Skip to main content Accessibility help
×
Home

Comparative testis proteome of cattleyak from different developmental stages

  • L. Sun (a1), T. D. Mipam (a2), F. Zhao (a1), W. Liu (a1), W. Zhao (a1), S. Wu (a1), C. Xu (a1), S. Yu (a3) and X. Cai (a1)...

Abstract

Cattleyak (hybrid of cattle and yak) exhibit higher capability in adaptability and production than cattle and yak, while the infertility of F1 males greatly restricts the effective utilization of this hybrid and little progress has been made on investigating the mechanisms of the cattleyak infertility. Cattleyak individuals at three development stages (10, 12 and 14-month old) were sampled in this work and the isobaric tag for relative and absolute quantification method was employed to identify differences between their testicular proteomes. The proteomic analysis identified 318 proteins differentially expressed with significance at 12-month stage and 327 at 14-month compared with 10-month stage, respectively. Compared with the testicular proteome from 10-month cattleyak, the gene ontology (GO) annotations of the differentially expressed proteins at 12 months did not indicate significant differences from those at 14 months, which confirmed the histological observation that germ cell reduction was more obvious and spermatogenic arrest may become more serious in 12-month-old cattleyak. On the other hand, 56 differentially expressed proteins were coexpressed at 12 and 14-month stage compared with 10-month stage, in which 32 proteins were upregulated and 24 downregulated. GO analysis revealed that most of the differently expressed proteins were involved in the molecular function of catalytic activity, transporter activity, oxidoreductase activity and protein binding. Further analysis indicated that the differently expressed proteins including testis-expressed protein 101 precursor, RNA-binding motif protein, X chromosome, putative RNA-binding protein 3, heparin-binding proteins, tudor domain-containing protein 1, glutathione S-transferases (GSTA2, GSTP1), heat shock-related 70 kDa protein 2, estradiol 17-β-dehydrogenase11, 2,4-dienoyl-CoA reductase and peroxiredoxin-2 were possibly associated with testis development and spermatogenesis, which could be selected as candidate proteins in future study to examine the mechanisms of cattleyak infertility.

Copyright

Corresponding author

Footnotes

Hide All
a

These two authors contributed equally to this work.

