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MicroRNA-27a promotes porcine myoblast proliferation by downregulating myostatin expression

  • T. Yang (a1), X. L. Chen (a1), Z. Q. Huang (a1), W. X. Wen (a1), M. Xu (a1), D. W. Chen (a1), B. Yu (a1), J. He (a1), J. Q. Luo (a1), J. Yu (a1), X. B. Mao (a1) and P. Zheng (a1)...

Abstract

MicroRNAs are endogenous ~22nt RNAs that negatively regulate gene expression at the posttranscriptional level via binding to the 3′-untranslated region (3′UTR) of target mRNAs. The microRNA miR-27a was reported to depress the expression of myostatin, a critical inhibitor of skeletal myogenesis, by binding to its 3′UTR in mouse. In this study, we cloned the full-length 3′UTR of porcine myostatin by rapid amplification of 3′-cDNA ends (3′-RACE) and demonstrated that the 3′UTR of porcine myostatin is targeted by miR-27a. The phenomenon that the level of myostatin inversely correlated with miR-27a was observed in fat and heart of pigs and also in proliferating porcine myoblasts. Besides, overexpression of miR-27a in porcine myoblasts promoted cell proliferation by reducing the expression of myostatin. Our data suggest that miR-27a positive regulates porcine myoblast proliferation via targeting myostatin.

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Corresponding author

E-mail: zqhuang@sicau.edu.cn

References

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animal
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