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Once-a-day milking of Holstein and Montbéliarde cows for 7 weeks in mid-lactation

  • D. Pomiès (a1), B. Martin (a1), Y. Chilliard (a1), P. Pradel (a2) and B. Rémond (a3)...


This study aimed at comparing the effects of once-a-day (OAD) milking during the descending phase of lactation between cows from the two most common breeds in France (Holstein and Montbéliarde). This study was carried on during two successive summers on a total of 50 Holstein and 38 Montbéliarde cows. During 7 weeks, half of the cows from each breed was milked OAD while the other half was milked twice a day (TAD). The animals were also followed for the next 3 or 5 weeks when they were all milked TAD, to check for any residual effect of OAD milking. The behaviour of OAD cows was observed around milking time. The incidence of diseases, the main performance variables (milk production, milk flow rate, live weight and body condition score), the detailed composition of milk (fat, protein, lactose, somatic cells, minerals, pH, free fatty acid (FFA), nitrogen fractions and enzymes) and some technological variables (clotting time and curd firmness) were measured on all cows.

Some signs of disturbance were observed in the OAD cows at the time when milking was omitted: some cows mooed, some went close to the exit of the paddock, some leaked milk prior to milking. However, these signs disappeared after 2 days. After the experimental period, the live weight and the body condition score of TAD and OAD cows did not differ significantly. OAD cows produced 4.5 kg/day less than TAD (P < 0.001), this being more marked in Holstein (5.7 kg/day, P < 0.001) than Montbéliarde (3.3 kg/day, P < 0.001) cows. The milk contents of fat, whey protein, casein, total protein and phosphorus, and its plasminogen activity, were higher with OAD cows while lactose and FFA contents, and lipoprotein lipase activity were lower, with no interaction observed with breed. During the subsequent 3 weeks, when all cows were again milked TAD, OAD cows still produced 1.7 kg/day less milk (P < 0.01) with slightly higher fat and protein content.

OAD milking for 7 weeks during the descending phase of lactation decreased milk production but increased milk content of most components, with a low residual effect. Montbéliarde cows were less affected by OAD milking than Holstein cows.


