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Postnatal differential expression of chemoreceptors of free fatty acids along the gastrointestinal tract of supplemental feeding v. grazing kid goats

Published online by Cambridge University Press:  10 July 2018

T. Ran ,
Y. Liu ,
J. Z. Jiao ,
C. S. Zhou ,
S. X. Tang ,
M. Wang ,
Z. X. He ,
Z. L. Tan ,
W. Z. Yang  and
K. A. Beauchemin
T. Ran
Affiliation:
Key Laboratory for Agro-Ecological Processes in Subtropical Region, and National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P.R. China Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta T1J 4B1, Canada
Y. Liu
Affiliation:
Key Laboratory for Agro-Ecological Processes in Subtropical Region, and National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P.R. China
J. Z. Jiao
Affiliation:
Key Laboratory for Agro-Ecological Processes in Subtropical Region, and National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P.R. China
C. S. Zhou*
Affiliation:
Key Laboratory for Agro-Ecological Processes in Subtropical Region, and National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P.R. China Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha, Hunan 410128, P.R. China
S. X. Tang
Affiliation:
Key Laboratory for Agro-Ecological Processes in Subtropical Region, and National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P.R. China Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha, Hunan 410128, P.R. China
M. Wang
Affiliation:
Key Laboratory for Agro-Ecological Processes in Subtropical Region, and National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P.R. China Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha, Hunan 410128, P.R. China
Z. X. He
Affiliation:
Key Laboratory for Agro-Ecological Processes in Subtropical Region, and National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P.R. China
Z. L. Tan
Affiliation:
Key Laboratory for Agro-Ecological Processes in Subtropical Region, and National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P.R. China Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha, Hunan 410128, P.R. China
W. Z. Yang
Affiliation:
Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta T1J 4B1, Canada
K. A. Beauchemin
Affiliation:
Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta T1J 4B1, Canada
*
†E-mail: zcs@isa.ac.cn
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Abstract

The gastrointestinal tract (GIT) of animals is capable of sensing various kinds of nutrients via G-protein coupled receptor-mediated signaling transduction pathways, and the process is known as ‘gut nutrient chemosensing’. GPR40, GPR41, GPR43 and GPR119 are chemoreceptors for free fatty acids (FFAs) and lipid derivatives, but they are not well studied in small ruminants. The objective of this study is to determine the expression of GPR40, GPR41, GPR43 and GPR119 along the GIT of kid goats under supplemental feeding (S) v. grazing (G) during early development. In total, 44 kid goats (initial weight 1.35±0.12 kg) were slaughtered for sampling (rumen, abomasum, duodenum, jejunum, ileum, cecum, colon and rectum) between days 0 and 70. The expression of GPR41 and GPR43 were measured at both mRNA and protein levels, whereas GPR40 and GPR119 were assayed at protein level only. The effects of age and feeding system on their expression were variable depending upon GIT segments, chemoreceptors and expression level (mRNA or protein), and sometimes feeding system × age interactions (P<0.05) were observed. Supplemental feeding enhanced expression of GPR40, GPR41 and GPR43 in most segments of the GIT of goats, whereas G enhanced expression of GPR119. GPR41 and GPR43 were mainly expressed in rumen, abomasum and cecum, with different responses to age and feeding system. GPR41 and GPR43 expression in abomasum at mRNA level was greatly (P<0.01) affected by both age and feeding system; whereas their expression in rumen and abomasum at protein level were different, feeding system greatly (P<0.05) affected GPR41 expression, but had no effect (P>0.05) on GPR43 expression; and there were no feeding system×age interactions (P>0.05) on GPR41 and GPR43 protein expression. The expression of GPR41 and GPR43 in rumen and abomasum linearly (P<0.01) increased with increasing age (from days 0 to 70). Meanwhile, age was the main factor affecting GPR40 expression throughout the GIT. These outcomes indicate that age and feeding system are the two factors affecting chemoreceptors for FFAs and lipid derivatives expression in the GIT of kids goats, and S enhanced the expression of chemoreceptors for FFAs, whereas G gave rise to greater expression of chemoreceptors for lipid derivatives. Our results suggest that enhanced expression of chemoreceptors for FFAs might be one of the benefits of early supplemental feeding offered to young ruminants during early development.

Keywords

short-chain fatty acidsgut nutrient chemosensingfeeding system developmental changesgoat
Type
Research Article
Information
animal , Volume 13 , Issue 3 , March 2019 , pp. 509 - 517
Copyright
© The Animal Consortium 2018 

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