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Effect of low-protein amino acid-supplemented diets on the growth performance, gut morphology, organ weights and digesta characteristics of weaned pigs

Published online by Cambridge University Press:  01 October 2008

F. O. Opapeju ,
M. Rademacher ,
G. Blank  and
C. M. Nyachoti
F. O. Opapeju
Affiliation:
Department of Animal Science, University of Manitoba, Winnipeg, MB, Canada R3T 2N2
M. Rademacher
Affiliation:
Evonik Industries, Rodenbacher Chaussee 4, 63457 Hanau-Wolfgang, Germany
G. Blank
Affiliation:
Department of Food Science, University of Manitoba, Winnipeg, MB, Canada R3T 2N2
C. M. Nyachoti*
Affiliation:
Department of Animal Science, University of Manitoba, Winnipeg, MB, Canada R3T 2N2
*
E-mail: martin_nyachoti@umanitoba.ca
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Abstract

A 21-day study was conducted to determine whether isoleucine might limit the performance of piglets fed low-crude protein (CP), amino acid (AA)-supplemented diets and to investigate the potential benefits of low-CP diets on gastrointestinal health in weaned pigs. Ninety-six piglets (initial BW = 6.44 ± 0.14 kg), housed four per pen, were randomly assigned to one of four diets, resulting in six replicate pens per diet. Dietary treatments were as follows: (1) 210 g/kg CP diet, (2) 190 g/kg CP diet deficient in isoleucine, (3) 190 g/kg CP diet supplemented with crystalline isoleucine up to the level in the 210 g/kg CP diet and (4) 170 g/kg CP diet supplemented with isoleucine and valine on the ideal protein ratio basis (60% and 70% relative to lysine, respectively). Pigs were allowed to adapt to the new environment for 4 days before the experiment commenced. Overall, pigs fed the 210 g/kg CP diet had higher (P < 0.05) average daily gain and lower (P < 0.05) feed : gain ratio compared with those fed the other diets. The faecal consistency score of pigs fed the 210 g/kg CP diet was higher (P < 0.05) than those fed the other diets. Pigs fed the 170 g/kg diet had lower (P=0.02) small intestine weight than those fed the 210 g/kg CP diet. Pigs fed the 210 g/kg CP diet had deeper (P < 0.05) crypt in the duodenum and ileum and higher (P < 0.05) ammonia N concentration in caecal digesta than those fed the other diets. There were no effects of diet on microbial population and volatile fatty acid concentration in the caecal digesta except for propionic acid whose concentration was higher (P < 0.05) for pigs fed the 170 g/kg diet than those fed the 190+isoleucine and the 210 g/kg CP diets. The results indicate that the low-CP, AA-supplemented diet reduced crypt hypertrophy, ammonia N concentration in the caecal digesta, small intestine weight and the performance of piglets. Also, the results of the current study were inconclusive with respect to whether isoleucine may limit the performance of pigs fed a low-CP, AA-supplemented diet.

Keywords

amino acidsdietary proteingrowth performanceintestinal morphologyweaned pig
Type
Full Paper
Information
animal , Volume 2 , Issue 10 , October 2008 , pp. 1457 - 1464
Copyright
Copyright © The Animal Consortium 2008