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Akpa, GN, Osuhor, CU, Olugbemi, TS, Nwani, PI 2003. Milk flow rate and milking frequency in Red Sokoto goats. Pakistan Journal of Nutrition 2, 192195.
Brulé A, Brocard V, Portier B and Racine V 2003. Effets de la réduction de la fréquence de traite sur le bien être de la vache laitière. In Proceedings of 10e Rencontres autour des Recherches sur les Ruminants, Paris, France, pp. 77–80.
Brunschwig P and Lamy JM 2004. Effets d’une traite par jour pendant 2 mois et du niveau protéique de la ration sur des vaches laitières en milieu de lactation. In Proceedings of 11e Rencontres autour des Recherches sur les Ruminants, Paris, France, p. 229.
Bugaud, C, Buchin, S, Coulon, JB, Hauwuy, A, Dupont, D 2001. Influence of the nature of alpine pastures on plasmin activity, fatty acid and volatile compound composition of milk. Lait 81, 401414.
Carruthers VR, Davis SR, Bryant AM and Morris CA 1989. Selection of cows for once a day milking. In Proceedings of the Ruakura Farmer’s Conference, pp. 12–14.
Carruthers, VR, Davis, SR, Bryant, AM, Henderson, HV, Morris, CA, Copeman, PJA 1993. Response of Jersey and Friesian cows to once a day milking and prediction of response based on udder characteristics and milk composition. Journal of Dairy Research 60, 111.
Chazal, MP, Chilliard, Y 1987. Effect of breed of cow (Friesian and Montbeliarde) on spontaneous and induced lipolysis in milk. Journal of Dairy Research 54, 711.
Chilliard, Y, Lamberet, G 1984. La lipolyse dans le lait: les différents types, mécanismes, facteurs de variation, signification pratique. Lait 64, 544578.
Claesson, O, Hansson, A, Gustafsson, N, Brännäng, E 1959. Studies on monozygous cattle twins. XVII. Once-a-day milking compared with twice-a-day milking. Acta Agriculturæ Scandinavica 8, 3858.
Clark, DA, Phyn, CVC, Tong, MJ, Collis, SJ, Dalley, DE 2006. A systems comparison of once- versus twice-daily milking of pastured dairy cows. Journal of Dairy Science 89, 18541862.
Dalgleish, DG 1993. The enzymatic coagulation of milk. In Cheese: chemistry, physics and microbiology. Volume 1. General aspects (ed. PF Fox, T McSweeney, T cogan and T Guinee), pp. 69100. Chapman and Hall Ltd., London, UK.
Davis, SR, Farr, VC, Stelwagen, K 1998. Once-daily milking of dairy cows: an appraisal. Proceedings of the New Zealand Society of Animal Production 58, 3640.
Davis, SR, Farr, VC, Stelwagen, K 1999. Regulation of yield loss and milk composition during once-daily milking: a review. Livestock Production Science 59, 7794.
Faulconnier, Y, Thevenet, M, Flechet, J, Chilliard, Y 1994. Lipoprotein lipase and metabolic activities in incubated bovine adipose tissue explants: effects of insulin, dexamethasone, and fetal bovine serum. Journal of Animal Science 72, 184191.
Ferris CP, Mayne CS, Frost JP, Binnie RC and McCoy MA 2003. A comparison of once- v. twice-daily milking on performance of late lactation dairy cows. In Proceedings of the British Society of Animal Science 2003, p. 25.
Guéguen, L, Pomiès, D, Rémond, B 2004. Ne plus traire qu’une fois par jour. In Traite et travail – Quelles solutions pour réduire l’astreinte liée à la traite? (ed. Institut de l’Elevage), pp. 1926. Institut de l’Elevage, Paris, France.
Holmes, CW, Wilson, GF, Mackenzie, DDS, Purchas, J 1992. The effects of milking once daily throughout lactation on the performance of dairy cows grazing on pasture. Proceedings of the New Zealand Society of Animal Production 52, 1316.
Holmes, CW, Kamote, H, Mackenzie, DDS, Morel, PCH 1996. Effects of a decrease in milk yield, caused by once-daily milking or by restricted feeding, on the somatic cell count in milk from cows with or without subclinical mastitis. Australian Journal of Dairy Technology 51, 811.
Institut National de la Recherche Agronomique 1989. Ruminant nutrition, recommended allowances and feed tables (ed. R Jarrige). INRA, Paris and John Libbey Eurotext, London.
Keane, CJ, Hanlon, AJ, Roche, JF, Burton, JL, Mee, JF, O’Doherty, JV, Sweeney, T 2006. Short communication: a potential antiapoptotic phenotype in neutrophils of cows milked once daily in early lactation. Journal of Dairy Science 89, 10241027.
Kelly, AL, Reid, S, Joyce, P, Meaney, W, Foley, J 1998. Effect of decreased milking frequency of cows in late lactation on milk somatic cell count, polymorphonuclear leukocyte numbers, composition and proteolytic activity. Journal of Dairy Research 65, 365373.
Klei, LR, Lynch, JM, Barbano, DM, Oltenacu, PA, Lednor, AJ, Bandler, DK 1997. Influence of milking three times a day on milk quality. Journal of Dairy Science 80, 427436.
