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A genome scan for loci affecting adipocyte size and number in abdominal fat in a White Duroc × Erhualian F2 resource population

Published online by Cambridge University Press:  01 May 2009

L. T. Liu ,
J. Ren ,
L. Li ,
W. B. Li ,
B. Yang ,
W. C. Zhu ,
B. L. Guo ,
J. W. Ma  and
L. S. Huang
L. T. Liu
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045 Nanchang, China
J. Ren
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045 Nanchang, China
L. Li
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045 Nanchang, China
W. B. Li
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045 Nanchang, China
B. Yang
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045 Nanchang, China
W. C. Zhu
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045 Nanchang, China
B. L. Guo
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045 Nanchang, China
J. W. Ma
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045 Nanchang, China
L. S. Huang*
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045 Nanchang, China
*
E-mail: Lushenghuang@hotmail.com
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Abstract

Adipocyte size and number are correlated with fat deposition, which is of major concern to human health and pork producers. To identify quantitative trait loci (QTL) for adipocyte size and number in pigs, a total of 341 F2 animals at 240 days in a White Duroc × Erhualian cross were measured for the area, perimeters, volume and number of adipocyte in abdominal fat. A genome scan was performed on these animals and their parents and grandparents with 183 microsatellite markers spanning the pig genome. Five chromosomal regions showed effects on the traits measured, predominantly on adipocyte size, on pig chromosome (SSC) 1, 4, 7 and 9. Neither of these QTL has been reported before this study. The QTL for adipocyte size detected in this study perfectly correspond to the previously reported QTL for fatness traits on SSC1, 4 and 7. The most significant association was evidenced at 58 cM on SSC7. At the locus, the favorable allele decreasing adipocyte size was unusually originated from the obese Erhualian breed. Only a suggestive QTL was detected for adipocyte number on SSC9. The results shed new lights on the understanding of the genetic basis of fatness traits in pigs.

Keywords

adipocyte numberadipocyte sizepigQTL
Type
Full Paper
Information
animal , Volume 3 , Issue 5 , May 2009 , pp. 632 - 636
Copyright
Copyright © The Animal Consortium 2009

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References

Anderson, DB, Kauffman, RG 1972. Cellular and enzymatic changes in porcine adipose tissue during growth. Journal of Lipid Research 14, 160–168.CrossRefGoogle Scholar
Bidanel, JP, Milan, D, Iannuccelli, N, Amigues, Y, Boscher, MY, Bourgeois, F, Caritez, JC, Gruand, J, Le Roy, P, Lagant, H, Quintanilla, R, Renard, C, Gellin, J, Ollivier, L, Chevalet, C 2001. Detection of quantitative trait loci for growth and fatness in pigs. Genetic Selection Evolution 33, 289–309.CrossRefGoogle ScholarPubMed
Cepica, S, Stratil, A, Kopecny, M, Blazkova, P, Schröffel, J Jr, Davoli, R, Fontanesi, L, Reiner, G, Bartenschlager, H, Moser, G, Geldermann, H 2003. Linkage and QTL mapping for Sus scrofa chromosome 4. Journal of Animal Breeding and Genetics 120, 28–37.CrossRefGoogle Scholar
Cepica, S, Bartenschlager, H, Geldermann, H 2007. Mapping of QTL on chromosome X for fat deposition, muscling and growth traits in a wild boar × Meishan F2 family using a high-density gene map. Animal Genetics 36, 634–638.CrossRefGoogle Scholar
Churchill, GA, Doerge, RW 1994. Empirical threshold values for quantitative trait mapping. Genetics 138, 963–971.CrossRefGoogle ScholarPubMed
de Koning, DJ, Janss, LL, Rattink, AP, van Oers, PA, de Vries, BJ, Groenen, MA, van der Poel, JJ, de Groot, PN, Brascamp, EW, van Arendonk, JA 1999. Detection of quantitative trait loci for backfat thickness and intramuscular fat content in pigs (Sus scrofa). Genetics 152, 1679–1690.CrossRefGoogle ScholarPubMed
Haley, CS, Knott, SA, Elsen, JM 1994. Mapping quantitative trait loci in crosses between outbred lines using least squares. Genetics 136, 1195–1207.CrossRefGoogle ScholarPubMed
Hood, RL, Allen, CE 1977. Cellularity of porcine adipose tissue: effects of growth and adiposity. Journal of Lipid Research 18, 275–284.CrossRefGoogle ScholarPubMed
Knott, SA, Marklund, L, Haley, CS, Andersson, K, Davies, W, Ellegren, H, Fredholm, M, Hansson, I, Hoyheim, B, Lundström, K, Moller, M, Andersson, L 1998. Multiple marker mapping of quantitative trait loci in a cross between outbred wild boar and large white pigs. Genetics 149, 1069–1080.CrossRefGoogle Scholar
Lau, DC 2004. Adipose tissue growth and differentiation: view from the chair. International Journal of Obesity and Related Metabolic Disorders 24, S20–S22.CrossRefGoogle Scholar
Liu, G, Jennen, DG, Tholen, E, Juengst, H, Kleinwächter, T, Hölker, M, Tesfaye, D, Un, G, Schreinemachers, HJ, Murani, E, Ponsuksili, S, Kim, JJ, Schellander, K, Wimmers, K 2007. A genome scan reveals QTL for growth, fatness, leanness and meat quality in a Duroc–Pietrain resource population. Animal Genetics 38, 241–252.CrossRefGoogle Scholar
Marklund, L, Nyström, PE, Stern, S, Andersson-Eklund, L, Andersson, L 1999. Confirmed quantitative trait loci for fatness and growth on pig chromosome 4. Heredity 82, 134–141.CrossRefGoogle ScholarPubMed
Milan, D, Bidanel, JP, Iannuccelli, N, Riquet, J, Amigues, Y, Gruand, J, Le Roy, P, Renard, C, Chevalet, C 2002. Detection of quantitative trait loci for carcass composition traits in pigs. Genetic Selection Evolution 34, 705–728.CrossRefGoogle ScholarPubMed
Nagamine, Y, Haley, CS, Sewalem, A, Visscher, PM 2003. Quantitative trait loci variation for growth and obesity between and within lines of pigs (Sus scrofa). Genetics 164, 629–635.CrossRefGoogle ScholarPubMed
Norman, RA, Bogardus, C, Ravussin, E 1995. Linkage between obesity and a marker near the tumor necrosis factor-α locus in Pima Indians. Journal of Clinical Investigation 96, 158–162.CrossRefGoogle Scholar
Ren, DR, Ren, J, Xing, YY, Guo, YM, Wu, YB, Yang, GC, Mao, HR, Huang, LS 2008. A genome scan for quantitative trait loci affecting male reproductive traits in a White Duroc × Chinese Erhualian resource population. Journal of Animal Science 86 (available online July 3, 2008 as doi:10.2527/jas.2008-0923).Google Scholar
Rohrer, GA, Keele, JW 1998. Identification of quantitative trait loci affecting carcass composition in swine: I. Fat deposition traits. Journal of Animal Science 76, 2247–2254.CrossRefGoogle ScholarPubMed
Rothschild, MF, Hu, ZL, Jiang, Z 2007. Advances in QTL mapping in pigs. International Journal of Biological Sciences 3, 192–197.CrossRefGoogle ScholarPubMed
Sanchez, MP, Riquet, J, Iannuccelli, N, Gogué, J, Billon, Y, Demeure, O, Caritez, JC, Burgaud, G, Fève, K, Bonnet, M, Péry, C, Lagant, H, Le Roy, P, Bidanel, JP, Milan, D 2006. Effects of quantitative trait loci on chromosome 1, 2, 4, and 7 on growth, carcass and meat quality traits in backcross Meishan × Large White pigs. Journal Animal of Science 84, 526–537.CrossRefGoogle ScholarPubMed
Sato, S, Oyamada, Y, Atsuji, K, Nade, T, Sato, S, Kobayashi, E, Mitsuhashi, T, Nirasawa, K, Komatsuda, A, Saito, Y, Terai, S, Hayashi, T, Sugimoto, Y 2003. Quantitative trait loci for growth and carcass traits in a Meishan × Duroc F2 resource population. Journal of Animal Science 81, 2938–3949.CrossRefGoogle Scholar
Sjöström, L, Björntorp, P, Vrána, J 1971. Microscopic fat cell size measurements on frozen-cut adipose tissue in comparison with automatic determinations of osmium-fixed fat cells. Journal of Lipid Research 12, 521–530.CrossRefGoogle ScholarPubMed
van Laere, AS, Nguyen, M, Braunschweig, M, Nezer, C, Collette, C, Moreau, L, Archibald, AL, Haley, CS, Buys, N, Tally, M, Andersson, G, Georges, M, Andersson, L 2003. A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig. Nature 425, 832–836.CrossRefGoogle Scholar
Varona, L, Ovilo, C, Clop, A, Noguera, JL, Pérez-Enciso, M, Coll, A, Folch, JM, Barragán, C, Toro, MA, Babot, D, Sánchez, A 2002. QTL mapping for growth and carcass traits in an Iberian by Landrace pig intercross: additive, dominant and epistatic effects. Genetical Research 80, 145–154.CrossRefGoogle Scholar
Visscher, PM, Thompson, R, Haley, CS 1996. Confidence interval s in QTL mapping by bootstrapping. Genetics 143, 1013–1020.CrossRefGoogle ScholarPubMed
Walling, GA, Archibald, AL, Cattermole, JA, Downing, AC, Finlayson, HA, Nicholson, D, Visscher, PM, Walker, CA, Haley, CS 1998. Mapping of quantitative trait loci on porcine chromosome 4. Animal Genetics 29, 415–424.CrossRefGoogle ScholarPubMed
Walling, GA, Visscher, PM, Andersson, L, Rothschild, MF, Wang, L, Moser, G, Groenen, MA, Bidanel, JP, Cepica, S, Archibald, AL, Geldermann, H, de Koning, DJ, Milan, D, Haley, CS 2000. Combined analyses of data from quantitative trait loci mapping studies. Chromosome 4 effects on porcine growth and fatness. Genetics 155, 1369–1378.CrossRefGoogle ScholarPubMed
Wang, L, Yu, TP, Tuggle, CK, Liu, HC, Rothschild, MF 1998. A directed search for quantitative trait loci on chromosome 4 and 7 in pigs. Journal of Animal Science 76, 2560–2567.CrossRefGoogle ScholarPubMed
Zhang, ZG, Li, BD, Chen, XH 1986. Pig Breeds in China. Shanghai Scientific and Technical Publisher, Shanghai.Google Scholar