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Abomasal glucose, maize starch and maize dextrin infusions in cattle: Small-intestinal disappearance, net portal glucose flux and ileal oligosaccharide flow

Published online by Cambridge University Press:  09 March 2007

K. K. Kreikemeier  and
D. L. Harmon
K. K. Kreikemeier
Affiliation:
U.S. Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska 68933, USA
D. L. Harmon
Affiliation:
Department of Animal Sciences, University of Kentucky, Lexington, 40546-1027, Kentucky, USA
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Abstract

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Three castrated male Holstein cattle (423 (SD 19) kg live weight) fitted with elevated carotid artery, portal, and mesenteric venous catheters, and abomasal and ileal cannulas were used to study small-intestinal starch digestion. The cattle were infused abomasally with water (275 ml/h), glucose (66 g/h), maize dextrin (66 g/h) or maize starch (66 g/h) in an incomplete Latin square design, with eight infusion periods. Infusion with carbohydrate resulted in higher arterial glucose concentrations and greater net portal glucose flux than when cattle were infused with water. Arterial glucose concentration and net portal glucose flux were highest when glucose was infused. In the small intestine, 85% of abomasally infused glucose, 78% of infused dextrin, and 66% of infused starch disappeared. Of the carbohydrate that disappeared in the small intestine, that which could be accounted for as net portal glucose flux was 73% for glucose, 60% for dextrin, and 57% for starch. Ileal digesta contained unpolymerized glucose, and short-chain soluble α-glucoside. Of the infused dextrin flowing past the ileum (14 g/h), 0·3 g/h was glucose, 6·2 g/h was soluble α-glucoside, and 7·5 g/h was insoluble α-glucoside. Of the infused starch flowing at the ileum (22·2 g/h), 0-9 g/h was glucose, 5·3 g/h was soluble α-glucoside, and 15·9 g/h was insoluble α-glucoside. The average chain lengths of the soluble α-glucosides in ileal digesta were 2·07 and 2·36 for dextrin and starch infusions respectively, indicating mostly di- and to a lesser extent trisaccharides. We conclude that (1) when 66 g raw starch is presented to the small intestine per h, about half of the intestinal disappearance appears as glucose in the portal vasculature, and (2) α-1,4 glucosidase (EC 3.2.1.20) activity at the brush border is the rate-limiting step to small-intestinal starch digestion in cattle.

Keywords

CattleStarch digestionSmall intestine
Type
Abomasal carbohydrate infusions in cattle
Information
British Journal of Nutrition , Volume 73 , Issue 5 , May 1995 , pp. 763 - 772
Copyright
Copyright © The Nutrition Society 1995

