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Polyethylene glycol marker measured with NIRS gives a reliable estimate of the rangeland intake of grazing sheep

Published online by Cambridge University Press:  01 December 2015

P. Hassoun ,
D. Bastianelli ,
D. Foulquié ,
L. Bonnal  and
F. Bocquier
P. Hassoun*
Affiliation:
Institut National de la Recherche Agronomique, UMR868 Systèmes d’élevage méditerranéens et tropicaux, F-34060 Montpellier, France Montpellier SupAgro, F-34060 Montpellier, France Centre de coopération International en Recherche Agronomique pour le Développement, F-34980 Montpellier, France
D. Bastianelli
Affiliation:
Institut National de la Recherche Agronomique, UMR868 Systèmes d’élevage méditerranéens et tropicaux, F-34060 Montpellier, France Montpellier SupAgro, F-34060 Montpellier, France Centre de coopération International en Recherche Agronomique pour le Développement, F-34980 Montpellier, France
D. Foulquié
Affiliation:
Institut National de la Recherche Agronomique, UE0321 Domaine de La Fage, F-12250 Saint Jean et Saint Paul, France
L. Bonnal
Affiliation:
Institut National de la Recherche Agronomique, UMR868 Systèmes d’élevage méditerranéens et tropicaux, F-34060 Montpellier, France Montpellier SupAgro, F-34060 Montpellier, France Centre de coopération International en Recherche Agronomique pour le Développement, F-34980 Montpellier, France
F. Bocquier
Affiliation:
Institut National de la Recherche Agronomique, UMR868 Systèmes d’élevage méditerranéens et tropicaux, F-34060 Montpellier, France Montpellier SupAgro, F-34060 Montpellier, France Centre de coopération International en Recherche Agronomique pour le Développement, F-34980 Montpellier, France
*
†E-mail: philippe.hassoun@supagro.inra.fr
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Abstract

Polyethylene glycol (PEG) measured with NIRS is known to be a valuable faecal marker when used in indoor experiments. In order to verify whether it can be used at pasture, an experiment was conducted with two trials. In trial 1, six Romane breed adult dry ewes placed in metabolism cages were fed daily with natural, freshly cut rangeland from a fertilised or unfertilised paddock for 6 weeks. Three ewes did not receive PEG and the three others were dosed with 10 g of PEG in solution form once daily until the end of the experiment to measure in vivo dry matter digestibility and PEG recovery rate for each forage quality. At the same time (trial 2), 15 ewe lambs and 14 lactating adult ewes suckling one or two lambs were allowed to graze together on the same herbage as that cut for indoor ewes. All animals were initially equipped with faecal bags emptied twice daily for collecting total faeces, and eight ewe lambs and seven adults were dosed once daily with 10 g of PEG. Faecal grab samples were collected for 4 to 5 days for each forage quality grazed. Indoor trial 1 showed that PEG had no effect on dry matter intake (DMI) or on digestibility. PEG recovery rates measured on fertilised (77.7%) and unfertilised (82.1%) forage were not different (P>0.05). PEG recovery rates measured at pasture did not differ (P>0.05) between pasture quality and animal type with an average value of 68.9%. Faecal output measured with bags or estimated with PEG and calculated DMI were not different (P>0.05) when PEG recovery rate measured at pasture was used. Conversely, using indoor PEG recovery values, significantly (P<0.05) or tended to overestimate faecal output. In conclusion, PEG could be used as a faecal marker administered at a minimal dose of 1% of DMI with a recovery rate measured under pasture conditions for pasture intake measurements on a group of animals at the same physiological stage but not for individual measurements.