Knutson, RJ, Mackenzie, DDS, Davis, SR, McCutcheon, SN 1993. The effect of once daily milking on concentrations and yields of plasminogen, plasmin and other whey proteins. Proceedings of the New Zealand Society of Animal Production 53, 155158.
Lacy-Hulbert, SJ, Woolford, MW, Nicholas, GD, Prosser, CG, Stelwagen, K 1999. Effect of milking frequency and pasture intake on milk yield and composition of late lactation cows. Journal of Dairy Science 82, 12321239.
Macheboeuf, D, Coulon, JB, D’hour, P 1993. Effect of breed, protein genetic variants and feeding on cows’ milk coagulation properties. Journal of Dairy Research 60, 4354.
Martin, B, Coulon, JB 1995. Milk production and cheese characteristics. I. Influence of milk production conditions on coagulability of bulk milk. Lait 75, 6180.
Martin B, Pradel P and Verdier-Mezt I 2000. Effet de la race (Holstein/Montbéliarde) sur les caractéristiques chimiques et sensorielles des fromages. Proceedings of 7e Rencontres autour des Recherches sur les Ruminants, Paris, France, p. 317.
McMahon, DJ, Brown, RJ 1982. Evaluation of formagraph for comparing rennet solutions. Journal of Dairy Science 65, 16391642.
O’Brien, B, Ryan, G, Meaney, WJ, McDonagh, D, Kelly, A 2002. Effect of frequency of milking on yield, composition and processing quality of milk. Journal of Dairy Research 69, 367374.
Pomiès D, Rémond B and Pradel P 2004. Performances des vaches laitières et qualité du lait lors de la monotraite et après retour à 2 traites par jour, en fonction de la durée de cette pratique et du stade de lactation des animaux. Proceedings of 11e Rencontres autour des Recherches sur les Ruminants, Paris, France, pp. 225–228.
Prosser, CG, Nicholas, G, Stelwagen, K, Lacy-Hulbert, SJ, Woolford, MW, McFadden, TB 1995. Influence of milking frequency and feeding level on plasmin activity and casein degradation in milk. Proceedings of the New Zealand Society of Animal Production 55, 911.
Rémond, B, Coulon, JB, Nicloux, M, Levieux, D 1999. Effect of temporary once-daily milking in early lactation on milk production and nutritional status of dairy cows. Annales de Zootechnie 48, 341352.
Rémond, B, Aubailly, S, Chilliard, Y, Dupont, D, Pomiès, D, Petit, M 2002. Combined effects of once-daily milking and feeding level in the first three weeks of lactation on milk production and enzyme activities, and nutritional status, in Holstein cows. Animal Research 51, 101117.
Rémond B, Pomiès D and Brunschwig G 2003. La traite une fois par jour de vaches Prim’Holstein, pendant une semaine, diminue la production laitière de 25%, sans effet rémanent. Proceedings of 10e Rencontres autour des Recherches sur les Ruminants, Paris, France, p. 113.
Rémond, B, Pomiès, D, Dupont, D, Chilliard, Y 2004. Once-a-day milking of multiparous Holstein cows throughout the entire lactation: milk yield and composition, and nutritional status. Animal Research 53, 201212.
Rémond, B, Pomiès, D 2005. Once-daily milking of dairy cows: a review of recent French experiments. Animal Research 54, 427442.
Rowland, SJ 1938. The determination of the nitrogen distribution in milk. Journal of Dairy Research 9, 4246.
Statistical Analysis Systems Institute 1999. SAS/STAT user’s guide, version 8. SAS Institute Inc., Cary, NC, USA.
Stelwagen, K, Davis, SR, Farr, VC, Prosser, CG, Sherlock, RA 1994. Mammary epithelial cell tight junction integrity and mammary blood flow during an extended milking interval in goats. Journal of Dairy Science 77, 426432.
Stelwagen, K, Lacy-Hulbert, SJ 1996. Effect of milking frequency on milk somatic cell count characteristics and mammary secretory cell damage in cows. American Journal of Veterinary Research 57, 902905.
Stelwagen, K, Espen, DCV, Verkerk, GA, McFadden, HA, Farr, VC 1998a. Elevated plasma cortisol reduces permeability of mammary tight junctions in the lactating bovine mammary epithelium. Journal of Endocrinology 159, 173178.
Stelwagen, K, Mclaren, RD, Turner, SA, Mcfadden, HA, Prosser, CG 1998b. No evidence for basolateral secretion of milk protein in the mammary gland of lactating goats. Journal of Dairy Science 81, 434437.
Tucker, CB, Dalley, DE, Burke, JL, Clark, DA 2007. Milking cows once daily influences behavior and udder firmness at peak and mid lactation. Journal of Dairy Science 90, 16921703.
Verdier-Metz, I, Coulon, JB, Pradel, P, Viallon, C, Albouy, H, Berdagué, JL 2000. Effect of the botanical composition of hay and casein genetic variants on the chemical and sensory characteristics of ripened Saint-Nectaire type cheeses. Lait 80, 361370.


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Once-a-day milking of Holstein and Montbéliarde cows for 7 weeks in mid-lactation

  • D. Pomiès (a1), B. Martin (a1), Y. Chilliard (a1), P. Pradel (a2) and B. Rémond (a3)...


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