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Erythropoietin non-viral gene therapy does not affect motility, viability, morphology or concentration of rabbit sperm

  • T. F. Collares (a1), V. F. Campos (a1), G. Urtiaga (a1), P. M. M. Leon (a1), M. G. Amaral (a1), C. P. Hartleben (a1), A. J. McBride (a1), O. A. Dellagostin (a1), J. C. Deschamps (a1), F. K. Seixas (a1) and T. Collares (a1)...


Erythropoietin (EPO) gene therapy can be used for several purposes; however, its effects on reproductive performance are unknown. The aim of this study was to evaluate the toxicological effects of non-viral (EPO) gene transfer on sperm motility, viability, morphology and concentration. Rabbit EPO cDNA was cloned into a pTarget mammalian expression vector. Rabbits were administered with: (1) pTarget/EPO vector, (2) recombinant human EPO (rHuEpo) and (3) saline (control). Both pTarget/EPO and rHuEpo significantly increased (P < 0.05) hematocrit levels 1 week after injection and they remained significantly higher than the control for up to 5 weeks (P < 0.05), showing that both EPO treatments were effective in stimulating the production of red blood cells in rabbits. The EPO gene transfer or rHuEPO administration had no significant effect (P > 0.05) on sperm motility, vigor, viability, concentration or morphology in the testis.


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Azzazy, HM, Mansour, MM, Christenson, RH 2009. Gene doping: of mice and men. Clinical Biochemistry 42, 435441.
Campos, VF, de Leon, PM, Komninou, ER, Dellagostin, OA, Deschamps, JC, Seixas, FK, Collares, T 2011a. NanoSMGT: Transgene transmission into bovine embryos using halloysite clay nanotubes or nanopolymer to improve transfection efficiency. Theriogenology 76, 15521560.
Campos, VF, Komninou, ER, Urtiaga, G, de Leon, PM, Seixas, FK, Dellagostin, OA, Deschamps, JC, Collares, T 2011b. NanoSMGT: Transfection of exogenous DNA on sex-sorted bovine sperm using nanopolymer. Theriogenology 75, 14761481.
Foresta, C, Mioni, R, Bordon, P, Miotto, D, Montini, G, Varotto, A 1994. Erythropoietin stimulates testosterone production in man. Journal of Clinical Endocrinology and Metabolism 78, 753756.
Friedmann, T 2010. How close are we to gene doping? Hastings Center Reports 40, 2022.
Friedmann, T, Rabin, O, Frankel, MS 2010. Ethics. Gene doping and sport. Science 327, 647648.
Haisma, HJ, de, HO 2006. Gene doping. International Journal of Sports Medicine 27, 257266.
Jelkmann, W 2003. Erythropoietin. Journal of Endocrinological Investigation 26, 832837.
Kobayashi, T, Yanase, H, Iwanaga, T, Sasaki, R, Nagao, M 2002. Epididymis is a novel site of erythropoietin production in mouse reproductive organs. Biochemical and Biophysical Research Communications 296, 145151.
Köseoğlu, B, Yilmaz, E, Ceylan, K, Uzun, E, Bayram, I, Hizli, F 2009. The protective effect of erythropoietin infusion on testicular torsion/detorsion: an experimental study. International Urology and Nephrology 41, 8591.
Lacombe, C, Mayeux, P 1998. Biology of erythropoietin. Haematologica 83, 724732.
Marklund, S, Sandberg, K, Andersson, L 1996. Forensic tracing of horse identities using urine samples and DNA markers. Animal Biotechnology 7, 145153.
Maruyama, H, Ataka, K, Gejyo, F, Higuchi, N, Ito, Y, Hirahara, H, Imazeki, I, Hirata, M, Ichikawa, F, Neichi, T, Kikuchi, H, Sugawa, M, Miyazaki, J 2001. Long-term production of erythropoietin after electroporation-mediated transfer of plasmid DNA into the muscles of normal and uremic rats. Gene Therapy 8, 461468.
McKanna, TA, Toriello, HV 2010. Gene doping: the hype and the harm. Pediatric Clinics of North America 57, 719727.
Sharp, NC 2010. The human genome and sport, including epigenetics, gene doping, and athleticogenomics. Endocrinology Metabolism Clinics of North America 39, 201215, xi.
Temma, K, Shimoya, K, Hashimoto, K, Zhang, Q, Koyama, M, and Murata, Y 2004. Detection of erythropoietin in human seminal plasma. Fertility and Sterility 81 (Suppl 1), 798801.
Tug, N, Altunkaynak, ME, Aktas, RG, Kilic, U, Yilmaz, B, Cam, C, Karateke, A 2010. Does erythropoietin affect motility of spermatozoa? Archives of Gynecology and Obstetrics 281, 933938.
Wells, DJ 2008. Gene doping: the hype and the reality. British Journal of Pharmacology 154, 623631.
Wells, DJ 2009. Gene doping: possibilities and practicalities. Medicine and Sport Science 54, 166175.
Yamamoto, Y, Sofikitis, N, and Miyagawa, I 1997. Effects of erythropoietin, bromocryptine and hydralazine on testicular function in rats with chronic renal failure. Andrologia 29, 141144.
Yamazaki, T, Kanzaki, M, Kamidono, S, Fujisawa, M 2004. Effect of erythropoietin on Leydig cell is associated with the activation of Stat5 pathway. Molecular and Cellular Endocrinology 213, 193198.
Yasuda, Y, Fujita, Y, Musha, T, Tanaka, H, Shiokawa, S, Nakamatsu, K, Mori, S, Matsuo, T, Nakamura, Y 2001. Expression of erythropoietin in human female reproductive organs. Archivio Italiano di Anatomia e di Embriologia 106, 215222.


Erythropoietin non-viral gene therapy does not affect motility, viability, morphology or concentration of rabbit sperm

  • T. F. Collares (a1), V. F. Campos (a1), G. Urtiaga (a1), P. M. M. Leon (a1), M. G. Amaral (a1), C. P. Hartleben (a1), A. J. McBride (a1), O. A. Dellagostin (a1), J. C. Deschamps (a1), F. K. Seixas (a1) and T. Collares (a1)...


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