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The effect of dietary addition of nitrate or increase in lipid concentrations, alone or in combination, on performance and methane emissions of beef cattle

  • C.-A. Duthie (a1), S. M. Troy (a1), J. J. Hyslop (a2), D. W. Ross (a1), R. Roehe (a1) and J. A. Rooke (a1)...

Abstract

Adding nitrate to or increasing the concentration of lipid in the diet are established strategies for reducing enteric methane (CH4) emissions, but their effectiveness when used in combination has been largely unexplored. This study investigated the effect of dietary nitrate and increased lipid included alone or together on CH4 emissions and performance traits of finishing beef cattle. The experiment was a 2×4 factorial design comprising two breeds (cross-bred Aberdeen Angus (AAx) and cross-bred Limousin (LIMx) steers) and four dietary treatments (each based on 550 g forage : 450 g concentrate/kg dry matter (DM)). The four dietary treatments were assigned according to a 2×2 factorial design where the control treatment contained rapeseed meal as the main protein source, which was replaced either with nitrate (21.5 g nitrate/kg DM); maize distillers dark grains (MDDG, which increased diet ether extract from 24 to 37 g/kg DM) or both nitrate and MDDG. Steers (n=20/dietary treatment) were allocated to each of the four treatments in equal numbers of each breed with feed offered ad libitum. After 28 days adaptation to dietary treatments, individual animal intake, performance and feed efficiency were recorded for 56 days. Thereafter, CH4 emissions were measured over 13 weeks (six steers/week). Increasing dietary lipid did not adversely affect animal performance and showed no interactions with dietary nitrate. In contrast, addition of nitrate to diets resulted in poorer live-weight gain (P<0.01) and increased feed conversion ratio (P<0.05) compared with diets not containing nitrate. Daily CH4 output was lower (P<0.001) on nitrate-containing diets but increasing dietary lipid resulted in only a non-significant reduction in CH4. There were no interactions associated with CH4 emissions between dietary nitrate and lipid. Cross-bred Aberdeen Angus steers achieved greater live-weight gains (P<0.01), but had greater DM intakes (P<0.001), greater fat depth (P<0.01) and poorer residual feed intakes (P<0.01) than LIMx steers. Cross-bred Aberdeen Angus steers had higher daily CH4 outputs (P<0.001) but emitted less CH4 per kilogram DM intake than LIMx steers (P<0.05). In conclusion, inclusion of nitrate reduced CH4 emissions in growing beef cattle although the efficacy of nitrate was less than in previous work. When increased dietary lipid and nitrate inclusion were combined there was no evidence of an interaction between treatments and therefore combining different nutritional treatments to mitigate CH4 emissions could be a useful means of achieving reductions in CH4 while minimising any adverse effects.

