Skip to main content Accessibility help

Effect of whole cottonseed v. sunflower seed on the fatty acid profile of subcutaneous fat, longissimus dorsi and blood of Thai Native and Holstein bulls

  • W. Polviset (a1), J. T. Schonewille (a2), H. Everts (a2), C. Wachirapakorn (a1), C. Yuangklang (a3), E. Claeys (a4) and S. De Smet (a4)...


In a 2×2 factorial design, 12 Thai Native and 12 Holstein bulls were fed ad libitum a total mixed ration (20 : 80; roughage : concentrate) with whole cottonseed (WCS) or sunflower seed (SFS) as oilseed sources. The rations contained 7% crude fat and were fed for 90 days. Plasma was taken at three times during the experiment, and at slaughter the longissimus dorsi and subcutaneous fat were sampled for fatty acid analysis. Ration did not affect rumen fermentation parameters. The plasma fatty acid profile was not affected by ration. In subcutaneous fat, a ration×breed interaction for the saturated fatty acid (SFA) and c9t11 CLA proportions was observed, resulting from larger differences between the rations in Thai Native compared with Holstein bulls. The WCS ration resulted in higher proportions of SFA and lower proportions of monounsaturated fatty acids and c9,t11 CLA compared with the SFS ration (P<0.01). In the intramuscular fat, the WCS ration was also associated with a lower c9t11 CLA proportion (P<0.01) and higher SFA proportion (P<0.05). The intramuscular proportion of polyunsaturated acids was higher and the proportion of SFA was lower in Thai Native compared with Holstein bulls (P<0.05), irrespective of ration.


