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Dietary phytase and myo-inositol supplementation are associated with distinct plasma metabolome profile in broiler chickens

  • F. Gonzalez-Uarquin (a1), Á. Kenéz (a2), M. Rodehutscord (a1) and K. Huber (a1)


Phytase enzyme is used as a dietary supplement in broiler nutrition to improve phosphorous bioavailability. Phytase deliberates phosphate groups from phytic acid and produces myo-inositol after total dephosphorylation. Myo-inositol is a bioactive compound having beneficial modulatory effects on metabolism in humans. However, it is not well understood if and how phytic acid degradation products, particularly myo-inositol, can modulate metabolism in broiler chicken. The purpose of this study was to investigate effects of dietary supplements of phytase and myo-inositol on the blood plasma metabolome profile of broiler chickens. Broilers were provided a nutrient-adequate control diet or the same diet supplemented with either 3.5 g myo-inositol or 500, 1500 or 3000 units of phytase, per kilogram of feed (grower diet). Broilers were group-housed in floor pens (eight pens per diet) and provided one of the treatment diets for 22 days. Then, blood was collected from one bird per pen, resulting in eight replicated measurements per diet. A targeted metabolomics approach was applied to the heparin plasma. Body weight of the birds was not significantly affected by the treatments. Plasma myo-inositol concentrations were significantly increased by myo-inositol supplementation and phytase supplementation at 500 and 1500 units/kg. Metabolites generally affected by phytase supplementation belonged to the groups of acyl-carnitines, phosphatidylcholines, sphingomyelins, lysophosphatidylcholine, biogenic amines and amino acids. Compared to the control diet, phytase supplements had significantly higher plasma concentrations of kynurenine and creatinine, but lower concentrations of histamine and cis-4-hydroxyproline. Myo-inositol supplementation significantly increased plasma concentrations of dopamine and serotonine. While some metabolites were similarly affected by myo-inositol and phytase supplementation, others were distinctly differently affected. We conclude that myo-inositol, either as a directly added supplement or indirectly released from phytate upon phytase supplementation, can affect specific metabolic pathways. Additional effects found on phytase supplementation may be related to intermediary phytate degradation products. Results are indicative for innovative hypothesis to be tested in future experiments, for instance, with regard to relationships between phytase or myo-inositol supplements and bird immunity or behaviour.


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These two authors contributed equally to this work.



