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Yeasts from the Maritime Antarctic: tools for industry and bioremediation

Published online by Cambridge University Press:  20 October 2021

Brenda Bezus Open the ORCID record for Brenda Bezus [Opens in a new window] ,
Gabriela Garmendia Open the ORCID record for Gabriela Garmendia [Opens in a new window] ,
Silvana Vero Open the ORCID record for Silvana Vero [Opens in a new window] ,
Sebastián Cavalitto Open the ORCID record for Sebastián Cavalitto [Opens in a new window]  and
Ivana Alejandra Cavello Open the ORCID record for Ivana Alejandra Cavello [Opens in a new window]
Brenda Bezus
Affiliation:
Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI, UNLP; CCT-La Plata, CONICET), Calle 47 y 115, B1900ASH, La Plata, Provincia de Buenos Aires, Argentina
Gabriela Garmendia
Affiliation:
Cátedra de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo (11800), Uruguay
Silvana Vero
Affiliation:
Cátedra de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo (11800), Uruguay
Sebastián Cavalitto
Affiliation:
Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI, UNLP; CCT-La Plata, CONICET), Calle 47 y 115, B1900ASH, La Plata, Provincia de Buenos Aires, Argentina
Ivana Alejandra Cavello*
Affiliation:
Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI, UNLP; CCT-La Plata, CONICET), Calle 47 y 115, B1900ASH, La Plata, Provincia de Buenos Aires, Argentina
*
icavello@biotec.quimica.unlp.edu.ar
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Abstract

We isolated 32 yeasts from King George Island, which we then identified and characterized. Twenty-six belonged to Basidiomycota among the genera Naganishia, Holtermaniella, Vishniacozyma, Phenoliferia, Mrakia and Cystobasidium, and only six were Ascomycota of the genera Metschnikowia and Debaryomyces. Thirteen were psychrophiles, while 19 were psychrotolerant. Certain isolates exhibited a high tolerance to NaCl (3.5 M), while most tolerated Ni2+, Zn2+ and Li+. Cu2+ and Cd2+, however, inhibited the growth of most of the isolates. We assessed a bioprospecting of extracellular enzymes and their ability to biodegrade or bioaccumulate textile dyes. β-Glucosidases (59%) and esterases (53%) were the main extracellular enzymes detected. A minor proportion of the yeasts produced pectinases and xylanases; only psychrophiles produced proteases. Vishniacozyma, Naganishia, Phenoliferia and Mrakia were the richest genera in terms of enzyme production. Greater than 70% of the isolates decolourized solid medium supplemented with various dyes at 4°C and 20°C. Isolates belonging to the genera Vishniacozyma, Cystobasidium, Mrakia and Phenoliferia seem to have potential for textile dye bio-decolourization. The results demonstrated that yeasts collected from the Maritime Antarctic are a potential source of new enzymes of biotechnological interest, and that certain isolates could potentially be considered in the design of textile wastewater decolourizations.

Keywords

Antarctic yeastsbiotechnologycold-active enzymescold-adapted yeaststextile-dye decolourization
Type
Biological Sciences
Information
Antarctic Science , Volume 34 , Issue 1 , February 2022 , pp. 16 - 28
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Copyright
Copyright © Antarctic Science Ltd 2021

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