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A protocol for the cryoconservation of breeds by low-cost emergency cell banks – a pilot study

Published online by Cambridge University Press:  01 January 2008

E. Groeneveld ,
Nguyen Huu Tinh ,
W. Kues  and
Nguyen Thi Vien
E. Groeneveld*
Affiliation:
Institute for Animal Breeding, Federal Agricultural Research Centre, Mariensee, Germany
Nguyen Huu Tinh
Affiliation:
Department of Animal Genetics and Breeding, Institute of Agricultural Sciences of South Vietnam, Ho Chi Minh City, Vietnam
W. Kues
Affiliation:
Institute for Animal Breeding, Federal Agricultural Research Centre, Mariensee, Germany
Nguyen Thi Vien
Affiliation:
Department of Animal Genetics and Breeding, Institute of Agricultural Sciences of South Vietnam, Ho Chi Minh City, Vietnam
*
E-mail: eildert.groeneveld@fal.de
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Abstract

A protocol was developed for collection and storage of somatic cell samples under adverse conditions with little infrastructure, for somatic-cell banks as a backup for endangered livestock breeds. The procedure, which is uniform across species, includes sample collection with ear taggers with an integrated tag/vial system, and recording of global positioning system (GPS) coordinates, digital images and breed information. In a pilot study, the procedures were tested on six local Vietnamese populations of pigs, sheep and goats. Initial investment was around €3000, while the total variable cost for sampling one breed with 25 females and 25 males was less than €1000. With support from local organisations, the sampling of six breeds with 300 animals was carried out in 2 months. The protocol and the complete workflow for setting up a somatic-cell bank, together with data collection, are described. The procedure has proved practicable and exceedingly cheap relative to the cryopreservation of semen or embryos.

Keywords

biodiversitydatabasesendangered breedsgene bankssomatic cells
Type
Full Paper
Information
animal , Volume 2 , Issue 1 , January 2008 , pp. 1 - 8
Copyright
Copyright © The Animal Consortium 2008

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