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Corresponding author

E-mail: john.rooke@sruc.ac.uk

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a

Present address: Blueball, Tullamore, Co. Offaly, Ireland

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References

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Agricultural and Food Research Council 1993. Energy and protein requirements of ruminants. An advisory manual prepared by the AFRC Technical Committee on Responses to Nutrients. CAB International, Wallingford, UK.
Basarab, JA, Price, MA, Aalhus, JL, Okine, EK, Snelling, WM and Lyle, KL 2003. Residual feed intake and body composition in young growing cattle. Canadian Journal of Animal Science 83, 189204.
Bruning-Fann, CS and Kaneene, JB 1993. The effects of nitrate, nitrite, and N-nitroso compounds on animal health. Veterinary and Human Toxicology 35, 237253.
Caetano, M, Wilkes, M, Pitchford, W, Lee, S and Hynd, P 2016. Efficacy of methane-reducing supplements in beef cattle rations. Animal Production Science 56, 276281.
Department of Energy and Climate Change 2016. 2014 UK greenhouse gas emissions final figures – statistical release. Department of Energy and Climate Change, London, UK.
de Raphelis-Soissan, V, Li, L, Godwin, IR, Barnett, MC, Perdok, HB and Hegarty, RS 2014. Use of nitrate and propionibacterium acidipropionici to reduce methane emissions and increase wool growth of Merino sheep. Animal Production Science 54, 18601866.
Duthie, C-A, Rooke, JA, Troy, S, Hyslop, JJ, Ross, DW, Waterhouse, A and Roehe, R 2016. Impact of adding nitrate or increasing the lipid content of two contrasting diets on blood methaemoglobin and performance of two breeds of finishing beef steers. Animal 10, 786795.
El-Zaiat, HM, Araujo, RC, Soltan, YA, Morsy, AS, Louvandini, H, Pires, AV, Patino, HO, Correa, PS and Abdalla, AL 2014. Encapsulated nitrate and cashew nut shell liquid on blood and rumen constituents, methane emission, and growth performance of lambs. Journal of Animal Science 92, 22142224.
Guyader, G, Eugène, M., Doreau, M, Morgavi, DP, Gérard, C, Loncke, C and Martin, C 2015. Nitrate but not tea saponin feed additves decreased enteric methane emissions in nonlactating cows. Journal of Animal Science 93, 53675377.
Guyader, J, Doreau, M, Morgavi, DP, Gerard, C, Loncke, C and Martin, C 2016. Long-term effect of linseed plus nitrate fed to dairy cows on enteric methane emission and nitrate and nitrite residuals in milk. Animal 10, 11731181.
Hegarty, RS, Miller, J, Oelbrandt, N, Li, L, Luijben, JPM, Robinson, DL, Nolan, JV and Perdok, HB 2016. Feed intake, growth, and body and carcass attributes of feedlot steers supplemented with two levels of calcium nitrate or urea. Journal of Animal Science 94, 53725381.
Hristov, AN, Oh, J, Lee, C, Meinen, R, Montes, F, Ott, T, Firkins, J, Rotz, A, Dell, C, Adesogan, A, Yang, W, Tricarico, J, Kebreab, E, Waghorn, G, Dijkstra, J and Oosting, S 2013. Mitigation of greenhouse gas emissions in livestock production – a review of technical options for non-CO2 emissions. In FAO animal production and health paper no. 177 (ed. Gerber PJ, Henderson B and Makkar HPS), pp. 9120. FAO, Rome, Italy.
Klop, G, Hatew, B, Bannink, A and Dijkstra, J 2016. Feeding nitrate and docosahexaenoic acid affects enteric methane production and milk fatty acid composition in lactating dairy cows. Journal of Dairy Science 99, 11611172.
Lee, C, Araujo, R, Koenig, K and Beauchemin, K 2015. Effects of encapsulated nitrate on enteric methane production and nitrogen and energy utilization in beef heifers. Journal of Animal Science 93, 23912404.
Lee, C and Beauchemin, KA 2014. A review of feeding supplementary nitrate to ruminant animals: nitrate toxicity, methane emissions, and production performance. Canadian Journal of Animal Science 94, 557570.
Leng, RA 2014. Interactions between microbial consortia in biofilms: a paradigm shift in rumen microbial ecology and enteric methane mitigation. Animal Production Science 54, 519543.
Li, L, Silveira, CI, Nolan, JV, Godwin, I, Leng, R and Hegarty, R 2013. Effect of added dietary nitrate and elemental sulfur on wool growth and methane emission of Merino lambs. Animal Production Science 53, 11951201.
