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Detection of quantitative trait loci for porcine susceptibility to enterotoxigenic Escherichia coli F41 in a White Duroc × Chinese Erhualian resource population

Published online by Cambridge University Press:  01 July 2009

B. Yang ,
X. Huang ,
X. Yan ,
J. Ren ,
S. Yang ,
Z. Zou ,
W. Zeng ,
Y. Ou ,
W. Huang  and
L. Huang
B. Yang
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045, Nanchang, China
X. Huang
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045, Nanchang, China
X. Yan
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045, Nanchang, China
J. Ren
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045, Nanchang, China
S. Yang
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045, Nanchang, China
Z. Zou
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045, Nanchang, China
W. Zeng
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045, Nanchang, China
Y. Ou
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045, Nanchang, China
W. Huang
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045, Nanchang, China
L. Huang*
Affiliation:
Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045, Nanchang, China
*
*E-mail: Lushenghuang@hotmail.com
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Abstract

Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhoea in neonatal and postweaning pigs. F41 is one of ETEC fimbriae that adhere to the small intestinal epithelium and lead to development of diarrhoea. The genetic architecture of susceptibility to ETEC F41 remains elusive in pigs. In this study, we determined the in vitro adhesion phenotypes of ETEC F41 in a total of 835 F2 animals from a White Duroc × Erhualian intercross, and performed a genome scan using both F2 and half-sib analyses with 183 microsatellite markers to detect quantitative trait loci (QTL) for porcine susceptibility to ETEC F41. The two analyses consistently revealed a 1% genome-wide significant QTL on pig chromosome 4. Moreover, we determined F41 adhesion phenotypes in 14 purebred Erhualian and 14 White Duroc pigs. The results showed that both the founder breeds are segregating for the F41 adhesion phenotype, while less percentage of Erhualian pigs were adhesive to ETEC F41 compared to White Duroc pigs.

Keywords

ETEC F41susceptibilitypigQTL
Type
Full Paper
Information
animal , Volume 3 , Issue 7 , July 2009 , pp. 946 - 950
Copyright
Copyright © The Animal Consortium 2009

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Footnotes

a

These authors contributed equally to this study.

