Hostname: page-component-84b7d79bbc-fnpn6 Total loading time: 0 Render date: 2024-07-28T16:57:46.018Z Has data issue: false hasContentIssue false
  • English
  • Français

Dietary phytase and myo-inositol supplementation are associated with distinct plasma metabolome profile in broiler chickens

Published online by Cambridge University Press:  15 October 2019

F. Gonzalez-Uarquin ,
Á. Kenéz Open the ORCID record for Á. Kenéz [Opens in a new window] ,
M. Rodehutscord  and
K. Huber
F. Gonzalez-Uarquin
Affiliation:
Institute of Animal Science, Faculty of Agricultural Sciences, University of Hohenheim, 70593 Stuttgart, Germany
Á. Kenéz
Affiliation:
Department of Infectious Diseases and Public Health, City University of Hong Kong, Hong Kong SAR
M. Rodehutscord
Affiliation:
Institute of Animal Science, Faculty of Agricultural Sciences, University of Hohenheim, 70593 Stuttgart, Germany
K. Huber*
Affiliation:
Institute of Animal Science, Faculty of Agricultural Sciences, University of Hohenheim, 70593 Stuttgart, Germany
*
†E-mail: korinna.huber@uni-hohenheim.de
Get access

Abstract

Phytase enzyme is used as a dietary supplement in broiler nutrition to improve phosphorous bioavailability. Phytase deliberates phosphate groups from phytic acid and produces myo-inositol after total dephosphorylation. Myo-inositol is a bioactive compound having beneficial modulatory effects on metabolism in humans. However, it is not well understood if and how phytic acid degradation products, particularly myo-inositol, can modulate metabolism in broiler chicken. The purpose of this study was to investigate effects of dietary supplements of phytase and myo-inositol on the blood plasma metabolome profile of broiler chickens. Broilers were provided a nutrient-adequate control diet or the same diet supplemented with either 3.5 g myo-inositol or 500, 1500 or 3000 units of phytase, per kilogram of feed (grower diet). Broilers were group-housed in floor pens (eight pens per diet) and provided one of the treatment diets for 22 days. Then, blood was collected from one bird per pen, resulting in eight replicated measurements per diet. A targeted metabolomics approach was applied to the heparin plasma. Body weight of the birds was not significantly affected by the treatments. Plasma myo-inositol concentrations were significantly increased by myo-inositol supplementation and phytase supplementation at 500 and 1500 units/kg. Metabolites generally affected by phytase supplementation belonged to the groups of acyl-carnitines, phosphatidylcholines, sphingomyelins, lysophosphatidylcholine, biogenic amines and amino acids. Compared to the control diet, phytase supplements had significantly higher plasma concentrations of kynurenine and creatinine, but lower concentrations of histamine and cis-4-hydroxyproline. Myo-inositol supplementation significantly increased plasma concentrations of dopamine and serotonine. While some metabolites were similarly affected by myo-inositol and phytase supplementation, others were distinctly differently affected. We conclude that myo-inositol, either as a directly added supplement or indirectly released from phytate upon phytase supplementation, can affect specific metabolic pathways. Additional effects found on phytase supplementation may be related to intermediary phytate degradation products. Results are indicative for innovative hypothesis to be tested in future experiments, for instance, with regard to relationships between phytase or myo-inositol supplements and bird immunity or behaviour.

Keywords

serotonindopaminemetabolomicsphytic acidphosphate
Type
Research Article
Information
animal , Volume 14 , Issue 3 , March 2020 , pp. 549 - 559
Copyright
© The Animal Consortium 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

*

These two authors contributed equally to this work.