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Angeloff, LG, Skoryna, SC and Henderson, IWD 2009. Effects of the hexahydroxyhexane myoinositol on bone uptake of radiocalcium in rats: effect of inositol and vitamin D2 on bone uptake of 45Ca in rats. Acta Pharmacologica et Toxicologica 40, 209215.
Aouameur, R, Da Cal, S, Bissonnette, P, Coady, MJ and Lapointe, J-Y 2007. SMIT2 mediates all myo-inositol uptake in apical membranes of rat small intestine. American Journal of Physiology. Gastrointestinal and Liver Physiology 293, G13001307.
Bedford, MR and Walk, CL (eds. Walk, CL, Kuhn, I, Stein, HH, Kidd, MT and Rodehutscord, M 2016. Reduction of phytate to tetrakisphosphate (IP4) to triphosphate (IP3), or perhaps even lower, does not remove its antinutritive properties. In Phytate destruction – consequences for precision animal nutrition (eds. ), pp. 4552. Wageningen Academic Publishers, Wageningen, The Netherlands.
Buzała, M, Janicki, B and Czarnecki, R 2015. Consequences of different growth rates in broiler breeder and layer hens on embryogenesis, metabolism and metabolic rate: a review. Poultry Science 94, 728733.
Chong, J Wishart, DS Xia, J 2019. Using metaboanalyst 4.0 for comprehensive and integrative metabolomics data analysis. Current Protocols in Bioinformatics 68, e86.
Cowieson, AJ, Ptak, A, Mackowiak, P, Sassek, M, Pruszynska-Oszmalek, E, Zyla, K, Swiatkiewicz, S, Kaczmarek, S and Jozefiak, D 2013. The effect of microbial phytase and myo-inositol on performance and blood biochemistry of broiler chickens fed wheat/corn-based diets. Poultry Science 92, 21242134.
Cowieson, AJ, Aureli, R, Guggenbuhl, P and Fru-Nji, F 2015. Possible involvement of myo inositol in the physiological response of broilers to high doses of microbial phytase. Animal Production Science 55, 710719.
Croze, ML and Soulage, CO 2013. Potential role and therapeutic interests of myo-inositol in metabolic diseases. Biochimie 95, 18111827.
Dang, NT, Mukai, R, Yoshida, K and Ashida, H 2010. D-pinitol and myo-inositol stimulate translocation of glucose transporter 4 in skeletal muscle of C57BL/6 mice. Bioscience, Biotechnology, and Biochemistry 74, 10621067.
de Haas, EN and van der Eijk, JAJ 2018. Where in the serotonergic system does it go wrong? Unravelling the route by which the serotonergic system affects feather pecking in chickens. Neuroscience & Biobehavioral Reviews 95, 170188.
Farhadi, D, Karimi, A, Sadeghi, G, Rostamzadeh, J and Bedford, MR 2017. Effects of a high dose of microbial phytase and myo-inositol supplementation on growth performance, tibia mineralization, nutrient digestibility, litter moisture content, and foot problems in broiler chickens fed phosphorus-deficient diets. Poultry Science 96, 36643675.
Floegel, A, Stefan, N, Yu, Z, Mühlenbruch, K, Drogan, D, Joost, HG, Fritsche, A, Häring, HU, Hrabe de Angelis, M, Peters, A, Roden, M, Prehn, C, Wang-Sattler, R, llig, T, Schulze, MB, Adamski, J, Boeing, H and Pischon, T 2012. Identification of serum metabolites associated with risk of type 2 diabetes using a targeted metabolomic approach. Diabetes 62, 639648.
Gautier, AE, Walk, CL and Dilger, RN 2018. Effects of a high level of phytase on broiler performance, bone ash, phosphorus utilization, and phytate dephosphorylation to inositol. Poultry Science 97, 211218.
Goldansaz, SA, Guo, AC, Sajed, T, Steele, MA, Plastow, GS and Wishart, DS 2017. Livestock metabolomics and the livestock metabolome: a systematic review. PLoS One 12, e0177675.
Harry, EG, Tucker, JF and Laursen-Jones, AP 1975. The role of histamine and fish meal in the incidence of gizzard erosion and pro-ventricular abnormalities in the fowl. British Poultry Science 16, 6978.
Harvey, BH, Scheepers, A, Brand, L and Stein, DJ 2001. Chronic inositol increases striatal D(2) receptors but does not modify dexamphetamine-induced motor behavior. Relevance to obsessive-compulsive disorder. Pharmacology Biochemistry and Behavior 68, 245253.
Hofmann, P, Siegert, W, Kenéz, Á, Naranjo, V and Rodehutscord, M 2019. Very low crude protein and varying glycine concentrations in the diet affect growth performance, characteristics of nitrogen excretion, and the blood metabolome of broiler chickens. The Journal of Nutrition 149, 11221132.
Holub, BJ 1986. Metabolism and function of myo-inositol and inositol phospholipids. Annual Review of Nutrition 6, 563597.
Kenéz, Á, Koch, C, Korst, M, Kesser, J, Eder, K, Sauerwein, H and Huber, K 2018a. Different milk feeding intensities during the first 4 weeks of rearing dairy calves: part 3: plasma metabolomics analysis reveals long-term metabolic imprinting in Holstein heifers. Journal of Dairy Science 101, 84468460.
Kenéz, Á, Warnken, T, Feige, K and Huber, K 2018b. Lower plasma trans-4-hydroxyproline and methionine sulfoxide levels are associated with insulin dysregulation in horses. BMC Veterinary Research 14, 146.
Kenéz, Á, Dänicke, S, Rolle-Kampczyk, U, von Bergen, M and Huber, K 2016. A metabolomics approach to characterize phenotypes of metabolic transition from late pregnancy to early lactation in dairy cows. Metabolomics 12, 165.
Kim, JN, Han, SN and Kim, H-K 2014. Phytic acid and myo-inositol support adipocyte differentiation and improve insulin sensitivity in 3T3-L1 cells. Nutrition Research 34, 723731.