References

Baker, DR, Billey, LO, Francis, DH 1997. Distribution of K88 Escherichia coli-adhesive and nonadhesive phenotypes among pigs of four breeds. Veterinary Microbiology 54, 123–132.CrossRefGoogle ScholarPubMed
Bertschinger, HU, Bachmann, M, Mettler, C, Pospischil, A, Schraner, EM, Stamm, M, Sydler, T, Wild, P 1990. Adhesive fimbriae produced in vivo by Escherichia coli O139:K12(B):H1 associated with enterotoxaemia in pigs. Veterinary Microbiology 25, 267–281.CrossRefGoogle ScholarPubMed
Brooks, DE, Cavanagh, J, Jayroe, D, Janzen, J, Snoek, R, Trust, TJ 1989. Involvement of the MN blood group antigen in shear-enhanced hemagglutination induced by the Escherichia coli F41 adhesin. Infection and Immunity 57, 377–383.CrossRefGoogle ScholarPubMed
Chen, X, Gao, S, Jiao, XN, Liu, XF 2004. Prevalence of serogroups and virulence factors of Escherichia coli strains isolated from pigs with postweaning diarrhoea in eastern China. Veterinary Microbiology 103, 13–20.CrossRefGoogle ScholarPubMed
Churchill, GA, Doerge, RW 1994. Empirical threshold values for quantitative trait mapping. Genetics 138, 963–971.CrossRefGoogle ScholarPubMed
Duchet-Suchaux, MF, Bertin, AM, Menanteau, PS 1991. Susceptibility of Chinese Meishan and European Large White pigs to enterotoxigenic Escherichia coli strains bearing colonisation factor K88, 987P, K99, or F41. American Journal of Veterinary Research 52, 40–44.CrossRefGoogle ScholarPubMed
Gibbons, RA, Sellwood, R, Burrows, M, Hunter, PA 1977. Inheritance of resistance to neonatal E. colidiarrhoea in the pig: examination of the genetic system. Theoretical and Applied Genetics 81, 65–70.CrossRefGoogle Scholar
Green, P, Falls, K, Crooks, S 1990. Documentation for CRI-MAP, version 2.4. Washington University School of Medicine, St louis, MO, USA.Google Scholar
Guo, YM, Mao, HR, Ren, J, Yan, XM, Duan, YY, Yang, GC, Ren, DR, Zhang, ZY, Yang, B, Ouyang, J, Brenig, B, Haley, CS, Huang, LS 2009. A comprehensive linkage map of the porcine genome from a large scale White Duroc × Erhualian resource population and evaluation of factors affecting recombination rates. Animal Genetics 40, 47–52.CrossRefGoogle Scholar
Haley, CS, Knott, SA, Elsen, JM 1994. Mapping quantitative trait loci in crosses between outbred lines using least squares. Genetics 136, 1195–1207.CrossRefGoogle ScholarPubMed
Harduin-Lepers, A, Mollicone, R, Delannoy, P, Oriol, R 2005. The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach. Glycobiology 15, 805–817.CrossRefGoogle Scholar
Joller, D, Jørgensen, CB, Bertschinger, HU, Bürgi, E, Stannarius, C, Mortensen, PK, Cirera, S, Archibald, A, Genini, S, Edfors-lilja, I, Andersson, L, Fredholm, M, Vögeli, P 2006. Refined linkage mapping of the Escherichia coli F4ac receptor gene on pig chromosome 13. In Proceedings of the 30th International Conference on Animal Genetics, Brazil, Poster ID B512.Google Scholar
Jones, GW, Rutter, JM 1972. Role of the K88 antigen in the pathogenesis of neonatal diarrhoea caused by Escherichia coli in piglets. Infection and Immunity 6, 918–927.CrossRefGoogle ScholarPubMed
Knott, SA, Elsen, JM, Haley, CS 1996. Methods for multiple-marker mapping of quantitative trait loci in half-sib populations. Theoretical and Applied Genetics 93, 71–80.CrossRefGoogle ScholarPubMed
Lindahl, M 1989. Binding of F41 and K99 fimbriae of enterotoxigenic Escherichia coli to glycoproteins from bovine and porcine colostrums. Microbiology and Immunology 33, 373–379.CrossRefGoogle Scholar
Meijerink, E, Neuenschwander, S, Fries, R, Dinter, A, Bertschinger, HU, Stranzinger, G, Vogeli, P 2000. A DNA polymorphism influencing alpha(1,2)fucosyltransferase activity of the pig FUT1 enzyme determines susceptibility of small intestinal epithelium to Escherichia coli F18 adhesion. Immunogenetics 52, 129–136.CrossRefGoogle ScholarPubMed
Moon, HW, Nagy, B, Isaacson, RE, Orskov, I 1977. Occurrence of K99 antigen on Escherichia coli isolated from pigs and colonization of pig ileum by K99+ enterotoxigenic E. coli from calves and pigs. Infection and Immunity 15, 614–620.CrossRefGoogle ScholarPubMed
Moon, HW, Hoffman, LJ, Cornick, NA, Booher, SL, Bosworth, BT 1999. Prevalences of some virulence genes among Escherichia coli isolates from swine presented to a diagnostic laboratory in Iowa. Journal of Veterinary Diagnostic Investigation 11, 557–560.CrossRefGoogle ScholarPubMed
Morris, JA, Thorns, CJ, Wells, GA, Scott, AC, Sojka, WJ 1983. The production of F41 fimbriae by piglet strains of enterotoxigenic Escherichia coli that lack K88, K99 and 987P fimbriae. Journal of General Microbiology 129, 2753–2759.Google ScholarPubMed
Nagy, B, Moon, HW, Isaacson, RE 1977. Colonization of porcine intestine by enterotoxigenic Escherichia coli: selection of piliated forms in vivo, adhesion of piliated forms to epithelial cells in vitro, and incidence of a pilus antigen among porcine enteropathogenic E. coli. Infection and Immunity 16, 344–352.CrossRefGoogle ScholarPubMed
Peng, QL, Ren, J, Yan, XM, Huang, X, Tang, H, Wang, YZ, Zhang, B, Huang, LS 2007. The g.243A > G mutation in intron 17 of MUC4 is significantly associated with susceptibility/resistance to ETEC F4ab/ac infection in pigs. Animal Genetics 38, 397–400.CrossRefGoogle ScholarPubMed
Python, P, Jorg, H, Neuenschwander, S, Asai-Coakwell, M, Hagger, C, Burgi, E, Bertschinger, HU, Stranzinger, G, Vogeli, P 2005. Inheritance of the F4ab, F4ac and F4ad E. coli receptors in swine and examination of four candidate genes for F4acR. Journal of Animal Breeding and Genetics 122 (suppl. 1), 5–14.CrossRefGoogle ScholarPubMed
Seaton, G, Haley, CS, Knott, SA, Kearsey, M, Visscher, PM 2002. QTL express: mapping quantitative trait loci in simple and complex pedigrees. Bioinformatics 18, 339–340.CrossRefGoogle ScholarPubMed
Takashima, S, Tachida, Y, Nakagawa, T, Hamamoto, T, Tsuji, S 1999. Quantitative analysis of expression of mouse sialyltransferase genes by competitive PCR. Biochemical and Biophysical Research Communication 260, 23–27.CrossRefGoogle ScholarPubMed
Visscher, PM, Thompson, R, Haley, CS 1996. Confidence intervals in QTL mapping by bootstrapping. Genetics 143, 1013–1020.CrossRefGoogle ScholarPubMed
Yan, XM, Ren, J, Huang, X, Zhang, ZY, Ouyang, J, Zeng, WH, Zou, ZZ, Yang, SJ, Yang, B, Huang, LS 2009. Comparison of production traits between pigs with and without the Escherichia coli F4 receptors in a White Duroc × Erhualian intercross F2 population. Journal of Animal Science 87, 334–339.CrossRefGoogle Scholar
Yang, GC, Ren, J, Li, SJ, Mao, HR, Guo, YM, Zou, ZZ, Ren, DR, Ma, JW, Huang, LS 2008. Genome-wide identification of QTL for age at puberty in gilts using a large intercross F2 population between White Duroc × Erhualian. Genetics Selection Evolution 40, 529–539.Google Scholar
Zhang, B, Ren, J, Yan, XM, Huang, X, Ji, HY, Peng, Q, Zhang, ZY, Huang, LS 2008. Investigation of the porcine MUC13 gene: isolation, expression, polymorphisms and strong association with susceptibility to enterotoxigenic Escherichia coli F4ab/ac. Animal Genetics 39, 258–266.CrossRefGoogle ScholarPubMed