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Effect of the addition of IGF-I and vitamin E to stored boar semen

  • M. F. B. Mendez (a1), M. G. Zangeronimo (a1), L. G. P. Rocha (a1), B. G. Faria (a1), B. A. Pereira (a1), C. D. Fernandes (a1), B. R. Chaves (a1), L. D. S. Murgas (a1) and R. V. Sousa (a1)...

Abstract

The objective of this study was to evaluate the addition of IGF-I to pig insemination doses stored at 15°C, in conjunction with the addition of different amounts of vitamin E (α-tocopherol). Semen samples (n = 12) from four boars were treated by the addition of different concentrations of vitamin E, ranging up to 400 μg/ml. Immediately after processing and after the doses had been stored at 15°C for 24 or 72 h, samples were warmed at 37°C and 30 ng/ml of IGF-I was added. The assessments were made after 10 and 120 min of IGF-I addition. There was a minor effect of the vitamin E added before cooling and IGF-I added after storage on sperm quality. The addition of 400 μg/ml of vitamin E to diluted semen reduced (P < 0.01) the malondialdehyde (MDA) production in boar semen stored at 15°C for 72 h, regardless of the addition of IGF-I as additive during a 120 min incubation period at 37°C. In these conditions, IGF-I also reduced (P < 0.05) the MDA production in semen samples without addition of vitamin E. IGF-I in the presence of vitamin E reduced (P = 0.03) the glucose intake in freshly diluted boar semen samples before cooling. It was concluded that the addition of 400 μg/ml of vitamin E reduces the MDA production in boar semen stored at 15°C for 72 h, regardless of the presence of IGF-I additive. The addition of IGF-I in doses stored for 72 h with vitamin E ensures higher sperm motility after 120 min of incubation at 37°C.

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Alvarez, JG, Storey, BT 1987. Spontaneous lipid peroxidation in rabbit epididymal spermatozoa: its effect on sperm motility. Biology of Reproduction 27, 11021108.
Ball, BA, Medina, V, Gravance, CG, Baumber, J 2001. Effect of antioxidants on preservation of motility, viability and acrosomal integrity of equine spermatozoa during storage at 5°C. Theriogenology 56, 577589.
Baronos, S 1971. Seminal carbohydrate in boar and stallion. Journal of Reproduction and Fertility 24, 303305.
Blom, E 1950. A one-minute live-dead sperm stain by means of eosin–nigrosin. Fertility and Sterility 1, 176177.
Breininger, E, Beorlegui, NB, O'Flaherty, CM, Beconi, MT 2005. Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen. Theriogenology 63, 21262135.
Cerolini, S, Maldjian, A, Surai, P, Noble, R 2000. Viability, susceptibility to peroxidation and fatty acid composition of boar semen during liquid storage. Animal Reproduction Science 58, 99111.
Dalvit, GC, Cetica, PD, Beconi, MT 1998. Effect of α-tocopherol and ascorbic acid on bovine in vitro fertilization. Theriogenology 49, 619627.
Erenpreiss, J, Spano, M, Erenpreisa, A, Bungum, A, Giwercman, A 2006. Sperm chromatin structure and male fertility: biological and clinical aspects. American Journal of Andrology 8, 1129.
Gancarczyk, M, Kuklińska, M, Sadowska, J, Strzeżek, J, Bilińska, B 2006. Aromatization and antioxidant capacity in the testis of seasonally breeding bank voles: effects of LH, PRL, and IGF-l. Theriogenology 65, 13761391.
Henricks, DM, Koubae, AJ, Lackey, BR, Boone, WR, Gray, SL 1998. Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. Biology of Reproduction 59, 330337.
Hirai, M, Boersma, A, Hoeflich, A, Wolf, E, Foll, J, Aumuller, TR, Braun, J 2001. Objectively measured sperm motility and sperm head morphometry in boars (Sus scrofa): relation to fertility and seminal plasma growth factors. Journal of Andrology 22, 104110.
Johannisson, A, Wallgren, M, Rodriguez-Martinez, H 2003. Antioxidant supplementation in vitro improves boar sperm motility and mitochondrial membrane potential after cryopreservation of different fractions of the ejaculate. Animal Reproduction Science 78, 8598.
Lackey, BR, Gray, SL, Henricks, DM 2002. Measurement of leptin and insulin-like growth factor-I in seminal plasma from different species. Physiology Research 51, 309311.
Lewis, SE, Aitken, RJ 2005. DNA damage to spermatozoa has impacts on fertilization and pregnancy. Cell Tissue Research 322, 3341.
Macpherson, ML, Simmen, RCM, Simmen, FA, Hernandez, J, Sheerin, BR, Varner, DD, Loomis, P, Cadario, ME, Miller, CD, Brinsko, SP, Rigby, S, Blanchard, TL 2002. Insulin-like growth factor-l and insulin-like growth factor binding protein-2 and -5 in equine seminal plasma: association with sperm characteristics and fertility. Biology of Reproduction 67, 648654.
Minelli, A, Liguori, L, Collodel, G, Lattaioli, P, Castellini, C 2011. Effects of the purified IGF-I complex on the capacitation and acrosome reaction of rabbit spermatozoa. Journal of Experimental Zoology 290, 311317.
Nabil-Aziz, MD, Saleh, RA, Sharma, RK, Lewis-Jones, I, Esfandiari, N, Thomas, AJ Jr, Agarwal, A 1999. Novel association between sperm reactive oxygen species production, sperm morphological defects, and the sperm deformity index. Fertility and Sterility 2, 484495.
O'Flaherty, C, Beconi, M, Beorlegui, N 1997. Effect of natural antioxidants, superoxide dismutase and hydrogen peroxide on capacitation of frozen-thawed bull spermatozoa. Andrologia 29, 269275.
Selvaraju, S, Reddy, IJ, Nandi, S, Rao, SBN, Ravindra, JP 2009. Influence of IGF-I on buffalo (Bubalus bubalis) spermatozoa motility, membrane integrity, lipid peroxidation and fructose uptake in vitro. Animal Reproduction Science 113, 6070.
Silva, DM, Zangeronimo, MG, Murgas, LDS, Rocha, LGP, Chaves, BR, Pereira, BA, Cunha, ECP 2011. Addition of IGF-l to storage-cooled boar semen and its effects on sperm quality. Growth Hormone & IGF Research 21, 325330.
Wolf, R, Wolf, D, Ruocco, V 1998. Vitamin E: the radical protector. Journal of the European Academy of Dermatology and Venereology 10, 103117.

Keywords

Effect of the addition of IGF-I and vitamin E to stored boar semen

  • M. F. B. Mendez (a1), M. G. Zangeronimo (a1), L. G. P. Rocha (a1), B. G. Faria (a1), B. A. Pereira (a1), C. D. Fernandes (a1), B. R. Chaves (a1), L. D. S. Murgas (a1) and R. V. Sousa (a1)...

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