Footnotes

References

Hide All
Amery, L, Mannaerts, GP, Subramani, S, Van Veldhoven, PP and Fransen, M 2001. Identification of a novel human peroxisomal 2, 4-dienoyl-CoA reductase related protein using the M13 phage protein VI phage display technology. Combinatorial Chemistry & High Throughput Screening 4, 545552.
Bellin, ME, Hawkins, HE, Oyarzo, JN, Vanderboom, RJ and Ax, RL 1996. Monoclonal antibody detection of heparin-binding proteins on sperm corresponds to increased fertility of bulls. Journal of Animal Science 74, 173182.
Bellin, ME, Oyarzo, JN, Hawkins, HE, Zhang, H, Smith, RG, Forrest, DW, Sprott, LR and Ax, RL 1998. Fertility-associated antigen on bull sperm indicates fertility potential. Journal of Animal Science 76, 20322039.
Cai, X, Mipam, TD, Zhao, FF and Sun, L 2015. SNPs detected in the yak MC4R gene and their association with growth traits. Animal 9, 10971103.
Chai, Z, Brereton, P, Suzuki, T, Sasano, H, Obeyesekere, V, Escher, G, Saffery, R, Fuller, P, Enriquez, C and Krozowski, Z 2003. 17 beta-hydroxysteroid dehydrogenase type XI localizes to human steroidogenic cells. Endocrinology 144, 20842091.
Chuma, S, Hosokawa, M, Kitamura, K, Kasai, S, Fujioka, M, Hiyoshi, M, Takamune, K, Noce, T and Nakatsuji, N 2006. Tdrd1/Mtr-1, a tudor-related gene, is essential for male germ-cell differentiation and nuage/germinal granule formation in mice. Proceedings of the National Academy of Sciences of the United States of America 103, 1589415899.
Faulkner, S, Elia, G, Mullen, MP, O’Boyle, P, Dunn, MJ and Morris, D 2012. A comparison of the bovine uterine and plasma proteome using iTRAQ proteomics. Proteomics 12, 20142023.
Fu, W, Li, CX, Liu, WJ, Ma, XQ, Ren, L, Huang, L, Jin, SY, Lin, YQ and Zheng, YC 2014. Identification of the differentially expressed testis proteins between yak and cattleyak by two-dimensional electrophoresis combined with mass spectrometry. Journal of Southwest University for Nationalities 40, 1115. (in Chinese with English abstract).
Fujihara, Y, Tokuhiro, K, Muro, Y, Kondoh, G, Araki, Y, Ikawa, M and Okabe, M 2013. Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa. Proceedings of the National Academy of Sciences of United States of America 110, 81118116.
Geissler, WM, Davis, DL, Wu, L, Bradshaw, KD, Patel, S, Mendonca, BB, Elliston, KO, Wilson, JD, Russell, DW and Andersson, S 1994. Male pseudohermaphroditism caused by mutations of testicular 17-hydroxysteroid dehydrogenase. Nature Genetics 7, 3439.
Guo, AP 1983. A comparative study on chromosomes of oxen (Bos taurus), yaks (Bos gronnieus) and their hybrids. Acta Genatica Sinica 10, 137143. (in Chinese with English abstract).
Hering, DM, Lecewicz, M, Kordan, W, Majewska, A and Kaminski, S 2015. Missense mutation in glutathione-S-transferase M1 gene is associated with sperm motility and ATP content in frozen-thawed semen of Holstein-Friesian bulls. Animal Reproduction Science 159, 9497.
Hu, OM, Zhong, JC, Chen, ZH and Cai, L 2000. A study on relationship between synaptonemal complex and male sterility of cattleyak. Journal of Southwest Nationalities College 26, 6166. (in Chinese with English abstract).
Ishizuka, T, Iizasa, T, Taira, M, Ishijima, S, Sonoda, T, Shimada, H, Nagatake, N and Tatibana, M 1992. Promoter regions of the human X-linked housekeeping genes PRPS1 and PRPS2 encoding phosphoribosylpyrophosphate synthetase subunit I and II isoforms. Biochimica et Biophysica Acta 1130, 139148.
Koivuranta, KT, Hakkola, EH and Hiltunen, JK 1994. Isolation and characterization of cDNA for human 120 kDa mitochondrial 2,4-dienoyl-coenzyme A reductase. The Biochemical Journal 304, 787792.
Kumar, V, Hassan, MI, Kashav, T, Singh, TP and Yadav, S 2008. Heparin-binding proteins of human seminal plasma: purification and characterization. Molecular Reproduction and Development 75, 17671774.
Kumar, V, Hassan, MI, Tomar, AK, Kashav, T, Nautiyal, J, Singh, S, Singh, TP and Yadav, S 2009. Proteomic analysis of heparin-binding proteins from human seminal plasma: a step towards identification of molecular markers of male fertility. Journal of Biosciences 34, 899908.