Corresponding author


Hide All
Archibeque, SL, Lunt, DK, Gilbert, CD, Tume, RK and Smith, SB 2005. Fatty acid indices of stearoyl-CoA desaturase do not reflect actual stearoyl-CoA desaturase enzyme activities in adipose tissues of beef steers finished with corn-, flaxseed-, or sorghum-based diets. Journal of Animal Science 83, 11531166.
Association of Official Analytical Chemists 2004. Official methods of analysis vol. 2, 18th edition. Association of Official Analytical Chemists, Arlington, VA, USA.
Beatty, DT, Barnes, A, Taylor, E, Pethick, D, McCarthy, M and Maloney, SK 2006. Physiological responses of Bos taurus and Bos indicus cattle to prolonged, continuous heat and humidity. Journal of Animal Science 84, 972985.
De La Torre, A, Gruffat, D, Durand, D, Micol, D, Peyron, A, Scislowski, V and Bauchart, D 2006. Factors influencing proportion and composition of CLA in beef. Meat Science 73, 258268.
De Smet, S, Raes, K and Demeyer, D 2004. Meat fatty acid composition as affected by fatness and genetic factors: a review. Animal Research 53, 8198.
Folch, J, Lees, M and Stanley, SGH 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497509.
Givens, DI, Kliem, KE and Gibbs, RA 2006. The role of meat as a source of n-3 polyunsaturated fatty acids in the human diet. Meat Science 74, 209218.
Goering, HK and Van Soest, PJ 1970. Forage fiber analyses (apparatus, reagents, procedures and some applications), Agriculture Handbook No. 379. Agricultural Research Service, United States Department of Agriculture, Washington, DC, USA. pp. 1–20.
Gomez, FE, Bauman, DE, Ntambi, JM and Fox, BG 2003. Effects of sterculic acid on stearoyl-CoA desaturase in differentiating 3T3-L1 adipocytes. Biochemical and Biophysical Research Communications 300, 316326.
Herdmann, A, Martin, J, Nuernberg, G, Dannenberger, D and Nuernberg, K 2010. Effect of dietary n-3 and n-6 PUFA on lipid composition of different tissues of German Holstein bulls and the fate of bioactive fatty acids during processing. Journal of Agricultural and Food Chemistry 58, 83148321.
Hiller, B, Herdmann, A and Nuernberg, K 2011. Dietary n-3 fatty acids significantly suppress lipogenesis in bovine muscle and adipose tissue: a functional genomics approach. Lipids 46, 557567.
Huerta-Leidenz, NO, Savell, JW, Lunt, DK, Baker, JF, Pelton, LS and Smith, SB 1993. Comparison of the fatty acid composition of subcutaneous adipose tissue from mature Brahman and Hereford Cows. Journal of Animal Science 71, 625630.
Kucuk, O, Hess, BW and Rule, DC 2004. Soybean oil supplementation of a high-concentrate diet does not affect site and extent of organic matter, starch, neutral detergent fiber, or nitrogen digestion, but influences both ruminal metabolism and intestinal flow of fatty acids in limit-fed lambs. Journal of Animal Science 82, 29852994.
Laborde, FL, Mandell, IB, Tosh, JJ, Wilton, JW and Buchanan-Smith, JG 2001. Breed effects on growth performance, carcass characteristics, fatty acid composition, and palatability attributes in finishing steers. Journal of Animal Science 79, 355365.
Marchello, JA, Cramer, DA and Miller, LG 1967. Effects of ambient temperature on certain ovine fat characteristics. Journal of Animal Science 26, 294297.
Mir, PS, Dugan, MER, He, ML, Entz, T and Yip, B 2008. Effects of dietary sunflower seeds and tylosin phosphate on production parameters, carcass characteristics, fatty acid composition, and liver abscess incidence in crossbred steers. Journal of Animal Science 86, 31253136.
Moore, JA, Swingle, RS and Hale, WH 1986. Effects of whole cottonseed, cottonseed oil, or animal fat on digestibility of wheat straw diets by steers. Journal of Animal Science 63, 12671273.
Ntambi, JM 1999. Regulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol. Journal of Lipid Research 40, 15491558.
Ntambi, JM and Miyazakib, M 2004. Regulation of stearoyl-CoA desaturases and role in metabolism. Progress in Lipid Research 43, 91104.
Perry, D, Nicholls, PJ and Thompson, JM 1998. The effect of sire breed on the melting point and fatty acid composition of subcutaneous fat in steers. Journal of Animal Science 76, 8795.
Raes, K, De Smet, S and Demeyer, D 2001. Effect of double-muscling in Belgian Blue young bulls on the intramuscular fatty acid composition with emphasis on conjugated linoleic acid and polyunsaturated fatty acids. Animal Science 73, 253260.
Raes, K, De Smet, S and Demeyer, D 2004. Effect of dietary fatty acids on incorporation of long chain polyunsaturated fatty acids, and conjugated linoleic acid in lamb, beef and pork meat: a review. Animal Feed Science and Technology 113, 199221.
Raes, K, Fievez, V, Chow, TT, Ansorena, D, Demeyer, D and De Smet, S 2004. Effect of diet and dietary fatty acids on the transformation and incorporation of C18 fatty acids in double-muscled young bulls. Journal of Agricultural and Food Chemistry 52, 60356041.
Schmitz, G and Ecker, J 2008. The opposing effects of n-3 and n-6 fatty acids. Progress in Lipid Research 47, 147155.
Scollan, ND, Dannenberger, D, Nuernberg, K, Richardson, I, MacKintosh, S, Hocquette, JF and Moloney, A 2014. Enhancing the nutritional and health value of beef lipids and their relationship with meat quality. Meat Science 97, 384394.
Sessler, AM, Kaur, N, Palta, JP and Ntambi, M 1996. Regulation of stearoyl-CoA desaturase 1 mRNA stability by polyunsaturated fatty acids in 3T3-L1 adipocytes. The Journal of Biological Chemistry 271, 2985429858.
Sevi, A, Rotunno, T, DiCaterina, R and Muscio, A 2002. Fatty acid composition of ewe milk as affected by solar radiation and high ambient temperature. Journal of Dairy Research 69, 181194.
Shingfield, KJ, Bonnet, M and Scollan, ND 2013. Recent developments in altering the fatty acid composition of ruminant-derived foods. Animal 7 (s1), 132162.
Tanghe, S, Millet, S and De Smet, S 2013. Echium oil and linseed oil as alternatives for fish oil in the maternal diet: blood fatty acid profiles and oxidative status of sows and piglets. Journal of Animal Science 91, 32533264.
Velliquette, RA, Gillies, PJ, Kris-Etherton, PM, Green, JW, Zhao, G and Vanden Heuvel, JP 2009. Regulation of human stearoyl-CoA desaturase by omega 3 and omega 6 fatty acids: implications for dietary management of elevated serum triglycerides. Journal of Clinical Lipidology 3, 281288.
Wang, J, Duangjinda, M, Vajrabukka, C and Katawatin, S 2014. Differences of skin morphology in Bos taurus, Bos indicus and their crossbreds. International Journal of Biometeorology 58, 10871094.


Related content

Powered by UNSILO

Effect of whole cottonseed v. sunflower seed on the fatty acid profile of subcutaneous fat, longissimus dorsi and blood of Thai Native and Holstein bulls

  • W. Polviset (a1), J. T. Schonewille (a2), H. Everts (a2), C. Wachirapakorn (a1), C. Yuangklang (a3), E. Claeys (a4) and S. De Smet (a4)...


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.