, N. C. & Carroll, E. J. (1975). In Veterinary Haematology, 3rd ed. p. 76. Philadelphia: Lea and Fabiger.Google Scholar
Smith, B. S. W., Field, A. C. & Suttle, N. F. (1968). J. Comp. Path. Ther. 78, 449.CrossRefGoogle Scholar
Smith, B. S. W. & Wright, H. (1974). Clinica chim. Acta 50, 359.CrossRefGoogle Scholar
Smith, B. S. W. & Wright, H. (1975 a). J. Comp. Path. Ther. 85, 299.CrossRefGoogle Scholar
Smith, B. S. W. & Wright, H. (1975 b). Clinica chim. Acta 62, 55.CrossRefGoogle Scholar
Suttle, N. F. (1974). Br. J. Nutr. 32, 395.CrossRefGoogle Scholar
Suttle, N. F. & Field, A. C. (1968). J. Comp. Path. 78, 351.CrossRefGoogle Scholar
Thompson, R. H. & Todd, J. R. (1976). Res. vet. Sci. 20, 257.CrossRefGoogle Scholar
Todd, J. R. (1969). Proc. Nutr. Soc. 28, 189.CrossRefGoogle Scholar
Todd, J. R. & Thompson, R. H. (1964). J. Comp. Path. Ther. 74, 542.CrossRefGoogle Scholar