Martin, C, Morgavi, DP and Doreau, M 2010. Methane mitigation in ruminants: from microbe to the farm scale. Animal 4, 351365.
Ministry of Agriculture Fisheries and Food 1992. Analysis of agricultural materials, 2nd edition. Her Majesty’s Stationary Office, London, UK.
Newbold, JR, van Zijderveld, SM, Hulshof, RBA, Fokkink, WB, Leng, RA, Terencio, P, Powers, WJ, van Adrichem, PSJ, Paton, ND and Perdok, HB 2014. The effect of incremental levels of dietary nitrate on methane emissions in Holstein steers and performance in Nelore bulls. Journal of Animal Science 92, 50325040.
Noziere, P, Glasser, F and Sauvant, D 2011. In vivo production and molar percentages of volatile fatty acids in the rumen: a quantitative review by an empirical approach. Animal 5, 403414.
Patra, AK 2013. The effect of dietary fats on methane emissions, and its other effects on digestibility, rumen fermentation and lactation performance in cattle: a meta-analysis. Livestock Science 155, 244254.
Patra, AK 2014. A meta-analysis of the effect of dietary fat on enteric methane production, digestibility and rumen fermentation in sheep, and a comparison of these responses between cattle and sheep. Livestock Science 162, 97103.
Ramin, M and Huhtanen, P 2013. Development of equations for predicting methane emissions from ruminants. Journal of Dairy Science 96, 24762493.
Rooke, JA, Borman, AJ and Armstrong, DG 1990. The effect of inoculation with Lactobacillus plantarum on fermentation in laboratory silos of herbage low in water-soluble carbohydrate. Grass and Forage Science 45, 143152.
Rooke, JA, Miller, G, Flockhart, J, McDowell, M and MacLeod, M 2016. Nutritional strategies to reduce enteric methane emissions. climateXchange. Retrieved on 17 March 2017 from http://www.climatexchange.org.uk/reducing-emissions/emissions-livestock-production/.
Rooke, JA, Wallace, RJ, Duthie, C-A, McKain, N, de Souza, SM, Hyslop, JJ, Ross, DW, Waterhouse, T and Roehe, R 2014. Hydrogen and methane emissions from beef cattle and their rumen microbial community vary with diet, time after feeding and genotype. British Journal of Nutrition 112, 398407.
Sauvant, D and Giger-Reverdin, S 2009. Modelling of digestive interactions and methane production in ruminants. Productions Animales 22, 375384.
Troy, S, Duthie, CA, Hyslop, J, Roehe, R, Ross, D, Wallace, R, Waterhouse, A and Rooke, J 2015. Effectiveness of nitrate addition and increased oil content as methane mitigation strategies for beef cattle fed two contrasting basal diets. Journal of Animal Science 93, 18151823.
van Zijderveld, S, Gerrits, W, Apajalahti, J, Newbold, J, Dijkstra, J, Leng, R and Perdok, H 2010. Nitrate and sulfate: effective alternative hydrogen sinks for mitigation of ruminal methane production in sheep. Journal of Dairy Science 93, 58565866.
van Zijderveld, SM, Fonken, B, Dijkstra, J, Gerrits, WJJ, Perdok, HB, Fokkink, W and Newbold, JR 2011a. Effects of a combination of feed additives on methane production, diet digestibility, and animal performance in lactating dairy cows. Journal of Dairy Science 94, 14451454.
van Zijderveld, SM, Gerrits, WJJ, Dijkstra, J, Newbold, JR, Hulshof, RBA and Perdok, HB 2011b. Persistency of methane mitigation by dietary nitrate supplementation in dairy cows. Journal of Dairy Science 94, 40284038.
Veneman, JB, Muetzel, S, Hart, KJ, Faulkner, CL, Moorby, JM, Perdok, HB and Newbold, CJ 2015. Does dietary mitigation of enteric methane production affect rumen function and animal productivity in dairy cows? Plos One 10, e0140282.
Yang, CJ, Rooke, JA, Cabeza, I and Wallace, RJ 2016. Nitrate and inhibition of ruminal methanogenesis: microbial ecology, obstacles, and opportunities for lowering methane emissions from ruminant livestock. Frontiers in Microbiology 7, 132.

Keywords

The effect of dietary addition of nitrate or increase in lipid concentrations, alone or in combination, on performance and methane emissions of beef cattle

  • C.-A. Duthie (a1), S. M. Troy (a1), J. J. Hyslop (a2), D. W. Ross (a1), R. Roehe (a1) and J. A. Rooke (a1)...

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