Li, P and Wu, G 2018. Roles of dietary glycine, proline, and hydroxyproline in collagen synthesis and animal growth. Amino Acids 50, 2938.
Lu, YP, Reichetzeder, C, Prehn, C, Yin, LH, Yun, C, Zeng, S, Chu, C, Adamski, J and Hocher, B 2018. Cord blood lysophosphatidylcholine 16:1 is positively associated with birth weight. Cellular Physiology and Biochemistry 45, 614624.
Mirnaghizadeh, SV, Zendehdel, M and Babapour, V 2017. Involvement of histaminergic and noradrenergic receptors in the oxytocin-induced food intake in neonatal meat-type chicks. Veterinary Research Communications 41, 5766.
O’Connor, TM, Bourke, JF, Jones, M and Brennan, N 2001. Report of occupational asthma due to phytase and β-glucanase. Occupational and Environmental Medicine 58, 417419.
Pirgozliev, V, Bedford, MR, Rose, SP, Whiting, IM, Oluwatosin, OO, Oso, AO, Oke, FO, Ivanova, SG and Staykova, GP 2017. Phosphorus utilisation and growth performance of broiler chicken fed diets containing graded levels of supplementary myo-inositol with and without exogenous phytase. Journal of World’s Poultry Research 7, 17.
Plows, J, Budin, F, Andersson, R, Mills, V, Mace, K, Davidge, S, Vickers, M, Baker, P, Silva-Zolezzi, I and Stanley, J 2017. The effects of myo-inositol and B and D vitamin supplementation in the db/+ Mouse model of gestational diabetes mellitus. Nutrients 9, 141.
Ramsay, SL Stoeggl, WM Weinberger, KM Graber, A Guggenbichler, W 2007. Apparatus and method for analyzing a metabolite profile. Patent No. EP 1875401 A2. Biocrates Life Sciences AG, Innsbruck, Austria.
Schmeisser, J, Séon, A-A, Aureli, R, Friedel, A, Guggenbuhl, P, Duval, S, Cowieson, AJ and Fru-Nji, F 2017. Exploratory transcriptomic analysis in muscle tissue of broilers fed a phytase-supplemented diet. Journal of Animal Physiology and Animal Nutrition 101, 563575.
Schäfer, N, Yu, Z, Wagener, A, Millrose, MK, Reissmann, M, Bortfeldt, R, Dieterich, C, Adamski, J, Wang-Sattler, R, Illig, T and Brockmann, GA 2014. Changes in metabolite profiles caused by genetically determined obesity in mice. Metabolomics 10, 461472.
Sedlmeier, A, Kluttig, A, Giegling, I, Prehn, C, Adamski, J, Kastenmüller, G and Lacruz, ME 2018. The human metabolic profile reflects macro- and micronutrient intake distinctly according to fasting time. Scientific Reports 8, 12262.
Shajib, MS, Baranov, A and Khan, WI 2017. Diverse effects of gut-derived serotonin in intestinal inflammation. ACS Chemical Neuroscience 8, 920931.
Shastak, Y, Zeller, E, Witzig, M, Schollenberger, M and Rodehutscord, M 2014. Effects of the composition of the basal diet on the evaluation of mineral phosphorus sources and interactions with phytate hydrolysis in broilers. Poultry Science 93, 25482559.
Sommerfeld, V, Künzel, S, Schollenberger, M, Kühn, I and Rodehutscord, M 2018a. Influence of phytase or myo-inositol supplements on performance and phytate degradation products in the crop, ileum, and blood of broiler chickens. Poultry Science 97, 920929.
Sommerfeld, V, Schollenberger, M, Kühn, I and Rodehutscord, M 2018b. Interactive effects of phosphorus, calcium, and phytase supplements on products of phytate degradation in the digestive tract of broiler chickens. Poultry Science 97, 11771188.
Stadtman, ER and Levine, RL 2003. Free radical-mediated oxidation of free amino acids and amino acid residues in proteins. Amino Acids 25, 207218.
Walk, CL, Bedford, MR and Olukosi, OA 2018. Effect of phytase on growth performance, phytate degradation and gene expression of myo-inositol transporters in the small intestine, liver and kidney of 21 day old broilers. Poultry Science 97, 11551162.
Wang, Y, Liu, H, McKenzie, G, Witting, PK, Stasch, J-P, Hahn, M, Changsirivathanathamrong, D, Wu, BJ, Ball, HJ, Thomas, SR, Kapoor, V, Celermajer, DS, Mellor, AL, Keaney, JF, Hunt, NH and Stocker, R 2010. Kynurenine is an endothelium-derived relaxing factor produced during inflammation. Nature Medicine 16, 279285.
Zeller, E, Schollenberger, M, Witzig, M, Shastak, Y, Kühn, I, Hoelzle, LE and Rodehutscord, M 2015. Interactions between supplemented mineral phosphorus and phytase on phytate hydrolysis and inositol phosphates in the small intestine of broilers. Poultry Science 94, 10181029.
Zendehdel, M, Sardari, F, Hassanpour, S, Rahnema, M, Adeli, A and Ghashghayi, E 2017. Serotonin-induced hypophagia is mediated via α 2 and β 2 adrenergic receptors in neonatal layer-type chickens. British Poultry Science 58, 298304.
Zuidhof, MJ, Schneider, BL, Carney, VL, Korver, DR and Robinson, FE 2014. Growth, efficiency, and yield of commercial broilers from 1957, 1978, and 2005. Poultry Science 93, 29702982.
Żyła, K, Duliński, R, Pierzchalska, M, Grabacka, M, Józefiak, D and Świątkiewicz, S 2013. Phytases and myo-inositol modulate performance, bone mineralization and alter lipid fractions in the serum of broilers. Journal of Animal and Feed Sciences 22, 5662.


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Dietary phytase and myo-inositol supplementation are associated with distinct plasma metabolome profile in broiler chickens

  • F. Gonzalez-Uarquin (a1), Á. Kenéz (a2), M. Rodehutscord (a1) and K. Huber (a1)


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