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9 - CNT and proteins for bioelectronics in personalized medicine

from Part II - Biosensors

Published online by Cambridge University Press:  05 September 2015

By
Andrea Cavallini ,
Cristina Boero ,
Giovanni De Micheli  and
Sandro Carrara
Edited by
Sandro Carrara  and
Krzysztof Iniewski
Andrea Cavallini
Affiliation:
École Polytechnique Fédérale de Lausanne
Cristina Boero
Affiliation:
École Polytechnique Fédérale de Lausanne
Giovanni De Micheli
Affiliation:
École Polytechnique Fédérale de Lausanne
Sandro Carrara
Affiliation:
EPFL, Lausanne, Switzerland
Sandro Carrara
Affiliation:
École Polytechnique Fédérale de Lausanne
Krzysztof Iniewski
Affiliation:
Redlen Technologies Inc., Canada
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Summary

From their discovery, CNTs have increasingly attracted interest because of their peculiar electrical, mechanical, and chemical properties. In 1991, Sumio Iijima first observed and described in detail the atomic arrangement of this new type of carbon structure [1]. By a technique used for fullerene synthesis, he produced needle-like tubes at the cathode of an arc-discharge evaporator. From that time, carbon nanotubes have been used for many applications and represent one of the most typical building blocks used in nanotechnology. Their peculiarities include unique properties of field emission and electronic transport, higher mechanical strength with respect to other materials, and interesting chemical features.

The use of CNTs has recently gained momentum in the development of electrochemical biosensors, since their utilization can create devices with enhanced sensitivity and detection limit capable of detecting compounds in concentrations comparable to those present in the human body.

This chapter will review the most important features of carbon nanotubes, and present an example in which their application can enhance the detection of drugs and metabolites relevant in personalized medicine: P450 biosensors for therapeutic drug monitoring.

Overview

Carbon is a very interesting element, since it can assume several stable molecular structures. Any molecule entirely composed of carbon is called a fullerene.

Type
Chapter
Information
Handbook of Bioelectronics
Directly Interfacing Electronics and Biological Systems
 , pp. 109 - 121
Publisher: Cambridge University Press
Print publication year: 2015