Keywords

sheeprangelandintakedigestibilitypolyethylene glycol
Type
Research Article
Information
animal , Volume 10 , Issue 5 , May 2016 , pp. 771 - 778
Copyright
© The Animal Consortium 2015 

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References

Andueza, D, Picard, F, Aufrère, J, Jamot, J, Béchet, G and Baumont, R 2013. Polyethylene glycol determined by near-infrared reflectance spectroscopy to estimate faecal output in sheep fed fresh permanent grassland forage. Livestock Production Science 155, 38–43.CrossRefGoogle Scholar
Andueza, D, Picard, F, Pradel, P, Egal, D, Hassoun, P, Peccatte, JR and Baumont, R 2011. Reproducibility and repeatability of forage in vivo digestibility and voluntary intake of permanent grassland forages in sheep. Livestock Science 140, 42–48.Google Scholar
Aufrère, J, Baumont, R, Delaby, L, Peccatte, JR, Andrieu, J, Andrieu, J-P and Dulphy, J-P 2007. Prévision de la digestibilité des fourrages par la méthode pepsine-cellulase. Le point sur les équations proposées. INRA Productions Animales 20, 129–136.Google Scholar
Barry, TN and McNabb, WC 1999. The implications of condensed tannins on the nutritive value of temperate forages fed to ruminants. British Journal of Nutrition 81, 263–272.Google Scholar
Beaumont, R, Champciaux, P, Agabriel, J, Andrieu, J, Aufrère, J, Michalet-Doreau, B and Demarquilly, C 1999. Une démarche intégrée pour prévoir la valeur des aliments pour les ruminants: PrévAlim pour INRAtion. INRA Productions Animales 12, 183–194.Google Scholar
Benvenutti, MA, Coates, DB, Bindelle, J, Poppi, DP and Gordon, IJ 2014. Can faecal markers detect a short term reduction in forage intake by cattle? Animal Feed Science and Technology 194, 44–57.CrossRefGoogle Scholar
Burns, RE 1971. Methods for estimation of tannin in grain sorghum. Agronomy Journal 63, 511–512.Google Scholar
Caja, G, Ralha, VM and Albanell, E 2009. Evaluacion del polietilenglicol (PEG6000) como marcador indigestible para ovejas lecheras en estabulacion o pastoreo. In XXXIX Jornadas sobre Produccion Animal (ed. Associacion Interprofesional para el Desarollo Agrario), pp. 358–360. Zaragoza, Spain.Google Scholar
Casasus, I and Albanell, E 2014. Prediction of faecal output and hay intake by cattle from NIRS estimates of faecal concentrations of orally-dosed polyethyleneglycol. Animal Feed Science and Technology 192, 48–61.CrossRefGoogle Scholar
Chollet, S, Rambal, S, Fayolle, A, Hubert, D, Foulquié, D and Garnier, E 2014. Combined effects of climate, resource availability and plant traits on biomass produced in a Mediterranean rangeland. Ecology 95, 737–748.CrossRefGoogle Scholar
Coombe, JB and Kay, RNB 1965. Passage of digesta through the intestines of the sheep. Retention times in the small and large intestines. British Journal of Nutrition 19, 325–338.Google Scholar
Corbett, JL 1978. Measuring animal performance. In Measurement of grassland vegetation and animal production (ed. L 't Mannetje), pp. 163–230. CAB Publishing, Hurley, UK.Google Scholar
Corbett, JL, Greenhalgh, JFD, Gwynn, PE and Walker, D 1958. Excretion of chromium sesquioxide and polyethylene glycol by dairy cows. British Journal of Nutrition 12, 266–276.Google Scholar
Curtis, KMS, Holst, PJ and Murray, PJ 1994. Measuring supplement intake in the field using ytterbium as a marker. Australian Journal of Experimental Agriculture 34, 339–343.Google Scholar
Dove, H and Mayes, RW 1991. The use of plant wax alkanes as marker substances in studies of the nutrition of herbivores: a review. Australian Journal of Agricultural Research 42, 913–952.CrossRefGoogle Scholar
Doyle, PT, Casson, T, Cranberg, L and Rowe, JB 1994. Faecal output of grazing sheep measured by total collection or using chromium sesquioxide. Small Ruminant Research 13, 231–236.Google Scholar
Goering, HK and Van Soest, PJ 1970. Forage fiber analyses (apparatus, reagents, procedures and some applications). Agriculture Handbook No. 379. Agriculture Research Service, USDA, Washington, DC, USA.Google Scholar
