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Chapter 7 - Handling of Gametes and Embryos

Published online by Cambridge University Press:  26 April 2023

By
Sharon T. Mortimer
Edited by
Catherine Racowsky ,
Jacques Cohen  and
Nicholas Macklon
Catherine Racowsky
Affiliation:
Hôpital Foch, France
Jacques Cohen
Affiliation:
IVF 2.0, New York
Nicholas Macklon
Affiliation:
London Women's Clinic
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Summary

This chapter provides an overview of the laboratory systems required for effective handling and processing of spermatozoa, oocytes and embryos for ART. These systems include the laboratory environment, and management of exposure to volatile organic compounds, as well as the culture environment, including temperature, pH, CO2 and O2, osmolarity, culture medium, and contact materials. Other considerations in effective gamete and handling, such as sample identification and witnessing, are also covered.

Type
Chapter
Information
Manual of Embryo Selection in Human Assisted Reproduction , pp. 62 - 69
Publisher: Cambridge University Press
Print publication year: 2023

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References

Mortimer, D, Cohen, J, Mortimer, ST, Fawzy, M, McCulloh, DH, Morbeck, DE, et al. Cairo consensus on the IVF laboratory environment and air quality: report of an expert meeting. Reprod Biomed Online. 2018;36:658–74.Google Scholar
Cairo 2018 Consensus Group. “There is only one thing that is truly important in an IVF lab: everything”: Cairo consensus guidelines on IVF culture conditions. Reprod Biomed Online. 2020;40:33–60.Google Scholar
Gardner, DK, Lane, M. Alleviation of the ‘2-cell block’ and development to the blastocyst of CFI mouse embryos: role of amino acids, EDTA and physical parameters. Hum Reprod. 1996;11:2703–12.Google Scholar
Morbeck, DE, Paczkowski, M, Fredrickson, JR, Krisher, RL, Hoff, HS, Baumann, NA, et al. Composition of protein supplements used for human embryo culture. J Assist Reprod Genet. 2014;31:1703–11.Google Scholar
Mortimer, ST, Mortimer, D. Quality and risk management in the IVF laboratory. 2nd ed. Cambridge, UK: Cambridge University Press; 2015.Google Scholar
ESHRE Guideline Group on Good Practice in IVF Labs, De los Santos, MJ, Apter, S, Coticchio, G, Debrock, S, Lundin, K, et al. Revised guidelines for good practice in IVF laboratories (2015). Hum Reprod. 2016;31:685–6.Google Scholar
Mortimer, D. The functional anatomy of the human spermatozoon: relating ultrastructure and function. Mol Hum Reprod. 2018;24:567–92.Google Scholar
Mortimer, D. Sperm preparation methods. J Androl. 2000;21:357–66.Google Scholar
World Health Organization. WHO laboratory manual for the examination and processing of human semen. 5th ed. Geneva: World Health Organization; 2010.Google Scholar
Mortimer, D, Mortimer, ST. Density gradient separation of sperm for artificial insemination. In: Carrell, DT, Aston, KI, editors, Spermatogenesis and spermiogenesis: methods and protocols. Methods in Molecular Biology 927, New York: Springer (Humana Press); 2013. p. 217–26.Google Scholar
ESHRE Special Interest Group Embryology and Alpha Scientists in Reproductive Medicine. The Vienna consensus workshop on laboratory key performance indicators. Hum Reprod Open. 2017(2), doi.org/10.1093/ hropen/hox011, and Reprod Biomed Online 2017;35:494–510 (simultaneous publication).Google Scholar
Björndahl, L, Mortimer, D, Barratt, CLR, Castilla, JA, Menkveld, R, Kvist, U, et al. A practical guide to basic laboratory andrology. Cambridge, UK: Cambridge University Press; 2010. p. 219–25.Google Scholar
Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology. The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum Reprod. 2011;26:1270–83 and Reprod Biomed Online 2011;22:632–46 (simultaneous publication).Google Scholar
Wale, PL, Gardner, DK. The effects of chemical and physical factors on mammalian embryo culture and their importance for the practice of assisted human reproduction. Hum Reprod Update. 2016;22:2–22.Google Scholar

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