References

Andrieux, C., Pacheco, E. D., Bouchet, B., Gallant, D. & Szylit, O. (1992). Contribution of the digestive tract microflora to amylomaize starch degradation in the rat. British Journal of Nutrition 67, 489–499.Google Scholar
Axe, D. E., Bolson, K. K., Harmon, D. L., Lee, R. W., Milliken, G. A. & Avery, T. B. (1987). Effect of wheat and high moisture sorghum grain fed singly and in combination on ruminal fermentation, solid and liquid flow, site and extent of digestion and feeding performance of cattle. Journal of Animal Science 64, 897–906.Google Scholar
Binnerts, W. T., Van't Klooster, A. T. & Frens, A. M. (1968). Soluble chromium indicator measured by atomic absorption in digestion experiments. Veterinary Record 81, 470.Google Scholar
Cappelli, F. P., Seal, C. J. & Parker, D. S. (1993). Portal glucose absorption and utilization in sheep receiving exogenous glucose intravascularly or intraduodenally. Journal of Animal Science 70, Suppl. 1, 279 (Abstr.).Google Scholar
Gochman, N. & Schmitz, J. M. (1972). Application of a new peroxide indicator reaction to the specific automated determination of glucose with glucose oxidase. Clinical Chemistry 18, 943–950.Google Scholar
Gray, G. M. (1975). Oligosaccharidases of the small intestinal brush border. In Physiological Effects of Food Carbohydrates, pp. 181–190 [Allene, J. and Hodge, J., editors]. Washington D.C.: American Chemical Society.CrossRefGoogle Scholar
Harmon, D. L., Avery, T. B., Huntington, G. B. & Reynolds, P. J. (1988). Influence of ionophore addition to roughage and high-concentrate diets on portal blood flow and net nutrient flux in cattle. Canadian Journal of Animal Science 68, 419.CrossRefGoogle Scholar
Harvey, R. B. & Brothers, A. J. (1962). Renal extraction of para-aminohippurate and creatinine measured by continuous in vivo sampling of arterial and renal-vein blood. Annals of the New York Academy of Sciences 102, 46–54.Google Scholar
Kennedy, H. M. & Fischer, A. C. Jr (1984). Starch and dextrins in prepared adhesives. In Starch: Chemistry and Technology, 2nd ed., pp. 593–610 [Whistler, R. L., BeMiller, J. N. and Paschall, E. F., editors]. Orlando, Florida: Academic Press.CrossRefGoogle Scholar
Kidder, D. E., Hill, F. W. G. & Stevens, J. A. (1972). Automatic measurement of some mucosal carbohydrases. Clinica Chimica Acta 37, 491–501.Google Scholar
Krehbiel, C. & Britton, R. (1993). Glucose absorption in the small intestine in steers. Nebraska Beef Cattle Report MP: 59-A, 66–67.Google Scholar
Kreikemeier, K. K., Harmon, D. L., Brandt, R. T. Jr, Avery, T. B. & Johnson, D. E. (1991). Small intestinal starch digestion in steers: effect of various levels of abomasal glucose, corn starch and corn dextrin infusion on small intestinal disappearance and net glucose absorption. Journal of Animal Science 69, 328–338.CrossRefGoogle ScholarPubMed
Kreikemeier, K. K., Harmon, D. L. & Nelssen, J. L. (1990 a). Influence of hydrocortisone acetate on pancreas and mucosal weight, amylase and disaccharidase activities in 14-day-old pigs. Comparative Biochemistry and Physiology 97A, 45–50.Google Scholar
Kreikemeier, K. K., Harmon, D. L., Peters, J. P., Gross, K. L., Armendariz, C. K. & Krehbiel, C. R. (1990 b). Influence of dietary forage and feed intake on carbohydrase activities and small intestinal morphology of calves. Journal of Animal Science 68, 2916–2929.Google Scholar
MacRae, J. C. & Armstrong, D. G. (1968). Enzyme methods for determination of alpha-linked glucose polymers in biological materials. Journal of the Science of Food and Agriculture 19, 578–581.CrossRefGoogle Scholar
Mayes, R. W. & Ørskov, E. R. (1974). The utilization of gelled maize starch in the small intestine of sheep. British Journal of Nutrition 32, 143–153.Google Scholar
Poore, M. H., Moore, J. A., Eck, T. P., Swingle, R. S. & Theurer, C. B. (1993). Effect of fiber source and ruminal starch degradability on site and extent of digestion in dairy cows. Journal of Dairy Science 76, 2244–2253.CrossRefGoogle Scholar
Roberts, P. J. P. & Whelan, W. J. (1960). The mechanism of carbohydrase action. 5. Action of human salivary alpha-amylase on amylopectin and glycogen. Biochemical Journal 76, 246–253.CrossRefGoogle ScholarPubMed
Robyt, J. F. (1984). Enzymes in the hydrolysis and synthesis of starch. In Starch: Chemistry and Technology, 2nd ed., pp. 87–123 [Whistler, R. L., Be Miller, J. N. and Paschall, E. F. editors]. Orlando, Florida: Academic Press.Google Scholar
Robyt, J. F. & French, D. (1967). Multiple attack hypothesis of alpha-amylase action: action of porcine pancreatic, human salivary, and aspergillus oryzae alpha-amylases. Archives of Biochemistry and Biophysics 122, 8–16.Google Scholar
SAS (1985). SAS Users' Guide: Statistics. Cary, NC: SAS Institute Inc.Google Scholar
Seal, C. J., Parker, D. S. & Lobley, G. E. (1993). Effect of intraduodenal starch infusion on glucose metabolism of growing steers fed alfalfa. Journal of Animal Science 70, Suppl. 1, 279 (Abstr.).Google Scholar
Streeter, M. N., Wagner, D. G., Hibberd, C. A. & Owens, F. N. (1990 a). The effect of sorghum grain variety on site and extent of digestion in beef heifers. Journal of Animal Science 68, 1121–1132.Google Scholar
Streeter, M. N., Wagner, D. G., Hibberd, C. A. & Owens, F. N. (1990 b). Comparison of corn with four sorghum grain hybrids: site and extent of digestion in steers. Journal of Animal Science 68, 3429–3440.Google Scholar
Streeter, M. N., Wagner, D. G., Owens, F. N. & Hibberd, C. A. (1989). Combinations of high-moisture harvested sorghum grain and dry-rolled corn: effects on site and extent of digestion in beef heifers. Journal of Animal Science 67, 1623–1633.Google Scholar
Streeter, M. N., Wagner, D. G., Owens, F. N. & Hibberd, C. A. (1991). The effect of pure and partial yellow endosperm sorghum grain hybrids on site and extent of digestion in beef steers. Journal of Animal Science 69, 2571–2584.CrossRefGoogle ScholarPubMed
Taravel, F. R., Datema, R., Woloszczuk, W., Marshall, J. J. & Whelan, W. J. (1983). Purification and characterization of a pig intestinal α-limit dextrinase. European Journal of Biochemistry 130, 147–153.Google Scholar
Zinn, R. A. (1990). Influence of steaming time on site of digestion of flaked corn in steers. Journal of Animal Science 68, 776–781.Google Scholar