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Effect of the addition of IGF-I and vitamin E to stored boar semen

  • M. F. B. Mendez (a1), M. G. Zangeronimo (a1), L. G. P. Rocha (a1), B. G. Faria (a1), B. A. Pereira (a1), C. D. Fernandes (a1), B. R. Chaves (a1), L. D. S. Murgas (a1) and R. V. Sousa (a1)...


The objective of this study was to evaluate the addition of IGF-I to pig insemination doses stored at 15°C, in conjunction with the addition of different amounts of vitamin E (α-tocopherol). Semen samples (n = 12) from four boars were treated by the addition of different concentrations of vitamin E, ranging up to 400 μg/ml. Immediately after processing and after the doses had been stored at 15°C for 24 or 72 h, samples were warmed at 37°C and 30 ng/ml of IGF-I was added. The assessments were made after 10 and 120 min of IGF-I addition. There was a minor effect of the vitamin E added before cooling and IGF-I added after storage on sperm quality. The addition of 400 μg/ml of vitamin E to diluted semen reduced (P < 0.01) the malondialdehyde (MDA) production in boar semen stored at 15°C for 72 h, regardless of the addition of IGF-I as additive during a 120 min incubation period at 37°C. In these conditions, IGF-I also reduced (P < 0.05) the MDA production in semen samples without addition of vitamin E. IGF-I in the presence of vitamin E reduced (P = 0.03) the glucose intake in freshly diluted boar semen samples before cooling. It was concluded that the addition of 400 μg/ml of vitamin E reduces the MDA production in boar semen stored at 15°C for 72 h, regardless of the presence of IGF-I additive. The addition of IGF-I in doses stored for 72 h with vitamin E ensures higher sperm motility after 120 min of incubation at 37°C.


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Effect of the addition of IGF-I and vitamin E to stored boar semen

  • M. F. B. Mendez (a1), M. G. Zangeronimo (a1), L. G. P. Rocha (a1), B. G. Faria (a1), B. A. Pereira (a1), C. D. Fernandes (a1), B. R. Chaves (a1), L. D. S. Murgas (a1) and R. V. Sousa (a1)...


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