Hassoun, P, Bastianelli, D, Autran, P and Bocquier, F 2014. Polyethylene glycol compared to ytterbium oxide as a total faecal output marker to predict organic matter intake of dairy ewes fed indoors or at pasture. Animal 8, 1420–1426.Google Scholar
Hassoun, P, Bastianelli, D and Bonnal, L 2007. Determination of polyethylene glycol (PEG) concentration in sheep faeces with near infrared spectroscopy. In Proceedings of the 12th International Conference – Near Infrared Spectroscopy. Auckland, New Zealand, 9–15 April 2005 (ed. GR Burling-Claridge, SE Holroyd and RMW Sumner), pp. 34–36. NZ NIRS Society, Inc., Hamilton, New Zealand.Google Scholar
Hassoun, P and Bocquier, F 2010. Alimentation des ovins. In Alimentation des bovins, ovins et caprins. Besoins des animaux - Valeurs des aliments - Tables Inra 2007 mise à jour 2010 (ed. Quae), pp. 121–136. Quae c/o Inra, Versailles, France.Google Scholar
Hassoun, P, Viudes, G, Autran, P, Bastianelli, D and Bocquier, F 2013. A method for estimating dry forage intake by sheep using polyethylene glycol as a faecal marker measured with NIRS. Animal 7, 1280–1288.Google Scholar
Hopson, DE and McCroskey, JE 1972. Influence of dose level and method of administration on the use of polyethylene glycol for determining fecal output of cattle. Journal of Animal Science 35, 1054–1057.Google Scholar
Ingleton, JW 1971. Faeces collection in young male lambs and wether sheep. Journal of the British Grassland Society 26, 103–106.Google Scholar
Landau, S, Friedman, S, Devash, L and Mabjeesh, S 2002. Polyethylene glycol, determined by near-infrared reflectance spectroscopy, as a marker of fecal output in goats. Journal of Agricultural and Food Chemistry 50, 1374–1378.Google Scholar
Landau, S, Xue, B, Dvash, L, Friedman, S and Mabjeesh, SJ 2003. Polyethylene glycol, used to alleviate the negative effects of dietary tannins, can also serve as a marker of fecal output in goats. Small Ruminant Research 48, 37–43.Google Scholar
Makkar, HPS, Blummel, L and Becker, K 1995. Formation of complexes between polyvinyl pyrrolidones or polyethylene glycols and tannins and their implication in gas production and true digestibility in in vitro techniques. British Journal of Nutrition 73, 897–913.Google Scholar
Molénat, G, Foulquié, D, Autran, P, Bouix, J, Hubert, D, Jacquin, M, Bocquier, F and Bibé, B 2005. Pour un élevage ovin allaitant performant et durable sur parcours: un système expérimental sur le Causse du Larzac. INRA Productions Animales 18, 323–338.Google Scholar
Official Journal of the European Union 2003. Commission Directive 2003/95/EC of 27 October 2003 amending Directive 96/77/EC laying down specific purity criteria on food additives other than colours and sweeteners, Official Journal of the European Union. 46, 71–77. Retrieved September 29, 2015, from http://eur-lex.europa.eu/legal-content/EN/TXT/?uri=uriserv:OJ.L_.2003.283.01.0071.01.ENG.Google Scholar
Raymond, WF and Minson, DJ 1955. The use of chromic oxide for estimating the faecal production of grazing animals. Journal of the British Grassland Society 10, 282–296.Google Scholar
Scherrer, B 1984. Les comparaisons de moyennes. In Biostatistique (ed. G Morin), pp. 401–463. G Morin, Paris, France.Google Scholar
Schiller, LR, Emmett, M, Santa Ana, CA and Fordtran, JS 1988. Osmotic effects of polyethylene glycol. Gastroenterology 94, 933–941.Google Scholar
Sprent, P 1992. Pratique des statistiques non paramétriques. INRA, Paris, France.Google Scholar
Teeter, RG and Owens, FN 1981. The potential use of five water soluble markers for measuring rumen liquid volume and dilution rate. Journal of Animal Science 53, 436.Google Scholar
Teeter, RG and Owens, FN 1983. Characteristics of water soluble markers for measuring rumen liquid volume and dilution rate. Journal of Animal Science 56, 717–728.Google Scholar