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Erythropoietin non-viral gene therapy does not affect motility, viability, morphology or concentration of rabbit sperm

  • T. F. Collares (a1), V. F. Campos (a1), G. Urtiaga (a1), P. M. M. Leon (a1), M. G. Amaral (a1), C. P. Hartleben (a1), A. J. McBride (a1), O. A. Dellagostin (a1), J. C. Deschamps (a1), F. K. Seixas (a1) and T. Collares (a1)...

Abstract

Erythropoietin (EPO) gene therapy can be used for several purposes; however, its effects on reproductive performance are unknown. The aim of this study was to evaluate the toxicological effects of non-viral (EPO) gene transfer on sperm motility, viability, morphology and concentration. Rabbit EPO cDNA was cloned into a pTarget mammalian expression vector. Rabbits were administered with: (1) pTarget/EPO vector, (2) recombinant human EPO (rHuEpo) and (3) saline (control). Both pTarget/EPO and rHuEpo significantly increased (P < 0.05) hematocrit levels 1 week after injection and they remained significantly higher than the control for up to 5 weeks (P < 0.05), showing that both EPO treatments were effective in stimulating the production of red blood cells in rabbits. The EPO gene transfer or rHuEPO administration had no significant effect (P > 0.05) on sperm motility, vigor, viability, concentration or morphology in the testis.

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These authors contributed equally for this study.

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Keywords

Erythropoietin non-viral gene therapy does not affect motility, viability, morphology or concentration of rabbit sperm

  • T. F. Collares (a1), V. F. Campos (a1), G. Urtiaga (a1), P. M. M. Leon (a1), M. G. Amaral (a1), C. P. Hartleben (a1), A. J. McBride (a1), O. A. Dellagostin (a1), J. C. Deschamps (a1), F. K. Seixas (a1) and T. Collares (a1)...

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