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Leucine alters immunoglobulin a secretion and inflammatory cytokine expression induced by lipopolysaccharide via the nuclear factor-κB pathway in intestine of chicken embryos

Published online by Cambridge University Press:  22 December 2017

S. Q. Liu ,
L. Y. Wang ,
G. H. Liu ,
D. Z. Tang ,
X. X. Fan ,
J. P. Zhao ,
H. C. Jiao ,
X. J. Wang ,
S. H. Sun  and
H. Lin
S. Q. Liu
Affiliation:
Department of Animal Science, Shandong Agricultural University, Daizong Street No.61, Taian, Shandong 271018, China School of Basic Medical Sciences, Taishan Medical College, Yingsheng East Road No.2, Taian 271016, China
L. Y. Wang
Affiliation:
Department of Animal Science, Shandong Agricultural University, Daizong Street No.61, Taian, Shandong 271018, China
G. H. Liu
Affiliation:
School of Basic Medical Sciences, Taishan Medical College, Yingsheng East Road No.2, Taian 271016, China
D. Z. Tang
Affiliation:
Department of Animal Science, Shandong Agricultural University, Daizong Street No.61, Taian, Shandong 271018, China
X. X. Fan
Affiliation:
Department of Animal Science, Shandong Agricultural University, Daizong Street No.61, Taian, Shandong 271018, China
J. P. Zhao
Affiliation:
Department of Animal Science, Shandong Agricultural University, Daizong Street No.61, Taian, Shandong 271018, China
H. C. Jiao
Affiliation:
Department of Animal Science, Shandong Agricultural University, Daizong Street No.61, Taian, Shandong 271018, China
X. J. Wang
Affiliation:
Department of Animal Science, Shandong Agricultural University, Daizong Street No.61, Taian, Shandong 271018, China
S. H. Sun*
Affiliation:
Department of Animal Science, Shandong Agricultural University, Daizong Street No.61, Taian, Shandong 271018, China
H. Lin*
Affiliation:
Department of Animal Science, Shandong Agricultural University, Daizong Street No.61, Taian, Shandong 271018, China
*
†E-mail: sunshuhong@sdau.edu.cn; hailin@sdau.edu.cn
†E-mail: sunshuhong@sdau.edu.cn; hailin@sdau.edu.cn
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Abstract

The mammalian target of rapamycin (mTOR) has been shown to be involved in lipopolysaccharide (LPS)-induced immune responses in many mammal cells. Here, we suggest that the mTOR pathway is involved in the intestinal inflammatory responses evoked by LPS treatment in chicken embryos. The intestinal tissue from Specific pathogen free chick embryos was cultured in the presence of LPS for 2 h. Secretory immunoglobulin A (sIgA) concentrations, messenger RNA (mRNA) expression of cytokines, and protein levels of nuclear factor-κB (NF-κB), mitogen-activated protein kinase (MAPK), mTOR and p70 ribosomal S6 kinase (p70S6K) were determined. The results showed that LPS treatment increased sIgA concentrations in a dose-dependent manner. The mRNA levels of interleukine (IL)-6, IL-8, IL-10, tumor necrosis factor-α and Toll-like receptor (TLR) 4 were upregulated by LPS treatment (P<0.05). Lipopolysaccharide increased the phosphorylation of Jun N-terminal kinase (JNK), p38 MAPK and NF-κB (P<0.05) while decreasing the phosphorylation level of mTOR (P<0.05). Supplementation of leucine at doses of 10, 20 and 40 mM dose-dependently decreased sIgA production. Leucine supplementation at 40 mM restored the phosphorylation level of mTOR and p70S6K while suppressing the phosphorylation levels of NF-κB (P<0.05) and partially down-regulating the phosphorylation of p38 MAPK and JNK. The transcription of IL-6 was significantly decreased by leucine supplementation. These results suggested that leucine could alleviate LPS-induced inflammatory responses by down-regulating NF-κB signaling pathway and evoking mTOR/p70S6K signaling pathway, which may involve in the regulation of the intestinal immune system in chicken embryos.

Keywords

inflammatory cytokinesecretory immunoglobulin Anuclear factor-κBleucinemammalian target of rapamycin/p70 ribosomal S6 kinase
Type
Research Article
Information
animal , Volume 12 , Issue 9 , September 2018 , pp. 1903 - 1911
Copyright
© The Animal Consortium 2017 

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