References

Angeloff, LG, Skoryna, SC and Henderson, IWD 2009. Effects of the hexahydroxyhexane myoinositol on bone uptake of radiocalcium in rats: effect of inositol and vitamin D2 on bone uptake of 45Ca in rats. Acta Pharmacologica et Toxicologica 40, 209–215.CrossRefGoogle Scholar
Aouameur, R, Da Cal, S, Bissonnette, P, Coady, MJ and Lapointe, J-Y 2007. SMIT2 mediates all myo-inositol uptake in apical membranes of rat small intestine. American Journal of Physiology. Gastrointestinal and Liver Physiology 293, G1300–1307.CrossRefGoogle ScholarPubMed
Bedford, MR and Walk, CL (eds. Walk, CL, Kuhn, I, Stein, HH, Kidd, MT and Rodehutscord, M 2016. Reduction of phytate to tetrakisphosphate (IP4) to triphosphate (IP3), or perhaps even lower, does not remove its antinutritive properties. In Phytate destruction – consequences for precision animal nutrition (eds. ), pp. 45–52. Wageningen Academic Publishers, Wageningen, The Netherlands.CrossRefGoogle Scholar
Buzała, M, Janicki, B and Czarnecki, R 2015. Consequences of different growth rates in broiler breeder and layer hens on embryogenesis, metabolism and metabolic rate: a review. Poultry Science 94, 728–733.CrossRefGoogle ScholarPubMed
Chong, J, Wishart, DS and Xia, J 2019. Using metaboanalyst 4.0 for comprehensive and integrative metabolomics data analysis. Current Protocols in Bioinformatics 68, e86.CrossRefGoogle ScholarPubMed
Cowieson, AJ, Ptak, A, Mackowiak, P, Sassek, M, Pruszynska-Oszmalek, E, Zyla, K, Swiatkiewicz, S, Kaczmarek, S and Jozefiak, D 2013. The effect of microbial phytase and myo-inositol on performance and blood biochemistry of broiler chickens fed wheat/corn-based diets. Poultry Science 92, 2124–2134.CrossRefGoogle ScholarPubMed
Cowieson, AJ, Aureli, R, Guggenbuhl, P and Fru-Nji, F 2015. Possible involvement of myo inositol in the physiological response of broilers to high doses of microbial phytase. Animal Production Science 55, 710–719.CrossRefGoogle Scholar
Croze, ML and Soulage, CO 2013. Potential role and therapeutic interests of myo-inositol in metabolic diseases. Biochimie 95, 1811–1827.CrossRefGoogle ScholarPubMed
Dang, NT, Mukai, R, Yoshida, K and Ashida, H 2010. D-pinitol and myo-inositol stimulate translocation of glucose transporter 4 in skeletal muscle of C57BL/6 mice. Bioscience, Biotechnology, and Biochemistry 74, 1062–1067.CrossRefGoogle ScholarPubMed
de Haas, EN and van der Eijk, JAJ 2018. Where in the serotonergic system does it go wrong? Unravelling the route by which the serotonergic system affects feather pecking in chickens. Neuroscience & Biobehavioral Reviews 95, 170–188.CrossRefGoogle ScholarPubMed
Farhadi, D, Karimi, A, Sadeghi, G, Rostamzadeh, J and Bedford, MR 2017. Effects of a high dose of microbial phytase and myo-inositol supplementation on growth performance, tibia mineralization, nutrient digestibility, litter moisture content, and foot problems in broiler chickens fed phosphorus-deficient diets. Poultry Science 96, 3664–3675.CrossRefGoogle ScholarPubMed
Floegel, A, Stefan, N, Yu, Z, Mühlenbruch, K, Drogan, D, Joost, HG, Fritsche, A, Häring, HU, Hrabe de Angelis, M, Peters, A, Roden, M, Prehn, C, Wang-Sattler, R, llig, T, Schulze, MB, Adamski, J, Boeing, H and Pischon, T 2012. Identification of serum metabolites associated with risk of type 2 diabetes using a targeted metabolomic approach. Diabetes 62, 639–648.CrossRefGoogle ScholarPubMed
Gautier, AE, Walk, CL and Dilger, RN 2018. Effects of a high level of phytase on broiler performance, bone ash, phosphorus utilization, and phytate dephosphorylation to inositol. Poultry Science 97, 211–218.CrossRefGoogle ScholarPubMed
Goldansaz, SA, Guo, AC, Sajed, T, Steele, MA, Plastow, GS and Wishart, DS 2017. Livestock metabolomics and the livestock metabolome: a systematic review. PLoS One 12, e0177675.CrossRefGoogle ScholarPubMed