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References

Bach Knudsen, KE 1997. Carbohydrate and lignin contents of plant materials used in animal feeding. Animal Feed Science and Technology 67, 319–338.CrossRefGoogle Scholar
Ball, RO, Aherne, FX 1987. Influence of dietary nutrient density, level of feed intake and weaning age on young pigs. II. Apparent nutrient digestibility and incidence and severity of diarrhea. Canadian Journal of Animal Science 67, 1105–1115.CrossRefGoogle Scholar
Boisen, S, Hvelplund, T, Weisbjerg, MR 2000. Ideal amino acid profiles as a basis for feed protein evaluation. Livestock Production Sciences 64, 239–251.CrossRefGoogle Scholar
Canadian Council on Animal Care 1993. Guide to care and use of experimental animals. V.I. CCAC, Ottawa, ON, Canada.Google Scholar
Dunsford, BR, Knabe, DA, Haensly, WE 1989. Effect of dietary soybean meal on the microscopic anatomy of the small intestine in the early-weaned pig. Journal of Animal Science 67, 1855–1863.CrossRefGoogle ScholarPubMed
Erwin, ES, Marco, GJ, Emery, EM 1961. Volatile fatty acids analysis of blood and rumen fluid by gas chromatography. Journal of Dairy Science 44, 1768–1771.CrossRefGoogle Scholar
Figueroa, JL, Lewis, AJ, Miller, PS, Fischer, RL, Gómez, RS, Diedrichsen, RM 2002. Nitrogen metabolism and growth performance of gilts fed standard corn–soybean meal diets or low-crude protein, amino acid supplemented diets. Journal of Animal Science 80, 2911–2919.CrossRefGoogle ScholarPubMed
Gu, X, Li, D 2004. Effect of dietary crude protein level on villous height morphology, immune status and histochemistry parameters of digestive tract in weaning piglets. Animal Feed Science and Technology 114, 113–126.CrossRefGoogle Scholar
Jensen, BB 1998. The impact of feed addictives on the microbial ecology of the gut in young pigs. Journal of Animal Feed Science and Technology 7, 45–64.CrossRefGoogle Scholar
Jiang, R, Chang, X, Stoll, B, Fan, MZ, Arthington, J, Weaver, E, Campbell, J, Burrin, DG 2000. Dietary plasma protein reduces small intestinal growth and lamina propria cell density in early weaned pigs. Journal of Nutrition 130, 21–26.CrossRefGoogle ScholarPubMed
Kerr, BJ, Kidd, MT, Cuaron, JA, Bryant, KL, Parr, TM, Maxwell, CV, Campbell, JM 2004. Isoleucine requirements and ratios in starting (7 to 11 kg) pigs. Journal of Animal Science 82, 2333–2342.CrossRefGoogle ScholarPubMed
Le Bellego, L, Noblet, J 2002. Performance and utilization of dietary energy and amino acids in piglets fed low protein diets. Livestock Production Science 76, 45–58.CrossRefGoogle Scholar
Leibbrandt, VD, Ewan, RC, Speer, VC, Zimmerman, DR 1975. Effect of weaning and age at weaning on baby pig performance. Journal of Animal Science 40, 1077–1080.CrossRefGoogle Scholar
Li, DF, Nelssen, JL, Reddy, PG, Blecha, F, Hancock, JD, Allee, GL, Goodband, RD, Klemm, RD 1990. Transient hypersensitivity to soybean meal in the early-weaned pig. Journal of Animal Science 68, 1790–1799.CrossRefGoogle ScholarPubMed
Li, DF, Nelssen, JL, Reddy, PG, Blecha, F, Klemm, RD, Giesting, DW, Hancock, JD, Allee, GL, Goodband, RD 1991. Measuring suitability of soybean products for early-weaned pigs with immunological criteria. Journal of Animal Science 69, 3299–3307.CrossRefGoogle ScholarPubMed
Lin, HC, Visek, WJ 1991. Colon mucosal cell damage by ammonia in rats. Journal of Nutrition 121, 887–893.CrossRefGoogle ScholarPubMed
National Research Council 1998. Nutrient requirements of swine, 10th revised edition.National Academy Press, Washington, DC, USA.Google Scholar
Nollet, E, Deprez, P, van Driessche, E, Muylle, E 1999. Protection of just weaned pigs against infection with F18+E. coli by non-immune plasma powder. Veterinary Microbiology 65, 37–45.CrossRefGoogle Scholar
Novozamsky, I, van Eck, R, Ch. van Schouwenburg, J, Walinga, I 1974. Total nitrogen determination in plant material by means of the indophenol-blue method. Netherlands Journal of Agricultural Science 22, 3–5.CrossRefGoogle Scholar
Nunez, MC, Bueno, JD, Ayudarte, MV, Almendros, A, Rios, A, Suarez, A, Gil, A 1996. Dietary restriction induces biochemical and morphometric changes in small intestine of nursing piglets. Journal of Nutrition 126, 933–944.CrossRefGoogle ScholarPubMed
Nyachoti, CM, Omogbenigun, FO, Rademacher, M, Blank, G 2006. Performance responses and indicators of gastrointestinal health in early-weaned pigs fed low-protein amino acid-supplemented diets. Journal of Animal Science 84, 125–134.CrossRefGoogle ScholarPubMed
Owusu-Asiedu, A, Baidoo, SK, Nyachoti, CM, Marquardt, RR 2002. Response of early-weaned pigs to spray-dried porcine or animal plasma-based diets supplemented with egg-yolk antibodies against enterotoxigenic E. coli. Journal of Animal Science 80, 2895–2903.CrossRefGoogle ScholarPubMed
Pierce, KM, Callan, JJ, McCarthy, P, O’Doherty, JV 2007. The interaction between lactose level and crude protein concentration on piglet postweaning performance, nitrogen metabolism, selected fecal microbial populations and fecal volatile fatty acid concentration. Animal Feed Science and Technology 132, 267–282.CrossRefGoogle Scholar
Rademacher, M, Sauer, WC, Jansman, AJM 2000. Standardized ileal digestibility of amino acids in pigs. The new system. Degussa-Hüls, Germany.Google Scholar
Stein, HH, Kil, DY 2006. Reduced use of antibiotic growth promoters in diets fed to weanling pigs: dietary tools, part 2. Animal Biotechnology 17, 217–231.CrossRefGoogle ScholarPubMed
Tang, M, Laarveld, B, VanKessel, AG, Hamilton, DL, Estrada, A, Patient, JF 1999. Effect of segregated early weaning on postweaning small intestinal development in pigs. Journal of Animal Science 77, 3191–3200.CrossRefGoogle ScholarPubMed
Van Dijk, AJ, Everts, H, Nabuurs, MJA, Margry, RJCF, Beynen, AC 2001. Growth performance of weanling pigs fed spray-dried animal plasma: a review. Livestock Production Science 68, 263–274.CrossRefGoogle Scholar
Wellock, IJ, Houdijk, JGM, Edwards, SA, Kyriazakis, I 2006a. Too much of a good thing – protein, gut health and performance. Pig Journal 57, 158–172.Google Scholar
Wellock, IJ, Fortomaris, PD, Houdijk, JGM, Kyriazakis, I 2006b. The effect of dietary protein supply on the performance and risk of post-weaning enteric disorders in newly weaned pigs. Animal Science 82, 327–335.CrossRefGoogle Scholar