Lei, HZ, Sun, FX, Xu, WB and Liu, ZK 1964. Observation of reproductive physiology and characteristics of yak. Chinese Journal of Animal Science 7, 13. (in Chinese).
Li, X, Li, QF, Zhao, XB, Xu, HT, Gu, Y, Zhu, X, Xie, Z and Liu, HL 2010. Sequence analysis and study on the expression level of Dmc1 mRNA in yak and cattleyak testis. Scientia Agricultura Sinica 43, 32213229. (in Chinese with English abstract).
Lu, JY and Zi, XD 2014. The study of anatomical structure of F1 generation cattle cattleyak testis. Animal Husbandry & Veterinary Medicine 46, 6163. (in Chinese with English abstract).
Ma, K, Inglis, JD, Sharkey, A, Bickmore, WA, Hill, RE, Prosser, EJ, Speed, RM, Thomson, EJ, Jobling, M and Taylor, K 1993. A Y chromosome gene family with RNA-binding protein homology: candidates for the azoospermia factor AZF controlling human spermatogenesis. Cell 75, 12871295.
Matsunaga, S, Takata, H, Morimoto, A, Hayashihara, K, Higashi, T, Akatsuchi, K, Mizusawa, E, Yamakawa, M, Ashida, M, Matsunaga, TM, Azuma, T, Uchiyama, S and Fukui, K 2015. RBMX: a regulator for maintenance and centromeric protection of sister chromatid cohesion. Cell Reports 1, 299308.
Miller, DJ, Winer, MA and Ax, RL 1990. Heparin-binding proteins from seminal plasma bind to bovine spermatozoa and modulate capacitation by heparin. Biology of Reproduction 42, 899915.
Miyamoto, N, Izumi, H, Miyamoto, R, Kubota, T, Tawara, A, Sasaguri, Y and Kohno, K 2009. Nipradilol and timolol induce Foxo3a and peroxiredoxin 2 expression and protect trabecular meshwork cells from oxidative stress. Investigative Ophthalmology & Visual Science 50, 27772784.
Moghrabi, N, Head, JR and Andersson, S 1997. Cell type-specific expression of 17-hydroxysteroid dehydrogenase type 2 in human placenta and fetal liver. Journal of Clinical Endocrinology and Metabolism 82, 38723878.
Pan, ZX, Liu, ZS, Li, YX, Yu, SL, Li, MG, Xie, Z and Li, QF 2010. Difference of SNRPN methylation status and its mRNA expression in testes between cattleyak and their parent. Scientia Agricultura Sinica 43, 47094716. (in Chinese with English abstract).
Pereira, SB, Ow, SY, Barrios-Llerena, ME, Wright, PC, Moradas-Ferreira, P and Tamagnini, P 2011. iTRAQ-based quantitative proteomic analysis of Gloeothece sp. PCC 6909: comparison with its sheathless mutant and adaptations to nitrate deficiency and sulfur limitation. Journal of Proteomics 75, 270283.
Peskin, AV, Low, FM, Paton, LN, Maghzal, GJ, Hampton, MB and Winterbourn, CC 2007. The high reactivity of peroxiredoxin 2 with H2O2 is not reflected in its reaction with other oxidants and thiol reagents. Journal of Biological Chemistry 282, 1188511892.
Pottiez, G, Wiederin, J, Fox, HS and Ciborowski, P 2012. Comparison of 4-plex to 8-plex iTRAQ quantitative measurements of proteins in human plasma samples. Journal of Proteome Research 11, 37743781.
Redgrove, KA, Nixon, B, Baker, MA, Hetherington, L, Baker, G, Liu, DY and Aitken, RJ 2012. The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognition. PLoS One 7, e50851.
Wang, J, Saxe, JP, Tanaka, T, Chuma, S and Lin, H 2009. Mili interacts with tudor domain-containing protein 1 in regulating spermatogenesis. Current Biology 19, 640644.
Wu, WW, Wang, G, Baek, SJ and Shen, RF 2006. Comparative study of three proteomic quantitative methods, DIGE, cICAT, and iTRAQ, using 2D gel- or LC-MALDI TOF/TOF. Journal of Proteome Research 5, 651658.
Zeng, XB, Chai, ZX, Wang, Y, Ma, ZJ, Yang, Q, Song, QQ and Zhong, JC 2014. Comparative transcriptome analysis of spermatogenesis arrest in cattleyak. Scientia Sinica 44, 584601. (in Chinese with English abstract).
Zhou, Y, Luo, H, Li, BJ, Jia, C, Xie, Z, Zhao, XB, Zhong, JC and Li, QF 2013. mRNA expression level and promoter methylation of DDX4 gene in testes of yak and cattleyak. Scientia Agricultura Sinica 3, 630638. (in Chinese with English abstract).

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed