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12 - Transcriptomes in schizophrenia: assessing altered gene expression with microarrays

Published online by Cambridge University Press:  04 August 2010

By
David A. Lewis ,
Karoly Mirnics  and
Pat Levitt
Edited by
Matcheri S. Keshavan ,
James L. Kennedy  and
Robin M. Murray
David A. Lewis
Affiliation:
University of Pittsburgh, Pittsburgh, USA
Karoly Mirnics
Affiliation:
University of Pittsburgh, Pittsburgh, USA
Pat Levitt
Affiliation:
Vanderbilt University, Nashville, USA
Matcheri S. Keshavan
Affiliation:
University of Pittsburgh
James L. Kennedy
Affiliation:
Clarke Institute of Psychiatry, Toronto
Robin M. Murray
Affiliation:
Institute of Psychiatry, London
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Summary

This chapter reviews the current status of the technology to assess transcriptomes, its associated strengths and limitations, and initial findings resulting from the use of the approaches to study schizophrenia. Two types of microarray platform have been used in studies of schizophrenia: complementary DNA (cDNA) microarrays and synthetic oligonucleotide probe arrays. The study of gene expression in human brain tissue, the area of interest in schizophrenia, requires the use of postmortem human brain specimens. In postmortem brain specimens from subjects with schizophrenia, a difference in the expression level of one or a cluster of genes may have several different meanings. The application of transcriptome-based methods to studies of the molecular neuropathology of schizophrenia is clearly in their infancy, and like newborns they offer great promise for the future. However, careful rearing is essential to fulfill this promise.

Keywords

altered gene expressionDNAmicroarraystranscriptomessynthetic oligonucleotide probe arraysschizophrenia
Type
Chapter
Information
Neurodevelopment and Schizophrenia , pp. 210 - 223
Publisher: Cambridge University Press
Print publication year: 2004

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