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References

Iijima, S., 1991. Helical microtubules of graphitic carbon. Nature 354(6348), 56–58.CrossRefGoogle Scholar
Kroto, H.W., Heath, J.R., O’Brien, S.C., Curl, R.F., Smalley, R.E., 1985. C 60: buckminsterfullerene. Nature 318(6042), 162–163.CrossRefGoogle Scholar
Gooding, J.J., 2005. Nanostructuring electrodes with carbon nanotubes: A review on electrochemistry and applications for sensing. Electrochimica Acta 50(15), 3049–3060.CrossRefGoogle Scholar
Krupke, R., Hennrich, F., Löhneysen, H.v., Kappes, M.M., 2003. Separation of metallic from semiconducting single-walled carbon nanotubes. Science 301(5631), 344–347.CrossRefGoogle ScholarPubMed
Chattopadhyay, D., Galeska, I., Papadimitrakopoulos, F., 2003. A route for bulk separation of semiconducting from metallic single-wall carbon nanotubes. Journal of the American Chemical Society 125(11), 3370–3375.CrossRefGoogle ScholarPubMed
Gong, K., Yan, Y., Zhang, M., et al. 2005. Electrochemistry and electroanalytical applications of carbon nanotubes: a review. Analytical Sciences 21(12), 1383–1393.CrossRefGoogle ScholarPubMed
Wong, H-S.P., Akinwande, D., Carbon Nanotube and Graphene Device Physics. Cambridge University Press, 2010.CrossRefGoogle Scholar
Dai, H., 2002. Carbon nanotubes: synthesis, integration, and properties. Accounts of Chemical Research 35(12), 1035–1044.CrossRefGoogle ScholarPubMed
Niyogi, S., Hamon, M., Hu, H., et al. 2002. Chemistry of single-walled carbon nanotubes. Accounts of Chemical Research 35(12), 1105–1113.CrossRefGoogle ScholarPubMed
Chou, A., Böcking, T., Singh, N.K., Gooding, J.J., 2005. Demonstration of the importance of oxygenated species at the ends of carbon nanotubes for their favourable electrochemical properties. Chemical Communications 7, 842–844.CrossRefGoogle Scholar
Ando, T., 2009. The electronic properties of graphene and carbon nanotubes. NPG Asia Materials 1(1), 17–21.CrossRefGoogle Scholar
Harris, P.J., Carbon Nanotubes and Related Structures. Cambridge University Press, 2001.Google Scholar
Saito, R., Dresselhaus, G., Dresselhaus, M.S., Physical Properties of Carbon Nanotubes. Imperial College Press, 1998.CrossRefGoogle Scholar
Li, J., Cassell, A., Delzeit, L., Han, J., Meyyappan, M., 2002. Novel three-dimensional electrodes: electrochemical properties of carbon nanotube ensembles. Journal of Physical Chemistry B 106(36), 9299–9305.CrossRefGoogle Scholar
Azamian, B.R., Davis, J.J., Coleman, K.S., Bagshaw, C.B., Green, M.L., 2002. Bioelectrochemical single-walled carbon nanotubes. Journal of the American Chemical Society 124(43), 12664–12665.CrossRefGoogle ScholarPubMed
Taurino, I., Carrara, S., Giorcelli, M., Tagliaferro, A., De Micheli, G., 2011b. Comparing sensitivities of differently oriented multi-walled carbon nanotubes integrated on silicon wafer for electrochemical biosensors. Sensors and Actuators B: Chemical 160(1), 327–333.CrossRefGoogle Scholar
Musameh, M., Wang, J., Merkoci, A., Lin, Y., 2002. Low-potential stable NADH detection at carbon-nanotube-modified glassy carbon electrodes. Electrochemistry Communications 4(10), 743–746.CrossRefGoogle Scholar
Wang, J., Kawde, A-N., Musameh, M., 2003a. Carbon-nanotube-modified glassy carbon electrodes for amplified label-free electrochemical detection of DNA hybridization. Analyst 128(7), 912–916.CrossRefGoogle ScholarPubMed
Wu, K., Sun, Y., Hu, S., 2003. Development of an amperometric indole-3-acetic acid sensor based on carbon nanotubes film coated glassy carbon electrode. Sensors and Actuators B: Chemical 96(3), 658–662.CrossRefGoogle Scholar
Zhang, J., Gao, L., 2007. Dispersion of multiwall carbon nanotubes by sodium dodecyl sulfate for preparation of modified electrodes toward detecting hydrogen peroxide. Materials Letters 61(17), 3571–3574.CrossRefGoogle Scholar
