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Genetic analysis of haematological and plasma biochemical parameters in the Spanish purebred horse exercised on a treadmill

  • B. M. Escribano (a1), A. Molina (a2), M. Valera (a3), P. Tovar (a1), E. I. Agüera (a1), R. Santisteban (a1), R. Vivo (a1), S. Agüera (a1) and M. D. Rubio (a1)...


The novel aim of this study was to describe the reference values of different haematological and biochemical parameters in the Spanish purebred horse (Andalusian, SPB) in each of the stages of a programmed exercise on a treadmill system, and to establish heritability and genetic correlations for these haematological and biochemical parameters. For this, 94 young SPB male horses (4.22 ± 2.27 years old) were used. An increasing intensity exercise test at 4, 5, 6 and 7 m/s was carried out on a treadmill (6% inclination). Total red blood cells, total white blood cells, neutrophils and lymphocytes counts; haematocrit, haemoglobin, lactate, uric acid, creatinine and total plasma proteins concentrations and aspartate transaminase, lactate dehydrogenase, creatine-quinase activities were determined. To conclude: (i) the reference values for each parameter were determined for each of the exercise test stages (ii) all the parameters analysed manifested a medium-high heritability and a high repeatability. These results will, in the near future, determine the measuring guidelines for improving the SPB horse's athletic ability on an objective treadmill system and for selecting these animals in response to those parameters.