Harry, EG, Tucker, JF and Laursen-Jones, AP 1975. The role of histamine and fish meal in the incidence of gizzard erosion and pro-ventricular abnormalities in the fowl. British Poultry Science 16, 69–78.CrossRefGoogle Scholar
Harvey, BH, Scheepers, A, Brand, L and Stein, DJ 2001. Chronic inositol increases striatal D(2) receptors but does not modify dexamphetamine-induced motor behavior. Relevance to obsessive-compulsive disorder. Pharmacology Biochemistry and Behavior 68, 245–253.CrossRefGoogle Scholar
Hofmann, P, Siegert, W, Kenéz, Á, Naranjo, V and Rodehutscord, M 2019. Very low crude protein and varying glycine concentrations in the diet affect growth performance, characteristics of nitrogen excretion, and the blood metabolome of broiler chickens. The Journal of Nutrition 149, 1122–1132.CrossRefGoogle ScholarPubMed
Holub, BJ 1986. Metabolism and function of myo-inositol and inositol phospholipids. Annual Review of Nutrition 6, 563–597.CrossRefGoogle ScholarPubMed
Kenéz, Á, Koch, C, Korst, M, Kesser, J, Eder, K, Sauerwein, H and Huber, K 2018a. Different milk feeding intensities during the first 4 weeks of rearing dairy calves: part 3: plasma metabolomics analysis reveals long-term metabolic imprinting in Holstein heifers. Journal of Dairy Science 101, 8446–8460.CrossRefGoogle ScholarPubMed
Kenéz, Á, Warnken, T, Feige, K and Huber, K 2018b. Lower plasma trans-4-hydroxyproline and methionine sulfoxide levels are associated with insulin dysregulation in horses. BMC Veterinary Research 14, 146.CrossRefGoogle ScholarPubMed
Kenéz, Á, Dänicke, S, Rolle-Kampczyk, U, von Bergen, M and Huber, K 2016. A metabolomics approach to characterize phenotypes of metabolic transition from late pregnancy to early lactation in dairy cows. Metabolomics 12, 165.CrossRefGoogle Scholar
Kim, JN, Han, SN and Kim, H-K 2014. Phytic acid and myo-inositol support adipocyte differentiation and improve insulin sensitivity in 3T3-L1 cells. Nutrition Research 34, 723–731.CrossRefGoogle ScholarPubMed
Li, P and Wu, G 2018. Roles of dietary glycine, proline, and hydroxyproline in collagen synthesis and animal growth. Amino Acids 50, 29–38.CrossRefGoogle ScholarPubMed
Lu, YP, Reichetzeder, C, Prehn, C, Yin, LH, Yun, C, Zeng, S, Chu, C, Adamski, J and Hocher, B 2018. Cord blood lysophosphatidylcholine 16:1 is positively associated with birth weight. Cellular Physiology and Biochemistry 45, 614–624.CrossRefGoogle ScholarPubMed
Mirnaghizadeh, SV, Zendehdel, M and Babapour, V 2017. Involvement of histaminergic and noradrenergic receptors in the oxytocin-induced food intake in neonatal meat-type chicks. Veterinary Research Communications 41, 57–66.CrossRefGoogle ScholarPubMed
O’Connor, TM, Bourke, JF, Jones, M and Brennan, N 2001. Report of occupational asthma due to phytase and β-glucanase. Occupational and Environmental Medicine 58, 417–419.CrossRefGoogle ScholarPubMed
Pirgozliev, V, Bedford, MR, Rose, SP, Whiting, IM, Oluwatosin, OO, Oso, AO, Oke, FO, Ivanova, SG and Staykova, GP 2017. Phosphorus utilisation and growth performance of broiler chicken fed diets containing graded levels of supplementary myo-inositol with and without exogenous phytase. Journal of World’s Poultry Research 7, 1–7.Google Scholar
Plows, J, Budin, F, Andersson, R, Mills, V, Mace, K, Davidge, S, Vickers, M, Baker, P, Silva-Zolezzi, I and Stanley, J 2017. The effects of myo-inositol and B and D vitamin supplementation in the db/+ Mouse model of gestational diabetes mellitus. Nutrients 9, 141.CrossRefGoogle Scholar
Ramsay, SL, Stoeggl, WM, Weinberger, KM, Graber, A and Guggenbichler, W 2007. Apparatus and method for analyzing a metabolite profile. Patent No. EP 1875401 A2. Biocrates Life Sciences AG, Innsbruck, Austria.Google Scholar
Schmeisser, J, Séon, A-A, Aureli, R, Friedel, A, Guggenbuhl, P, Duval, S, Cowieson, AJ and Fru-Nji, F 2017. Exploratory transcriptomic analysis in muscle tissue of broilers fed a phytase-supplemented diet. Journal of Animal Physiology and Animal Nutrition 101, 563–575.CrossRefGoogle ScholarPubMed
Schäfer, N, Yu, Z, Wagener, A, Millrose, MK, Reissmann, M, Bortfeldt, R, Dieterich, C, Adamski, J, Wang-Sattler, R, Illig, T and Brockmann, GA 2014. Changes in metabolite profiles caused by genetically determined obesity in mice. Metabolomics 10, 461–472.CrossRefGoogle ScholarPubMed
Sedlmeier, A, Kluttig, A, Giegling, I, Prehn, C, Adamski, J, Kastenmüller, G and Lacruz, ME 2018. The human metabolic profile reflects macro- and micronutrient intake distinctly according to fasting time. Scientific Reports 8, 12262.CrossRefGoogle ScholarPubMed
Shajib, MS, Baranov, A and Khan, WI 2017. Diverse effects of gut-derived serotonin in intestinal inflammation. ACS Chemical Neuroscience 8, 920–931.CrossRefGoogle ScholarPubMed
Shastak, Y, Zeller, E, Witzig, M, Schollenberger, M and Rodehutscord, M 2014. Effects of the composition of the basal diet on the evaluation of mineral phosphorus sources and interactions with phytate hydrolysis in broilers. Poultry Science 93, 2548–2559.CrossRefGoogle ScholarPubMed
Sommerfeld, V, Künzel, S, Schollenberger, M, Kühn, I and Rodehutscord, M 2018a. Influence of phytase or myo-inositol supplements on performance and phytate degradation products in the crop, ileum, and blood of broiler chickens. Poultry Science 97, 920–929.CrossRefGoogle ScholarPubMed
Sommerfeld, V, Schollenberger, M, Kühn, I and Rodehutscord, M 2018b. Interactive effects of phosphorus, calcium, and phytase supplements on products of phytate degradation in the digestive tract of broiler chickens. Poultry Science 97, 1177–1188.CrossRefGoogle ScholarPubMed
Stadtman, ER and Levine, RL 2003. Free radical-mediated oxidation of free amino acids and amino acid residues in proteins. Amino Acids 25, 207–218.CrossRefGoogle ScholarPubMed
Walk, CL, Bedford, MR and Olukosi, OA 2018. Effect of phytase on growth performance, phytate degradation and gene expression of myo-inositol transporters in the small intestine, liver and kidney of 21 day old broilers. Poultry Science 97, 1155–1162.CrossRefGoogle ScholarPubMed
Wang, Y, Liu, H, McKenzie, G, Witting, PK, Stasch, J-P, Hahn, M, Changsirivathanathamrong, D, Wu, BJ, Ball, HJ, Thomas, SR, Kapoor, V, Celermajer, DS, Mellor, AL, Keaney, JF, Hunt, NH and Stocker, R 2010. Kynurenine is an endothelium-derived relaxing factor produced during inflammation. Nature Medicine 16, 279–285.CrossRefGoogle ScholarPubMed
Zeller, E, Schollenberger, M, Witzig, M, Shastak, Y, Kühn, I, Hoelzle, LE and Rodehutscord, M 2015. Interactions between supplemented mineral phosphorus and phytase on phytate hydrolysis and inositol phosphates in the small intestine of broilers. Poultry Science 94, 1018–1029.CrossRefGoogle Scholar
Zendehdel, M, Sardari, F, Hassanpour, S, Rahnema, M, Adeli, A and Ghashghayi, E 2017. Serotonin-induced hypophagia is mediated via α 2 and β 2 adrenergic receptors in neonatal layer-type chickens. British Poultry Science 58, 298–304.CrossRefGoogle ScholarPubMed
Zuidhof, MJ, Schneider, BL, Carney, VL, Korver, DR and Robinson, FE 2014. Growth, efficiency, and yield of commercial broilers from 1957, 1978, and 2005. Poultry Science 93, 2970–2982.CrossRefGoogle ScholarPubMed
Żyła, K, Duliński, R, Pierzchalska, M, Grabacka, M, Józefiak, D and Świątkiewicz, S 2013. Phytases and myo-inositol modulate performance, bone mineralization and alter lipid fractions in the serum of broilers. Journal of Animal and Feed Sciences 22, 56–62.CrossRefGoogle Scholar
Supplementary material: PDF

Gonzalez-Uarquin et al. supplementary material

Table S1

Download Gonzalez-Uarquin et al. supplementary material(PDF)
PDF 18.6 KB