Moore, R.R., Banks, C.E., Compton, R.G., 2004. Basal plane pyrolytic graphite modified electrodes: comparison of carbon nanotubes and graphite powder as electrocatalysts. Analytical Chemistry 76(10), 2677–2682.CrossRefGoogle ScholarPubMed
Wang, J., Li, M., Shi, Z., Li, N., Gu, Z., 2002. Direct electrochemistry of cytochrome c at a glassy carbon electrode modified with single-wall carbon nanotubes. Analytical Chemistry 74(9), 1993–1997.CrossRefGoogle Scholar
Britto, P., Santhanam, K., Ajayan, P., 1996. Carbon nanotube electrode for oxidation of dopamine. Bioelectrochemistry and Bioenergetics 41(1), 121–125.CrossRefGoogle Scholar
Boero, C., Carrara, S., Del Vecchio, G., Calzà, L., De Micheli, G., 2011a. Highly sensitive carbon nanotube-based sensing for lactate and glucose monitoring in cell culture. NanoBioscience, IEEE Transactions on 10(1), 59–67.CrossRefGoogle ScholarPubMed
Taurino, I., Carrara, S., Giorcelli, M., Tagliaferro, A., De Micheli, G., 2011a. Comparing sensitivities of differently oriented multi-walled carbon nanotubes integrated on silicon wafer for electrochemical biosensors. Sensors and Actuators B: Chemical. 160(1), 327–333.CrossRefGoogle Scholar
Withey, G., Lazareck, A., Tzolov, M., et al. 2006. Ultra-high redox enzyme signal transduction using highly ordered carbon nanotube array electrodes. Biosensors and Bioelectronics 21(8), 1560–1565.CrossRefGoogle ScholarPubMed
Liu, Z., Shen, Z., Zhu, T. et al. 2000. Organizing single-walled carbon nanotubes on gold using a wet chemical self-assembling technique. Langmuir 16(8), 3569–3573.CrossRefGoogle Scholar
Gooding, J.J., Wibowo, R., Liu, J., et al. 2003. Protein electrochemistry using aligned carbon nanotube arrays. Journal of the American Chemical Society 125(30), 9006–9007.CrossRefGoogle ScholarPubMed
Valentini, F., Amine, A., Orlanducci, S., Terranova, M.L., Palleschi, G., 2003. Carbon nanotube purification: preparation and characterization of carbon nanotube paste electrodes. Analytical Chemistry 75(20), 5413–5421.CrossRefGoogle ScholarPubMed
Wang, J., Musameh, M., 2004. Carbon nanotube screen-printed electrochemical sensors. Analyst 129(1), 1–2.CrossRefGoogle ScholarPubMed
Gao, M., DAL, L., Wallace, G., 2003. Glucose sensors based on glucose-oxidase-containing polypyrrole/aligned carbon nanotube coaxial nanowire electrodes. Synthetic Metals 137(1–3), 1393–1394.CrossRefGoogle Scholar
Dhand, C., Arya, S.K., Datta, M., Malhotra, B., 2008. Polyaniline–carbon nanotube composite film for cholesterol biosensor. Analytical Biochemistry 383(2),194–199.CrossRefGoogle ScholarPubMed
Wang, J., Musameh, M., Lin, Y., 2003b. Solubilization of carbon nanotubes by Nafion toward the preparation of amperometric biosensors. Journal of the American Chemical Society 125(9), 2408–2409.CrossRefGoogle ScholarPubMed
Tsai, Y-C., Li, S-C., Chen, J-M., 2005. Cast thin film biosensor design based on a nafion backbone, a multiwalled carbon nanotube conduit, and a glucose oxidase function. Langmuir 21(8), 3653–3658.CrossRefGoogle Scholar
Cavallini, A., De Micheli, G., Carrara, S., 2011. Comparison of three methods of biocompatible multi-walled carbon nanotubes confinement for the development of implantable amperometric adenosine-5-triphosphate biosensors. Sensor Letters 9(5), 1838–1844.CrossRefGoogle Scholar
Deng, C., Chen, J., Nie, Z., Si, S., 2010. A sensitive and stable biosensor based on the direct electrochemistry of glucose oxidase assembled layer-by-layer at the multiwall carbon nanotube-modified electrode. Biosensors and Bioelectronics 26(1),213–219.CrossRefGoogle ScholarPubMed
Wang, Y., Wang, X., Wu, B. et al. 2008. Dispersion of single-walled carbon nanotubes in poly (diallyldimethylammonium chloride) for preparation of a glucose biosensor. Sensors and Actuators B: Chemical 130(2), 809–815.CrossRefGoogle Scholar
Smart, S., Cassady, A., Lu, G., Martin, D., 2006. The biocompatibility of carbon nanotubes. Carbon 44(6), 1034–1047.CrossRefGoogle Scholar
