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The activity of pepsin, chymotrypsin and trypsin during 24 h periods in the small intestine of growing pigs

Published online by Cambridge University Press:  09 March 2007

A. G. Low
A. G. Low
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading, Berks RG2 9AT
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Abstract

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1. Digesta were collected from twenty-two pigs, of 40 kg mean live weight, and fitted with single re-entrant cannulas in either the duodenum, jejunum or ileum.

2. Three approximately isonitrogenous diets were given to the pigs; their main constituents were: barley, fine wheat offal and white fish meal (diet BWF); starch, sucrose, maize oil, cellulose and either groundnut (diet SSG) or casein (diet SSC).

3. The activities of pepsin, chymotrypsin and trypsin were measured every hour in duodenal digesta during 24 h collection periods. Chymotrypsin and trypsin were also measured every hour in jejunal digesta and every 6 h in ileal digesta, during 24 h collection periods.

4. The mean total pepsin activities in the duodenal digesta during 24 h collection periods (units for a 40 kg pig given 1·7 kg diet) were: 7764400 (diet BWF), 6078400 (diet SSG), 5801 600 (diet SSC).

5. The mean total chymotrypsin activities (units for a 40 kg pig given 1·7 kg diet) in digesta in the duodenum, jejunum and ileum respectively were: 62920, 59560, 21 880 (diet BWF), 78240, 68400, 24680 (diet SSG). 75280, 76120, 6160 (diet SSC).

6. The mean total trypsin activities (units for a 40 kg pig given 1·7 kg diet) in digesta from the duodenum, jejunum and ileum, respectively were: 256840, 362840, 77600 (diet BWF), 211 200, 205280. 46720 (diet SSG) 325720, 428560, 13600 (diet SSC).

7. It was calculated that the total weights of pepsin, chymotrypsin and trypsin in duodenal digesta in 24 h periods were between 6·2 and 7·1 g. This represents 20–25% of previously published estimates of the amounts of endogenous protein in this part of the gut.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 48 , Issue 1 , July 1982 , pp. 147 - 159
Copyright
Copyright © The Nutrition Society 1982

References

REFERENCES

Anson, M. L. (1938). J. gen. Physiol. 22, 79.Google Scholar
Ash, R. W. (1962). Anim. Prod. 4, 309.Google Scholar
Barber, R. S., Braude, R., Mitchell, K. G. & Pittman, R. J. (1972). Anim. Prod. 14, 199.Google Scholar
Bovey, F. A. & Yanari, S. S. (1960). The Enzymes, 2nd ed. vol. 4, p. 63. New York: Academic Press.Google Scholar
Braude, R., Fulford, R. J. & Low, A. G. (1976). Br. J. Nutr. 36, 497.CrossRefGoogle Scholar
Buraczewska, L. (1980). In Current Concepts of Digestion and Absorption in Pigs, p. 151 [Low, A. G. and Partridge, I. G., editors]. Reading: National Institute for Research in Dairying.Google Scholar
Corring, T., Aumaitre, R. & Rerat, A. (1972). Annls Biol. anim. Biochim. Biophys. 12, 109.Google Scholar
Corring, T. & Saucier, R. (1972). Annls Biol anim. Biochim. Biophys. 12, 233.Google Scholar
Cranwell, P. D. (1977). Proc. Nutr. Soc. 36, 142A.Google Scholar
Cranwell, P. D. & Titchen, D. A. (1974). Res. Vet. Sci. 16, 105.CrossRefGoogle Scholar
Cranwell, P. D. & Titchen, D. A. (1976). Proc. Nutr. Soc. 35, 28A.Google Scholar
Cunningham, L. (1965). In Comprehensive Biochemistry, vol. 16, p. 85 [Florkin, M. and Stotz, E. H., editors]. Amsterdam: Elsevier.Google Scholar
Determan, H., Jaworek, D., Kotitschke, R. & Walch, A. (1969). Hoppe-Seyler's Z. physiol. Chem. 350, 379.CrossRefGoogle Scholar
Folk, J. E. & Schirmer, E. W. (1965). J. biol. Chem. 240, 181.CrossRefGoogle Scholar
Goldberg, D. M., Campbell, R. & Roy, A. D. (1969). Scand. J. Gastroenterology 4, 217.Google Scholar
Goldberg, D. M. & Wormsley, K. G. (1970). Gut 11, 859.CrossRefGoogle Scholar
Hohlacev, V. K. & Kasincev, B. S. (1962). Zap. Voronez. Sel'skohoz. Inst. Vet. Fak. 17, 225.Google Scholar
Höller, H. (1970). Zentbl. VetMed. A, 17, 658.Google Scholar
Hummel, B. C. W. (1959). Can. J. Biochem. Physiol. 37, 1303.CrossRefGoogle Scholar
Kowalewski, K., Secord, D. C. & Kolodej, A. (1974). Can. J. Surgery 17, 340.Google Scholar
Kvasnitskii, A. V. (1951). Voprosy fiziologii pishchevarenija u svinei (translated by Kidder, D. E.). Moscow: Sel'Khozgiz.Google Scholar
Lawrence, T. L. J. (1972). Br. vet. J. 128, 402.CrossRefGoogle Scholar
Low, A. G. (1979 a). Br. J. Nutr. 41, 137.CrossRefGoogle Scholar
Low, A. G. (1979 b). Br. J. Nutr. 41, 147.CrossRefGoogle Scholar
Low, A. G., Partridge, I. G. & Sambrook, I. E. (1978). Br. J. Nutr. 39, 515.CrossRefGoogle Scholar
Low, A. G. & Zebrowska, T. (1977). Br. J. Nutr. 38, 145.CrossRefGoogle Scholar
Maxwell, C. V., Reimann, E. M., Hoekstra, W. G., Kowalczyk, T., Benevenga, N. J. & Grummer, R. H. (1970). J. Anim. Sci. 30, 911.CrossRefGoogle Scholar
Partridge, I. G., Low, A. G., Sambrook, I. E. & Corring, T. (1982). Br. J. Nutr. 48, 137.CrossRefGoogle Scholar
Pekas, J. C., Thompson, A. M. & Hays, V. W. (1966). J. Anim. Sci. 25, 113.Google Scholar
Taylor, J. (1948). Nature, Lond. 162, 262.CrossRefGoogle Scholar
Tkachev, E. V., Smirnov, O. K., Pasechnik, A. P. & Pakhno, V. S. (1970). Sb. nauch. Rab. vses. nauchno-issled. Inst. Zhivot. 20, 28.Google Scholar
Zebrowska, T. & Buraczewska, L. (1972). Roczn. Nauk roln. Ser. B. 94, (1), 97.Google Scholar