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Erythropoietin non-viral gene therapy does not affect motility, viability, morphology or concentration of rabbit sperm

Published online by Cambridge University Press:  22 November 2012

T. F. Collares ,
V. F. Campos ,
G. Urtiaga ,
P. M. M. Leon ,
M. G. Amaral ,
C. P. Hartleben ,
A. J. McBride ,
O. A. Dellagostin ,
J. C. Deschamps  and
F. K. Seixas
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T. F. Collares
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
V. F. Campos
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
G. Urtiaga
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
P. M. M. Leon
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
M. G. Amaral
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
C. P. Hartleben
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
A. J. McBride
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
O. A. Dellagostin
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
J. C. Deschamps
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
F. K. Seixas
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
T. Collares*
Affiliation:
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Campus Universitário s/n°, Caixa Postal 354, CEP 96010-900, Pelotas, Rio Grande do Sul, Brazil
*
†E-mail: collares.t@gmail.com
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Abstract

Erythropoietin (EPO) gene therapy can be used for several purposes; however, its effects on reproductive performance are unknown. The aim of this study was to evaluate the toxicological effects of non-viral (EPO) gene transfer on sperm motility, viability, morphology and concentration. Rabbit EPO cDNA was cloned into a pTarget mammalian expression vector. Rabbits were administered with: (1) pTarget/EPO vector, (2) recombinant human EPO (rHuEpo) and (3) saline (control). Both pTarget/EPO and rHuEpo significantly increased (P < 0.05) hematocrit levels 1 week after injection and they remained significantly higher than the control for up to 5 weeks (P < 0.05), showing that both EPO treatments were effective in stimulating the production of red blood cells in rabbits. The EPO gene transfer or rHuEPO administration had no significant effect (P > 0.05) on sperm motility, vigor, viability, concentration or morphology in the testis.

Keywords

EPOplasmid DNAsperm characteristicsrHuEPO
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 5 , May 2013 , pp. 778 - 783
Copyright
Copyright © The Animal Consortium 2012

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Footnotes

*

These authors contributed equally for this study.

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