Hussain, M., Kabir, M., Sood, A., 2009. On the cytotoxicity of carbon nanotubes. Current Science 96(5), 664–673.Google Scholar
de Montellano, P.R.O., Cytochrome P450: Structure, Mechanism, and Biochemistry. Springer, 2004.Google Scholar
Werck-Reichhart, D., Feyereisen, R., 2000. Cytochromes P450: a success story. Genome Biology 1(6), 3003.3001–3003.3009.CrossRefGoogle ScholarPubMed
Guengerich, F.P., 2007. Cytochrome P450 and chemical toxicology. Chemical Research in Toxicology 21(1), 70–83.CrossRefGoogle ScholarPubMed
Guengerich, F.Human cytochrome P450 enzymes. Cytochrome P450, pp. 377–530. Plenum, 2005.Google Scholar
Johnson, D., Lewis, B., Elliot, D., Miners, J., Martin, L., 2005. Electrochemical characterisation of the human cytochrome P450 CYP2C9. Biochemical Pharmacology 69(10), 1533–1541.CrossRefGoogle ScholarPubMed
Bard, A.J., Faulkner, L.R., Electrochemical Methods: Fundamentals and Applications. Wiley,1980.Google Scholar
Armstrong, F.A., Heering, H.A., Hirst, J., 1997. Reaction of complex metalloproteins studied by protein-film voltammetry. Chemical Society Reviews 26(3), 169–179.CrossRefGoogle Scholar
Carrara, S., Cavallini, A., Erokhin, V., De Micheli, G., 2011. Multi-panel drugs detection in human serum for personalized therapy. Biosensors and Bioelectronics 26(9), 3914–3919.CrossRefGoogle ScholarPubMed
Smith, A., Datta, S.P., Smith, G.H. et al. Oxford Dictionary of Biochemistry and Molecular Biology. Oxford University Press, 2000.Google Scholar
Asuri, P., Bale, S.S., Pangule, R.C. et al. 2007. Structure, function, and stability of enzymes covalently attached to single-walled carbon nanotubes. Langmuir 23(24), 12318–12321.CrossRefGoogle ScholarPubMed
Lyons, M.E., 2008. Carbon nanotube based modified electrode biosensors. Part 1. Electrochemical studies of the flavin group redox kinetics at SWCNT/glucose oxidase composite modified electrodes. International Journal of Electrochemical Science 3, 819–853.Google Scholar
Juma, F., Rogers, H., Trounce, J., 2012. Pharmacokinetics of cyclophosphamide and alkylating activity in man after intravenous and oral administration. British Journal of Clinical Pharmacology 8(3), 209–217.CrossRefGoogle Scholar
Baj-Rossi, C., Micheli, G.D., Carrara, S., 2012. Electrochemical detection of anti-breast-cancer agents in human serum by cytochrome P450-coated carbon nanotubes. Sensors 12(5), 6520–6537.CrossRefGoogle ScholarPubMed
Boero, C., Carrara, S., Del Vecchio, G., Calzà, L., De Micheli, G., 2011b. Targeting of multiple metabolites in neural cells monitored by using protein-based carbon nanotubes. Sensors and Actuators B: Chemical 157(1), 216–224.CrossRefGoogle Scholar
Carrara, S., Shumyantseva, V.V., Archakov, A.I., Samorì, B., 2008. Screen-printed electrodes based on carbon nanotubes and cytochrome P450scc for highly sensitive cholesterol biosensors. Biosensors and Bioelectronics 24(1), 148–150.CrossRefGoogle ScholarPubMed
Cavallini, A., Carrara, S., De Micheli, G., Erokhin, V., P450-mediated electrochemical sensing of drugs in human plasma for personalized therapy. Ph.D. Research in Microelectronics and Electronics (PRIME), 2010 Conference on, pp. 1–4. IEEE, 2010.
Gross, A.S., 2002. Best practice in therapeutic drug monitoring. British Journal of Clinical Pharmacology 46(2), 95–99.CrossRefGoogle Scholar
Hiemke, C., 2008. Clinical utility of drug measurement and pharmacokinetics–therapeutic drug monitoring in psychiatry. European Journal of Clinical Pharmacology 64(2), 159–166.CrossRefGoogle Scholar
Nicoll, D., McPhee, S.J., Pignone, M., Therapeutic drug monitoring: principles and interpretation. Pocket Guide to Diagnostic Tests, pp. 191–193. Lange Medical Books/McGraw-Hill, 2001.Google Scholar
Bonard, J-M., Kind, H., Stöckli, T., Nilsson, L-O., 2001. Field emission from carbon nanotubes: the first five years. Solid-State Electronics 45(6), 893–914.CrossRefGoogle Scholar

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