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American Society for Veterinary Clinical Pathology (ASVCP) 2005. 40th Annual Meeting: In Conjunction with the American College of Veterinary Pathologists (ACVP) 56th Annual Meeting, Boston, Massachusetts, December 3–7, 2005.
Barrey, E 2010. Genetics and genomics in equine exercise physiology: an overview of the new applications of molecular biology as positive and negative markers of performance and health. Equine Veterinary Journal 42, 561568.
Barrey, E, Courouce, A, Langlois, B, Blouin, CAuvinet, B 1999. Genetic component of exercise test parameters in French Trotters: first estimations. In Conference on equine sports medicine and science (ed. A Lindner), pp. 219-224. Wageningen Press, Wageningen, Cordoba.
Bis-Wencel, H, Lutnicki, K, Rowicka, AZ, Nowakowicz-Dębek, B, Bryl, M 2012. Effort of varying intensity as a factor influencing the variability of selected biochemical blood parameters of jumping horses. Bulletin of the Veterinary Institute in Pulawy 56, 225229.
Cano, MR, Miró, F, Vivo, J, Galisteo, AM 2000. Influence of training on the biokinematic study of young and adult Andalusian horses at the trot. Journal of Veterinary Medicine Series A 46, 91101.
Evans, DL 2007. Physiology of equine performance and associated tests of function. Equine Veterinary Journal 39, 373383.
Escribano, BM, Castejón, FM, Santisteban, R, Agüera, EI, Rubio, MD 1995. Effect of training on diverse hematologic parameters in Andalusian horses. Revista Española de Fisiología 51, 207212.
Escribano, BM, Agüera, EI, Rubio, MD, Santisteban, R, Vivo, R, Agüera, EI, Tovar, P 2011. Utility of the discriminant analysis to categorize untrained Spanish pure bred horses on the basis of biochemical and muscle variables before and after a standardized exercise test. Research in Veterinary Science 91, 144148.
Fraipont, A, Van Erck, E, Ramery, E, Fortier, G, Lekeux, P, Art, T 2012. Assessing fitness in endurance horses. Canadian Veterinary Journal 53, 311314.
Giulotto, E 2001. Will horse genetics create better champions? Trends in Genetics 17, 166.
Groeneveld, E 1996. REML VCE a multivariate multil model restricted maximum likelihood (Co) variance component estimation package, versión 3.2, User's guide. In (ed. E Groeneveld), Institute of animal husbandry and animal ethology. Federal Research Center of Agriculture, Neustadt, Germany.
Groeneveld, E, Kovac, M, Mielenz, N 2010. VCE user's guide and reference manual. Version 6.0.2 Institute of Farm Animal Genetics, Neustadt, Germany.
Gurgoze, SY, Icen, H 2010. The influence of age on clinical biochemical parameters in pure-bred Arabian mares. Journal of Equine Veterinary Science 30, 569574.
International Federation of Clinical Chemistry, Scientific Committee, Clinical Section Expert Panel on the Theory of Reference Values 1987. Provisional recommendation on the theory of reference values. Part 5: statistical treatment of selected reference values. Determination of reference limits. Clinica Chimica Acta 170, S13S32.
Knottenbelt, D 2006. Vital signs, normal values. In Saunders equine formulary (ed WB Saunders), pp. 7. Co Saunders Elsevier, Philadelphia, USA.
López-Rivero, JL, Agüera, E, Monterde, JG, Rodríguez, MV, Miró, F 1989. Comparative study of muscle fiber type composition in the middle gluteal muscle of Andalusian, Thoroughbred and Arabian horses. Journal of Equine Veterinary Science 9, 337340.
Molina, A, Valera, M, Dos Santos, R, Rodero, A 1999. Genetic parameters of morphofunctional traits in Andalusian horse. Livestock Production Science 60, 295303.
Molina, A, Valera, M, Galisteo, AM, Vivo, J, Gómez, MD, Rodero, A, Agüera, E 2008. Genetic parameters of biokinematic variables at walk in the Spanish purebred (Andalusian) horse using experimental treadmill records. Livestock Science 116, 137145.
Muñoz, A, Riber, C, Trigo, P, Castejón, F 2012. Age-and gender-related variations in hematology, clinical biochemistry, and hormones in Spanish fillies and colts. Research in Veterinary Science 93, 943949.
Muñoz, A, Riber, C, Santisteban, R, Lucas, R, Castejón, FM 2002. Effect of training duration and exercise on blood-borne substrate, plasma lactate and enzyme concentrations in Andalusian, Anglo-Arabian, and Arabian breeds. Equine Veterinary Journal Supplement 34, 245251.
Pritchard, JC, Burn, CC, Barr, ARS, Whay, HR 2009. Haematological and serum biochemical reference values for apparently healthy working horses in Pakistan. Research in Veterinary Science 87, 389395.
Ricard, A, Legarra, A 2010. Validation of models for analysis of ranks in horse breeding evaluation. Genetics Selection Evolution 42, 110.
Rose, RJ, Hodgson, DR 1994. Clinical and exercise testing. In The athletic horse (ed. DR Hodgson and RJ Rose), pp. 246257. W.B. Saunders Company, Philadelphia, USA.
Rubio, MD, Muñoz, A, Santisteban, R, Tovar, P, Castejón, FM 1995. Comparative hematological study of two breeds of foals (Andalusian and Arab) subjected to exercise of progressive intensity. Journal of Veterinary Medicine Science 57, 311315.
Rubio, MD, Escribano, BM, Oropesa, A, Tovar, P, Castejón, FM, Vivo, R 1996. Inluence of trotting and galloping exercises on erythrogram of Andalusian horse stallions. Journal of Equine Veterinary Science 16, 249253.
Sánchez, MJ, Valera, M, Gómez, MD, Cervantes, I, Gutiérrez, JP 2012. Modelling the rider effect in the genetic evaluation of dressage in Spanish purebred horses. Book of Abstracts of the 63th Annual Meeting of the European Association for Animal Production. Ed. Elservier, 34pp.
Satue, K, Blanco, O, Muñoz, A 2009. Age-related differences in the hematological profile of Andalusian broodmares of Carthusian strain. Veterinarni Medicina 54, 175182.
Schuback, K, Essén-Gustavsson, B 1998. Muscle anaerobic response to a maximal treadmill exercise test in Standardbreed trotters. Equine Veterinary Journal 30, 504510.
Simenew, K, Gezahegne, M, Getachew, M, Wondyefraw, M, Alemayehu, L, Eyob, I 2011. Reference values of clinically important physiological, haematological and serum biochemical parameters of apparently healthy working equids of Ethiopia. Global Veterinaria 7, 16.
Tolley, EA, Notter, DR, Marlowe, DJ 1983. Heritability and repeteability of speed for 2-and-3-year- old Standarbred racehorses. Journal of Animal Science 56, 12941305.
Valera, M, Gómez, MD, Cervantes, Iand Molina, A 2006. Genetic Evaluation of dressage performance in Spanish Purebred Horses. Book of Abstracts of the 57th Annual Meeting of the European Association for Animal Production. Ed. Elservier, 352pp.
Valera, M, Gómez, MD, Molina, A, Rodero, A 2007. La valoración de la capacidad locomotora del caballo PRE en la selección de reproductores. In Foro de Opinión del caballo Español, 86124.
Valera, M, Molina, A, Gutiérrez, JP, Gómez, J, Goyache, F 2005. Pedigree analysis in the Andalusian horse: population structure, genetic variability and influence of the Carthusian strain. Livestock Production Science 95, 5766.
Valera, M, Galisteo, AM, Molina, A, Miró, F, Gómez, MD, Cano, MR, Agüera, E 2008. Genetic parameters of biokinematic variables of the trot in Spanish Purebred horses under experimental treadmill conditions. The Veterinary Journal 178, 217224.


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Genetic analysis of haematological and plasma biochemical parameters in the Spanish purebred horse exercised on a treadmill

  • B. M. Escribano (a1), A. Molina (a2), M. Valera (a3), P. Tovar (a1), E. I. Agüera (a1), R. Santisteban (a1), R. Vivo (a1), S. Agüera (a1) and M. D. Rubio (a1)...


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